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The map label for this gene is pyrG

Identifier: 23100462

GI number: 23100462

Start: 3115553

End: 3117160

Strand: Reverse

Name: pyrG

Synonym: OB3007

Alternate gene names: 23100462

Gene position: 3117160-3115553 (Counterclockwise)

Preceding gene: 23100463

Following gene: 23100461

Centisome position: 85.86

GC content: 37.81

Gene sequence:

>1608_bases
GTGACAAAGTATATCTTCGTTACTGGAGGAGTAGTATCTTCTCTAGGTAAAGGAATAACAGCAGCATCTTTAGGTCGTTT
ACTGAAAAACCGTGGATTAAGTGTGACTATCCAAAAATTTGATCCATATATTAATGTGGATCCTGGAACAATGAGTCCAT
ATCAACATGGTGAAGTATTTGTAACACAAGATGGAGCGGAAACGGACTTAGACTTAGGTCATTATGAACGCTTTATTGAT
ATTAACTTGAACAAATACAGTAACATTACAACAGGAAAAGTTTATTCAAGCGTTATTCGTAAGGAACGTCGCGGAGATTA
TCTTGGTGGTACTGTTCAAGTAATTCCTCATATAACGAATGAGATAAAAGATCAAGTGTTTCGAGCAGGTGAAGCAACTG
GTGCAGATGTAGTAATTACAGAAATTGGTGGAACAGTTGGGGATATTGAATCTCTTCCATTCCTTGAAGCCATTCGTCAA
ATAAAAAGTGATGTGGGTCGTGACAGTGTTATGTATGTACATTGTACATTAGTTCCCTATATTAAAGCAGCTGGCGAAAT
GAAAACTAAACCAACCCAACACAGTGTTAAAGAATTACGTTCACTGGGAATTCAACCAGATGCGATTGTATTACGTACAG
AAATGGCGATAAGCAAAGATATGAAAGAAAAGATTGCTTTATTCTGTGATATTGATGATAAATCAGTTATTGAAATGCGT
GATGCGGACACACTTTACCAAGTACCAATCGCTCTACAAGAAGAAAAGCTAGATCAAATTACTTGTAATCATTTTGGACT
TGATTGTCCAGAAGCGGATATGGAAGAATGGAAAGGTCTTGTGAATAAGGTCCGTAACTTAAGTAAAACAACTCATATAG
GACTTGTTGGTAAGTATGTAGAACTTCCAGATGCTTATTTATCTGTTGTAGAGTCTTTAAAACATGCAGGATATGATTAT
GATACTGATGTGAAGATACATTGGATTAACTCGGAATTACTATCTGAAGAGCAAATTAAAGAGGAACTATCCAAAGTGGA
CGGTGTATTAGTTCCAGGTGGATTCGGAGATCGAGGTATTGATGGAAAAATTGAAGCGATTCGTTATGCTCGTGAGAACA
ATATTCCGTTCTTTGGTATATGCTTAGGAATGCAATTAGCTTCTGTAGAATTCGCTCGTAACGTGGTGGGTCTTTCTCAA
GCCCATTCAGCGGAAATCAATCCACATACACCGCATCCAGTCATTGATTTACTACCAGAACAAAAGGATATCGAGGATTT
AGGTGGTACCCTTCGTTTAGGTGCATATCCTTGTAAACTTGTTGATGGTACAAAAGCGAAAGAGGCATATGATGGTGCAG
ATGTTATTGAAGAAAGACATCGTCATCGTTATGAATTTAATAATGTATATAGAGATCAAATGGCGGAGAAAGGTTTTGTG
TTTTCTGGTACAAGCCCAGATGGTCGTTTAGTGGAAACAATTGAAGTGAAAGACCATCCGTGGTTTGTAGCTTGTCAATT
CCATCCAGAATTTACTTCACGCCCAACTCGTGCACAAAGCTTATTTAAAGGGTTTATTGGCGCTTCCGTCGACTATCGTA
AAGGTTAA

Upstream 100 bases:

>100_bases
TTTTCCCAGTTAAATGGGGGAACGCTTTTCTTATTTTATTTTCCCCTTTAGCCATACTAGGAATAATAAAATATCATAGA
TAGAAAGAAGGATGAAGAAC

Downstream 100 bases:

>100_bases
TAATTTTATCATAAAACTGCTCAAATTTTTATTGTTTGAGCAGTTTTTTGTATAGAGAATTAACAAATTTATGTGCAGTG
TATCACTATATTAGATGTTT

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFID
INLNKYSNITTGKVYSSVIRKERRGDYLGGTVQVIPHITNEIKDQVFRAGEATGADVVITEIGGTVGDIESLPFLEAIRQ
IKSDVGRDSVMYVHCTLVPYIKAAGEMKTKPTQHSVKELRSLGIQPDAIVLRTEMAISKDMKEKIALFCDIDDKSVIEMR
DADTLYQVPIALQEEKLDQITCNHFGLDCPEADMEEWKGLVNKVRNLSKTTHIGLVGKYVELPDAYLSVVESLKHAGYDY
DTDVKIHWINSELLSEEQIKEELSKVDGVLVPGGFGDRGIDGKIEAIRYARENNIPFFGICLGMQLASVEFARNVVGLSQ
AHSAEINPHTPHPVIDLLPEQKDIEDLGGTLRLGAYPCKLVDGTKAKEAYDGADVIEERHRHRYEFNNVYRDQMAEKGFV
FSGTSPDGRLVETIEVKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVDYRKG

Sequences:

