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The map label for this gene is mls [H]

Identifier: 23099860

GI number: 23099860

Start: 2458436

End: 2460031

Strand: Reverse

Name: mls [H]

Synonym: OB2405

Alternate gene names: 23099860

Gene position: 2460031-2458436 (Counterclockwise)

Preceding gene: 28563937

Following gene: 23099859

Centisome position: 67.76

GC content: 36.09

Gene sequence:

>1596_bases
ATGAAGGATGCCACTATACTTCCAGATCATAAAAAAGCAAAATACGAAGATATTCTTAACACTGAAGCAAGAAAGTTTTT
GTTGCGATTGCACCACCATTTTAATCAAAGAAGAAAAGAACTACTTATTGAAAGAAAAGAAAAACAAAAAAGAATGTCTG
AAGGAGAAAAGTTACATTTTCTTTCCTTAACCAGACAAGTACGAGAAGCAGATTGGGAAATTGATCCAGTACCAACAGAT
TTAAAGGATAGAAGAGTCGAAATCACCGGACCTGTGGATAGAAAAATGATCATTAATGCTTTGAATTCAGGTGCCAATAC
TTTTATGGCTGATTTCGAAGATGCTAGCTCTCCAACTTGGGAGAATATGATGGAAGGACAACGAAATTTAAAAGATGCAA
TTCGAAAAGACATTGATTTTTTGGATAACAAAGGAAAACAATATCGTTTGAATGATAAAACGGCTGTAATCATTGTTCGA
CCTCGAGGATGGCACTTAGAAGAGCACCACATTACAGTTGAAAATGAGCCTATGTCTGCTAGTTTAGTAGATTTTGGATT
GTATTTTTTCCATAATGCAAAAGAGTTATTACAAAGCGGATCTGGACCATACTTTTATTTGCCAAAAATAGAAAGTTATT
TAGAGGCACGTCTTTGGAATGATGTATTTACCTTTGCCCAAAATGAAATTGGCATTCCTCAGGGAACAATCAAAGCAACT
GTACTCATTGAAACAATTACAGCTGCATTTGAAATGGATGAGATTTTATACGAGCTTCGTCATCATTCAGCAGGATTGAA
TTGCGGTAGATGGGATTATATTTTCAGTTTCATAAAAAAGAATCAACATAACCCTGCTATGTTATTACCTGATCGCTCTT
TAGTAACGATGGAAACCCATTTTATGCGCTCCTACTCATTATTAGCAATTCAGACATGTCATCGAAGAAATGCACATGCT
ATTGGAGGGATGGCTGCTCAAATTCCCGTGAAAAACAATGAAGAATTAAATCGTCAAGCATTTGACAAAGTAAAAAAAGA
TAAAGAACGGGAAGTAAATGATGGACACGATGGAACGTGGATAGCACATCCAGGTATGGTTCAACTAGTAAAGGATGTTT
TTGATCAAGGCATGCCTGCTGCTAACCAGATTAATAGAAAAAGGGAAGATATACACGTTACAGCAGATGATCTATTGAAG
TTACCGACTGGGCCAATAACAATGGAAGGTTTGCGTACAAATATAAAAGTCGGAATTCACTATGTAGCAGCCTGGCTAGC
AGGCAGAGGGGCAGTTCCAATTAATAATCTAATGGAAGATGCAGCAACAGCAGAAATCTCGAGAGCACAGGTATGGCAGT
GGATTCAAAATAAAGCAATGATGGATAATGAACAGCAAGTCACTTTTGAGGTTGTTAAGGATATAGTAGATGAAGTTTTA
GTTCAATTTGAAGAAAGCTTAGGTATTGAAGGGATAGAAATCGAACATGTATACGAAGCTGCTACGTTATTTCTATCTTT
AATCAAGCAAAAAGACTTTGAAGAATTTTTAACTTTACCAGCTTATCAACAATTAAATGAGGGAGGAACTGTATAA

Upstream 100 bases:

>100_bases
TGTTTGTCTAAAAAAGCGGAGTAAATTTTTTTATATACTTTGTTATATAACAAAGTATATAAATTTAAATTATTATACAA
CAGTTAGTGAGGAGGATTTT

Downstream 100 bases:

>100_bases
TGAAAAATGAAAGAGTAGAGAATTTAACTAAAGAGTGGCAAGATGATATTCGTTGGGTGGGGATTGAACGTCCGTATAAA
GCAGAAGATGTAATAAGACT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKDATILPDHKKAKYEDILNTEARKFLLRLHHHFNQRRKELLIERKEKQKRMSEGEKLHFLSLTRQVREADWEIDPVPTD
LKDRRVEITGPVDRKMIINALNSGANTFMADFEDASSPTWENMMEGQRNLKDAIRKDIDFLDNKGKQYRLNDKTAVIIVR
PRGWHLEEHHITVENEPMSASLVDFGLYFFHNAKELLQSGSGPYFYLPKIESYLEARLWNDVFTFAQNEIGIPQGTIKAT
VLIETITAAFEMDEILYELRHHSAGLNCGRWDYIFSFIKKNQHNPAMLLPDRSLVTMETHFMRSYSLLAIQTCHRRNAHA
IGGMAAQIPVKNNEELNRQAFDKVKKDKEREVNDGHDGTWIAHPGMVQLVKDVFDQGMPAANQINRKREDIHVTADDLLK
LPTGPITMEGLRTNIKVGIHYVAAWLAGRGAVPINNLMEDAATAEISRAQVWQWIQNKAMMDNEQQVTFEVVKDIVDEVL
VQFEESLGIEGIEIEHVYEAATLFLSLIKQKDFEEFLTLPAYQQLNEGGTV

Sequences:

>Translated_531_residues
MKDATILPDHKKAKYEDILNTEARKFLLRLHHHFNQRRKELLIERKEKQKRMSEGEKLHFLSLTRQVREADWEIDPVPTD
LKDRRVEITGPVDRKMIINALNSGANTFMADFEDASSPTWENMMEGQRNLKDAIRKDIDFLDNKGKQYRLNDKTAVIIVR
PRGWHLEEHHITVENEPMSASLVDFGLYFFHNAKELLQSGSGPYFYLPKIESYLEARLWNDVFTFAQNEIGIPQGTIKAT
VLIETITAAFEMDEILYELRHHSAGLNCGRWDYIFSFIKKNQHNPAMLLPDRSLVTMETHFMRSYSLLAIQTCHRRNAHA
IGGMAAQIPVKNNEELNRQAFDKVKKDKEREVNDGHDGTWIAHPGMVQLVKDVFDQGMPAANQINRKREDIHVTADDLLK
LPTGPITMEGLRTNIKVGIHYVAAWLAGRGAVPINNLMEDAATAEISRAQVWQWIQNKAMMDNEQQVTFEVVKDIVDEVL
VQFEESLGIEGIEIEHVYEAATLFLSLIKQKDFEEFLTLPAYQQLNEGGTV
>Mature_531_residues
MKDATILPDHKKAKYEDILNTEARKFLLRLHHHFNQRRKELLIERKEKQKRMSEGEKLHFLSLTRQVREADWEIDPVPTD
LKDRRVEITGPVDRKMIINALNSGANTFMADFEDASSPTWENMMEGQRNLKDAIRKDIDFLDNKGKQYRLNDKTAVIIVR
PRGWHLEEHHITVENEPMSASLVDFGLYFFHNAKELLQSGSGPYFYLPKIESYLEARLWNDVFTFAQNEIGIPQGTIKAT
VLIETITAAFEMDEILYELRHHSAGLNCGRWDYIFSFIKKNQHNPAMLLPDRSLVTMETHFMRSYSLLAIQTCHRRNAHA
IGGMAAQIPVKNNEELNRQAFDKVKKDKEREVNDGHDGTWIAHPGMVQLVKDVFDQGMPAANQINRKREDIHVTADDLLK
LPTGPITMEGLRTNIKVGIHYVAAWLAGRGAVPINNLMEDAATAEISRAQVWQWIQNKAMMDNEQQVTFEVVKDIVDEVL
VQFEESLGIEGIEIEHVYEAATLFLSLIKQKDFEEFLTLPAYQQLNEGGTV

