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The map label for this gene is dppE [H]

Identifier: 23100422

GI number: 23100422

Start: 3076034

End: 3077695

Strand: Reverse

Name: dppE [H]

Synonym: OB2967

Alternate gene names: 23100422

Gene position: 3077695-3076034 (Counterclockwise)

Preceding gene: 23100423

Following gene: 23100421

Centisome position: 84.77

GC content: 38.87

Gene sequence:

>1662_bases
ATGAAGAACAAGAAATTACTGTTGTTTTTGTTGGCTTTATTAACTGCATTTGTATTAGCAGCTTGTACTGCAAATGAAGA
TGCGGGAGAGCCAGATGATGCTGATGCAGGGGAAACGGAAGAAGGGGAAGGTCAAGAAGGAGAAGCTGCAGGTGAAAAAG
TTCTTCGTGTAAATAATGGAGTAGAACCAACATCCCTTGATCCATCGATAGGGTTTGATCAGGTTTCTTGGGATCCATTA
AACAACTTAATGGAGGGTCTGACTAGATTAGATCAAGAACATGTTGCCGGTCCGGGTGTGGCGGAGAGTTGGGACATATC
AGAAGACGGATTAACGTACACATTCCATTTACGTGAAGATGCAAACTGGTCGAATGGAGATCCGGTAGTTGCAGAAGACT
TTGTATATGCATGGAAGTATATGTTGGATCCAGCGAATGCTTCATCAGCTGCCTTTTTAGCATATTTTATTGAAGGTGCA
GAAGCATTTAATACTGGGGAAGGTTCAGCAGATGATGTCAATATTACTGCTGTAGATGAAAAGACATTAGAAGTAGTATT
GGAAGCACCAACTGGATTTTTCCTAGATGTGTTAACCAATCCGGCATTCTTCCCAGTAAATCATAAAGTGGCAGAAGAAA
ATCCTGATTGGCATGCAGAAGCTGATTCATTCGTAGCAAACGGCCCATTTCAATTAGAATCTTGGGAGCATGATAGTGAA
ATGGTATTTGCCAAAAATCCAGAGTATTGGGATGCTGATGCTGTGAATTTAGATAAGATCCATTTTGCAATGGTAAATGA
TACGAATACACAATACCAAATGTTTGAAACTGGAGAGCTCGATACAGCAACAATCCCTCCAGAAATGTCAGATGAATTAA
TTGATGGTGATAATGTATTTATCGGCGATTATGGTGGATTAGAATTCTTCCGTTTTAACACGACAGAAGAACCATTCCAA
AATAAAAATATTCGTAAGGCATTTGCCTATGCCATTAATCGTGATGATATTGCTACCTATGTCGTGAAAAATGGTGTAGA
GCCGGCATATGGATTTGTATCGCCTGGATTTACTACGCCAGATGGTGGGGATTTCCGTGATGAAAATGGAAATTTAGTAG
AGTTTGATCCAGAGACTGCAAAAGAACTATTAGAACAAGGGATGGAAGAAGAAGGCTATAGTGAACTTCCAAGCATTGTT
TTATCTTATAACACAAGCGACACGAATAAAGCTGTTGCGGAATCCATTCAAAATATGTTGAATGAAAATCTTGGTGTGGA
AGTAACATTAGAAAACCAAGAATGGAATGTATTTGCTGAAGCACAGCAAAACTTAGAATTGCAATTATCACGTAGTTCAT
TTATTAATGACTACAGTGATCCGGTAAACTTCTTAGAGAGCTTTATTACGGACTCTTACATGAACCGTACTGGTTGGTCG
AATGAAGAATACGATGCATTAATTGAAAAAGGAAAAACAGAAACAGATGAAGAACAACGCTACGAATATCTATATGAAGC
GGAAAGATTGCTTGCAGAAGAAATGGTTGCAATGCCAATTCGTTACTATAATACGGTAGTTCTAGAAGCGGATCATGTGG
AAGGAATTCTACGTCATCCAGTTGGTTATTTTGATCTGAAATATGCTGACGTAACAGAGTAA

Upstream 100 bases:

>100_bases
TAACATCATCACATAATGTGGATTGTTGGCTTCAAGATGAACGGGCACAACAGCTATTAGCTGAAAGTTCTTTTCAATAT
TAAAAAAGGGGGAAAATGGT

Downstream 100 bases:

>100_bases
ATCATACTTCTAAAACATAACAAAAGATATTTTATTTCGTAAGTTGAAAGATAGCTAGAATAATAAATATACAGAGAATC
TTGTGGAAGTGAGGCATAGG

Product: dipeptide ABC transporter dipeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKNKKLLLFLLALLTAFVLAACTANEDAGEPDDADAGETEEGEGQEGEAAGEKVLRVNNGVEPTSLDPSIGFDQVSWDPL
NNLMEGLTRLDQEHVAGPGVAESWDISEDGLTYTFHLREDANWSNGDPVVAEDFVYAWKYMLDPANASSAAFLAYFIEGA
EAFNTGEGSADDVNITAVDEKTLEVVLEAPTGFFLDVLTNPAFFPVNHKVAEENPDWHAEADSFVANGPFQLESWEHDSE
MVFAKNPEYWDADAVNLDKIHFAMVNDTNTQYQMFETGELDTATIPPEMSDELIDGDNVFIGDYGGLEFFRFNTTEEPFQ
NKNIRKAFAYAINRDDIATYVVKNGVEPAYGFVSPGFTTPDGGDFRDENGNLVEFDPETAKELLEQGMEEEGYSELPSIV
LSYNTSDTNKAVAESIQNMLNENLGVEVTLENQEWNVFAEAQQNLELQLSRSSFINDYSDPVNFLESFITDSYMNRTGWS
NEEYDALIEKGKTETDEEQRYEYLYEAERLLAEEMVAMPIRYYNTVVLEADHVEGILRHPVGYFDLKYADVTE

Sequences:

>Translated_553_residues
MKNKKLLLFLLALLTAFVLAACTANEDAGEPDDADAGETEEGEGQEGEAAGEKVLRVNNGVEPTSLDPSIGFDQVSWDPL
NNLMEGLTRLDQEHVAGPGVAESWDISEDGLTYTFHLREDANWSNGDPVVAEDFVYAWKYMLDPANASSAAFLAYFIEGA
EAFNTGEGSADDVNITAVDEKTLEVVLEAPTGFFLDVLTNPAFFPVNHKVAEENPDWHAEADSFVANGPFQLESWEHDSE
MVFAKNPEYWDADAVNLDKIHFAMVNDTNTQYQMFETGELDTATIPPEMSDELIDGDNVFIGDYGGLEFFRFNTTEEPFQ
NKNIRKAFAYAINRDDIATYVVKNGVEPAYGFVSPGFTTPDGGDFRDENGNLVEFDPETAKELLEQGMEEEGYSELPSIV
LSYNTSDTNKAVAESIQNMLNENLGVEVTLENQEWNVFAEAQQNLELQLSRSSFINDYSDPVNFLESFITDSYMNRTGWS
NEEYDALIEKGKTETDEEQRYEYLYEAERLLAEEMVAMPIRYYNTVVLEADHVEGILRHPVGYFDLKYADVTE
>Mature_553_residues
MKNKKLLLFLLALLTAFVLAACTANEDAGEPDDADAGETEEGEGQEGEAAGEKVLRVNNGVEPTSLDPSIGFDQVSWDPL
NNLMEGLTRLDQEHVAGPGVAESWDISEDGLTYTFHLREDANWSNGDPVVAEDFVYAWKYMLDPANASSAAFLAYFIEGA
EAFNTGEGSADDVNITAVDEKTLEVVLEAPTGFFLDVLTNPAFFPVNHKVAEENPDWHAEADSFVANGPFQLESWEHDSE
MVFAKNPEYWDADAVNLDKIHFAMVNDTNTQYQMFETGELDTATIPPEMSDELIDGDNVFIGDYGGLEFFRFNTTEEPFQ
NKNIRKAFAYAINRDDIATYVVKNGVEPAYGFVSPGFTTPDGGDFRDENGNLVEFDPETAKELLEQGMEEEGYSELPSIV
LSYNTSDTNKAVAESIQNMLNENLGVEVTLENQEWNVFAEAQQNLELQLSRSSFINDYSDPVNFLESFITDSYMNRTGWS
NEEYDALIEKGKTETDEEQRYEYLYEAERLLAEEMVAMPIRYYNTVVLEADHVEGILRHPVGYFDLKYADVTE

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=541, Percent_Identity=31.4232902033272, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789397, Length=501, Percent_Identity=30.7385229540918, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI87081878, Length=486, Percent_Identity=30.2469135802469, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI1789966, Length=471, Percent_Identity=23.5668789808917, Blast_Score=125, Evalue=6e-30,
Organism=Escherichia coli, GI1787052, Length=501, Percent_Identity=23.9520958083832, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787762, Length=516, Percent_Identity=21.8992248062016, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1789887, Length=479, Percent_Identity=24.0083507306889, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787551, Length=478, Percent_Identity=22.3849372384937, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61912; Mature: 61912

Theoretical pI: Translated: 3.73; Mature: 3.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKLLLFLLALLTAFVLAACTANEDAGEPDDADAGETEEGEGQEGEAAGEKVLRVNNG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCC
VEPTSLDPSIGFDQVSWDPLNNLMEGLTRLDQEHVAGPGVAESWDISEDGLTYTFHLRED
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
ANWSNGDPVVAEDFVYAWKYMLDPANASSAAFLAYFIEGAEAFNTGEGSADDVNITAVDE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KTLEVVLEAPTGFFLDVLTNPAFFPVNHKVAEENPDWHAEADSFVANGPFQLESWEHDSE
CEEEEEEECCCCEEHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCC
MVFAKNPEYWDADAVNLDKIHFAMVNDTNTQYQMFETGELDTATIPPEMSDELIDGDNVF
EEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCEE
IGDYGGLEFFRFNTTEEPFQNKNIRKAFAYAINRDDIATYVVKNGVEPAYGFVSPGFTTP
EECCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DGGDFRDENGNLVEFDPETAKELLEQGMEEEGYSELPSIVLSYNTSDTNKAVAESIQNML
CCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHH
NENLGVEVTLENQEWNVFAEAQQNLELQLSRSSFINDYSDPVNFLESFITDSYMNRTGWS
HCCCCEEEEEECCCCEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NEEYDALIEKGKTETDEEQRYEYLYEAERLLAEEMVAMPIRYYNTVVLEADHVEGILRHP
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHCC
VGYFDLKYADVTE
CCCEEEEECCCCC
>Mature Secondary Structure
MKNKKLLLFLLALLTAFVLAACTANEDAGEPDDADAGETEEGEGQEGEAAGEKVLRVNNG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEEECCC
VEPTSLDPSIGFDQVSWDPLNNLMEGLTRLDQEHVAGPGVAESWDISEDGLTYTFHLRED
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECC
ANWSNGDPVVAEDFVYAWKYMLDPANASSAAFLAYFIEGAEAFNTGEGSADDVNITAVDE
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
KTLEVVLEAPTGFFLDVLTNPAFFPVNHKVAEENPDWHAEADSFVANGPFQLESWEHDSE
CEEEEEEECCCCEEHHHHCCCCEECCCCCCCCCCCCCCCCCCCEEECCCEEECCCCCCCC
MVFAKNPEYWDADAVNLDKIHFAMVNDTNTQYQMFETGELDTATIPPEMSDELIDGDNVF
EEEECCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCHHCCCCCEE
IGDYGGLEFFRFNTTEEPFQNKNIRKAFAYAINRDDIATYVVKNGVEPAYGFVSPGFTTP
EECCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
DGGDFRDENGNLVEFDPETAKELLEQGMEEEGYSELPSIVLSYNTSDTNKAVAESIQNML
CCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHH
NENLGVEVTLENQEWNVFAEAQQNLELQLSRSSFINDYSDPVNFLESFITDSYMNRTGWS
HCCCCEEEEEECCCCEEEEECCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCCC
NEEYDALIEKGKTETDEEQRYEYLYEAERLLAEEMVAMPIRYYNTVVLEADHVEGILRHP
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHHHHHHHCC
VGYFDLKYADVTE
CCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]