>Translated_535_residues
MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFID
INLNKYSNITTGKVYSSVIRKERRGDYLGGTVQVIPHITNEIKDQVFRAGEATGADVVITEIGGTVGDIESLPFLEAIRQ
IKSDVGRDSVMYVHCTLVPYIKAAGEMKTKPTQHSVKELRSLGIQPDAIVLRTEMAISKDMKEKIALFCDIDDKSVIEMR
DADTLYQVPIALQEEKLDQITCNHFGLDCPEADMEEWKGLVNKVRNLSKTTHIGLVGKYVELPDAYLSVVESLKHAGYDY
DTDVKIHWINSELLSEEQIKEELSKVDGVLVPGGFGDRGIDGKIEAIRYARENNIPFFGICLGMQLASVEFARNVVGLSQ
AHSAEINPHTPHPVIDLLPEQKDIEDLGGTLRLGAYPCKLVDGTKAKEAYDGADVIEERHRHRYEFNNVYRDQMAEKGFV
FSGTSPDGRLVETIEVKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVDYRKG
>Mature_534_residues
TKYIFVTGGVVSSLGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVFVTQDGAETDLDLGHYERFIDI
NLNKYSNITTGKVYSSVIRKERRGDYLGGTVQVIPHITNEIKDQVFRAGEATGADVVITEIGGTVGDIESLPFLEAIRQI
KSDVGRDSVMYVHCTLVPYIKAAGEMKTKPTQHSVKELRSLGIQPDAIVLRTEMAISKDMKEKIALFCDIDDKSVIEMRD
ADTLYQVPIALQEEKLDQITCNHFGLDCPEADMEEWKGLVNKVRNLSKTTHIGLVGKYVELPDAYLSVVESLKHAGYDYD
TDVKIHWINSELLSEEQIKEELSKVDGVLVPGGFGDRGIDGKIEAIRYARENNIPFFGICLGMQLASVEFARNVVGLSQA
HSAEINPHTPHPVIDLLPEQKDIEDLGGTLRLGAYPCKLVDGTKAKEAYDGADVIEERHRHRYEFNNVYRDQMAEKGFVF
SGTSPDGRLVETIEVKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVDYRKG

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=45.985401459854, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=45.1553930530165, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=45.1553930530165, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=45.1553930530165, Blast_Score=482, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=543, Percent_Identity=55.8011049723757, Blast_Score=616, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=40.0332225913621, Blast_Score=425, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=43.5483870967742, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322563, Length=559, Percent_Identity=42.5760286225402, Blast_Score=456, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24664469, Length=549, Percent_Identity=45.7194899817851, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI21357815, Length=494, Percent_Identity=43.5222672064777, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_OCEIH (Q8EM53)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693929.1
- ProteinModelPortal:   Q8EM53
- SMR:   Q8EM53
- GeneID:   1016717
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3007
- NMPDR:   fig|221109.1.peg.3009
- HOGENOM:   HBG597806
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- BioCyc:   OIHE221109:OB3007-MONOMER
- BRENDA:   6.3.4.2
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 59551; Mature: 59420

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 381-381 ACT_SITE 508-508 ACT_SITE 510-510

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTQDGAETDLDLGHYERFIDINLNKYSNITTGKVYSSVIRKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
EIKDQVFRAGEATGADVVITEIGGTVGDIESLPFLEAIRQIKSDVGRDSVMYVHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
IKAAGEMKTKPTQHSVKELRSLGIQPDAIVLRTEMAISKDMKEKIALFCDIDDKSVIEMR
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCCEEEEC
DADTLYQVPIALQEEKLDQITCNHFGLDCPEADMEEWKGLVNKVRNLSKTTHIGLVGKYV
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHH
ELPDAYLSVVESLKHAGYDYDTDVKIHWINSELLSEEQIKEELSKVDGVLVPGGFGDRGI
HCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHCCEEECCCCCCCCC
DGKIEAIRYARENNIPFFGICLGMQLASVEFARNVVGLSQAHSAEINPHTPHPVIDLLPE
CCHHHHHEEHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
QKDIEDLGGTLRLGAYPCKLVDGTKAKEAYDGADVIEERHRHRYEFNNVYRDQMAEKGFV
CCCHHHCCCEEEECCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSGTSPDGRLVETIEVKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVDYRKG
EECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGITAASLGRLLKNRGLSVTIQKFDPYINVDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCCCCCEEE
VTQDGAETDLDLGHYERFIDINLNKYSNITTGKVYSSVIRKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHEEHHHHHH
EIKDQVFRAGEATGADVVITEIGGTVGDIESLPFLEAIRQIKSDVGRDSVMYVHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
IKAAGEMKTKPTQHSVKELRSLGIQPDAIVLRTEMAISKDMKEKIALFCDIDDKSVIEMR
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCEEEEEECCCCCEEEEC
DADTLYQVPIALQEEKLDQITCNHFGLDCPEADMEEWKGLVNKVRNLSKTTHIGLVGKYV
CCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEHHH
ELPDAYLSVVESLKHAGYDYDTDVKIHWINSELLSEEQIKEELSKVDGVLVPGGFGDRGI
HCCHHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCHHHHHHHHHHHCCEEECCCCCCCCC
DGKIEAIRYARENNIPFFGICLGMQLASVEFARNVVGLSQAHSAEINPHTPHPVIDLLPE
CCHHHHHEEHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHCCCC
QKDIEDLGGTLRLGAYPCKLVDGTKAKEAYDGADVIEERHRHRYEFNNVYRDQMAEKGFV
CCCHHHCCCEEEECCCCEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSGTSPDGRLVETIEVKDHPWFVACQFHPEFTSRPTRAQSLFKGFIGASVDYRKG
EECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376