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The map label for this gene is ykfA [H]

Identifier: 23100421

GI number: 23100421

Start: 3074896

End: 3075813

Strand: Reverse

Name: ykfA [H]

Synonym: OB2966

Alternate gene names: 23100421

Gene position: 3075813-3074896 (Counterclockwise)

Preceding gene: 23100422

Following gene: 23100420

Centisome position: 84.72

GC content: 37.36

Gene sequence:

>918_bases
ATGATACTTCCTAAACGTCTTAAAGAAGGGGATAAAGTTGGGATAGTTGCCCCAGCAGGACCACCTAACCGAGAAAAGGT
AAATAAATCTGTAACCTTTTTTGAACAGTTAGGCCTTTCCGTGCAGTTTGGTAAGTACCTTTATGACGAAAGAGGTTATT
TGTCGGCTGGTGATGAACAACGATTGGAAGATTTTCATGAGATGATTGCAGATAGTAGTATTAAAGCTATTTTTTTCGCG
AGAGGTGGATATGGAACCGCTCGTATCGTTGATCAATTAGATATGAATTTAATTGGAAATAACCCCAAAATTATATGGGG
TTATAGTGATATAACGTATTTACATACGGCTATACGCCAAGCTACTGGATTGGTCTCCTTTCATGGCCCGATGGCCGCAT
CTGATATTGTGAAAGCCGATTTTGATCGTTTCTCAGGTCAATTATTTAAACAATTATTTAAACCTACTCAATTAATTTAC
TCAGAAAAAGTCTCTCCGCTTTACGTATATACAAAGGGAATTGCAAAAGGAGAAGTTGTTGGGGGGAATCTCTCGTTATT
AGTGAGTTCCATGGGAACATCTTATGAGATTGATACAAAAAATAAACTATTATTAATAGAAGATATAGGAGAAGAACCTT
ATCGTGTAGATAGTATGCTTAATCAACTACGACTTGCCGGTAAGTTAGAAGATGCAGCTGGAATTATTGTAGGAGACTTT
TCTGAAGCGGAACCTACTGTCCAACCAAGTCTTTCTTTAACAGATGTGTTTGATGATTATTTTCGACCACTCGGAAAACC
TGTTATCGGAGGGTTTAAAATTGGACATTGTTTTCCTCATTTTTCAATGCCATTAGGAGTTGTGGCGGAATTATCTTCAG
AAAGCAAATCGTTGACAATTGATCCAGGTGTGAAATAA

Upstream 100 bases:

>100_bases
ACAGAGGCTCACCAGCCACTTCTATATATGTGCAGGATTTTCAAAAAAATTATTCGCATGTGCATTTATTAAATGGAATA
CATAAGAAAGAGGGTTTTTC

Downstream 100 bases:

>100_bases
AGGGAGAGAGGACATGAAGATAACAAGTGTAGAAACCTTTATAGGATCTGTGCCATTACACACTCCATTTAAAACAGCAT
TACGTACCGTAACAGTAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MILPKRLKEGDKVGIVAPAGPPNREKVNKSVTFFEQLGLSVQFGKYLYDERGYLSAGDEQRLEDFHEMIADSSIKAIFFA
RGGYGTARIVDQLDMNLIGNNPKIIWGYSDITYLHTAIRQATGLVSFHGPMAASDIVKADFDRFSGQLFKQLFKPTQLIY
SEKVSPLYVYTKGIAKGEVVGGNLSLLVSSMGTSYEIDTKNKLLLIEDIGEEPYRVDSMLNQLRLAGKLEDAAGIIVGDF
SEAEPTVQPSLSLTDVFDDYFRPLGKPVIGGFKIGHCFPHFSMPLGVVAELSSESKSLTIDPGVK

Sequences:

>Translated_305_residues
MILPKRLKEGDKVGIVAPAGPPNREKVNKSVTFFEQLGLSVQFGKYLYDERGYLSAGDEQRLEDFHEMIADSSIKAIFFA
RGGYGTARIVDQLDMNLIGNNPKIIWGYSDITYLHTAIRQATGLVSFHGPMAASDIVKADFDRFSGQLFKQLFKPTQLIY
SEKVSPLYVYTKGIAKGEVVGGNLSLLVSSMGTSYEIDTKNKLLLIEDIGEEPYRVDSMLNQLRLAGKLEDAAGIIVGDF
SEAEPTVQPSLSLTDVFDDYFRPLGKPVIGGFKIGHCFPHFSMPLGVVAELSSESKSLTIDPGVK
>Mature_305_residues
MILPKRLKEGDKVGIVAPAGPPNREKVNKSVTFFEQLGLSVQFGKYLYDERGYLSAGDEQRLEDFHEMIADSSIKAIFFA
RGGYGTARIVDQLDMNLIGNNPKIIWGYSDITYLHTAIRQATGLVSFHGPMAASDIVKADFDRFSGQLFKQLFKPTQLIY
SEKVSPLYVYTKGIAKGEVVGGNLSLLVSSMGTSYEIDTKNKLLLIEDIGEEPYRVDSMLNQLRLAGKLEDAAGIIVGDF
SEAEPTVQPSLSLTDVFDDYFRPLGKPVIGGFKIGHCFPHFSMPLGVVAELSSESKSLTIDPGVK

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=256, Percent_Identity=31.640625, Blast_Score=108, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33529; Mature: 33529

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPKRLKEGDKVGIVAPAGPPNREKVNKSVTFFEQLGLSVQFGKYLYDERGYLSAGDEQ
CCCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECHHHHCCCCCCCCCCHH
RLEDFHEMIADSSIKAIFFARGGYGTARIVDQLDMNLIGNNPKIIWGYSDITYLHTAIRQ
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHH
ATGLVSFHGPMAASDIVKADFDRFSGQLFKQLFKPTQLIYSEKVSPLYVYTKGIAKGEVV
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
GGNLSLLVSSMGTSYEIDTKNKLLLIEDIGEEPYRVDSMLNQLRLAGKLEDAAGIIVGDF
CCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
SEAEPTVQPSLSLTDVFDDYFRPLGKPVIGGFKIGHCFPHFSMPLGVVAELSSESKSLTI
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCEEEE
DPGVK
CCCCC
>Mature Secondary Structure
MILPKRLKEGDKVGIVAPAGPPNREKVNKSVTFFEQLGLSVQFGKYLYDERGYLSAGDEQ
CCCCCHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEECHHHHCCCCCCCCCCHH
RLEDFHEMIADSSIKAIFFARGGYGTARIVDQLDMNLIGNNPKIIWGYSDITYLHTAIRQ
HHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHH
ATGLVSFHGPMAASDIVKADFDRFSGQLFKQLFKPTQLIYSEKVSPLYVYTKGIAKGEVV
HHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
GGNLSLLVSSMGTSYEIDTKNKLLLIEDIGEEPYRVDSMLNQLRLAGKLEDAAGIIVGDF
CCHHHHHHHHCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECC
SEAEPTVQPSLSLTDVFDDYFRPLGKPVIGGFKIGHCFPHFSMPLGVVAELSSESKSLTI
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCCEEEE
DPGVK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]