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The map label for this gene is rho [H]

Identifier: 23100456

GI number: 23100456

Start: 3109026

End: 3110306

Strand: Reverse

Name: rho [H]

Synonym: OB3001

Alternate gene names: 23100456

Gene position: 3110306-3109026 (Counterclockwise)

Preceding gene: 23100457

Following gene: 23100455

Centisome position: 85.67

GC content: 37.24

Gene sequence:

>1281_bases
GTGGAAACATTAACAATCTCTAATTTAGAGACAATGACATTAAAGGAAATTTATGCGTTGGCGAAGGAATATAAAGTTTC
TTATTATGCTAAGCTGACAAAAAAAGAACTAATCTTTGCTATTTTAAAAGCCCAAGCGGAAAAAGATGGATTTCTATTTA
TGGATGGTATCCTTGAAATTATACCTTCGGAAGGTTTTGGTTTTCTACGTCCAATTAATTATTCTCCAAGTGCAGAAGAT
ATCTATATTTCAGCTTCCCAAATACGTCGATTTGATTTAAGAAACGGTGATAAAGTTTCTGGTAAGGTACGTCCTCCTAA
AGAAAATGAAAGATACTATGGATTACTTCATGTCGATGCAGTTAATGATGAAGATCCTGATTCTTCCAAAGAACGAGTTC
ATTTCCCTGCATTAACAGCCTTATATCCAAATCGTACGATACAACTCGAGACAAAAACACATCAATTATCTACTCGAATT
ATGGACTTGATGACTCCAGTTGGTTTTGGACAACGTGGATTAATTGTTGCTCCACCAAAAGCTGGTAAGACAATGTTAAT
TAAAGAAATTGCGAACAGCATCTCTAAAAATCATCCTCACGCTAAACTAATTATTTTATTAGTAGATGAGCGACCGGAAG
AAGTGACAGATATTGAACGCTCTGTGTCAGCGGATGTTGACGTTGTTAGCTCGACATTTGATGAAGTTCCGGAGAGTCAC
ATAAAGGTTTCGGAATTGGTATTAGAACGTGCAATGCGTTTAGTTGAACATAAACGTGATGTTATTGTACTTATGGATAG
TATTACTCGACTAGCAAGAGCATATAACCTAGTGATTCCTCCAAGTGGGCGAACACTTTCTGGCGGTATTGACCCAGCAG
CATTTCATCGTCCAAAACGTTTCTTTGGCGCTGCACGTAATATCGAAGAGGGTGGTAGTTTTACAATTCTGGCAACTGCT
CTCGTTGATACTGGATCTCGCATGGATGATGTTATCTATGAAGAATTTAAAGGTACAGGGAATATGGAGTTGCATCTTGA
TCGTCATCTTGCCGATCGACGTATATTCCCAGCAATAGATATTATGCGTTCGGGTACACGTAAAGAAGAGTTGTTAGTTG
GAAAAAGCCATCTCGATAAAATGTGGGCTATTCGTAAAACAATGCAGGATTCCCATGATTTCTTAGATCGTTTCTTAAAA
CGACTAAAACAATCGAAAAGTAATGAAGAATTCCTCCAATTAATGGAAGAAGAAATGAAAAGAAAAGGAATTTCTCGATA
G

Upstream 100 bases:

>100_bases
TCCAATTTAGATGGATAATCGATAAGGTATATGCGTTTTAGTGAAAGAAAATAAAGAAAAAATGTAATAAAACAAGAATC
TAGACAGGCGTGGTGATTTA

Downstream 100 bases:

>100_bases
TTTAGTCTGAAGTTAAATTTCTTTCTGGTTTCAAACAAATAACTAACCATGAATTTATTTCTATTAATACAGATGAGTAA
TATCCTCCTGTTGCATTTAG

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
METLTISNLETMTLKEIYALAKEYKVSYYAKLTKKELIFAILKAQAEKDGFLFMDGILEIIPSEGFGFLRPINYSPSAED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVDAVNDEDPDSSKERVHFPALTALYPNRTIQLETKTHQLSTRI
MDLMTPVGFGQRGLIVAPPKAGKTMLIKEIANSISKNHPHAKLIILLVDERPEEVTDIERSVSADVDVVSSTFDEVPESH
IKVSELVLERAMRLVEHKRDVIVLMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSFTILATA
LVDTGSRMDDVIYEEFKGTGNMELHLDRHLADRRIFPAIDIMRSGTRKEELLVGKSHLDKMWAIRKTMQDSHDFLDRFLK
RLKQSKSNEEFLQLMEEEMKRKGISR

