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The map label for this gene is gudD [H]

Identifier: 23100291

GI number: 23100291

Start: 2918600

End: 2919964

Strand: Reverse

Name: gudD [H]

Synonym: OB2836

Alternate gene names: 23100291

Gene position: 2919964-2918600 (Counterclockwise)

Preceding gene: 23100292

Following gene: 23100289

Centisome position: 80.43

GC content: 39.78

Gene sequence:

>1365_bases
ATGGGGCAGCAAACGATTGATGAAAACAAAAATATAACAGTACACACTCCAATCATTCAAGAGATGCGTGTTATACCTGT
AGCTGGAAAAGATAGCATGCTACTTAATTTAAGCGGTGCACATAGTGCTTTTTTTACAAGAAATATTGTAATTCTCAAAG
ATAGTGCCGGAAATATTGGCGTAGGTGAGGTGCCAGGTGGAGAACCGATAAGACAAACTTTAGAGGATACGAAAAGTCTA
GTTCTACAAAGAAAAATAGGAGAATATAAAGACATATTAAATGAAATAAGAATAAAGTTCGGAAATCGCGACCAAGGTGG
AAGAGGTAATCAAACATTTGATTTACGAACGACTATTCATGCAGTTACTGCACTGGAAAGTTCCCTGCTTGATTTACTTG
GAAAGTTCCTAGAGGTTCCTGTTGCTTCATTGTTAGGGGAAGGTAGACAGCGCGAAGAAGTAGAAATGTTAGGTTATCTA
TTCTATGTCGGTGACCGTAATCAAACAGATTTACCTTATGAAAGTGATGAAAATGCCCAGGATGAATGGTTCCGATTACG
TCATGAAGAGGCATTAACTCCGGAGTCGATCGTGCGATTAGCAGAAGCTGCACATAAAAGATATGGTTTTAAGGATTTTA
AACTTAAAGGAGGTGTGCTTCGAGGCGAAGAAGAAATGGAAGCTATTATTGCATTAAAAGAAAAGTTTCCTAACGCCAGA
ATCACACTTGATCCAAACGGAGGTTGGCTGCTTGAAGATGCCATTCGCCTCTGCAAAGATAAACACGATATTTTGGCATA
TGCTGAGGACCCATGCGGTTCAGAGGGAAGCTATTCTGCACGGGAAGTGATGGCAGAGTTTCGTAAAGCTACTGGACTGG
CGACTGCAACCAATATGATTGCGACCGATTGGCGACAAATGGGGCATGCTATTCAACTAAATGCCGTAGATATTCCTTTA
GCAGATCCACATTTCTGGACGATGCAAGGATCTGTGCGTGTCGCACAATTATGCCATGAGTGGGGCTTAACTTGGGGTTC
ACATTCCAATAATCATTTTGATATCTCACTTGCCATGTTTACACATGTAGCTGCAGCAGCACCCGGTAATATCACAGCAA
TTGATACACATTGGATATGGCAAGATGGCCAAGGTCTAACCAAAAATCCATTCCAAATTAAAGATGGCAAAGTAAAAGTA
CCTAAAACACCAGGATTAGGAATAGAACTTGATATGGAAAAGGTAGAAAAAGCTCACCAATTATATGTTAATAATAATTT
GGGTGTAAGAGATGATGCGAAAGCTATGCAATATTTAATTAAGGGATGGAAGTTTGATAGTAAAAAACCAGCATTCGTTC
GATAA

Upstream 100 bases:

>100_bases
CGGGACTGTCATGTTCGCAGACGGTCCTCTTGTTTTTTAGTCGGAGAGATGTATTTACAAGGATATATAAAATAGATAAA
TAAATGGAGGAGGTAGGCGT

Downstream 100 bases:

>100_bases
AAACGAGGAAGTGAATTCTTCTTCGTTTTTTTTGTGGTTTAGGAAGTCTGGGTTTACCAACAAAATTTAAAAAATAAAAA
GTGTCACTAAAACAATAAGC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MGQQTIDENKNITVHTPIIQEMRVIPVAGKDSMLLNLSGAHSAFFTRNIVILKDSAGNIGVGEVPGGEPIRQTLEDTKSL
VLQRKIGEYKDILNEIRIKFGNRDQGGRGNQTFDLRTTIHAVTALESSLLDLLGKFLEVPVASLLGEGRQREEVEMLGYL
FYVGDRNQTDLPYESDENAQDEWFRLRHEEALTPESIVRLAEAAHKRYGFKDFKLKGGVLRGEEEMEAIIALKEKFPNAR
ITLDPNGGWLLEDAIRLCKDKHDILAYAEDPCGSEGSYSAREVMAEFRKATGLATATNMIATDWRQMGHAIQLNAVDIPL
ADPHFWTMQGSVRVAQLCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQGLTKNPFQIKDGKVKV
PKTPGLGIELDMEKVEKAHQLYVNNNLGVRDDAKAMQYLIKGWKFDSKKPAFVR

Sequences:

>Translated_454_residues
MGQQTIDENKNITVHTPIIQEMRVIPVAGKDSMLLNLSGAHSAFFTRNIVILKDSAGNIGVGEVPGGEPIRQTLEDTKSL
VLQRKIGEYKDILNEIRIKFGNRDQGGRGNQTFDLRTTIHAVTALESSLLDLLGKFLEVPVASLLGEGRQREEVEMLGYL
FYVGDRNQTDLPYESDENAQDEWFRLRHEEALTPESIVRLAEAAHKRYGFKDFKLKGGVLRGEEEMEAIIALKEKFPNAR
ITLDPNGGWLLEDAIRLCKDKHDILAYAEDPCGSEGSYSAREVMAEFRKATGLATATNMIATDWRQMGHAIQLNAVDIPL
ADPHFWTMQGSVRVAQLCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQGLTKNPFQIKDGKVKV
PKTPGLGIELDMEKVEKAHQLYVNNNLGVRDDAKAMQYLIKGWKFDSKKPAFVR
>Mature_453_residues
GQQTIDENKNITVHTPIIQEMRVIPVAGKDSMLLNLSGAHSAFFTRNIVILKDSAGNIGVGEVPGGEPIRQTLEDTKSLV
LQRKIGEYKDILNEIRIKFGNRDQGGRGNQTFDLRTTIHAVTALESSLLDLLGKFLEVPVASLLGEGRQREEVEMLGYLF
YVGDRNQTDLPYESDENAQDEWFRLRHEEALTPESIVRLAEAAHKRYGFKDFKLKGGVLRGEEEMEAIIALKEKFPNARI
TLDPNGGWLLEDAIRLCKDKHDILAYAEDPCGSEGSYSAREVMAEFRKATGLATATNMIATDWRQMGHAIQLNAVDIPLA
DPHFWTMQGSVRVAQLCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQGLTKNPFQIKDGKVKVP
KTPGLGIELDMEKVEKAHQLYVNNNLGVRDDAKAMQYLIKGWKFDSKKPAFVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=69.5454545454545, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=445, Percent_Identity=62.0224719101124, Blast_Score=568, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50802; Mature: 50671

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQQTIDENKNITVHTPIIQEMRVIPVAGKDSMLLNLSGAHSAFFTRNIVILKDSAGNIG
CCCCCCCCCCCEEEECCHHHCCEEEEECCCCCEEEEECCCCCEEEEEEEEEEECCCCCCC
VGEVPGGEPIRQTLEDTKSLVLQRKIGEYKDILNEIRIKFGNRDQGGRGNQTFDLRTTIH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEHHHHHH
AVTALESSLLDLLGKFLEVPVASLLGEGRQREEVEMLGYLFYVGDRNQTDLPYESDENAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCH
DEWFRLRHEEALTPESIVRLAEAAHKRYGFKDFKLKGGVLRGEEEMEAIIALKEKFPNAR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHHHHHCCCCE
ITLDPNGGWLLEDAIRLCKDKHDILAYAEDPCGSEGSYSAREVMAEFRKATGLATATNMI
EEECCCCCEEHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ATDWRQMGHAIQLNAVDIPLADPHFWTMQGSVRVAQLCHEWGLTWGSHSNNHFDISLAMF
HHHHHHCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
THVAAAAPGNITAIDTHWIWQDGQGLTKNPFQIKDGKVKVPKTPGLGIELDMEKVEKAHQ
HHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEECCCEEECCCCCCCCEEECHHHHHHHHE
LYVNNNLGVRDDAKAMQYLIKGWKFDSKKPAFVR
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GQQTIDENKNITVHTPIIQEMRVIPVAGKDSMLLNLSGAHSAFFTRNIVILKDSAGNIG
CCCCCCCCCCEEEECCHHHCCEEEEECCCCCEEEEECCCCCEEEEEEEEEEECCCCCCC
VGEVPGGEPIRQTLEDTKSLVLQRKIGEYKDILNEIRIKFGNRDQGGRGNQTFDLRTTIH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEHHHHHH
AVTALESSLLDLLGKFLEVPVASLLGEGRQREEVEMLGYLFYVGDRNQTDLPYESDENAQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEEEEECCCCCCCCCCCCCCCCH
DEWFRLRHEEALTPESIVRLAEAAHKRYGFKDFKLKGGVLRGEEEMEAIIALKEKFPNAR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCEECCHHHHHHHHHHHHHCCCCE
ITLDPNGGWLLEDAIRLCKDKHDILAYAEDPCGSEGSYSAREVMAEFRKATGLATATNMI
EEECCCCCEEHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ATDWRQMGHAIQLNAVDIPLADPHFWTMQGSVRVAQLCHEWGLTWGSHSNNHFDISLAMF
HHHHHHCCCEEEEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
THVAAAAPGNITAIDTHWIWQDGQGLTKNPFQIKDGKVKVPKTPGLGIELDMEKVEKAHQ
HHHHHCCCCCEEEEEEEEEEECCCCCCCCCCEECCCEEECCCCCCCCEEECHHHHHHHHE
LYVNNNLGVRDDAKAMQYLIKGWKFDSKKPAFVR
EEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]