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The map label for this gene is nos [H]

Identifier: 23100146

GI number: 23100146

Start: 2762112

End: 2763221

Strand: Direct

Name: nos [H]

Synonym: OB2691

Alternate gene names: 23100146

Gene position: 2762112-2763221 (Clockwise)

Preceding gene: 23100145

Following gene: 23100147

Centisome position: 76.08

GC content: 36.49

Gene sequence:

>1110_bases
TTGACGATAGAAAAAGTTTCTCAAGAAGCGAAGTTGTTTTTAACACAATACTTTAATGAAACAGATAAACCATATGAGTA
CTTAACCCAACGAATTGCTCAAGTTAACTCTGAGATAGATCAAAAAGGATATTATACACAAACGTACGATGAGATAGTCT
ATGGCGCTAAGTTAGCATGGAGAAATTCCAACCGATGTATTGGTAGACTTTTTTGGGAACGGATAGAAGTCGCTGATGCC
CGAAAAGCTGACACTTACGAGGAAGTTTTCGACTACCTTCTCAGCCATATACAATTATCGTTCAACCATGGTAGAATCAA
GCCATATATTACTATCTTCCGTCCACAACAAGAAAAGAAAAACATCATAAAAATTTGGAATCATCAACTATTAAGGTATG
CAGGTTATCAAACGGAGGAGGGTATCATCGGAGATTCAGATTCCATCCAATTCACAAAAATTTGTGAAGGTTTAGGTTGG
AAAGGCGCACGCACAAATTTCGATATCTTACCACTCGTTGTACAAATTGGAGACAACCATCCTCAATGGATGGAAATCCC
AGATGACATAATTCCAGAAATACCTATCGAGCATCCAGATTATCCAGAAATCGCTTCGTTAAATTTAAAATGGTATGCTA
TTCCAATCATATCTGATATGCGTTTAGAGATTGGTGGACTCGATTATATTGCAGCTCCATTCAATGGATGGTATATGGGA
ACAGAAATAGGTGCAAGGAATTTTGCCGATGAGTATCGATATAATATGTTACCAAAGGTCGCTGAAAAGATGAACATAAA
TACAAAACATGAAAGTAATCTTTGGAGAGATCGAGCATTAGTAGAATTAAATGCCGCAGTTCTATATTCATTTAAAAAGC
AAGGTATTACAATTACAGACCATCACACTGCAGCAAAACAATTCGAAGTTTTCGAGAAGCATGAGGCGGCAAAAGGTCGG
GATGTCACAGGTAAGTGGACTTGGCTTATACCACCTGTCTCTCCTGCAACAACACATATATGGCATAAACCTTATACTAA
TGATATTTTAACACCTAATTTTTTTTATCAAGATAAATCTTATCAACCATTATCTTGTCCTTTTCATTAA

Upstream 100 bases:

>100_bases
ACACACCTATATAGAATGCTACTTAGGTGACAATCCTTACATCGCTTCGATAGAATAGGTATGTCGAATATACTCGAAGA
TTAATAGGAGGTACATTCCT

Downstream 100 bases:

>100_bases
TAATTTGTCCATGAGGGATAAATCTAAAGCTTTTGGAGGAATTTGTTAGAATGAACCTTTCATCAATTTTATTATGGGGA
ATGTTATCCATTTTACCAGG

Product: nitric-oxide synthase

Products: NA

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTIEKVSQEAKLFLTQYFNETDKPYEYLTQRIAQVNSEIDQKGYYTQTYDEIVYGAKLAWRNSNRCIGRLFWERIEVADA
RKADTYEEVFDYLLSHIQLSFNHGRIKPYITIFRPQQEKKNIIKIWNHQLLRYAGYQTEEGIIGDSDSIQFTKICEGLGW
KGARTNFDILPLVVQIGDNHPQWMEIPDDIIPEIPIEHPDYPEIASLNLKWYAIPIISDMRLEIGGLDYIAAPFNGWYMG
TEIGARNFADEYRYNMLPKVAEKMNINTKHESNLWRDRALVELNAAVLYSFKKQGITITDHHTAAKQFEVFEKHEAAKGR
DVTGKWTWLIPPVSPATTHIWHKPYTNDILTPNFFYQDKSYQPLSCPFH

Sequences:

>Translated_369_residues
MTIEKVSQEAKLFLTQYFNETDKPYEYLTQRIAQVNSEIDQKGYYTQTYDEIVYGAKLAWRNSNRCIGRLFWERIEVADA
RKADTYEEVFDYLLSHIQLSFNHGRIKPYITIFRPQQEKKNIIKIWNHQLLRYAGYQTEEGIIGDSDSIQFTKICEGLGW
KGARTNFDILPLVVQIGDNHPQWMEIPDDIIPEIPIEHPDYPEIASLNLKWYAIPIISDMRLEIGGLDYIAAPFNGWYMG
TEIGARNFADEYRYNMLPKVAEKMNINTKHESNLWRDRALVELNAAVLYSFKKQGITITDHHTAAKQFEVFEKHEAAKGR
DVTGKWTWLIPPVSPATTHIWHKPYTNDILTPNFFYQDKSYQPLSCPFH
>Mature_368_residues
TIEKVSQEAKLFLTQYFNETDKPYEYLTQRIAQVNSEIDQKGYYTQTYDEIVYGAKLAWRNSNRCIGRLFWERIEVADAR
KADTYEEVFDYLLSHIQLSFNHGRIKPYITIFRPQQEKKNIIKIWNHQLLRYAGYQTEEGIIGDSDSIQFTKICEGLGWK
GARTNFDILPLVVQIGDNHPQWMEIPDDIIPEIPIEHPDYPEIASLNLKWYAIPIISDMRLEIGGLDYIAAPFNGWYMGT
EIGARNFADEYRYNMLPKVAEKMNINTKHESNLWRDRALVELNAAVLYSFKKQGITITDHHTAAKQFEVFEKHEAAKGRD
VTGKWTWLIPPVSPATTHIWHKPYTNDILTPNFFYQDKSYQPLSCPFH

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=350, Percent_Identity=43.1428571428571, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI24041029, Length=353, Percent_Identity=42.4929178470255, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI231571356, Length=357, Percent_Identity=40.0560224089636, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI40254422, Length=362, Percent_Identity=39.5027624309392, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI231571314, Length=357, Percent_Identity=40.0560224089636, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI231571329, Length=357, Percent_Identity=40.0560224089636, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI78706882, Length=357, Percent_Identity=40.6162464985994, Blast_Score=312, Evalue=2e-85,
Organism=Drosophila melanogaster, GI78706868, Length=357, Percent_Identity=40.6162464985994, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24583543, Length=359, Percent_Identity=40.3899721448468, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI78706876, Length=359, Percent_Identity=40.3899721448468, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI78706866, Length=357, Percent_Identity=40.6162464985994, Blast_Score=311, Evalue=3e-85,
Organism=Drosophila melanogaster, GI78706870, Length=357, Percent_Identity=40.6162464985994, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI78706878, Length=357, Percent_Identity=40.6162464985994, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI78706880, Length=357, Percent_Identity=40.6162464985994, Blast_Score=311, Evalue=4e-85,
Organism=Drosophila melanogaster, GI78706872, Length=150, Percent_Identity=40.6666666666667, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: NA

Molecular weight: Translated: 43186; Mature: 43055

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIEKVSQEAKLFLTQYFNETDKPYEYLTQRIAQVNSEIDQKGYYTQTYDEIVYGAKLAW
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEE
RNSNRCIGRLFWERIEVADARKADTYEEVFDYLLSHIQLSFNHGRIKPYITIFRPQQEKK
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEECCCCCCEEEEEECCCHHHH
NIIKIWNHQLLRYAGYQTEEGIIGDSDSIQFTKICEGLGWKGARTNFDILPLVVQIGDNH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
PQWMEIPDDIIPEIPIEHPDYPEIASLNLKWYAIPIISDMRLEIGGLDYIAAPFNGWYMG
CCCCCCCHHHCCCCCCCCCCCCCCEEECEEEEEEEEEECCEEEECCCEEEECCCCCEEEE
TEIGARNFADEYRYNMLPKVAEKMNINTKHESNLWRDRALVELNAAVLYSFKKQGITITD
CHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHEEECHHHHHHHHHCCEEEEC
HHTAAKQFEVFEKHEAAKGRDVTGKWTWLIPPVSPATTHIWHKPYTNDILTPNFFYQDKS
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCEEECCCC
YQPLSCPFH
CCCCCCCCC
>Mature Secondary Structure 
TIEKVSQEAKLFLTQYFNETDKPYEYLTQRIAQVNSEIDQKGYYTQTYDEIVYGAKLAW
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEE
RNSNRCIGRLFWERIEVADARKADTYEEVFDYLLSHIQLSFNHGRIKPYITIFRPQQEKK
CCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEECCCCCCEEEEEECCCHHHH
NIIKIWNHQLLRYAGYQTEEGIIGDSDSIQFTKICEGLGWKGARTNFDILPLVVQIGDNH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
PQWMEIPDDIIPEIPIEHPDYPEIASLNLKWYAIPIISDMRLEIGGLDYIAAPFNGWYMG
CCCCCCCHHHCCCCCCCCCCCCCCEEECEEEEEEEEEECCEEEECCCEEEECCCCCEEEE
TEIGARNFADEYRYNMLPKVAEKMNINTKHESNLWRDRALVELNAAVLYSFKKQGITITD
CHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHEEECHHHHHHHHHCCEEEEC
HHTAAKQFEVFEKHEAAKGRDVTGKWTWLIPPVSPATTHIWHKPYTNDILTPNFFYQDKS
CCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEECCCCCCCCCCCCEEECCCC
YQPLSCPFH
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]