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The map label for this gene is liaR [H]

Identifier: 23100277

GI number: 23100277

Start: 2901963

End: 2902595

Strand: Reverse

Name: liaR [H]

Synonym: OB2822

Alternate gene names: 23100277

Gene position: 2902595-2901963 (Counterclockwise)

Preceding gene: 23100278

Following gene: 23100276

Centisome position: 79.95

GC content: 37.76

Gene sequence:

>633_bases
ATGATTAAAGTTTTATTTGTCGATGATCATGAAATGGTACGAATAGGCGTATCTGCATATCTTTCTTCGCAACCAGATAT
TGATGTAATAGCAGAGGCAGATGATGGTACACAGGCTGTAGAACTTGCATTAGATCTGCGCCCAGATATTATTCTAATGG
ATCTTGTTATGAAGGAAATGGATGGAATTCAAGCAACAAAGAAGATAATAGAGGCATGGCCAGCAGCAAAAATTATTATT
GTAACGAGTTTCTTAGATGATGAAAAAGTATATCCTGCGTTAGAAGCAGGAGCGACAAGCTATATGCTGAAAACATCGAA
AGCAAGTGAAATTGCCAAAGCGATTCGTTCAACGTATGAAGGGCAGTCCATCTTGGAACCAGAAGTGACAGGGAAAATTA
TGAATCGTATGCGTACTGGGCAGAATACTGCACTTCACGAGCAATTAACAGAACGAGAAAAAGAAATATTATTATTAATG
GCAAAAGGAAAGTCGAATCAAGAAATTGCGAATGAATTGTTTATTGCCTTAAAAACCGTAAAAGTCCATGTGAGCAATAT
CTTAGGGAAGTTAGAGGTGCATGATCGTACGCAGGCTGTTATTTATGCATTTCAGAATAAGTTGGTGGAGTAA

Upstream 100 bases:

>100_bases
GCGTATGAAGTTGGCGGTACATTGAAGGTTATAAGTGTACCAAATGAAGGTACACGGCTTGAAGTAAAAGTCCCTATCTT
AAGAAAGGAAGATACATCCA

Downstream 100 bases:

>100_bases
TGTAATTCCTTATCATCAAGTTAGTTGTGAATATTTATTTTAAGGTCTTTCTTATTTTCTGGGGAAGACTTTTATTAGTT
GACTTCATATCTGTAATAGT

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIKVLFVDDHEMVRIGVSAYLSSQPDIDVIAEADDGTQAVELALDLRPDIILMDLVMKEMDGIQATKKIIEAWPAAKIII
VTSFLDDEKVYPALEAGATSYMLKTSKASEIAKAIRSTYEGQSILEPEVTGKIMNRMRTGQNTALHEQLTEREKEILLLM
AKGKSNQEIANELFIALKTVKVHVSNILGKLEVHDRTQAVIYAFQNKLVE

Sequences:

>Translated_210_residues
MIKVLFVDDHEMVRIGVSAYLSSQPDIDVIAEADDGTQAVELALDLRPDIILMDLVMKEMDGIQATKKIIEAWPAAKIII
VTSFLDDEKVYPALEAGATSYMLKTSKASEIAKAIRSTYEGQSILEPEVTGKIMNRMRTGQNTALHEQLTEREKEILLLM
AKGKSNQEIANELFIALKTVKVHVSNILGKLEVHDRTQAVIYAFQNKLVE
>Mature_210_residues
MIKVLFVDDHEMVRIGVSAYLSSQPDIDVIAEADDGTQAVELALDLRPDIILMDLVMKEMDGIQATKKIIEAWPAAKIII
VTSFLDDEKVYPALEAGATSYMLKTSKASEIAKAIRSTYEGQSILEPEVTGKIMNRMRTGQNTALHEQLTEREKEILLLM
AKGKSNQEIANELFIALKTVKVHVSNILGKLEVHDRTQAVIYAFQNKLVE

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=37.378640776699, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=31.4285714285714, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1787473, Length=207, Percent_Identity=36.231884057971, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786747, Length=203, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=29.0816326530612, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1788712, Length=207, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788550, Length=122, Percent_Identity=31.1475409836066, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI87082052, Length=115, Percent_Identity=28.695652173913, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788546, Length=204, Percent_Identity=25, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23400; Mature: 23400

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLFVDDHEMVRIGVSAYLSSQPDIDVIAEADDGTQAVELALDLRPDIILMDLVMKEM
CEEEEEECCCCEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
DGIQATKKIIEAWPAAKIIIVTSFLDDEKVYPALEAGATSYMLKTSKASEIAKAIRSTYE
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCHHEEEECCHHHHHHHHHHHHCC
GQSILEPEVTGKIMNRMRTGQNTALHEQLTEREKEILLLMAKGKSNQEIANELFIALKTV
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHH
KVHVSNILGKLEVHDRTQAVIYAFQNKLVE
HHHHHHHHHHCEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKVLFVDDHEMVRIGVSAYLSSQPDIDVIAEADDGTQAVELALDLRPDIILMDLVMKEM
CEEEEEECCCCEEEEHHHHHHCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHH
DGIQATKKIIEAWPAAKIIIVTSFLDDEKVYPALEAGATSYMLKTSKASEIAKAIRSTYE
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHCCCHHEEEECCHHHHHHHHHHHHCC
GQSILEPEVTGKIMNRMRTGQNTALHEQLTEREKEILLLMAKGKSNQEIANELFIALKTV
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHH
KVHVSNILGKLEVHDRTQAVIYAFQNKLVE
HHHHHHHHHHCEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]