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The map label for this gene is alsT [H]

Identifier: 23100145

GI number: 23100145

Start: 2760107

End: 2761528

Strand: Direct

Name: alsT [H]

Synonym: OB2690

Alternate gene names: 23100145

Gene position: 2760107-2761528 (Clockwise)

Preceding gene: 23100144

Following gene: 23100146

Centisome position: 76.02

GC content: 36.71

Gene sequence:

>1422_bases
ATGTTGGAAATCATTCAATCAATTATAGATCATGTAAATACCTTTTTATGGTCATATGTATTAATCATCGTATTAGTAGG
TTTAGGATTATACTATACCGTTAAAACAAATTTTGTTCAGTTTCGTTTATTGCCGGAAATGTTTCGAGTTATTGGAGATA
AACGTTCGTTTGATGCTTCAGGTAAAAAAGGAACGAGTTCCTTTCAAGCATTTGCCATAAGTGCAGCATCCCGTGTTGGA
ACAGGAAATATGGCTGGCGTTGCGTCGGCTGTTGCTTTAGGTGGTCCGGGTGCGCTATTTTGGATGTGGCTTATCGCCCT
ATTAGGAGCTGCTTCTGGTTTTGTGGAAAGTACACTAGCACAACTTTATAAAGAAAAGGACTCGAATCAATATCGAGGCG
GCCCTGCCTATTATATCGAAAAAGGTATGAAAAAACGTTGGCTCGGAATTGTATTTGCCATTACGATAACATTTACATAT
GGGCTCGTATTCAATTCCGTACAATCCAATACAATCAGTCTAGCATTCGAAGGGCAATTTCAAATTGATCCTGTTTGGAT
CGCTATTGTATTAGCCACACTCGTTGCTGTGATTATATTTGGTGGACTAAAGAGTATAGCTAATGTATCACAAATTATTG
TACCTTTTATGGCAATAATCTATATCGTACTTGCTTTAATCATTCTAATAATGAACATTCCTGCTATTCCAGATATGTTT
GCACTAATTTTTGCAAATGCATTTGGAATAGAACAAGTAGCAGGTGGTGGATTTGGTGCAGCAATTATGATGGGAATTAA
ACGTGGACTATTCTCTAATGAAGCTGGTATGGGTGCTGCTCCTAATGCTGCTGCAACTGCTGAAGTCAGTCACCCAGTAA
AACAAGGATTAGTTCAAGCACTAGGTGTATTTTTTGATACTATTCTAGTATGTACAGCAACTGGATTTGTTATCTTGACT
GCAGGTGGTTTTGCAGGTAGTGAAGATGACGGTATACAAATAACACAATCTGCATTTACACAGTCCCTTGGCGATTGGGC
AGGAATATTTATTGCAATAGCGATATTCTTATTTGCATTTAGTTCTATCCTTGGTAACTATTACTATGGGGAAAATAATA
TTGCATATATACGCGATACGAAGATCGGTTTATTTATTTATCGAATTGCAGTTCTAGCAATGGTTATTTTTGGAACGCTG
GCTTCATTTGATATGGTATGGTCGTTAGCAGATGTAACGATGGCATTGCTTGCATTAATTAACTTGTATGCCATTACTAT
CCTATTTAATAAAGCAAATATACTTCTCAAAGACTATGTGAAACAGCGGAAACAAGGAAAAGATCCTGTATTTTATAAAG
ATACGTTAGATGATCAAACTGGTATTGAATGTTGGGATCGAGAATTCCACAAAAATCAATAA

Upstream 100 bases:

>100_bases
GATGTTGTTAACCTATTCCTTCATTTATTACGAATCATATCTTATGTTTTCAAAAATAGAGGTTAACTACGCTTGTTATT
ATTTATAGAAAGGAAGATTA

Downstream 100 bases:

>100_bases
TATGTAACGAAAAGCATCGCTTCATGCGATGCTTTTTTTGTTATCTAGGAAATTATAAAATTTTGGGGCTGTGGCTAAAC
AACTAAGTTATTTAGCCACG

Product: sodium:alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MLEIIQSIIDHVNTFLWSYVLIIVLVGLGLYYTVKTNFVQFRLLPEMFRVIGDKRSFDASGKKGTSSFQAFAISAASRVG
TGNMAGVASAVALGGPGALFWMWLIALLGAASGFVESTLAQLYKEKDSNQYRGGPAYYIEKGMKKRWLGIVFAITITFTY
GLVFNSVQSNTISLAFEGQFQIDPVWIAIVLATLVAVIIFGGLKSIANVSQIIVPFMAIIYIVLALIILIMNIPAIPDMF
ALIFANAFGIEQVAGGGFGAAIMMGIKRGLFSNEAGMGAAPNAAATAEVSHPVKQGLVQALGVFFDTILVCTATGFVILT
AGGFAGSEDDGIQITQSAFTQSLGDWAGIFIAIAIFLFAFSSILGNYYYGENNIAYIRDTKIGLFIYRIAVLAMVIFGTL
ASFDMVWSLADVTMALLALINLYAITILFNKANILLKDYVKQRKQGKDPVFYKDTLDDQTGIECWDREFHKNQ

