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The map label for this gene is opuD [H]

Identifier: 23100289

GI number: 23100289

Start: 2915283

End: 2916863

Strand: Reverse

Name: opuD [H]

Synonym: OB2834

Alternate gene names: 23100289

Gene position: 2916863-2915283 (Counterclockwise)

Preceding gene: 23100291

Following gene: 23100288

Centisome position: 80.34

GC content: 39.91

Gene sequence:

>1581_bases
ATGGCATTAGTAATGGTATTTATTATATGGGGAGCTTTTTTCCCTGCAAATGTTGAACATGTTTTAGGCATAGTTGATAC
CTATATTTCGAATACATTTGGCTGGTTTTACTTGCTAGTTACTACAGCATTTGTTTTGGTTGCCTTGTTTTTGATTTTTG
GTCCTTATGGAAAAATCAAGCTAGGAAAGCCTGACGACAAACCAGATTATAGTTATTTTACATGGTTTGCGTTTTTATTC
ACTGCCGGTATGGGTGTTGGACTCGTATTCTACGGGGTGACGGAACCAGTAACACATTATTATTCACCTCCTTCTGCGGA
ACCTGAAACAATTGCAGCTGCAGAAGAATCAATGCAGTACACTTTATTCCATTGGGGACTTCATCCATGGGCTACATACG
CTGTGCTGGCATTGGCATTAGCTTACTTTAAATTCCGCCATCGCGCACCTGCTTTGATTAGTTCCACTTTCGCACCACTT
TTTGGCGACCGTGTTAAAGGTCCATTGGGAATTGCCATCGATACACTAGCTGTTTTTGCTACTGTATTCGGTATTGCAAC
ATCTCTCGGACTTGGCGCAACACAAATTACAGCTGGTTTGAGCTTTACCTTTGATGGAATCGAAAATACGTTGACAACCA
ATTTAATCGTGATATTGATTGTCACTGTACTATTCATTCTTTCTGCTACCACTGGAATAAATAAAGGTATACGATATCTG
AGCTGGGCAAATGTTGTTCTTGCTATCGCATTAATGTTATTTGTGTTTGTTTTAGGATCCTCAGTACAAATGATTGAGTC
CTTTACAACAACTATTGGTAACTATATTCAAAATTTACCATCAATGACGTTAAACATGAATGCATTTACCGGAGAAAGAG
AGTTTCTTAATGCATGGACACTCTTTTACTGGGCATGGTGGATTGGATGGTCTCCATTTGTAGGAACCTTTATCGCGAGG
GTTTCTAGAGGAAGAACCATTCGTGAATTTGTTATTGGGGTAACTGCTGTTCCTGTAGTATTTAGTGCCTTATGGTTCTC
GATTTTCGGAGTTGCAGGGTTAGAAATGGATGCAGCTCAAGGCGGTATTATCCATCAATTAATGAATGAGTTAGGAAATG
AAGTAGCATTATTTGCATTCCTTGAGTCACAGCCTATGGCAGCTGTAGTGATCGGTGTCGCAGTACTCTTAATTGCTTCG
TTCTTTATTACTTCTGCGGATTCCGGTACATTCGTATTAAGTATGCTGACAACAGGAGGAAAATTAAACCCAGGCATAGG
AATCAAAGCTGTTTGGGGTATAATACTTGCTTCTATTGCTGCTGTTCTATTGTGGTCTGGTGGGCTTACAGCTCTCCAAA
TGGCGATGCTGATATCGGCATTCCCATTTGCAATCTTAATGGTCCTCATGTGTGTCTCACTAATCAAGGCATTGAGAAGT
GAGCATGGCATAATAACAATAGAGAAAAGACAACGTCAACATGATCCTCAGTTTAGAGAAAAACAACAAAAAGAACTCAA
AAAGATGCGGGAAGGATTTAAGGAAACACTTCCTGACGCTACGGAAGATAGAGATCTATAA

Upstream 100 bases:

>100_bases
TTTTACATGTTATCTGTTTATGTTATACTACAAGAGTTTTGACAAAGAAAGATAAGGAGGTCATATTATCAAATCAATTA
CACCTGTTTTTTGGTTATCT

Downstream 100 bases:

>100_bases
TATGAAAAACGGTCCAGGTAGGAGAATGCTTCAACCTGGACCGTTTTTTTGAAAAAGTGATTATGCGCTGTAGTTCTACA
TTAATATAGGTTTAGCTTTC

Product: glycine betaine transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MALVMVFIIWGAFFPANVEHVLGIVDTYISNTFGWFYLLVTTAFVLVALFLIFGPYGKIKLGKPDDKPDYSYFTWFAFLF
TAGMGVGLVFYGVTEPVTHYYSPPSAEPETIAAAEESMQYTLFHWGLHPWATYAVLALALAYFKFRHRAPALISSTFAPL
FGDRVKGPLGIAIDTLAVFATVFGIATSLGLGATQITAGLSFTFDGIENTLTTNLIVILIVTVLFILSATTGINKGIRYL
SWANVVLAIALMLFVFVLGSSVQMIESFTTTIGNYIQNLPSMTLNMNAFTGEREFLNAWTLFYWAWWIGWSPFVGTFIAR
VSRGRTIREFVIGVTAVPVVFSALWFSIFGVAGLEMDAAQGGIIHQLMNELGNEVALFAFLESQPMAAVVIGVAVLLIAS
FFITSADSGTFVLSMLTTGGKLNPGIGIKAVWGIILASIAAVLLWSGGLTALQMAMLISAFPFAILMVLMCVSLIKALRS
EHGIITIEKRQRQHDPQFREKQQKELKKMREGFKETLPDATEDRDL

Sequences:

>Translated_526_residues
MALVMVFIIWGAFFPANVEHVLGIVDTYISNTFGWFYLLVTTAFVLVALFLIFGPYGKIKLGKPDDKPDYSYFTWFAFLF
TAGMGVGLVFYGVTEPVTHYYSPPSAEPETIAAAEESMQYTLFHWGLHPWATYAVLALALAYFKFRHRAPALISSTFAPL
FGDRVKGPLGIAIDTLAVFATVFGIATSLGLGATQITAGLSFTFDGIENTLTTNLIVILIVTVLFILSATTGINKGIRYL
SWANVVLAIALMLFVFVLGSSVQMIESFTTTIGNYIQNLPSMTLNMNAFTGEREFLNAWTLFYWAWWIGWSPFVGTFIAR
VSRGRTIREFVIGVTAVPVVFSALWFSIFGVAGLEMDAAQGGIIHQLMNELGNEVALFAFLESQPMAAVVIGVAVLLIAS
FFITSADSGTFVLSMLTTGGKLNPGIGIKAVWGIILASIAAVLLWSGGLTALQMAMLISAFPFAILMVLMCVSLIKALRS
EHGIITIEKRQRQHDPQFREKQQKELKKMREGFKETLPDATEDRDL
>Mature_525_residues
ALVMVFIIWGAFFPANVEHVLGIVDTYISNTFGWFYLLVTTAFVLVALFLIFGPYGKIKLGKPDDKPDYSYFTWFAFLFT
AGMGVGLVFYGVTEPVTHYYSPPSAEPETIAAAEESMQYTLFHWGLHPWATYAVLALALAYFKFRHRAPALISSTFAPLF
GDRVKGPLGIAIDTLAVFATVFGIATSLGLGATQITAGLSFTFDGIENTLTTNLIVILIVTVLFILSATTGINKGIRYLS
WANVVLAIALMLFVFVLGSSVQMIESFTTTIGNYIQNLPSMTLNMNAFTGEREFLNAWTLFYWAWWIGWSPFVGTFIARV
SRGRTIREFVIGVTAVPVVFSALWFSIFGVAGLEMDAAQGGIIHQLMNELGNEVALFAFLESQPMAAVVIGVAVLLIASF
FITSADSGTFVLSMLTTGGKLNPGIGIKAVWGIILASIAAVLLWSGGLTALQMAMLISAFPFAILMVLMCVSLIKALRSE
HGIITIEKRQRQHDPQFREKQQKELKKMREGFKETLPDATEDRDL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=495, Percent_Identity=37.5757575757576, Blast_Score=348, Evalue=5e-97,
Organism=Escherichia coli, GI1786224, Length=472, Percent_Identity=28.6016949152542, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1788102, Length=459, Percent_Identity=30.2832244008715, Blast_Score=190, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 57775; Mature: 57643

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALVMVFIIWGAFFPANVEHVLGIVDTYISNTFGWFYLLVTTAFVLVALFLIFGPYGKIK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
LGKPDDKPDYSYFTWFAFLFTAGMGVGLVFYGVTEPVTHYYSPPSAEPETIAAAEESMQY
CCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
TLFHWGLHPWATYAVLALALAYFKFRHRAPALISSTFAPLFGDRVKGPLGIAIDTLAVFA
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TVFGIATSLGLGATQITAGLSFTFDGIENTLTTNLIVILIVTVLFILSATTGINKGIRYL
HHHHHHHHHCCCHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SWANVVLAIALMLFVFVLGSSVQMIESFTTTIGNYIQNLPSMTLNMNAFTGEREFLNAWT
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHH
LFYWAWWIGWSPFVGTFIARVSRGRTIREFVIGVTAVPVVFSALWFSIFGVAGLEMDAAQ
HHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GGIIHQLMNELGNEVALFAFLESQPMAAVVIGVAVLLIASFFITSADSGTFVLSMLTTGG
CHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHCCC
KLNPGIGIKAVWGIILASIAAVLLWSGGLTALQMAMLISAFPFAILMVLMCVSLIKALRS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EHGIITIEKRQRQHDPQFREKQQKELKKMREGFKETLPDATEDRDL
CCCEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]