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The map label for this gene is ilvK [H]

Identifier: 23100083

GI number: 23100083

Start: 2694876

End: 2695970

Strand: Direct

Name: ilvK [H]

Synonym: OB2628

Alternate gene names: 23100083

Gene position: 2694876-2695970 (Clockwise)

Preceding gene: 23100081

Following gene: 28563941

Centisome position: 74.23

GC content: 37.81

Gene sequence:

>1095_bases
ATGGAAGAACAAACAATTCGCGTACAACGAAGTGAAACAAAGAAAGAAAAACCTCAGTCTGATCAGTTGATTTTCGGAAG
ATCTTTTACGGATCATATGTTTGTCATGGATTACTCTGATCCTTTGGGATGGCATAATGCGAGTATTGTACCATATCAAC
CATTGTCGATTGATCCATCTTCAATGATTTTCCATTATGGTCAATCTGTTTTTGAAGGATTAAAGGCATTTCGTACTGAG
AGCGGAGATATTCAATTATTCCGTGCGGAAAAAAACCTTGAGCGTTTAAATCGTTCAAATGACCGTCTATGTATTCCGCA
AATCGATGAGGAGTTCACACTAAAAGCAATTAAACAGTTAATTTCCATCGAAAAAGATTGGGTACCAAGTGCAGAAGGAA
CATCCCTTTATATTCGTCCTTTTATTATTTCAACAGAGGCTTATATTGGGGTCGCACCATCGATGAATTATAAGTTGATC
GTCATTTTATCTCCAGTAGGTGCTTACTACAAAGAAGGAATAAACCCAGTCAAAATTGCTGTTGAAGATCACTATGTACG
AACGGTGAAAGGTGGTACTGGTGAAGCAAAAACTGGTGGAAACTATGCATCAAGTCTAAAAGCACAAGAAATTGTAGCGA
AACAAGGATTTGCACAAGTGCTTTGGTTAGATGGTGTCGAGAAAAAGTATATTGAAGAAGTCGGCAGTATGAATGTCTTC
TTCAAAATTAATGGAGAAGTTGTTACTCCTCAATTAAACGGAAGTATTCTTTCAGGAGTAACTCGTAGCTCTGTCATTGA
ATTATTAAAACATTGGGGTGTACCTGTATCTGAAAAGCGCATTTCCATTCAGGATTTATACCAAGCTTATTTAGATGGTG
AGCTAGAAGAAGCTTTTGGATCCGGTACAGCTGCTGTAATTTCTCCAATTGGTCAACTTACTTGGGAAGGCAAAGATATG
ATCGTCAACAATGGGGAAACTGGTGAACTCTCTAAACGCATATACGATACAATTACTGGCATTCAATATGGTCATTTAGA
TGATCCATTCCAATGGATTCAAGGGATTGAAGCAAAGAGTCCCGTAAAACATTGA

Upstream 100 bases:

>100_bases
TACTAATTTAACATAATTAATTTAAAATTTAGGCTTGATAAAATCTTTTTTATGTCTTATGATAAATAGAAATTTACCAA
CAGCAAGGGAGACGTTTGAT

Downstream 100 bases:

>100_bases
AAAGACCCTGCCAACTAAATATATGAAAAATCCCCCTTATAATCTATCAAAAGGGGGATTTTTATTAGTTAATACTATAT
TGGTGACACTTATCATATAG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MEEQTIRVQRSETKKEKPQSDQLIFGRSFTDHMFVMDYSDPLGWHNASIVPYQPLSIDPSSMIFHYGQSVFEGLKAFRTE
SGDIQLFRAEKNLERLNRSNDRLCIPQIDEEFTLKAIKQLISIEKDWVPSAEGTSLYIRPFIISTEAYIGVAPSMNYKLI
VILSPVGAYYKEGINPVKIAVEDHYVRTVKGGTGEAKTGGNYASSLKAQEIVAKQGFAQVLWLDGVEKKYIEEVGSMNVF
FKINGEVVTPQLNGSILSGVTRSSVIELLKHWGVPVSEKRISIQDLYQAYLDGELEEAFGSGTAAVISPIGQLTWEGKDM
IVNNGETGELSKRIYDTITGIQYGHLDDPFQWIQGIEAKSPVKH

Sequences:

>Translated_364_residues
MEEQTIRVQRSETKKEKPQSDQLIFGRSFTDHMFVMDYSDPLGWHNASIVPYQPLSIDPSSMIFHYGQSVFEGLKAFRTE
SGDIQLFRAEKNLERLNRSNDRLCIPQIDEEFTLKAIKQLISIEKDWVPSAEGTSLYIRPFIISTEAYIGVAPSMNYKLI
VILSPVGAYYKEGINPVKIAVEDHYVRTVKGGTGEAKTGGNYASSLKAQEIVAKQGFAQVLWLDGVEKKYIEEVGSMNVF
FKINGEVVTPQLNGSILSGVTRSSVIELLKHWGVPVSEKRISIQDLYQAYLDGELEEAFGSGTAAVISPIGQLTWEGKDM
IVNNGETGELSKRIYDTITGIQYGHLDDPFQWIQGIEAKSPVKH
>Mature_364_residues
MEEQTIRVQRSETKKEKPQSDQLIFGRSFTDHMFVMDYSDPLGWHNASIVPYQPLSIDPSSMIFHYGQSVFEGLKAFRTE
SGDIQLFRAEKNLERLNRSNDRLCIPQIDEEFTLKAIKQLISIEKDWVPSAEGTSLYIRPFIISTEAYIGVAPSMNYKLI
VILSPVGAYYKEGINPVKIAVEDHYVRTVKGGTGEAKTGGNYASSLKAQEIVAKQGFAQVLWLDGVEKKYIEEVGSMNVF
FKINGEVVTPQLNGSILSGVTRSSVIELLKHWGVPVSEKRISIQDLYQAYLDGELEEAFGSGTAAVISPIGQLTWEGKDM
IVNNGETGELSKRIYDTITGIQYGHLDDPFQWIQGIEAKSPVKH

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=339, Percent_Identity=43.6578171091445, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI296010906, Length=339, Percent_Identity=43.6578171091445, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI38176287, Length=339, Percent_Identity=43.6578171091445, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI50658084, Length=348, Percent_Identity=38.5057471264368, Blast_Score=259, Evalue=4e-69,
Organism=Homo sapiens, GI296010902, Length=353, Percent_Identity=38.2436260623229, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI296010900, Length=339, Percent_Identity=39.2330383480826, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI258614015, Length=283, Percent_Identity=39.9293286219081, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI48994963, Length=317, Percent_Identity=30.2839116719243, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17568601, Length=347, Percent_Identity=35.7348703170029, Blast_Score=235, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17565728, Length=358, Percent_Identity=35.195530726257, Blast_Score=201, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6322002, Length=360, Percent_Identity=40.8333333333333, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322608, Length=346, Percent_Identity=42.1965317919075, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24641779, Length=356, Percent_Identity=38.4831460674157, Blast_Score=251, Evalue=6e-67,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 40737; Mature: 40737

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEQTIRVQRSETKKEKPQSDQLIFGRSFTDHMFVMDYSDPLGWHNASIVPYQPLSIDPS
CCCCEEEEEHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
SMIFHYGQSVFEGLKAFRTESGDIQLFRAEKNLERLNRSNDRLCIPQIDEEFTLKAIKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
ISIEKDWVPSAEGTSLYIRPFIISTEAYIGVAPSMNYKLIVILSPVGAYYKEGINPVKIA
HHHHHCCCCCCCCCEEEEEEEEEECCCEEEECCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDHYVRTVKGGTGEAKTGGNYASSLKAQEIVAKQGFAQVLWLDGVEKKYIEEVGSMNVF
EECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCEEEE
FKINGEVVTPQLNGSILSGVTRSSVIELLKHWGVPVSEKRISIQDLYQAYLDGELEEAFG
EEECCEEEECCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHC
SGTAAVISPIGQLTWEGKDMIVNNGETGELSKRIYDTITGIQYGHLDDPFQWIQGIEAKS
CCCHHHHHHHHHHEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PVKH
CCCC
>Mature Secondary Structure
MEEQTIRVQRSETKKEKPQSDQLIFGRSFTDHMFVMDYSDPLGWHNASIVPYQPLSIDPS
CCCCEEEEEHHHHHHCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHH
SMIFHYGQSVFEGLKAFRTESGDIQLFRAEKNLERLNRSNDRLCIPQIDEEFTLKAIKQL
HHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCEEECCCCCHHHHHHHHHHH
ISIEKDWVPSAEGTSLYIRPFIISTEAYIGVAPSMNYKLIVILSPVGAYYKEGINPVKIA
HHHHHCCCCCCCCCEEEEEEEEEECCCEEEECCCCCEEEEEEECCCCHHHHCCCCEEEEE
VEDHYVRTVKGGTGEAKTGGNYASSLKAQEIVAKQGFAQVLWLDGVEKKYIEEVGSMNVF
EECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCEEEE
FKINGEVVTPQLNGSILSGVTRSSVIELLKHWGVPVSEKRISIQDLYQAYLDGELEEAFG
EEECCEEEECCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHC
SGTAAVISPIGQLTWEGKDMIVNNGETGELSKRIYDTITGIQYGHLDDPFQWIQGIEAKS
CCCHHHHHHHHHHEECCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCC
PVKH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]