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The map label for this gene is capI [H]

Identifier: 23100338

GI number: 23100338

Start: 2969989

End: 2971011

Strand: Reverse

Name: capI [H]

Synonym: OB2883

Alternate gene names: 23100338

Gene position: 2971011-2969989 (Counterclockwise)

Preceding gene: 23100339

Following gene: 23100337

Centisome position: 81.83

GC content: 35.48

Gene sequence:

>1023_bases
TTGACTAAAGAAAACAAGGTTTACTTAATAACCGGAGCAGCGGGATTTATCGGACATTTCTTATCTCGAAGACTATTAGA
TCAAGGGTTTAACGTAATCGGATTAGATAATGTAAATGATTATTATGATGTGAACCTTAAAGAAACAAGGCTTAAATTAT
TACAACCCTACAATAATTTTACGTTTATTAAAGGTGATATTTCGGATAAGGAATTAGTACTTAGTATATTTGAAGAGTAC
AAGCCTTCAATCGTAGTGAATTTAGCAGCACAGGCTGGGGTTCGTTATTCTATAGAGAACCCAGATGTATATATGCAAAG
TAATGTAATTGGTTTTTATAATATCTTAGAGGCGTGTCGCCATCATCTTGTAGACCACTTAATTTATGCATCATCAAGTT
CAGTATATGGTGCTAATAAAAAAGTGCCGTTTGAAGAGACGGACTTTGTAGATAATCCGGTCTCACTATATGCATCTACG
AAAAAGTCAAATGAATTAATGGCTCATACATATAGTCACCTGTATGGTATTCCTGCGACTGGACTACGTTTCTTTACGGT
CTATGGACCAATGGGCAGACCTGATATGGCTTACTTTGGCTTTACGAATAAGTTATTCCATGGAGAACCTATTCAAATCT
TTAACAACGGAGATTTTGAAAATGACTTGTATCGTGACTTCACATATATTGATGATATTGTGGAGGGAATTGAGCGTTTG
ATTCCTCATAGCCCAGTAGGAGAAGTACAACATAAGGTATACAATATTGGAAATAGTAATCCAGAAAAACTCATGGTATT
TATTGAGACATTAGAGAAGTGCTTAAGCAATTCTCTTGGTGAAAAAGTAGAATTTGAAAAGCATTTTGAACCTATCAAAC
CAGGTGATGTACCCGCAACGTATGCATCTACGAAACAATTATATGATGCTGTAGGGTTTAGACCGCAAACTTCTATTGAA
GAAGGCTTGCAGCAGTTTACAGACTGGTATGTGGAGTATTATGGAGTTAAAGTAGAAGTATAA

Upstream 100 bases:

>100_bases
AATATTCATCCAGTCAAAGAAATGGTAGAGGCTAGTGTTGAGTACTATTCAGTGGGAAGACCGTCGACTGTTGATACTCG
AGTAAAGGAGATGGAAATCG

Downstream 100 bases:

>100_bases
CTATAACTATATTTTTTAGAGAGGTGAAATGATTTTTCATCTCTCTTTTTTATAGAGAGGATGTATACTGTGAATGATAC
AAAGGGATTAATTCTCTATT

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTKENKVYLITGAAGFIGHFLSRRLLDQGFNVIGLDNVNDYYDVNLKETRLKLLQPYNNFTFIKGDISDKELVLSIFEEY
KPSIVVNLAAQAGVRYSIENPDVYMQSNVIGFYNILEACRHHLVDHLIYASSSSVYGANKKVPFEETDFVDNPVSLYAST
KKSNELMAHTYSHLYGIPATGLRFFTVYGPMGRPDMAYFGFTNKLFHGEPIQIFNNGDFENDLYRDFTYIDDIVEGIERL
IPHSPVGEVQHKVYNIGNSNPEKLMVFIETLEKCLSNSLGEKVEFEKHFEPIKPGDVPATYASTKQLYDAVGFRPQTSIE
EGLQQFTDWYVEYYGVKVEV

Sequences:

>Translated_340_residues
MTKENKVYLITGAAGFIGHFLSRRLLDQGFNVIGLDNVNDYYDVNLKETRLKLLQPYNNFTFIKGDISDKELVLSIFEEY
KPSIVVNLAAQAGVRYSIENPDVYMQSNVIGFYNILEACRHHLVDHLIYASSSSVYGANKKVPFEETDFVDNPVSLYAST
KKSNELMAHTYSHLYGIPATGLRFFTVYGPMGRPDMAYFGFTNKLFHGEPIQIFNNGDFENDLYRDFTYIDDIVEGIERL
IPHSPVGEVQHKVYNIGNSNPEKLMVFIETLEKCLSNSLGEKVEFEKHFEPIKPGDVPATYASTKQLYDAVGFRPQTSIE
EGLQQFTDWYVEYYGVKVEV
>Mature_339_residues
TKENKVYLITGAAGFIGHFLSRRLLDQGFNVIGLDNVNDYYDVNLKETRLKLLQPYNNFTFIKGDISDKELVLSIFEEYK
PSIVVNLAAQAGVRYSIENPDVYMQSNVIGFYNILEACRHHLVDHLIYASSSSVYGANKKVPFEETDFVDNPVSLYASTK
KSNELMAHTYSHLYGIPATGLRFFTVYGPMGRPDMAYFGFTNKLFHGEPIQIFNNGDFENDLYRDFTYIDDIVEGIERLI
PHSPVGEVQHKVYNIGNSNPEKLMVFIETLEKCLSNSLGEKVEFEKHFEPIKPGDVPATYASTKQLYDAVGFRPQTSIEE
GLQQFTDWYVEYYGVKVEV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=28.3132530120482, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI56237023, Length=345, Percent_Identity=23.1884057971014, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI56118217, Length=345, Percent_Identity=23.1884057971014, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI189083684, Length=345, Percent_Identity=23.1884057971014, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI42516563, Length=343, Percent_Identity=25.3644314868805, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=26.0744985673352, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788353, Length=344, Percent_Identity=23.546511627907, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786974, Length=342, Percent_Identity=25.4385964912281, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788589, Length=356, Percent_Identity=25.2808988764045, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788366, Length=190, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=23.2091690544413, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=352, Percent_Identity=22.7272727272727, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=24.8554913294798, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=24.9258160237389, Blast_Score=78, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=140, Percent_Identity=30, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19923002, Length=339, Percent_Identity=26.5486725663717, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=26.7062314540059, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38803; Mature: 38672

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKENKVYLITGAAGFIGHFLSRRLLDQGFNVIGLDNVNDYYDVNLKETRLKLLQPYNNF
CCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHHHHCCCCCE
TFIKGDISDKELVLSIFEEYKPSIVVNLAAQAGVRYSIENPDVYMQSNVIGFYNILEACR
EEEECCCCCHHHHHHHHHHCCCEEEEEEHHHCCCEEEECCCCEEEECCCHHHHHHHHHHH
HHLVDHLIYASSSSVYGANKKVPFEETDFVDNPVSLYASTKKSNELMAHTYSHLYGIPAT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCCCC
GLRFFTVYGPMGRPDMAYFGFTNKLFHGEPIQIFNNGDFENDLYRDFTYIDDIVEGIERL
CEEEEEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IPHSPVGEVQHKVYNIGNSNPEKLMVFIETLEKCLSNSLGEKVEFEKHFEPIKPGDVPAT
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCH
YASTKQLYDAVGFRPQTSIEEGLQQFTDWYVEYYGVKVEV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEC
>Mature Secondary Structure 
TKENKVYLITGAAGFIGHFLSRRLLDQGFNVIGLDNVNDYYDVNLKETRLKLLQPYNNF
CCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCHHHHHHHHHCCCCCE
TFIKGDISDKELVLSIFEEYKPSIVVNLAAQAGVRYSIENPDVYMQSNVIGFYNILEACR
EEEECCCCCHHHHHHHHHHCCCEEEEEEHHHCCCEEEECCCCEEEECCCHHHHHHHHHHH
HHLVDHLIYASSSSVYGANKKVPFEETDFVDNPVSLYASTKKSNELMAHTYSHLYGIPAT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCHHHHHHHHHHCCCCCC
GLRFFTVYGPMGRPDMAYFGFTNKLFHGEPIQIFNNGDFENDLYRDFTYIDDIVEGIERL
CEEEEEEECCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
IPHSPVGEVQHKVYNIGNSNPEKLMVFIETLEKCLSNSLGEKVEFEKHFEPIKPGDVPAT
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCH
YASTKQLYDAVGFRPQTSIEEGLQQFTDWYVEYYGVKVEV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]