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The map label for this gene is pdhC [H]

Identifier: 23100330

GI number: 23100330

Start: 2960990

End: 2962252

Strand: Reverse

Name: pdhC [H]

Synonym: OB2875

Alternate gene names: 23100330

Gene position: 2962252-2960990 (Counterclockwise)

Preceding gene: 23100331

Following gene: 23100329

Centisome position: 81.59

GC content: 38.95

Gene sequence:

>1263_bases
ATGGTAGAAGTTAAATTGCATGATATTGGAGAAGGGATGACAGAGGGGGATATCCTGACTTATTTTATTCAAGAAGGGGA
TCAGGTTGAGGAAGATCAGCCCATTGTAGAAATGCAGACAGAAAAAATGGTTGCTGAAATAACAGCGCCAGCCAAAGGGA
CAGTAAAAGAAATTTTTATTGCTGAAGGAACAACGATTTCAGTCGGTACAACGATTATGACGATTGAATCAGAGGATGCA
ATGGAGAAAACGAAGTCTTCAGAAATACAACGAGCAGAAGGTAATCAAGCTACACAATTAAGCGCGAGTGATAACCAACA
TACAGAAACGAAACAGAAAAACGGGCCTAAACGAATAAAAGCATCACCATACACTAGGAAGGTTGCTAGAGAACTTGATG
TAGATATTGAATTGGTAGAAGGAACTGGCAAAGATGGACGCATTATGATTGAGGATGTTCAGCAGTTTTCTCAGAATAGA
GAGAGCGCTGCGACAAAGGTTAAGCCAGAAGTAGAACAGTTACAAAACCAATTCTTTCAAGAGACAGAAGAACAGGTGGA
TGCTAAGGAAGAGGAAGCAGAGATTATTCCATTCAAAGGCCGGCGCAAACAAATTGCCAAAAAAATGACTACATCTATTT
ATACAATCCCACATGTTCATCACATGGAAGAAGTTGATATGACTGAGTTACTGGAGTTCCGAAAAGAAATTAAGTCCGAT
GCGGATATATCTGTTGCAGCGTTTTTTATTAAAGCGCTAACAATAGCGTTGAAAGAATATCCAATCTTTAATGCTAAGCT
TCATGAGGAAAAAGAGGAAATTAGATTGGAAAAAGGAATTCATATGGGAATTGCAACGGATACAGAAGAAGGGTTAATTG
TTCCGGTTATTCAGAGCGCTGATATAAAGTCAATCCGCACCATTCACCGAGAAATGAAGGAACTGATGAAAAAAGCAAAA
GAAAATACACTATCTTTGAAAGAGATGACGGGAAGCACCTTTACGATCAGCAATGTTGGACCAATGGGAAGTATCGGTGC
AACACCAATTATCAATTATCCAGAAGTCGCGTTAATGGCTTTTCACAAAACGAAAAAAGCACCCGTGGTAAACGATAATG
ACGAGATCGTTATCCGATCGATGATGAATGTGACACTAACATTTGATCATCGTGTCACCGATGGAGGAAACGCAATAGCC
TTTACAAATAAGTTCAAAGCATTAATCGAAAATCCAAGATTACTACTAATAGAACTCCGTTAA

Upstream 100 bases:

>100_bases
ATGTTCCATTTTGGGCTTTGGAAGAACATAATATCCCAACACCAGCACGTGTAATGGATGCGATTAACCAAGTGATTAAT
TTTTAAGGGAGGTGGAACGA

Downstream 100 bases:

>100_bases
TCTATCAAATAAAAAATGAAGGAGATGCATTATGTTTCGAATTAAATCTGAACAAATGCCGTCTTTTCTAGAGGCTATCC
TTGTAATCACAGCTATCATC

Product: pyruvate dehydrogenase E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MVEVKLHDIGEGMTEGDILTYFIQEGDQVEEDQPIVEMQTEKMVAEITAPAKGTVKEIFIAEGTTISVGTTIMTIESEDA
MEKTKSSEIQRAEGNQATQLSASDNQHTETKQKNGPKRIKASPYTRKVARELDVDIELVEGTGKDGRIMIEDVQQFSQNR
ESAATKVKPEVEQLQNQFFQETEEQVDAKEEEAEIIPFKGRRKQIAKKMTTSIYTIPHVHHMEEVDMTELLEFRKEIKSD
ADISVAAFFIKALTIALKEYPIFNAKLHEEKEEIRLEKGIHMGIATDTEEGLIVPVIQSADIKSIRTIHREMKELMKKAK
ENTLSLKEMTGSTFTISNVGPMGSIGATPIINYPEVALMAFHKTKKAPVVNDNDEIVIRSMMNVTLTFDHRVTDGGNAIA
FTNKFKALIENPRLLLIELR

Sequences:

>Translated_420_residues
MVEVKLHDIGEGMTEGDILTYFIQEGDQVEEDQPIVEMQTEKMVAEITAPAKGTVKEIFIAEGTTISVGTTIMTIESEDA
MEKTKSSEIQRAEGNQATQLSASDNQHTETKQKNGPKRIKASPYTRKVARELDVDIELVEGTGKDGRIMIEDVQQFSQNR
ESAATKVKPEVEQLQNQFFQETEEQVDAKEEEAEIIPFKGRRKQIAKKMTTSIYTIPHVHHMEEVDMTELLEFRKEIKSD
ADISVAAFFIKALTIALKEYPIFNAKLHEEKEEIRLEKGIHMGIATDTEEGLIVPVIQSADIKSIRTIHREMKELMKKAK
ENTLSLKEMTGSTFTISNVGPMGSIGATPIINYPEVALMAFHKTKKAPVVNDNDEIVIRSMMNVTLTFDHRVTDGGNAIA
FTNKFKALIENPRLLLIELR
>Mature_420_residues
MVEVKLHDIGEGMTEGDILTYFIQEGDQVEEDQPIVEMQTEKMVAEITAPAKGTVKEIFIAEGTTISVGTTIMTIESEDA
MEKTKSSEIQRAEGNQATQLSASDNQHTETKQKNGPKRIKASPYTRKVARELDVDIELVEGTGKDGRIMIEDVQQFSQNR
ESAATKVKPEVEQLQNQFFQETEEQVDAKEEEAEIIPFKGRRKQIAKKMTTSIYTIPHVHHMEEVDMTELLEFRKEIKSD
ADISVAAFFIKALTIALKEYPIFNAKLHEEKEEIRLEKGIHMGIATDTEEGLIVPVIQSADIKSIRTIHREMKELMKKAK
ENTLSLKEMTGSTFTISNVGPMGSIGATPIINYPEVALMAFHKTKKAPVVNDNDEIVIRSMMNVTLTFDHRVTDGGNAIA
FTNKFKALIENPRLLLIELR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=433, Percent_Identity=32.3325635103926, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI31711992, Length=445, Percent_Identity=28.5393258426966, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI19923748, Length=224, Percent_Identity=36.6071428571429, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=26.3982102908277, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI203098816, Length=447, Percent_Identity=26.3982102908277, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI260898739, Length=139, Percent_Identity=34.5323741007194, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786305, Length=435, Percent_Identity=31.7241379310345, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786946, Length=424, Percent_Identity=26.6509433962264, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=435, Percent_Identity=28.735632183908, Blast_Score=211, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI25146366, Length=437, Percent_Identity=27.0022883295195, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=449, Percent_Identity=27.6169265033408, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=230, Percent_Identity=32.6086956521739, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=424, Percent_Identity=29.2452830188679, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=439, Percent_Identity=25.7403189066059, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=29.2740046838407, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=33.0357142857143, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=31.1688311688312, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20129315, Length=226, Percent_Identity=31.858407079646, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 47224; Mature: 47224

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVKLHDIGEGMTEGDILTYFIQEGDQVEEDQPIVEMQTEKMVAEITAPAKGTVKEIFI
CEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
AEGTTISVGTTIMTIESEDAMEKTKSSEIQRAEGNQATQLSASDNQHTETKQKNGPKRIK
ECCCEEEECEEEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCCCCCCCC
ASPYTRKVARELDVDIELVEGTGKDGRIMIEDVQQFSQNRESAATKVKPEVEQLQNQFFQ
CCCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ETEEQVDAKEEEAEIIPFKGRRKQIAKKMTTSIYTIPHVHHMEEVDMTELLEFRKEIKSD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHCCHHHHHHHHHHHCCC
ADISVAAFFIKALTIALKEYPIFNAKLHEEKEEIRLEKGIHMGIATDTEEGLIVPVIQSA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCC
DIKSIRTIHREMKELMKKAKENTLSLKEMTGSTFTISNVGPMGSIGATPIINYPEVALMA
CHHHHHHHHHHHHHHHHHHHHCCEEHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
FHKTKKAPVVNDNDEIVIRSMMNVTLTFDHRVTDGGNAIAFTNKFKALIENPRLLLIELR
HHHCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEC
>Mature Secondary Structure
MVEVKLHDIGEGMTEGDILTYFIQEGDQVEEDQPIVEMQTEKMVAEITAPAKGTVKEIFI
CEEEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
AEGTTISVGTTIMTIESEDAMEKTKSSEIQRAEGNQATQLSASDNQHTETKQKNGPKRIK
ECCCEEEECEEEEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCCCCCCCC
ASPYTRKVARELDVDIELVEGTGKDGRIMIEDVQQFSQNRESAATKVKPEVEQLQNQFFQ
CCCHHHHHHHHCCCEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ETEEQVDAKEEEAEIIPFKGRRKQIAKKMTTSIYTIPHVHHMEEVDMTELLEFRKEIKSD
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCHHHHHHCCHHHHHHHHHHHCCC
ADISVAAFFIKALTIALKEYPIFNAKLHEEKEEIRLEKGIHMGIATDTEEGLIVPVIQSA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCC
DIKSIRTIHREMKELMKKAKENTLSLKEMTGSTFTISNVGPMGSIGATPIINYPEVALMA
CHHHHHHHHHHHHHHHHHHHHCCEEHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHH
FHKTKKAPVVNDNDEIVIRSMMNVTLTFDHRVTDGGNAIAFTNKFKALIENPRLLLIELR
HHHCCCCCCCCCCCCEEEEEEEEEEEEEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA