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The map label for this gene is crr

Identifier: 23100213

GI number: 23100213

Start: 2829743

End: 2830240

Strand: Reverse

Name: crr

Synonym: OB2758

Alternate gene names: 23100213

Gene position: 2830240-2829743 (Counterclockwise)

Preceding gene: 23100214

Following gene: 23100212

Centisome position: 77.96

GC content: 36.35

Gene sequence:

>498_bases
ATGTTTAAGAACTTATTTAAAATCAAAGAGAGAGAGCCAAAAGAAATTACAGTAGTTGCACCGATCACAGGGGAAATCAT
ACCATTAGAGGAGGTTCCTGATCCAGTTTTCGCACAAAAGATGATGGGTGAGGGTGTCGCAGTGAAGCCTGCAAATGGAG
AAGTAATATCTCCAGTTGACGGAGAAGTTAAACTGGTTTTTCAAACGAAACATGCAATAATTGTTGAGGCAGAGAACAAT
GCTGAAATTCTAATTCATATTGGACTAGATACTGTAAATCTTGAAGGAGAAGGTTTTACTGCCCATGTTAAGGATGGAGA
TATAGTAAAGGTCGGCGACAAATTAATGAGCTTCGATATCGCAACCATTGAAGAAAAGGCAACGAGTAGTATTACACCTA
TAATCATTTCAAATACTGATAATGTAAGAGAAGTTAAAAATATAAAAGTAACAGAAGTTACAGCTGGTGAGAATCAGATT
TTACAAGTGATTAACTAG

Upstream 100 bases:

>100_bases
AATATAGTTATCCTTCTTACAATGGGATGTTTATTTTTAAATGGAAATACAAATGCAAACAATCAACTACGATATTAACA
AGCAAAAGGGGACTTGGGAG

Downstream 100 bases:

>100_bases
TTGAGGAGGAGATAATGCCATGCGAATTGGAGCAATTGAAGCAGGTGGTACAAAATTTGTTTGTGCGGTAGGTAGTCGAA
ATGGTGAGATTTTAGAACGT

Product: PTS system glucose-specific enzyme II A component

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MFKNLFKIKEREPKEITVVAPITGEIIPLEEVPDPVFAQKMMGEGVAVKPANGEVISPVDGEVKLVFQTKHAIIVEAENN
AEILIHIGLDTVNLEGEGFTAHVKDGDIVKVGDKLMSFDIATIEEKATSSITPIIISNTDNVREVKNIKVTEVTAGENQI
LQVIN

Sequences:

>Translated_165_residues
MFKNLFKIKEREPKEITVVAPITGEIIPLEEVPDPVFAQKMMGEGVAVKPANGEVISPVDGEVKLVFQTKHAIIVEAENN
AEILIHIGLDTVNLEGEGFTAHVKDGDIVKVGDKLMSFDIATIEEKATSSITPIIISNTDNVREVKNIKVTEVTAGENQI
LQVIN
>Mature_165_residues
MFKNLFKIKEREPKEITVVAPITGEIIPLEEVPDPVFAQKMMGEGVAVKPANGEVISPVDGEVKLVFQTKHAIIVEAENN
AEILIHIGLDTVNLEGEGFTAHVKDGDIVKVGDKLMSFDIATIEEKATSSITPIIISNTDNVREVKNIKVTEVTAGENQI
LQVIN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain

Homologues:

Organism=Escherichia coli, GI1788757, Length=163, Percent_Identity=41.1042944785276, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1790159, Length=155, Percent_Identity=44.5161290322581, Blast_Score=139, Evalue=9e-35,
Organism=Escherichia coli, GI1786894, Length=146, Percent_Identity=38.3561643835616, Blast_Score=116, Evalue=6e-28,

Paralogues:

None

Copy number: 3540 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PTGA_OCEIH (Q8CZD5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693680.1
- ProteinModelPortal:   Q8CZD5
- SMR:   Q8CZD5
- GeneID:   1015823
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2758
- NMPDR:   fig|221109.1.peg.2758
- HOGENOM:   HBG517865
- OMA:   AISIKSK
- BioCyc:   OIHE221109:OB2758-MONOMER
- GO:   GO:0005737
- GO:   GO:0016020
- InterPro:   IPR011055
- InterPro:   IPR001127
- TIGRFAMs:   TIGR00830

Pfam domain/function: PF00358 PTS_EIIA_1; SSF51261 Dup_hybrid_motif

EC number: 2.7.1.69

Molecular weight: Translated: 17970; Mature: 17970

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS51093 PTS_EIIA_TYPE_1

Important sites: ACT_SITE 86-86

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKNLFKIKEREPKEITVVAPITGEIIPLEEVPDPVFAQKMMGEGVAVKPANGEVISPVD
CCCCCEECCCCCCCEEEEEECCCCCEEEHHHCCCHHHHHHHHCCCEEEECCCCCEECCCC
GEVKLVFQTKHAIIVEAENNAEILIHIGLDTVNLEGEGFTAHVKDGDIVKVGDKLMSFDI
CCEEEEEEECEEEEEEECCCCEEEEEECCEEEEECCCCEEEEECCCCEEEECCCEEEEEE
ATIEEKATSSITPIIISNTDNVREVKNIKVTEVTAGENQILQVIN
EECCCCCCCCCCEEEEECCCCCHHHHCEEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MFKNLFKIKEREPKEITVVAPITGEIIPLEEVPDPVFAQKMMGEGVAVKPANGEVISPVD
CCCCCEECCCCCCCEEEEEECCCCCEEEHHHCCCHHHHHHHHCCCEEEECCCCCEECCCC
GEVKLVFQTKHAIIVEAENNAEILIHIGLDTVNLEGEGFTAHVKDGDIVKVGDKLMSFDI
CCEEEEEEECEEEEEEECCCCEEEEEECCEEEEECCCCEEEEECCCCEEEECCCEEEEEE
ATIEEKATSSITPIIISNTDNVREVKNIKVTEVTAGENQILQVIN
EECCCCCCCCCCEEEEECCCCCHHHHCEEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376