Sequences:

>Translated_426_residues
METLTISNLETMTLKEIYALAKEYKVSYYAKLTKKELIFAILKAQAEKDGFLFMDGILEIIPSEGFGFLRPINYSPSAED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVDAVNDEDPDSSKERVHFPALTALYPNRTIQLETKTHQLSTRI
MDLMTPVGFGQRGLIVAPPKAGKTMLIKEIANSISKNHPHAKLIILLVDERPEEVTDIERSVSADVDVVSSTFDEVPESH
IKVSELVLERAMRLVEHKRDVIVLMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSFTILATA
LVDTGSRMDDVIYEEFKGTGNMELHLDRHLADRRIFPAIDIMRSGTRKEELLVGKSHLDKMWAIRKTMQDSHDFLDRFLK
RLKQSKSNEEFLQLMEEEMKRKGISR
>Mature_426_residues
METLTISNLETMTLKEIYALAKEYKVSYYAKLTKKELIFAILKAQAEKDGFLFMDGILEIIPSEGFGFLRPINYSPSAED
IYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVDAVNDEDPDSSKERVHFPALTALYPNRTIQLETKTHQLSTRI
MDLMTPVGFGQRGLIVAPPKAGKTMLIKEIANSISKNHPHAKLIILLVDERPEEVTDIERSVSADVDVVSSTFDEVPESH
IKVSELVLERAMRLVEHKRDVIVLMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKRFFGAARNIEEGGSFTILATA
LVDTGSRMDDVIYEEFKGTGNMELHLDRHLADRRIFPAIDIMRSGTRKEELLVGKSHLDKMWAIRKTMQDSHDFLDRFLK
RLKQSKSNEEFLQLMEEEMKRKGISR

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=423, Percent_Identity=53.4278959810875, Blast_Score=467, Evalue=1e-133,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48463; Mature: 48463

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLTISNLETMTLKEIYALAKEYKVSYYAKLTKKELIFAILKAQAEKDGFLFMDGILEI
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
IPSEGFGFLRPINYSPSAEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVDA
CCCCCCCCEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNDEDPDSSKERVHFPALTALYPNRTIQLETKTHQLSTRIMDLMTPVGFGQRGLIVAPPK
CCCCCCCCCCCEEECCCCEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCC
AGKTMLIKEIANSISKNHPHAKLIILLVDERPEEVTDIERSVSADVDVVSSTFDEVPESH
CCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHH
IKVSELVLERAMRLVEHKRDVIVLMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHCCCHH
FFGAARNIEEGGSFTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRHLADRRIFPAID
HHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCHHHH
IMRSGTRKEELLVGKSHLDKMWAIRKTMQDSHDFLDRFLKRLKQSKSNEEFLQLMEEEMK
HHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RKGISR
HCCCCC
>Mature Secondary Structure
METLTISNLETMTLKEIYALAKEYKVSYYAKLTKKELIFAILKAQAEKDGFLFMDGILEI
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHH
IPSEGFGFLRPINYSPSAEDIYISASQIRRFDLRNGDKVSGKVRPPKENERYYGLLHVDA
CCCCCCCCEECCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEE
VNDEDPDSSKERVHFPALTALYPNRTIQLETKTHQLSTRIMDLMTPVGFGQRGLIVAPPK
CCCCCCCCCCCEEECCCCEEECCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCC
AGKTMLIKEIANSISKNHPHAKLIILLVDERPEEVTDIERSVSADVDVVSSTFDEVPESH
CCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCHHH
IKVSELVLERAMRLVEHKRDVIVLMDSITRLARAYNLVIPPSGRTLSGGIDPAAFHRPKR
HHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEECCCCCEECCCCCHHHHCCCHH
FFGAARNIEEGGSFTILATALVDTGSRMDDVIYEEFKGTGNMELHLDRHLADRRIFPAID
HHHHHCCCCCCCCEEEEEEEHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCHHHH
IMRSGTRKEELLVGKSHLDKMWAIRKTMQDSHDFLDRFLKRLKQSKSNEEFLQLMEEEMK
HHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RKGISR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]