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The map label for this gene is ywkE [H]

Identifier: 23100452

GI number: 23100452

Start: 3105573

End: 3106436

Strand: Reverse

Name: ywkE [H]

Synonym: OB2997

Alternate gene names: 23100452

Gene position: 3106436-3105573 (Counterclockwise)

Preceding gene: 23100453

Following gene: 23100451

Centisome position: 85.56

GC content: 37.04

Gene sequence:

>864_bases
ATGAGCGAGAAATATTTTGAAGTCCTTCAACGGGCTTCTCTTTTTTTAGAAAAGGCTGGGCGCGAACCAAAAGTTGCAGA
GATACTCCTATTACATCATTTACAAATATCAAAAGAGCAATTGTTAATGAAATGGAGAGATTCCATTCAAGAAAGTAGCG
TTGAAGCAATTGAGAAAGATATTCTTACCCATGTAGAGACTGGTGTTCCAGTTCAACATTTAACCAAAGAAGAACATTTT
TATGGTCGCGTATTCGAGGTTAATCAGCATGTATTAATTCCTCGTCCGGAAACAGAAGAGTTAGTCCAACAAGTAATCCA
ACAAGCCAAGACAATGGAGCAACCAATAACCATTGTTGATGTTGGAACGGGTAGCGGAGTTATTGCAATAACATTAGCAC
TTGAGCTTCCAGGCTCACGCGTTTTTGCGACGGATATTTCAGAAAAAGCATTAGCTGTTGCCAAAAGAAATGCTCAAGCG
ATGAAGGCGGACGTAACTTTCCTGGAAGGGAACTTTCTAGAGCCATTTTTAGATATAAATCAAACAGCAGATATCATTGT
TTCCAATCCACCCTATATACCTTGGTCACAATCTGAAGAGTTACAAGATACCGTTAAAAATTTTGACCCTGAATTAGCAT
TATTTGCAGAGAATAATGGCTTAGCAGCCTATCATGCTATTTTAAGACAAATGAAGACGATGAAATTATCTGATCAATCT
ATCTTAGCTTTTGAAATCGGATTTGATCAGTCAGAAGCAATTACCAATATGATTAGACAGAACCTACCTTCAAGAGAAGT
ATTTACGGTAAAAGATATAAATGACAAAGACCGAATTCTGTTAGCTTATGTTATAAGAGGTTAA

Upstream 100 bases:

>100_bases
AAATTGGATCAAATTATGCAAGGGAAGTTAGATGAATTTATTGATGCTTTAGTAATGGAAGAACAAACGAATAAATTAGA
GCAGATAGGTGAGTAATTAA

Downstream 100 bases:

>100_bases
AAATAAAATTACAAAAAGAGAAATCAAAAGTTTGGTAAAATGTCGGATGATAACTAGAATAAGCGTAAGAAATAAAAGGA
TGCTCACCAGTTGGATAAGT

Product: protoporphyrinogen oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSEKYFEVLQRASLFLEKAGREPKVAEILLLHHLQISKEQLLMKWRDSIQESSVEAIEKDILTHVETGVPVQHLTKEEHF
YGRVFEVNQHVLIPRPETEELVQQVIQQAKTMEQPITIVDVGTGSGVIAITLALELPGSRVFATDISEKALAVAKRNAQA
MKADVTFLEGNFLEPFLDINQTADIIVSNPPYIPWSQSEELQDTVKNFDPELALFAENNGLAAYHAILRQMKTMKLSDQS
ILAFEIGFDQSEAITNMIRQNLPSREVFTVKDINDKDRILLAYVIRG

Sequences:

>Translated_287_residues
MSEKYFEVLQRASLFLEKAGREPKVAEILLLHHLQISKEQLLMKWRDSIQESSVEAIEKDILTHVETGVPVQHLTKEEHF
YGRVFEVNQHVLIPRPETEELVQQVIQQAKTMEQPITIVDVGTGSGVIAITLALELPGSRVFATDISEKALAVAKRNAQA
MKADVTFLEGNFLEPFLDINQTADIIVSNPPYIPWSQSEELQDTVKNFDPELALFAENNGLAAYHAILRQMKTMKLSDQS
ILAFEIGFDQSEAITNMIRQNLPSREVFTVKDINDKDRILLAYVIRG
>Mature_286_residues
SEKYFEVLQRASLFLEKAGREPKVAEILLLHHLQISKEQLLMKWRDSIQESSVEAIEKDILTHVETGVPVQHLTKEEHFY
GRVFEVNQHVLIPRPETEELVQQVIQQAKTMEQPITIVDVGTGSGVIAITLALELPGSRVFATDISEKALAVAKRNAQAM
KADVTFLEGNFLEPFLDINQTADIIVSNPPYIPWSQSEELQDTVKNFDPELALFAENNGLAAYHAILRQMKTMKLSDQSI
LAFEIGFDQSEAITNMIRQNLPSREVFTVKDINDKDRILLAYVIRG

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=230, Percent_Identity=29.5652173913043, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1787463, Length=261, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87082085, Length=192, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24582226, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32469; Mature: 32337

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKYFEVLQRASLFLEKAGREPKVAEILLLHHLQISKEQLLMKWRDSIQESSVEAIEKD
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILTHVETGVPVQHLTKEEHFYGRVFEVNQHVLIPRPETEELVQQVIQQAKTMEQPITIVD
HHHHHHCCCCHHHHCCHHHHHEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
VGTGSGVIAITLALELPGSRVFATDISEKALAVAKRNAQAMKADVTFLEGNFLEPFLDIN
ECCCCCEEEEEEEEECCCCEEEECCCHHHHHHHHHHCHHHHHHEEEEECCCCCCHHHCCC
QTADIIVSNPPYIPWSQSEELQDTVKNFDPELALFAENNGLAAYHAILRQMKTMKLSDQS
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCE
ILAFEIGFDQSEAITNMIRQNLPSREVFTVKDINDKDRILLAYVIRG
EEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECC
>Mature Secondary Structure 
SEKYFEVLQRASLFLEKAGREPKVAEILLLHHLQISKEQLLMKWRDSIQESSVEAIEKD
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILTHVETGVPVQHLTKEEHFYGRVFEVNQHVLIPRPETEELVQQVIQQAKTMEQPITIVD
HHHHHHCCCCHHHHCCHHHHHEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCEEEEE
VGTGSGVIAITLALELPGSRVFATDISEKALAVAKRNAQAMKADVTFLEGNFLEPFLDIN
ECCCCCEEEEEEEEECCCCEEEECCCHHHHHHHHHHCHHHHHHEEEEECCCCCCHHHCCC
QTADIIVSNPPYIPWSQSEELQDTVKNFDPELALFAENNGLAAYHAILRQMKTMKLSDQS
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCE
ILAFEIGFDQSEAITNMIRQNLPSREVFTVKDINDKDRILLAYVIRG
EEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]