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The map label for this gene is ykfB [H]

Identifier: 23100420

GI number: 23100420

Start: 3073776

End: 3074882

Strand: Reverse

Name: ykfB [H]

Synonym: OB2965

Alternate gene names: 23100420

Gene position: 3074882-3073776 (Counterclockwise)

Preceding gene: 23100421

Following gene: 23100419

Centisome position: 84.7

GC content: 37.67

Gene sequence:

>1107_bases
ATGAAGATAACAAGTGTAGAAACCTTTATAGGATCTGTGCCATTACACACTCCATTTAAAACAGCATTACGTACCGTAAC
AGTAGCTGAATCCATTATCGTCAAAATAACAAGCGAAGATGGTTATGTAGGGTGGGGAGAAGCTCCTCCTACCCATGTTA
TAACAGGAGAGAGTCTCGCAAGTATTGAATACGCTATTCATCATGTGATCAAACCATTTTTAATTGGAAATTCATTGTCG
ACTTATGAACGTATTTTTGAACAACTGCAAGCATGTATGATTGGTAATACGAGTGCCAAAGCAGCTGTTGATATGGCGAT
TTATGACTTATTAGCACAATATGCAAAACTACCACTTTATCAGTATTTAGGAGGATACCATTCGGAAATTGAAACGGATT
ATACCGTCAGTGTAAATACTGTGGAAGAAATGGTAAGAGATGCGAACCAATATAAATTAAATGGATTCTCGATATTGAAG
GTAAAGGTTGGCAAAGATCAAATTGAAACAGATATGGAACGAATCAAAGCGATTCGTCAATCAGTAGGAAATGAAGTTCG
TATTCGACTCGATGCGAACCAGGGTTGGAACCCCAAAGAAGCTGTTAAGGTAATTCAGAAAATGGAAGATCTGCAACTAA
ATATTGAGCTTGTTGAACAACCAGTTATAGCCAAAGATTTAGAAGGATTAAAGTTTGTAACCGATCGAACAGAAACTCCA
ATCATGGCAGATGAAGCGGTATTCTCTATCCACGATGCAAGACAAGTTCTAGAAAATAAGGCAGCTGATCTAATTAACAT
CAAATTAATGAAATCAGGTGGAATCCATCAAGCTAGAAAAATAGCAACTTTAGCGCAAAGCTATGGAATAGAATGTATGG
TTGGTAGCATGATAGAAACGAAACTAGGAATTAGTGCGGCTGCTCATTTTGCTGCAAGTCATCCAGTTGTCACAAGGTTT
GATTTCGATGCCCCGCTAATGCTATCAGGAGATTTGGTCCATGGTGGAGTAATTTATAAAGGAAGTCATCTATCTTTTAG
TGAGGATTATGGATTAGGAATCTGCGGAATTCGCTCTGAGTTTTTGAGACAAAACACAATGAAATAA

Upstream 100 bases:

>100_bases
TTTCCTCATTTTTCAATGCCATTAGGAGTTGTGGCGGAATTATCTTCAGAAAGCAAATCGTTGACAATTGATCCAGGTGT
GAAATAAAGGGAGAGAGGAC

Downstream 100 bases:

>100_bases
TTTCAGAATGGGGGAGCTGAAATATGAAACAAACGTTTGATCAATTATCCGAAGATTTTTGGGTGACCGATGTACAAGTG
GCAACAGTATGGACGAATCC

Product: muconate cycloisomerase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKITSVETFIGSVPLHTPFKTALRTVTVAESIIVKITSEDGYVGWGEAPPTHVITGESLASIEYAIHHVIKPFLIGNSLS
TYERIFEQLQACMIGNTSAKAAVDMAIYDLLAQYAKLPLYQYLGGYHSEIETDYTVSVNTVEEMVRDANQYKLNGFSILK
VKVGKDQIETDMERIKAIRQSVGNEVRIRLDANQGWNPKEAVKVIQKMEDLQLNIELVEQPVIAKDLEGLKFVTDRTETP
IMADEAVFSIHDARQVLENKAADLINIKLMKSGGIHQARKIATLAQSYGIECMVGSMIETKLGISAAAHFAASHPVVTRF
DFDAPLMLSGDLVHGGVIYKGSHLSFSEDYGLGICGIRSEFLRQNTMK

Sequences:

>Translated_368_residues
MKITSVETFIGSVPLHTPFKTALRTVTVAESIIVKITSEDGYVGWGEAPPTHVITGESLASIEYAIHHVIKPFLIGNSLS
TYERIFEQLQACMIGNTSAKAAVDMAIYDLLAQYAKLPLYQYLGGYHSEIETDYTVSVNTVEEMVRDANQYKLNGFSILK
VKVGKDQIETDMERIKAIRQSVGNEVRIRLDANQGWNPKEAVKVIQKMEDLQLNIELVEQPVIAKDLEGLKFVTDRTETP
IMADEAVFSIHDARQVLENKAADLINIKLMKSGGIHQARKIATLAQSYGIECMVGSMIETKLGISAAAHFAASHPVVTRF
DFDAPLMLSGDLVHGGVIYKGSHLSFSEDYGLGICGIRSEFLRQNTMK
>Mature_368_residues
MKITSVETFIGSVPLHTPFKTALRTVTVAESIIVKITSEDGYVGWGEAPPTHVITGESLASIEYAIHHVIKPFLIGNSLS
TYERIFEQLQACMIGNTSAKAAVDMAIYDLLAQYAKLPLYQYLGGYHSEIETDYTVSVNTVEEMVRDANQYKLNGFSILK
VKVGKDQIETDMERIKAIRQSVGNEVRIRLDANQGWNPKEAVKVIQKMEDLQLNIELVEQPVIAKDLEGLKFVTDRTETP
IMADEAVFSIHDARQVLENKAADLINIKLMKSGGIHQARKIATLAQSYGIECMVGSMIETKLGISAAAHFAASHPVVTRF
DFDAPLMLSGDLVHGGVIYKGSHLSFSEDYGLGICGIRSEFLRQNTMK

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has probably a role in the metabolism of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of the other Ala-X dipepti

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=332, Percent_Identity=30.421686746988, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787864, Length=353, Percent_Identity=26.628895184136, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI48994953, Length=286, Percent_Identity=25.8741258741259, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 40684; Mature: 40684

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSVETFIGSVPLHTPFKTALRTVTVAESIIVKITSEDGYVGWGEAPPTHVITGESLA
CCCCCHHHHHCCCCCCCCHHHHHHHHHHEEEEEEEEECCCCEEECCCCCCCEEECCCHHH
SIEYAIHHVIKPFLIGNSLSTYERIFEQLQACMIGNTSAKAAVDMAIYDLLAQYAKLPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
QYLGGYHSEIETDYTVSVNTVEEMVRDANQYKLNGFSILKVKVGKDQIETDMERIKAIRQ
HHHCCHHHCCCCCEEEEHHHHHHHHHCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHH
SVGNEVRIRLDANQGWNPKEAVKVIQKMEDLQLNIELVEQPVIAKDLEGLKFVTDRTETP
HCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEHHHCCCHHHHHCCCCEEECCCCCCC
IMADEAVFSIHDARQVLENKAADLINIKLMKSGGIHQARKIATLAQSYGIECMVGSMIET
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHH
KLGISAAAHFAASHPVVTRFDFDAPLMLSGDLVHGGVIYKGSHLSFSEDYGLGICGIRSE
HHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEECCEEEECCCCCCCCCCCCEEEHHHHH
FLRQNTMK
HHHHCCCC
>Mature Secondary Structure
MKITSVETFIGSVPLHTPFKTALRTVTVAESIIVKITSEDGYVGWGEAPPTHVITGESLA
CCCCCHHHHHCCCCCCCCHHHHHHHHHHEEEEEEEEECCCCEEECCCCCCCEEECCCHHH
SIEYAIHHVIKPFLIGNSLSTYERIFEQLQACMIGNTSAKAAVDMAIYDLLAQYAKLPLY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHH
QYLGGYHSEIETDYTVSVNTVEEMVRDANQYKLNGFSILKVKVGKDQIETDMERIKAIRQ
HHHCCHHHCCCCCEEEEHHHHHHHHHCCCCEEECCEEEEEEEECCHHHHHHHHHHHHHHH
SVGNEVRIRLDANQGWNPKEAVKVIQKMEDLQLNIELVEQPVIAKDLEGLKFVTDRTETP
HCCCEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEHHHCCCHHHHHCCCCEEECCCCCCC
IMADEAVFSIHDARQVLENKAADLINIKLMKSGGIHQARKIATLAQSYGIECMVGSMIET
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHH
KLGISAAAHFAASHPVVTRFDFDAPLMLSGDLVHGGVIYKGSHLSFSEDYGLGICGIRSE
HHHHHHHHHHHCCCCEEEEECCCCCEEEECCEEECCEEEECCCCCCCCCCCCEEEHHHHH
FLRQNTMK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]