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The map label for this gene is ydfA [H]

Identifier: 23099879

GI number: 23099879

Start: 2477017

End: 2478315

Strand: Reverse

Name: ydfA [H]

Synonym: OB2424

Alternate gene names: 23099879

Gene position: 2478315-2477017 (Counterclockwise)

Preceding gene: 23099880

Following gene: 23099878

Centisome position: 68.26

GC content: 35.33

Gene sequence:

>1299_bases
TTGTCATCAGTGTTTATTACATCAAGTATATTTTTACTTACGCTCATATTAGTGATATGGCAACCAAAGAATTTAAATAT
CGGCTGGTCAGCATGTGGGGGAGCAATTGTTGCTTTACTTTTTGGTGTTGTCAGCATTAGTGATGTCTGGGATGTAACGG
GAATTGTTTGGAATGCAACTCTTACATTTATTGCAATCATTCTTATATCATTAATATTGGATGAAATTGGGTTTTTCGAA
TGGTCTGCGCTTCATATGTCACGTCTTGCAAAAGGCAATGGAATTCTAATGTTTATTTATGTTTGTGTGCTTGGGTCAAT
TGTTGCCGCATTTTTTGCCAATGATGGGGCAGCACTAATCTTAACACCAATTGTATTAGCAATGGTACGATCCTTAGACT
TTGATGAAAAAATAGTTTTCCCATTTATCATTGCAAGTGGATTTATTGCAGATACAACTTCACTTCCTTTAATCGTAAGT
AACTTAGTAAATATTGTTTCAGCGGACTTCTTTGGAATTGGATTTGTGGAATATGCGACACATATGATTGTTCCAACAAT
TGTTGCACTTGGATCTAGTATAATTGTGCTTTTCGTTTATTTTCGAAAAAGAATTCCAAGAACATACGATTATTCCAAAT
TAAAAGAACCGCATGAGGCGATTAAAGATCATCGTATGTTTCGCTTATCGTGGTTTGTATTAGGTTTGTTATTAGTCGGA
TATTTAGTTAGTGAGTTTGTTGGTATTCCAGTATCCTTTATAGCTGGTGTGATTGCTATTATATTTTTAATTGTTGCGAA
GAATAGTAAGGCTGTTAATACGATGACGGTTGTAAAAGGCGCTCCATGGAATATTGTCTTTTTCTCCATCGGTATGTATG
TGGTAGTTTATGGATTGCGTAATGTTGGACTAACGGATATTTTGGCTGATATTATACAAGTTGTTGCAGATCAAGGAATG
TTTGCAGCAACCATGGGAATGGGCTTCATTGCTGCATTTTTATCTTCCATAATGAATAATATGCCAACAGTGATGATTGA
TGCTCTTGCAATTGCCGAAACTTCTACAAGTCAGATGGTACGCGAGGCTTTAATCTATGCTAATGTTGTTGGTGCAGACT
TAGGGCCAAAGATTACACCAATTGGATCTTTAGCAACATTATTATGGCTTCACGTCCTAACTCAAAAAGGAGTTAAAATA
TCGTGGGGTACTTACTTTAAAACCGGAATTGTAATAACAATACCTGTCTTATTTATGACTCTTGCAGGATTGTTTATGTG
GTTAATGATTATAAATTAA

Upstream 100 bases:

>100_bases
TTGGTTCAACAGATTCTCAATACTATTAATCCAGAAGATACAAAAGTAAAAAGTATATTGTTAAAAGAAAAACCTATGGA
ATGTTAGGGAGGTATTTAGA

Downstream 100 bases:

>100_bases
AGGAGTTTTTAATATGTCTAAAAAAATTATATATTTCTTATGTACTGGAAATTCATGTCGCAGTCAAATGGCAGAAGGAT
GGGGAAAGAAAATCCTAGGT

Product: arsenic efflux pump

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSSVFITSSIFLLTLILVIWQPKNLNIGWSACGGAIVALLFGVVSISDVWDVTGIVWNATLTFIAIILISLILDEIGFFE
WSALHMSRLAKGNGILMFIYVCVLGSIVAAFFANDGAALILTPIVLAMVRSLDFDEKIVFPFIIASGFIADTTSLPLIVS
NLVNIVSADFFGIGFVEYATHMIVPTIVALGSSIIVLFVYFRKRIPRTYDYSKLKEPHEAIKDHRMFRLSWFVLGLLLVG
YLVSEFVGIPVSFIAGVIAIIFLIVAKNSKAVNTMTVVKGAPWNIVFFSIGMYVVVYGLRNVGLTDILADIIQVVADQGM
FAATMGMGFIAAFLSSIMNNMPTVMIDALAIAETSTSQMVREALIYANVVGADLGPKITPIGSLATLLWLHVLTQKGVKI
SWGTYFKTGIVITIPVLFMTLAGLFMWLMIIN

Sequences:

>Translated_432_residues
MSSVFITSSIFLLTLILVIWQPKNLNIGWSACGGAIVALLFGVVSISDVWDVTGIVWNATLTFIAIILISLILDEIGFFE
WSALHMSRLAKGNGILMFIYVCVLGSIVAAFFANDGAALILTPIVLAMVRSLDFDEKIVFPFIIASGFIADTTSLPLIVS
NLVNIVSADFFGIGFVEYATHMIVPTIVALGSSIIVLFVYFRKRIPRTYDYSKLKEPHEAIKDHRMFRLSWFVLGLLLVG
YLVSEFVGIPVSFIAGVIAIIFLIVAKNSKAVNTMTVVKGAPWNIVFFSIGMYVVVYGLRNVGLTDILADIIQVVADQGM
FAATMGMGFIAAFLSSIMNNMPTVMIDALAIAETSTSQMVREALIYANVVGADLGPKITPIGSLATLLWLHVLTQKGVKI
SWGTYFKTGIVITIPVLFMTLAGLFMWLMIIN
>Mature_431_residues
SSVFITSSIFLLTLILVIWQPKNLNIGWSACGGAIVALLFGVVSISDVWDVTGIVWNATLTFIAIILISLILDEIGFFEW
SALHMSRLAKGNGILMFIYVCVLGSIVAAFFANDGAALILTPIVLAMVRSLDFDEKIVFPFIIASGFIADTTSLPLIVSN
LVNIVSADFFGIGFVEYATHMIVPTIVALGSSIIVLFVYFRKRIPRTYDYSKLKEPHEAIKDHRMFRLSWFVLGLLLVGY
LVSEFVGIPVSFIAGVIAIIFLIVAKNSKAVNTMTVVKGAPWNIVFFSIGMYVVVYGLRNVGLTDILADIIQVVADQGMF
AATMGMGFIAAFLSSIMNNMPTVMIDALAIAETSTSQMVREALIYANVVGADLGPKITPIGSLATLLWLHVLTQKGVKIS
WGTYFKTGIVITIPVLFMTLAGLFMWLMIIN

Specific function: Involved In Arsenical Resistance. Thought To Form The Channel Of An Arsenite Pump (By Similarity). [C]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=421, Percent_Identity=60.8076009501188, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 47126; Mature: 46995

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCC
>Mature Secondary Structure 
SSVFITSSIFLLTLILVIWQPKNLNIGWSACGGAIVALLFGVVSISDVWDVTGIVWNAT
CCEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTFIAIILISLILDEIGFFEWSALHMSRLAKGNGILMFIYVCVLGSIVAAFFANDGAALI
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
LTPIVLAMVRSLDFDEKIVFPFIIASGFIADTTSLPLIVSNLVNIVSADFFGIGFVEYAT
HHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HMIVPTIVALGSSIIVLFVYFRKRIPRTYDYSKLKEPHEAIKDHRMFRLSWFVLGLLLVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YLVSEFVGIPVSFIAGVIAIIFLIVAKNSKAVNTMTVVKGAPWNIVFFSIGMYVVVYGLR
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
NVGLTDILADIIQVVADQGMFAATMGMGFIAAFLSSIMNNMPTVMIDALAIAETSTSQMV
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHH
REALIYANVVGADLGPKITPIGSLATLLWLHVLTQKGVKISWGTYFKTGIVITIPVLFMT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHCCHHHHHHHHHHH
LAGLFMWLMIIN
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]