Sequences:

>Translated_473_residues
MLEIIQSIIDHVNTFLWSYVLIIVLVGLGLYYTVKTNFVQFRLLPEMFRVIGDKRSFDASGKKGTSSFQAFAISAASRVG
TGNMAGVASAVALGGPGALFWMWLIALLGAASGFVESTLAQLYKEKDSNQYRGGPAYYIEKGMKKRWLGIVFAITITFTY
GLVFNSVQSNTISLAFEGQFQIDPVWIAIVLATLVAVIIFGGLKSIANVSQIIVPFMAIIYIVLALIILIMNIPAIPDMF
ALIFANAFGIEQVAGGGFGAAIMMGIKRGLFSNEAGMGAAPNAAATAEVSHPVKQGLVQALGVFFDTILVCTATGFVILT
AGGFAGSEDDGIQITQSAFTQSLGDWAGIFIAIAIFLFAFSSILGNYYYGENNIAYIRDTKIGLFIYRIAVLAMVIFGTL
ASFDMVWSLADVTMALLALINLYAITILFNKANILLKDYVKQRKQGKDPVFYKDTLDDQTGIECWDREFHKNQ
>Mature_473_residues
MLEIIQSIIDHVNTFLWSYVLIIVLVGLGLYYTVKTNFVQFRLLPEMFRVIGDKRSFDASGKKGTSSFQAFAISAASRVG
TGNMAGVASAVALGGPGALFWMWLIALLGAASGFVESTLAQLYKEKDSNQYRGGPAYYIEKGMKKRWLGIVFAITITFTY
GLVFNSVQSNTISLAFEGQFQIDPVWIAIVLATLVAVIIFGGLKSIANVSQIIVPFMAIIYIVLALIILIMNIPAIPDMF
ALIFANAFGIEQVAGGGFGAAIMMGIKRGLFSNEAGMGAAPNAAATAEVSHPVKQGLVQALGVFFDTILVCTATGFVILT
AGGFAGSEDDGIQITQSAFTQSLGDWAGIFIAIAIFLFAFSSILGNYYYGENNIAYIRDTKIGLFIYRIAVLAMVIFGTL
ASFDMVWSLADVTMALLALINLYAITILFNKANILLKDYVKQRKQGKDPVFYKDTLDDQTGIECWDREFHKNQ

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=468, Percent_Identity=42.0940170940171, Blast_Score=351, Evalue=5e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51382; Mature: 51382

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00217 SUGAR_TRANSPORT_2 ; PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIIQSIIDHVNTFLWSYVLIIVLVGLGLYYTVKTNFVQFRLLPEMFRVIGDKRSFDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
GKKGTSSFQAFAISAASRVGTGNMAGVASAVALGGPGALFWMWLIALLGAASGFVESTLA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHH
QLYKEKDSNQYRGGPAYYIEKGMKKRWLGIVFAITITFTYGLVFNSVQSNTISLAFEGQF
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
QIDPVWIAIVLATLVAVIIFGGLKSIANVSQIIVPFMAIIYIVLALIILIMNIPAIPDMF
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ALIFANAFGIEQVAGGGFGAAIMMGIKRGLFSNEAGMGAAPNAAATAEVSHPVKQGLVQA
HHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVFFDTILVCTATGFVILTAGGFAGSEDDGIQITQSAFTQSLGDWAGIFIAIAIFLFAF
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SSILGNYYYGENNIAYIRDTKIGLFIYRIAVLAMVIFGTLASFDMVWSLADVTMALLALI
HHHHCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLYAITILFNKANILLKDYVKQRKQGKDPVFYKDTLDDQTGIECWDREFHKNQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MLEIIQSIIDHVNTFLWSYVLIIVLVGLGLYYTVKTNFVQFRLLPEMFRVIGDKRSFDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
GKKGTSSFQAFAISAASRVGTGNMAGVASAVALGGPGALFWMWLIALLGAASGFVESTLA
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHH
QLYKEKDSNQYRGGPAYYIEKGMKKRWLGIVFAITITFTYGLVFNSVQSNTISLAFEGQF
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCE
QIDPVWIAIVLATLVAVIIFGGLKSIANVSQIIVPFMAIIYIVLALIILIMNIPAIPDMF
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ALIFANAFGIEQVAGGGFGAAIMMGIKRGLFSNEAGMGAAPNAAATAEVSHPVKQGLVQA
HHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LGVFFDTILVCTATGFVILTAGGFAGSEDDGIQITQSAFTQSLGDWAGIFIAIAIFLFAF
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
SSILGNYYYGENNIAYIRDTKIGLFIYRIAVLAMVIFGTLASFDMVWSLADVTMALLALI
HHHHCCEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLYAITILFNKANILLKDYVKQRKQGKDPVFYKDTLDDQTGIECWDREFHKNQ
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]