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The map label for this gene is gatC [H]

Identifier: 23100208

GI number: 23100208

Start: 2824346

End: 2825605

Strand: Reverse

Name: gatC [H]

Synonym: OB2753

Alternate gene names: 23100208

Gene position: 2825605-2824346 (Counterclockwise)

Preceding gene: 23100209

Following gene: 23100207

Centisome position: 77.83

GC content: 39.68

Gene sequence:

>1260_bases
GTGCAAGGCTTTGTTGATTTTATTCAAGCGTTTTTAGGATTAGGTGCAACCGTAATTTTGCCAGTTGCAATTTTCTTAAT
AGGGCTATTGTTCGGTCAAAAACCAGGAAAAGCATTCCGGTCTGGTTTAACAATTGGTGTGGCATTTGTAGGTATTTTTC
TAGTAATAGACTTATTGACGTTAAACTTAGGTCCAGCCGCTCAGGGGATGGTCGATCGCTTAGGAGTGAACTTAAATGTA
ATTGATATTGGATGGCCAGCAACTTCATCTATTGCATGGGCTTCAGCAATTGCAGCATTTATTATTCCTTTGGGATTAGC
TGTAAACGTTGTCATGCTTGTTACTAAAACGACGAAAACGATGAACGTGGATATTTGGAACTTCTGGCATTATACATTTA
TGGGTGCGGTTGTATACGCTGTGTCCGATAGTATCATCCAAGGGTTAATTGCAGCGACCATTTTCCAAATCGTATGTTTG
AAAATTGCAGATTGGACGCAACCAATGGTTGAAGAATTTTATGATATGCCAGGTGTATCTGTAGCAACTGGAAGCACCAT
TTCGTATGCCCCATTTATTTTTGTTGTAAAAGGACTTCAGAAAATTCCTGGATTAAACAAATTAAATGCAGACCCAGATT
CTATTCAAAAGCGCTTTGGTGTATTTGGAGATTCGATGGTTATTGGGCTTCTACTAGGTGCAGCTATCGGGGCATTAGCT
GGATATGGTGTAGGTGAGATCATTAATATAGGTATTTCCATGGCAGCGGTAATGGTATTAATGCCACGTATGGTAAAAAT
CTTAATGGAAGGTCTTATGCCAGTATCCGAGTCCGCACGCGAATGGTTAAATAAGCACTTTGGTGATCGTGAAATCAATA
TTGGTTTGGATGCTGCAGTTTTGTTAGGACATCCATCTGTAATTGCGACAGCTTTAATTTTAACACCAGTGACTGTGTTA
CTAGCAGTTATCCTACCGGGGAATGCAGTATTGCCATTTGGTGATCTAGCGACAATACCATTTGTTATTGCATTTGTGGT
CGGTGCAGCACGAGGGAATATTATTCACTCTGTCATCGTCGGTACGATTATGATTGCTGTTTCTTTATATGTAGCGACGG
ATATCGCTCCATTATTCACAGATATGGCAACAAATGCAGAAATCAATTTACCTGAAGGATCTGCGATGGTATCAAGTATT
GATCAAGGTGGAAATATCGTAAACTGGGTCATCTGGAGATTCTTTGATCTATTCAATTAA

Upstream 100 bases:

>100_bases
AAAGAAACAAATTTTAGAAGAACTTAAAAAATAAGGTGAAATTGCTCGATTCTAACTTACCAGAGTCGAGCTTCATCTTA
AACATGGAGGGGGAGTTTCT

Downstream 100 bases:

>100_bases
TTACTTATCTGAATCAATTTCATAATTAATATATGTGATATAAAATCCGAATGTTGCGCTGTTTGATTTCCATTGCAGAC
GGACGCTTTCCGCAGGCACG

Product: PTS system galactitol-specific enzyme II C component

Products: galactitol-1-phosphate [Cytoplasm]; pyruvate [C]

Alternate protein names: EIIC-Gat; PTS system galactitol-specific EIIC component [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQGFVDFIQAFLGLGATVILPVAIFLIGLLFGQKPGKAFRSGLTIGVAFVGIFLVIDLLTLNLGPAAQGMVDRLGVNLNV
IDIGWPATSSIAWASAIAAFIIPLGLAVNVVMLVTKTTKTMNVDIWNFWHYTFMGAVVYAVSDSIIQGLIAATIFQIVCL
KIADWTQPMVEEFYDMPGVSVATGSTISYAPFIFVVKGLQKIPGLNKLNADPDSIQKRFGVFGDSMVIGLLLGAAIGALA
GYGVGEIINIGISMAAVMVLMPRMVKILMEGLMPVSESAREWLNKHFGDREINIGLDAAVLLGHPSVIATALILTPVTVL
LAVILPGNAVLPFGDLATIPFVIAFVVGAARGNIIHSVIVGTIMIAVSLYVATDIAPLFTDMATNAEINLPEGSAMVSSI
DQGGNIVNWVIWRFFDLFN

Sequences:

>Translated_419_residues
MQGFVDFIQAFLGLGATVILPVAIFLIGLLFGQKPGKAFRSGLTIGVAFVGIFLVIDLLTLNLGPAAQGMVDRLGVNLNV
IDIGWPATSSIAWASAIAAFIIPLGLAVNVVMLVTKTTKTMNVDIWNFWHYTFMGAVVYAVSDSIIQGLIAATIFQIVCL
KIADWTQPMVEEFYDMPGVSVATGSTISYAPFIFVVKGLQKIPGLNKLNADPDSIQKRFGVFGDSMVIGLLLGAAIGALA
GYGVGEIINIGISMAAVMVLMPRMVKILMEGLMPVSESAREWLNKHFGDREINIGLDAAVLLGHPSVIATALILTPVTVL
LAVILPGNAVLPFGDLATIPFVIAFVVGAARGNIIHSVIVGTIMIAVSLYVATDIAPLFTDMATNAEINLPEGSAMVSSI
DQGGNIVNWVIWRFFDLFN
>Mature_419_residues
MQGFVDFIQAFLGLGATVILPVAIFLIGLLFGQKPGKAFRSGLTIGVAFVGIFLVIDLLTLNLGPAAQGMVDRLGVNLNV
IDIGWPATSSIAWASAIAAFIIPLGLAVNVVMLVTKTTKTMNVDIWNFWHYTFMGAVVYAVSDSIIQGLIAATIFQIVCL
KIADWTQPMVEEFYDMPGVSVATGSTISYAPFIFVVKGLQKIPGLNKLNADPDSIQKRFGVFGDSMVIGLLLGAAIGALA
GYGVGEIINIGISMAAVMVLMPRMVKILMEGLMPVSESAREWLNKHFGDREINIGLDAAVLLGHPSVIATALILTPVTVL
LAVILPGNAVLPFGDLATIPFVIAFVVGAARGNIIHSVIVGTIMIAVSLYVATDIAPLFTDMATNAEINLPEGSAMVSSI
DQGGNIVNWVIWRFFDLFN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3775

COG function: function code G; Phosphotransferase system, galactitol-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788408, Length=417, Percent_Identity=41.2470023980815, Blast_Score=344, Evalue=5e-96,
Organism=Escherichia coli, GI1790758, Length=413, Percent_Identity=40.6779661016949, Blast_Score=299, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013853
- InterPro:   IPR013014
- InterPro:   IPR004703 [H]

Pfam domain/function: PF03611 EIIC-GAT [H]

EC number: NA

Molecular weight: Translated: 44495; Mature: 44495

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGFVDFIQAFLGLGATVILPVAIFLIGLLFGQKPGKAFRSGLTIGVAFVGIFLVIDLLT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
LNLGPAAQGMVDRLGVNLNVIDIGWPATSSIAWASAIAAFIIPLGLAVNVVMLVTKTTKT
HCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
MNVDIWNFWHYTFMGAVVYAVSDSIIQGLIAATIFQIVCLKIADWTQPMVEEFYDMPGVS
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VATGSTISYAPFIFVVKGLQKIPGLNKLNADPDSIQKRFGVFGDSMVIGLLLGAAIGALA
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GYGVGEIINIGISMAAVMVLMPRMVKILMEGLMPVSESAREWLNKHFGDREINIGLDAAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCHHH
LLGHPSVIATALILTPVTVLLAVILPGNAVLPFGDLATIPFVIAFVVGAARGNIIHSVIV
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
GTIMIAVSLYVATDIAPLFTDMATNAEINLPEGSAMVSSIDQGGNIVNWVIWRFFDLFN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGFVDFIQAFLGLGATVILPVAIFLIGLLFGQKPGKAFRSGLTIGVAFVGIFLVIDLLT
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHH
LNLGPAAQGMVDRLGVNLNVIDIGWPATSSIAWASAIAAFIIPLGLAVNVVMLVTKTTKT
HCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCE
MNVDIWNFWHYTFMGAVVYAVSDSIIQGLIAATIFQIVCLKIADWTQPMVEEFYDMPGVS
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCE
VATGSTISYAPFIFVVKGLQKIPGLNKLNADPDSIQKRFGVFGDSMVIGLLLGAAIGALA
EECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
GYGVGEIINIGISMAAVMVLMPRMVKILMEGLMPVSESAREWLNKHFGDREINIGLDAAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEECCCCHHH
LLGHPSVIATALILTPVTVLLAVILPGNAVLPFGDLATIPFVIAFVVGAARGNIIHSVIV
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
GTIMIAVSLYVATDIAPLFTDMATNAEINLPEGSAMVSSIDQGGNIVNWVIWRFFDLFN
HHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoenolpyruvate; galactitol [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + galactitol [Periplasm] = galactitol-1-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]