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The map label for this gene is gmuE [H]

Identifier: 23100212

GI number: 23100212

Start: 2828869

End: 2829723

Strand: Reverse

Name: gmuE [H]

Synonym: OB2757

Alternate gene names: 23100212

Gene position: 2829723-2828869 (Counterclockwise)

Preceding gene: 23100213

Following gene: 23100211

Centisome position: 77.94

GC content: 39.65

Gene sequence:

>855_bases
ATGCGAATTGGAGCAATTGAAGCAGGTGGTACAAAATTTGTTTGTGCGGTAGGTAGTCGAAATGGTGAGATTTTAGAACG
TTCCTCCTTTCCTACAACTACCCCAGATGAAACGTTAGATCAAGTAGAAGCGTTTTTTCGTGAACATCAAGTAGAACGTC
TTGGGGTTGGAGCGTTTGGTCCCATAAATCTGCGCACAGTAAGTGAACAGTATGGCACAATCGGTAATACTCCCAAAATG
GCCTGGCGGAATTTCCCGTTACTACAAAACTTAAGAAGTCGTCTAGAAATACCTGTTTTCATTGATACGGATGTGAATGT
TGCCTCATTAGGCGAGTATAATTATGGAGTAGCTAAAGATGCTGGGAGTTGTCTTTATATTACTGTTGGAACAGGAATTG
GAGCAGGTTTTGTGCAAAACGGAAACATTTTTCAAGGATACGATCATTCAGAAATGGGGCATATTCTGATTCAAAAGCAT
CCTGAAGATGATTTTGAAGGTGCTTGCCCTAGTCATAGGATTTGCCTAGAAGGATTAGCTGCAGGTCCAGCAATTGAGGC
ACGTTATGGAAAAAAAGGAATGGAATTAGCTGACCAAAATAATGTTTGGGAATTAGAAGCATATTACCTCGCACAAGCCA
TCATGAATTATTTTGTCATTCTTTCACCAGAAAAAATTATTATCGGCGGTGGTGTAATGAAACAAGACAAATTGTATCCG
ATGATTCGCAAGCAATTTATGAATCTATTAAATGATTATATCGAAGTGGAGGACGTCAATCAGCTGATTGTTGCACCAGA
ACTCAAGGATAATCAAGCAGTGAAAGGTGCCATCGCTCTAACATTACAAGATTAA

Upstream 100 bases:

>100_bases
GATAATGTAAGAGAAGTTAAAAATATAAAAGTAACAGAAGTTACAGCTGGTGAGAATCAGATTTTACAAGTGATTAACTA
GTTGAGGAGGAGATAATGCC

Downstream 100 bases:

>100_bases
TATTGAGTATATAGAAGTCAGAAAGAACTGTCGTAAATAGCGACAGTTCTTTTTTTATGAGGTTATGAGGTGAGTTAGTT
TCGATAGGTACTTGTAACAG

Product: polyketide synthase

Products: NA

Alternate protein names: Glucomannan utilization protein E [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MRIGAIEAGGTKFVCAVGSRNGEILERSSFPTTTPDETLDQVEAFFREHQVERLGVGAFGPINLRTVSEQYGTIGNTPKM
AWRNFPLLQNLRSRLEIPVFIDTDVNVASLGEYNYGVAKDAGSCLYITVGTGIGAGFVQNGNIFQGYDHSEMGHILIQKH
PEDDFEGACPSHRICLEGLAAGPAIEARYGKKGMELADQNNVWELEAYYLAQAIMNYFVILSPEKIIIGGGVMKQDKLYP
MIRKQFMNLLNDYIEVEDVNQLIVAPELKDNQAVKGAIALTLQD

Sequences:

>Translated_284_residues
MRIGAIEAGGTKFVCAVGSRNGEILERSSFPTTTPDETLDQVEAFFREHQVERLGVGAFGPINLRTVSEQYGTIGNTPKM
AWRNFPLLQNLRSRLEIPVFIDTDVNVASLGEYNYGVAKDAGSCLYITVGTGIGAGFVQNGNIFQGYDHSEMGHILIQKH
PEDDFEGACPSHRICLEGLAAGPAIEARYGKKGMELADQNNVWELEAYYLAQAIMNYFVILSPEKIIIGGGVMKQDKLYP
MIRKQFMNLLNDYIEVEDVNQLIVAPELKDNQAVKGAIALTLQD
>Mature_284_residues
MRIGAIEAGGTKFVCAVGSRNGEILERSSFPTTTPDETLDQVEAFFREHQVERLGVGAFGPINLRTVSEQYGTIGNTPKM
AWRNFPLLQNLRSRLEIPVFIDTDVNVASLGEYNYGVAKDAGSCLYITVGTGIGAGFVQNGNIFQGYDHSEMGHILIQKH
PEDDFEGACPSHRICLEGLAAGPAIEARYGKKGMELADQNNVWELEAYYLAQAIMNYFVILSPEKIIIGGGVMKQDKLYP
MIRKQFMNLLNDYIEVEDVNQLIVAPELKDNQAVKGAIALTLQD

Specific function: Seems to be involved in the degradation of glucomannan [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1786891, Length=277, Percent_Identity=26.3537906137184, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081733, Length=311, Percent_Identity=27.0096463022508, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31324; Mature: 31324

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGAIEAGGTKFVCAVGSRNGEILERSSFPTTTPDETLDQVEAFFREHQVERLGVGAFG
CEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PINLRTVSEQYGTIGNTPKMAWRNFPLLQNLRSRLEIPVFIDTDVNVASLGEYNYGVAKD
CCEEEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCC
AGSCLYITVGTGIGAGFVQNGNIFQGYDHSEMGHILIQKHPEDDFEGACPSHRICLEGLA
CCCEEEEEECCCCCCCEEECCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHC
AGPAIEARYGKKGMELADQNNVWELEAYYLAQAIMNYFVILSPEKIIIGGGVMKQDKLYP
CCCEEEHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCHHHH
MIRKQFMNLLNDYIEVEDVNQLIVAPELKDNQAVKGAIALTLQD
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRIGAIEAGGTKFVCAVGSRNGEILERSSFPTTTPDETLDQVEAFFREHQVERLGVGAFG
CEEEEEECCCCEEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PINLRTVSEQYGTIGNTPKMAWRNFPLLQNLRSRLEIPVFIDTDVNVASLGEYNYGVAKD
CCEEEEEHHHHCCCCCCCCHHHCCCHHHHHHHHHCCCEEEEECCCCCEECCCCCCCCCCC
AGSCLYITVGTGIGAGFVQNGNIFQGYDHSEMGHILIQKHPEDDFEGACPSHRICLEGLA
CCCEEEEEECCCCCCCEEECCCEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHC
AGPAIEARYGKKGMELADQNNVWELEAYYLAQAIMNYFVILSPEKIIIGGGVMKQDKLYP
CCCEEEHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHEEEECCCEEEEECCCCCCCHHHH
MIRKQFMNLLNDYIEVEDVNQLIVAPELKDNQAVKGAIALTLQD
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]