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The map label for this gene is prfA

Identifier: 23100453

GI number: 23100453

Start: 3106441

End: 3107511

Strand: Reverse

Name: prfA

Synonym: OB2998

Alternate gene names: 23100453

Gene position: 3107511-3106441 (Counterclockwise)

Preceding gene: 23100454

Following gene: 23100452

Centisome position: 85.59

GC content: 37.54

Gene sequence:

>1071_bases
ATGTTAGATCGTTTGCAATCGTTAGAGGACAGATATAATAAATTAAATGAAATGCTTAGTGACCCAGAGGTTATTAACGA
TTCTAAAAAACTTCGTGAGTACTCGAAAGAACAATCTGGCTTAGAAGATGTCGTTCAAGCTTATCGTGAGTATAAAGAAG
TAACAGAACAATTAAATGATGCAAAAGAAATGATGGAAGATAAATTAGACGATGAAATGTATGAAATGGTGAAAGCAGAA
ATCGCTGAACTAAATGGACGAAAAGAAGAGCTAGAGTCCAGTATGAAGATTCTTCTTTTACCTAAAGATCCTAATGATGA
TAAGAACGTATTTATGGAAATACGTGGTGCAGCTGGAGGAGATGAAGCTGCTTTATTTGCGGGTGATTTATACCGCATGT
ATTCTCGATATGCAGAACACCAGGGGTGGAAAATTGACGTCATGGAAGCAAGTTCCACAGGTGTAGGCGGCTATAAAGAG
GTCATCTTTATGATTAATGGATCCGATGTTTATTCGAAATTAAAATATGAAAATGGGGCTCATCGAGTTCAACGGGTACC
AGAAACAGAATCTGGAGGACGTATACATACTTCAACAGCAACTGTAGCTGTGCTTCCAGAAGCAGAAGAGGTAGAAGTAG
AAGTTCATGATAAGGATATCCGTGTAGATACATTTGCATCCAGTGGTCCAGGAGGACAAAGTGTAAATACAACGATGTCT
GCTGTTAGACTTACGCATGTTCCAACTGGAATTGTAGTATCCATCCAAGATGAGAAATCCCAAATTAAAAATAAAGAAAA
AGCAATGAAAGTATTACGTGCACGTATCTATGATAAATTTCAACAAGAAGCACAAGCGGAGTATGATGAAAATCGTAAAT
CTGCGGTTGGTTCAGGCGACCGTTCTGAACGAATTCGTACGTATAATTTCCCGCAAAATCGTGTAACGGATCACCGAATT
GGCTTAACGATTCAAAAATTGGATCAAATTATGCAAGGGAAGTTAGATGAATTTATTGATGCTTTAGTAATGGAAGAACA
AACGAATAAATTAGAGCAGATAGGTGAGTAA

Upstream 100 bases:

>100_bases
ACTATCCTTTTTACATACTGGTTTATCCTAGGTGGTCAATTGACCCAAGGACTCCCATATAATATAATAATACTGTTAAT
CAGAAAGAGGTGTATGTCCG

Downstream 100 bases:

>100_bases
TTAAATGAGCGAGAAATATTTTGAAGTCCTTCAACGGGCTTCTCTTTTTTTAGAAAAGGCTGGGCGCGAACCAAAAGTTG
CAGAGATACTCCTATTACAT

Product: peptide chain release factor RF-1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MLDRLQSLEDRYNKLNEMLSDPEVINDSKKLREYSKEQSGLEDVVQAYREYKEVTEQLNDAKEMMEDKLDDEMYEMVKAE
IAELNGRKEELESSMKILLLPKDPNDDKNVFMEIRGAAGGDEAALFAGDLYRMYSRYAEHQGWKIDVMEASSTGVGGYKE
VIFMINGSDVYSKLKYENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEVEVHDKDIRVDTFASSGPGGQSVNTTMS
AVRLTHVPTGIVVSIQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDENRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQIMQGKLDEFIDALVMEEQTNKLEQIGE

Sequences:

>Translated_356_residues
MLDRLQSLEDRYNKLNEMLSDPEVINDSKKLREYSKEQSGLEDVVQAYREYKEVTEQLNDAKEMMEDKLDDEMYEMVKAE
IAELNGRKEELESSMKILLLPKDPNDDKNVFMEIRGAAGGDEAALFAGDLYRMYSRYAEHQGWKIDVMEASSTGVGGYKE
VIFMINGSDVYSKLKYENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEVEVHDKDIRVDTFASSGPGGQSVNTTMS
AVRLTHVPTGIVVSIQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDENRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQIMQGKLDEFIDALVMEEQTNKLEQIGE
>Mature_356_residues
MLDRLQSLEDRYNKLNEMLSDPEVINDSKKLREYSKEQSGLEDVVQAYREYKEVTEQLNDAKEMMEDKLDDEMYEMVKAE
IAELNGRKEELESSMKILLLPKDPNDDKNVFMEIRGAAGGDEAALFAGDLYRMYSRYAEHQGWKIDVMEASSTGVGGYKE
VIFMINGSDVYSKLKYENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEVEVHDKDIRVDTFASSGPGGQSVNTTMS
AVRLTHVPTGIVVSIQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDENRKSAVGSGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQIMQGKLDEFIDALVMEEQTNKLEQIGE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=329, Percent_Identity=41.0334346504559, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=35.7348703170029, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI166795305, Length=234, Percent_Identity=36.7521367521368, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=49.859943977591, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=35.5223880597015, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=313, Percent_Identity=36.1022364217252, Blast_Score=183, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=340, Percent_Identity=41.4705882352941, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19921226, Length=320, Percent_Identity=39.375, Blast_Score=241, Evalue=8e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_OCEIH (Q8EM60)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693920.1
- ProteinModelPortal:   Q8EM60
- SMR:   Q8EM60
- GeneID:   1017913
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2998
- NMPDR:   fig|221109.1.peg.3000
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- BioCyc:   OIHE221109:OB2998-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40498; Mature: 40498

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLQSLEDRYNKLNEMLSDPEVINDSKKLREYSKEQSGLEDVVQAYREYKEVTEQLND
CCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AKEMMEDKLDDEMYEMVKAEIAELNGRKEELESSMKILLLPKDPNDDKNVFMEIRGAAGG
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEHQGWKIDVMEASSTGVGGYKEVIFMINGSDVYSKLKYENGA
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHCCCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEVEVHDKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHVPTGIVVSIQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDENRKSAVGSGD
HHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQIMQGKLDEFIDALVMEEQTNKLEQIGE
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDRLQSLEDRYNKLNEMLSDPEVINDSKKLREYSKEQSGLEDVVQAYREYKEVTEQLND
CCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AKEMMEDKLDDEMYEMVKAEIAELNGRKEELESSMKILLLPKDPNDDKNVFMEIRGAAGG
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEHQGWKIDVMEASSTGVGGYKEVIFMINGSDVYSKLKYENGA
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHHCCCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEVEVHDKDIRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCCEEEEEECCCEEEEECCCCCCCCCCHHHHHH
AVRLTHVPTGIVVSIQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDENRKSAVGSGD
HHHHEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQIMQGKLDEFIDALVMEEQTNKLEQIGE
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376