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The map label for this gene is hpaG [H]

Identifier: 23100321

GI number: 23100321

Start: 2950785

End: 2951576

Strand: Reverse

Name: hpaG [H]

Synonym: OB2866

Alternate gene names: 23100321

Gene position: 2951576-2950785 (Counterclockwise)

Preceding gene: 23100322

Following gene: 23100320

Centisome position: 81.3

GC content: 35.86

Gene sequence:

>792_bases
ATGAGTAGTGTGAAATTTAAGCACCAAGGAGTTCAACAATTAAAAGAAGGAATTTATCAACCTGATCAAAAGCTTTTGAA
ATTCGATGGAAAGCAATGTTCTTTGGATGATTTATCATTGGATGTACCTATAAATGGAACAGTTTATGGAACATTACTGA
ATTATAAAGGCGAATATGCCGAGTTAGAGTCTTCTATGAATGAGGATCCATATAAAACTCCACCAAAAGCTCCTATTTTA
TACATAAAGCCGATTAATACACATATCGGATATGGTATGACAATTCCGTTGCCAGAAGGAGTAGAAAAGGTATCTATTGG
GGCTGCTTTGGGAGTCGTTATTGGTAAACCAGCTAAAAACATCAGAAAAGAAAACGCTCTAGATTATGTGGCTGGTTATA
CGATCGTAAATGATGTAAGTATACCAAATGAGAGTTATTATCGTCCTGCATATAAAGAAAAAGCAAGGGATGGATTTTGC
CCGATAGGTCCATGGGTTCTTCGTCGTGATGCTATAGAAAATCCAAATGAACTTGGGATTCGTGTTTACATCAATGGCGA
AATAAAACAGGAAAATACAACGTCAAATTTAATCCGTTCTGTTGAACAATTAATGGAAGAGGTAACAGATTTCATGACGC
TTTATGAAGGTGATGTATTATTGGTTGGTGTTCCGGAAAACGCTCCAATTGCAAAAGAAAATGATCGCGTTATCATTGAG
ATCGACAATATAGGAACTTTAGTAAATAAGGTAGAAAAAGAGAAAGTAGATGTAGGAGGCGTTATGGTATGA

Upstream 100 bases:

>100_bases
GATTTATATGAGGAAATTGAATGAAGACCAGTAAGCTACTGGTCTCTTTTTCTTCATTTAGACTGTACATTTTCTAAAAA
CATTAAAGGAGTGCGGTTAT

Downstream 100 bases:

>100_bases
GGCACGCACGAATAGCGTATAATGGTTTAATTCAACATGCGGTTGAAACAAACGAAGGAATTAAATTAGAAGACGGTCGT
ATTGTGAATGAGTCACAGGT

Product: 4-hydroxyphenylacetate degradation decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSSVKFKHQGVQQLKEGIYQPDQKLLKFDGKQCSLDDLSLDVPINGTVYGTLLNYKGEYAELESSMNEDPYKTPPKAPIL
YIKPINTHIGYGMTIPLPEGVEKVSIGAALGVVIGKPAKNIRKENALDYVAGYTIVNDVSIPNESYYRPAYKEKARDGFC
PIGPWVLRRDAIENPNELGIRVYINGEIKQENTTSNLIRSVEQLMEEVTDFMTLYEGDVLLVGVPENAPIAKENDRVIIE
IDNIGTLVNKVEKEKVDVGGVMV

Sequences:

>Translated_263_residues
MSSVKFKHQGVQQLKEGIYQPDQKLLKFDGKQCSLDDLSLDVPINGTVYGTLLNYKGEYAELESSMNEDPYKTPPKAPIL
YIKPINTHIGYGMTIPLPEGVEKVSIGAALGVVIGKPAKNIRKENALDYVAGYTIVNDVSIPNESYYRPAYKEKARDGFC
PIGPWVLRRDAIENPNELGIRVYINGEIKQENTTSNLIRSVEQLMEEVTDFMTLYEGDVLLVGVPENAPIAKENDRVIIE
IDNIGTLVNKVEKEKVDVGGVMV
>Mature_262_residues
SSVKFKHQGVQQLKEGIYQPDQKLLKFDGKQCSLDDLSLDVPINGTVYGTLLNYKGEYAELESSMNEDPYKTPPKAPILY
IKPINTHIGYGMTIPLPEGVEKVSIGAALGVVIGKPAKNIRKENALDYVAGYTIVNDVSIPNESYYRPAYKEKARDGFCP
IGPWVLRRDAIENPNELGIRVYINGEIKQENTTSNLIRSVEQLMEEVTDFMTLYEGDVLLVGVPENAPIAKENDRVIIEI
DNIGTLVNKVEKEKVDVGGVMV

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=187, Percent_Identity=33.6898395721925, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI40786394, Length=187, Percent_Identity=33.6898395721925, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI13654274, Length=205, Percent_Identity=29.2682926829268, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI215422413, Length=189, Percent_Identity=29.1005291005291, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI66348062, Length=189, Percent_Identity=29.1005291005291, Blast_Score=90, Evalue=2e-18,
Organism=Escherichia coli, GI1787428, Length=209, Percent_Identity=25.3588516746411, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557057, Length=193, Percent_Identity=34.7150259067358, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571789, Length=185, Percent_Identity=31.8918918918919, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24663695, Length=224, Percent_Identity=27.2321428571429, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572127, Length=228, Percent_Identity=27.6315789473684, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 29234; Mature: 29103

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVKFKHQGVQQLKEGIYQPDQKLLKFDGKQCSLDDLSLDVPINGTVYGTLLNYKGEYA
CCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECCCCHHH
ELESSMNEDPYKTPPKAPILYIKPINTHIGYGMTIPLPEGVEKVSIGAALGVVIGKPAKN
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCHHH
IRKENALDYVAGYTIVNDVSIPNESYYRPAYKEKARDGFCPIGPWVLRRDAIENPNELGI
HHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEE
RVYINGEIKQENTTSNLIRSVEQLMEEVTDFMTLYEGDVLLVGVPENAPIAKENDRVIIE
EEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEE
IDNIGTLVNKVEKEKVDVGGVMV
ECCHHHHHHHHHHHHHCCCCEEC
>Mature Secondary Structure 
SSVKFKHQGVQQLKEGIYQPDQKLLKFDGKQCSLDDLSLDVPINGTVYGTLLNYKGEYA
CCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECCCCHHH
ELESSMNEDPYKTPPKAPILYIKPINTHIGYGMTIPLPEGVEKVSIGAALGVVIGKPAKN
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCHHHHHHHHEEEEECCCHHH
IRKENALDYVAGYTIVNDVSIPNESYYRPAYKEKARDGFCPIGPWVLRRDAIENPNELGI
HHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCCEE
RVYINGEIKQENTTSNLIRSVEQLMEEVTDFMTLYEGDVLLVGVPENAPIAKENDRVIIE
EEEECCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEE
IDNIGTLVNKVEKEKVDVGGVMV
ECCHHHHHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550403 [H]