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The map label for this gene is hpaG [H]

Identifier: 23100320

GI number: 23100320

Start: 2950027

End: 2950788

Strand: Reverse

Name: hpaG [H]

Synonym: OB2865

Alternate gene names: 23100320

Gene position: 2950788-2950027 (Counterclockwise)

Preceding gene: 23100321

Following gene: 23100319

Centisome position: 81.28

GC content: 39.76

Gene sequence:

>762_bases
ATGAGGCACGCACGAATAGCGTATAATGGTTTAATTCAACATGCGGTTGAAACAAACGAAGGAATTAAATTAGAAGACGG
TCGTATTGTGAATGAGTCACAGGTCACATGGCTACCACCGGTAGAACCGAAAACGGTATTCACACTCGGTCTAAACTATG
CGGATCATGCGGCAGAGCTTTCATTTAAAGAAGCACCGAAAGAGCCATTGGTATTTTTAAAAGGACCAAATACGTTCATT
GGTCATCTTGGGGAAACGTATCGTCCAAGCGATGTAACATTTATGCACTATGAATGTGAACTAGCGGTCGTGATCGGTAA
AACAGCGAAAAACGTTAAAAAAGAGGAAGCATATAATTATGTTCGTGGCTATACGGTGGCGAATGATTACGCTCTTCGTG
ATTATCTAGAAAACTACTATCGACCAAACCTTCGTGTGAAAAATCGGGATACATGTACCCCAATTGGTCCATGGGTCGTG
GATAAAGAAGATATTGAAGATCCAATGAATTTACAATTGACTACTTATGTTAATGGTGAAATCACACAGCAAGGTAGTAC
TGCAGACATGATCTTCTCTGTACCTCATCTTATTGAGTATTTAAGTGGACTTATGACATTAAATCCAGGAGATATTATTC
TGACTGGAACACCAAAAGGGTCTGTTGATACAAAGGTTGGCGATGAAGTAATAACAGAAGTAGAAGGTGTAGGCAGACTT
GTGAATACAATCGTTGGGGAGAAAACCTTTCAATCTATCTAA

Upstream 100 bases:

>100_bases
TTGCAAAAGAAAATGATCGCGTTATCATTGAGATCGACAATATAGGAACTTTAGTAAATAAGGTAGAAAAAGAGAAAGTA
GATGTAGGAGGCGTTATGGT

Downstream 100 bases:

>100_bases
TTTAAGTGAAGATAACTTTATGAATAAAGTAAGGGGGATTCACGATGCCACATCTGATAATCGAATACACAGCCAATCTA
AAAGAAGAAACCGATATGAT

Product: 4-hydroxyphenylacetate degradation decarboxylase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MRHARIAYNGLIQHAVETNEGIKLEDGRIVNESQVTWLPPVEPKTVFTLGLNYADHAAELSFKEAPKEPLVFLKGPNTFI
GHLGETYRPSDVTFMHYECELAVVIGKTAKNVKKEEAYNYVRGYTVANDYALRDYLENYYRPNLRVKNRDTCTPIGPWVV
DKEDIEDPMNLQLTTYVNGEITQQGSTADMIFSVPHLIEYLSGLMTLNPGDIILTGTPKGSVDTKVGDEVITEVEGVGRL
VNTIVGEKTFQSI

Sequences:

>Translated_253_residues
MRHARIAYNGLIQHAVETNEGIKLEDGRIVNESQVTWLPPVEPKTVFTLGLNYADHAAELSFKEAPKEPLVFLKGPNTFI
GHLGETYRPSDVTFMHYECELAVVIGKTAKNVKKEEAYNYVRGYTVANDYALRDYLENYYRPNLRVKNRDTCTPIGPWVV
DKEDIEDPMNLQLTTYVNGEITQQGSTADMIFSVPHLIEYLSGLMTLNPGDIILTGTPKGSVDTKVGDEVITEVEGVGRL
VNTIVGEKTFQSI
>Mature_253_residues
MRHARIAYNGLIQHAVETNEGIKLEDGRIVNESQVTWLPPVEPKTVFTLGLNYADHAAELSFKEAPKEPLVFLKGPNTFI
GHLGETYRPSDVTFMHYECELAVVIGKTAKNVKKEEAYNYVRGYTVANDYALRDYLENYYRPNLRVKNRDTCTPIGPWVV
DKEDIEDPMNLQLTTYVNGEITQQGSTADMIFSVPHLIEYLSGLMTLNPGDIILTGTPKGSVDTKVGDEVITEVEGVGRL
VNTIVGEKTFQSI

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=226, Percent_Identity=39.8230088495575, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI40786394, Length=222, Percent_Identity=40.5405405405405, Blast_Score=156, Evalue=1e-38,
Organism=Homo sapiens, GI215422413, Length=200, Percent_Identity=37, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI66348062, Length=197, Percent_Identity=37.5634517766497, Blast_Score=127, Evalue=7e-30,
Organism=Homo sapiens, GI13654274, Length=197, Percent_Identity=37.5634517766497, Blast_Score=127, Evalue=9e-30,
Organism=Escherichia coli, GI1787428, Length=195, Percent_Identity=38.4615384615385, Blast_Score=124, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557057, Length=188, Percent_Identity=37.2340425531915, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324161, Length=225, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28572127, Length=228, Percent_Identity=41.2280701754386, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24663695, Length=238, Percent_Identity=34.453781512605, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28571789, Length=207, Percent_Identity=38.6473429951691, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28312; Mature: 28312

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHARIAYNGLIQHAVETNEGIKLEDGRIVNESQVTWLPPVEPKTVFTLGLNYADHAAEL
CCCCHHHHHHHHHHHHHCCCCEEECCCEEECCCCEEEECCCCCCEEEEEECCCCCHHHHC
SFKEAPKEPLVFLKGPNTFIGHLGETYRPSDVTFMHYECELAVVIGKTAKNVKKEEAYNY
CHHHCCCCCEEEEECCCHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHH
VRGYTVANDYALRDYLENYYRPNLRVKNRDTCTPIGPWVVDKEDIEDPMNLQLTTYVNGE
HHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECHHHCCCCCCEEEEEEECCE
ITQQGSTADMIFSVPHLIEYLSGLMTLNPGDIILTGTPKGSVDTKVGDEVITEVEGVGRL
EECCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
VNTIVGEKTFQSI
HHHHHCCHHHHCC
>Mature Secondary Structure
MRHARIAYNGLIQHAVETNEGIKLEDGRIVNESQVTWLPPVEPKTVFTLGLNYADHAAEL
CCCCHHHHHHHHHHHHHCCCCEEECCCEEECCCCEEEECCCCCCEEEEEECCCCCHHHHC
SFKEAPKEPLVFLKGPNTFIGHLGETYRPSDVTFMHYECELAVVIGKTAKNVKKEEAYNY
CHHHCCCCCEEEEECCCHHHHCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHHHHHH
VRGYTVANDYALRDYLENYYRPNLRVKNRDTCTPIGPWVVDKEDIEDPMNLQLTTYVNGE
HHCEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEECHHHCCCCCCEEEEEEECCE
ITQQGSTADMIFSVPHLIEYLSGLMTLNPGDIILTGTPKGSVDTKVGDEVITEVEGVGRL
EECCCCCHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHH
VNTIVGEKTFQSI
HHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA