Click here to switch to the map view.

The map label for this gene is gutB [H]

Identifier: 23100207

GI number: 23100207

Start: 2822975

End: 2824009

Strand: Reverse

Name: gutB [H]

Synonym: OB2752

Alternate gene names: 23100207

Gene position: 2824009-2822975 (Counterclockwise)

Preceding gene: 23100208

Following gene: 23100206

Centisome position: 77.79

GC content: 38.16

Gene sequence:

>1035_bases
ATGAAGGCATTAGTGAAAACAGAGCTAGGGCATGGTAATCTACACATTCAAAATGTTGAAGAACCAAGCGTATCTAGTCA
TCAAGTGAAAATAAAAGTAGCTTATGCAGGTATTTGCGGTTCGGATATTCATACGTACGAAGGTCATTATAAAGTAGCTG
CTCCGGTAACTTTAGGACATGAGTTTTCCGGTCGGATTGTAGAGGTTGGTGAAGGAGTTACCGATTTTAAAGTCGGTGAT
CGTGTTACTTCAGAAACAACTTTTTCTATTTGTGGAGAATGTCGCTATTGTCAAACTGGTGATTATAATTTATGCAATCA
TCGTAAAGGGTTAGGTACGCAGCAAAATGGTGGATTTACGAATTACTTGATTGCTAGAGAAGAAAGTCTTCACCATTTGC
CCGACGAAGTGGATGATCGTTCTGCATCCATGACAGAACCATTAGCATGTACGTATCATGCTATTCAAAAAATAAATATT
AAGCCAGGAGATTTAGTCGTTGTGCTTGGACCAGGTCCAATTGGTTTGTTAGCAGCTCAGGTTGCTAAAAGCTATGGAGC
TACCGTCATGATGTCCGGACTCGATAATGACAAAATTCGTCTGGATAAAGCGAAAGAAGTTGGCATTGACTACGTTATCA
ACATTCAAGAAGAAGATATAAAGGAAAAAATTGCAGCTTTAACGGATGGTTATGGAGTGGATGTAGTATTAGAATGTTCT
GGTTCGATTCATGCTGCTAATCAAGGTCTCGACTTACTACGGAAAAAAGGAAGCTACGCACAAGTAGGAATTTTTCCAGA
CTCACATGTGAAGTTTGATATGGAAAAAGTCATTCAAAAAGAAATACATGTTGTAGGAAGTAGAAGTCAAAAATCAGCAG
ATTGGGAACCATCACTTCAGTTAATGAAAGAAAAGAAAGTAGATGCAAAAGCCTTAGTAACGCATGAATTCACTATTGAT
CAGTGGGATGAAGCATACCAAGCGATAAAAGACGGAGAGGCAATAAAAGTACTATTAAAACCATTACAAGATTAA

Upstream 100 bases:

>100_bases
TTTTTAAAAGAAACTTAGCAATTATGAAATTGATTCATCTTGTATTGAATAGGATTGGCAATCCTTTTAGGGTTATAACT
ACAGTAGGAGGCACATCGGC

Downstream 100 bases:

>100_bases
CTTCATGAGGAGGCTTACCATTATGGAAAAAAGAGAATTAGTAAATATCATTGATTATACGTTATTACATCCAACAGCAA
ATAAAGCTGACATAGAGAGC

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKALVKTELGHGNLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGHEFSGRIVEVGEGVTDFKVGD
RVTSETTFSICGECRYCQTGDYNLCNHRKGLGTQQNGGFTNYLIAREESLHHLPDEVDDRSASMTEPLACTYHAIQKINI
KPGDLVVVLGPGPIGLLAAQVAKSYGATVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECS
GSIHAANQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQLMKEKKVDAKALVTHEFTID
QWDEAYQAIKDGEAIKVLLKPLQD

Sequences:

>Translated_344_residues
MKALVKTELGHGNLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGHEFSGRIVEVGEGVTDFKVGD
RVTSETTFSICGECRYCQTGDYNLCNHRKGLGTQQNGGFTNYLIAREESLHHLPDEVDDRSASMTEPLACTYHAIQKINI
KPGDLVVVLGPGPIGLLAAQVAKSYGATVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECS
GSIHAANQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQLMKEKKVDAKALVTHEFTID
QWDEAYQAIKDGEAIKVLLKPLQD
>Mature_344_residues
MKALVKTELGHGNLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGHEFSGRIVEVGEGVTDFKVGD
RVTSETTFSICGECRYCQTGDYNLCNHRKGLGTQQNGGFTNYLIAREESLHHLPDEVDDRSASMTEPLACTYHAIQKINI
KPGDLVVVLGPGPIGLLAAQVAKSYGATVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECS
GSIHAANQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQLMKEKKVDAKALVTHEFTID
QWDEAYQAIKDGEAIKVLLKPLQD

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=342, Percent_Identity=30.7017543859649, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4501929, Length=363, Percent_Identity=28.9256198347107, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI71565154, Length=376, Percent_Identity=28.4574468085106, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI71565152, Length=375, Percent_Identity=27.4666666666667, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI4501933, Length=378, Percent_Identity=29.3650793650794, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI34577061, Length=366, Percent_Identity=28.6885245901639, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI156523966, Length=361, Percent_Identity=26.3157894736842, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI4501939, Length=355, Percent_Identity=26.1971830985915, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI71743840, Length=357, Percent_Identity=26.6106442577031, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI262073058, Length=359, Percent_Identity=26.7409470752089, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI194239674, Length=328, Percent_Identity=24.6951219512195, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI13236495, Length=328, Percent_Identity=24.6951219512195, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788075, Length=357, Percent_Identity=31.9327731092437, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1790045, Length=344, Percent_Identity=31.9767441860465, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1788073, Length=324, Percent_Identity=33.3333333333333, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI226510992, Length=321, Percent_Identity=32.0872274143302, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788407, Length=325, Percent_Identity=29.2307692307692, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787863, Length=314, Percent_Identity=32.8025477707006, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87082125, Length=355, Percent_Identity=31.2676056338028, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1790718, Length=319, Percent_Identity=28.5266457680251, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI87081918, Length=312, Percent_Identity=31.0897435897436, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1786825, Length=394, Percent_Identity=25.6345177664975, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786552, Length=381, Percent_Identity=27.8215223097113, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786518, Length=318, Percent_Identity=25.7861635220126, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082401, Length=352, Percent_Identity=25.2840909090909, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787572, Length=207, Percent_Identity=30.4347826086957, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=341, Percent_Identity=29.9120234604106, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17562878, Length=340, Percent_Identity=27.9411764705882, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17562584, Length=340, Percent_Identity=27.9411764705882, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71988145, Length=339, Percent_Identity=26.8436578171091, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17562582, Length=300, Percent_Identity=27.3333333333333, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25146526, Length=365, Percent_Identity=25.4794520547945, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71997431, Length=367, Percent_Identity=25.3405994550409, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17565904, Length=347, Percent_Identity=25.0720461095101, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=347, Percent_Identity=32.5648414985591, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322619, Length=347, Percent_Identity=32.5648414985591, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323099, Length=332, Percent_Identity=31.9277108433735, Blast_Score=153, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6319258, Length=370, Percent_Identity=30, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6319257, Length=380, Percent_Identity=27.6315789473684, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324486, Length=333, Percent_Identity=27.3273273273273, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319621, Length=309, Percent_Identity=28.8025889967638, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6323961, Length=341, Percent_Identity=26.099706744868, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323729, Length=300, Percent_Identity=26.3333333333333, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=368, Percent_Identity=25.2717391304348, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319520, Length=284, Percent_Identity=25.7042253521127, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319949, Length=323, Percent_Identity=26.9349845201238, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323980, Length=231, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137530, Length=345, Percent_Identity=29.8550724637681, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737897, Length=345, Percent_Identity=30.4347826086957, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI221457811, Length=313, Percent_Identity=28.4345047923323, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45551930, Length=313, Percent_Identity=28.4345047923323, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550770, Length=313, Percent_Identity=28.4345047923323, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17737895, Length=370, Percent_Identity=26.2162162162162, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37704; Mature: 37704

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVKTELGHGNLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGH
CCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCEECCCCEEEEEEEEECC
EFSGRIVEVGEGVTDFKVGDRVTSETTFSICGECRYCQTGDYNLCNHRKGLGTQQNGGFT
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
NYLIAREESLHHLPDEVDDRSASMTEPLACTYHAIQKINIKPGDLVVVLGPGPIGLLAAQ
EEEEEECCHHHCCCCCCCCCCCCCCCCHHHEEHHHEEECCCCCCEEEEECCCCHHHHHHH
VAKSYGATVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECS
HHHHCCCEEEEECCCCCEEEEECHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECC
GSIHAANQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQ
CCEEECCCCHHHHHCCCCEEEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCHHHH
LMKEKKVDAKALVTHEFTIDQWDEAYQAIKDGEAIKVLLKPLQD
HHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKALVKTELGHGNLHIQNVEEPSVSSHQVKIKVAYAGICGSDIHTYEGHYKVAAPVTLGH
CCCCEEECCCCCCEEEEECCCCCCCCEEEEEEEEEEECCCCCCEECCCCEEEEEEEEECC
EFSGRIVEVGEGVTDFKVGDRVTSETTFSICGECRYCQTGDYNLCNHRKGLGTQQNGGFT
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
NYLIAREESLHHLPDEVDDRSASMTEPLACTYHAIQKINIKPGDLVVVLGPGPIGLLAAQ
EEEEEECCHHHCCCCCCCCCCCCCCCCHHHEEHHHEEECCCCCCEEEEECCCCHHHHHHH
VAKSYGATVMMSGLDNDKIRLDKAKEVGIDYVINIQEEDIKEKIAALTDGYGVDVVLECS
HHHHCCCEEEEECCCCCEEEEECHHHCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEECC
GSIHAANQGLDLLRKKGSYAQVGIFPDSHVKFDMEKVIQKEIHVVGSRSQKSADWEPSLQ
CCEEECCCCHHHHHCCCCEEEEEECCCCCCEECHHHHHHHHHHHHCCCCCCCCCCCHHHH
LMKEKKVDAKALVTHEFTIDQWDEAYQAIKDGEAIKVLLKPLQD
HHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10086842; 11058132 [H]