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=510, Percent_Identity=49.0196078431373, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17561814, Length=517, Percent_Identity=49.3230174081238, Blast_Score=516, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71982926, Length=407, Percent_Identity=52.3341523341523, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324212, Length=518, Percent_Identity=50.7722007722008, Blast_Score=517, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6322222, Length=532, Percent_Identity=48.8721804511278, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 61117; Mature: 61117

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDATILPDHKKAKYEDILNTEARKFLLRLHHHFNQRRKELLIERKEKQKRMSEGEKLHF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
LSLTRQVREADWEIDPVPTDLKDRRVEITGPVDRKMIINALNSGANTFMADFEDASSPTW
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCH
ENMMEGQRNLKDAIRKDIDFLDNKGKQYRLNDKTAVIIVRPRGWHLEEHHITVENEPMSA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEECCEEEECCCCCCH
SLVDFGLYFFHNAKELLQSGSGPYFYLPKIESYLEARLWNDVFTFAQNEIGIPQGTIKAT
HHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLIETITAAFEMDEILYELRHHSAGLNCGRWDYIFSFIKKNQHNPAMLLPDRSLVTMETH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEHHHH
FMRSYSLLAIQTCHRRNAHAIGGMAAQIPVKNNEELNRQAFDKVKKDKEREVNDGHDGTW
HHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
IAHPGMVQLVKDVFDQGMPAANQINRKREDIHVTADDLLKLPTGPITMEGLRTNIKVGIH
EECCCHHHHHHHHHHCCCCCHHHHHCCHHHEEEEHHHHHHCCCCCEEHHCHHHHHHHHHH
YVAAWLAGRGAVPINNLMEDAATAEISRAQVWQWIQNKAMMDNEQQVTFEVVKDIVDEVL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VQFEESLGIEGIEIEHVYEAATLFLSLIKQKDFEEFLTLPAYQQLNEGGTV
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHCCCCCC
>Mature Secondary Structure
MKDATILPDHKKAKYEDILNTEARKFLLRLHHHFNQRRKELLIERKEKQKRMSEGEKLHF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHH
LSLTRQVREADWEIDPVPTDLKDRRVEITGPVDRKMIINALNSGANTFMADFEDASSPTW
HHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCEEECCCCCCCCCH
ENMMEGQRNLKDAIRKDIDFLDNKGKQYRLNDKTAVIIVRPRGWHLEEHHITVENEPMSA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCEECCEEEECCCCCCH
SLVDFGLYFFHNAKELLQSGSGPYFYLPKIESYLEARLWNDVFTFAQNEIGIPQGTIKAT
HHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLIETITAAFEMDEILYELRHHSAGLNCGRWDYIFSFIKKNQHNPAMLLPDRSLVTMETH
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCEEEHHHH
FMRSYSLLAIQTCHRRNAHAIGGMAAQIPVKNNEELNRQAFDKVKKDKEREVNDGHDGTW
HHHHHHHHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCE
IAHPGMVQLVKDVFDQGMPAANQINRKREDIHVTADDLLKLPTGPITMEGLRTNIKVGIH
EECCCHHHHHHHHHHCCCCCHHHHHCCHHHEEEEHHHHHHCCCCCEEHHCHHHHHHHHHH
YVAAWLAGRGAVPINNLMEDAATAEISRAQVWQWIQNKAMMDNEQQVTFEVVKDIVDEVL
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VQFEESLGIEGIEIEHVYEAATLFLSLIKQKDFEEFLTLPAYQQLNEGGTV
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA