Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 23099749

GI number: 23099749

Start: 2340464

End: 2341105

Strand: Reverse

Name: trmB

Synonym: OB2294

Alternate gene names: 23099749

Gene position: 2341105-2340464 (Counterclockwise)

Preceding gene: 23099751

Following gene: 23099748

Centisome position: 64.48

GC content: 31.31

Gene sequence:

>642_bases
ATGAGGCAAAGAAACAAGCCGTGGGCAGACGAGTATTTACAAGAAAATAATCATATTGTCATTAGTGATCCAAAAAGCAA
AAAAGGGAATTGGAGTCAGCTTTTTAATAATAATAATCCAATTCATGTAGAAATTGGATCTGGAAAAGGGCAATTTATTT
TGGGAATGGCGAAGCAGTATCCAGAGATTAATTTTATTGGAATAGAATTAGCTAAAAGTATTATTGTTTCTGCTGTTCAG
AAAATAGAAGAAGAAAATTTAGCGAATGTTTTTATGTTAAATGAAAATGCTATAGATGTACGTGAAATGTTTGCTGACGA
TGAAATCTCTATGATTTATTTGAATTTTTCAGATCCATGGCCAAAAAATAGACATGAAAAACGACGTTTGACTTACGGCC
GATTTTTAGAACAATATCAGGATATTTTGGGTGCTAATGGAGAAGTTATATTAAAGACAGATAATCGAGGATTATTTGAG
TACTCTGTAGTTAGCTTTTCACAAAATGGAATGAACATAAACGAAATTAATGTTGATTTACATGCTATTCAAGATGAGAC
TAATGTTATGACAGAATATGAAGAGAAATTTTCTACAAAAGGACAACCAATTTATCGCTGTAAAGCAGCATTTGAAAAGT
AG

Upstream 100 bases:

>100_bases
TTGATTTGATACATTTAATGTCATTTTTTATCACCTCTAAACTAGTTTTTTCTGAATAAAGTTTTCTATACAAAATGAAA
TAAAAGTGAGGTAATACAGC

Downstream 100 bases:

>100_bases
TGCTTGACTTTTTCTAGAAATTTGTCATAATATAAAAGAAATCTAGACACCTTTAATTCAGTCCTGTGAGGCTGGCAAGG
TTGTGCGGAAAAGATAAATA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRQRNKPWADEYLQENNHIVISDPKSKKGNWSQLFNNNNPIHVEIGSGKGQFILGMAKQYPEINFIGIELAKSIIVSAVQ
KIEEENLANVFMLNENAIDVREMFADDEISMIYLNFSDPWPKNRHEKRRLTYGRFLEQYQDILGANGEVILKTDNRGLFE
YSVVSFSQNGMNINEINVDLHAIQDETNVMTEYEEKFSTKGQPIYRCKAAFEK

Sequences:

>Translated_213_residues
MRQRNKPWADEYLQENNHIVISDPKSKKGNWSQLFNNNNPIHVEIGSGKGQFILGMAKQYPEINFIGIELAKSIIVSAVQ
KIEEENLANVFMLNENAIDVREMFADDEISMIYLNFSDPWPKNRHEKRRLTYGRFLEQYQDILGANGEVILKTDNRGLFE
YSVVSFSQNGMNINEINVDLHAIQDETNVMTEYEEKFSTKGQPIYRCKAAFEK
>Mature_213_residues
MRQRNKPWADEYLQENNHIVISDPKSKKGNWSQLFNNNNPIHVEIGSGKGQFILGMAKQYPEINFIGIELAKSIIVSAVQ
KIEEENLANVFMLNENAIDVREMFADDEISMIYLNFSDPWPKNRHEKRRLTYGRFLEQYQDILGANGEVILKTDNRGLFE
YSVVSFSQNGMNINEINVDLHAIQDETNVMTEYEEKFSTKGQPIYRCKAAFEK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=179, Percent_Identity=29.0502793296089, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_OCEIH (Q8EP25)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693215.1
- ProteinModelPortal:   Q8EP25
- SMR:   Q8EP25
- GeneID:   1017348
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2294
- NMPDR:   fig|221109.1.peg.2291
- HOGENOM:   HBG304014
- OMA:   LRNKPWA
- BioCyc:   OIHE221109:OB2294-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24703; Mature: 24703

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRNKPWADEYLQENNHIVISDPKSKKGNWSQLFNNNNPIHVEIGSGKGQFILGMAKQY
CCCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEEHHHC
PEINFIGIELAKSIIVSAVQKIEEENLANVFMLNENAIDVREMFADDEISMIYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCCCC
PKNRHEKRRLTYGRFLEQYQDILGANGEVILKTDNRGLFEYSVVSFSQNGMNINEINVDL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCCCEEEEEEEEE
HAIQDETNVMTEYEEKFSTKGQPIYRCKAAFEK
EEECCCHHHHHHHHHHHCCCCCEEEEHHHCCCC
>Mature Secondary Structure
MRQRNKPWADEYLQENNHIVISDPKSKKGNWSQLFNNNNPIHVEIGSGKGQFILGMAKQY
CCCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHCCCCCEEEEECCCCCCEEEEEHHHC
PEINFIGIELAKSIIVSAVQKIEEENLANVFMLNENAIDVREMFADDEISMIYLNFSDPW
CCCEEEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEEEECCCCC
PKNRHEKRRLTYGRFLEQYQDILGANGEVILKTDNRGLFEYSVVSFSQNGMNINEINVDL
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEEECCCCCEEEEEEEEE
HAIQDETNVMTEYEEKFSTKGQPIYRCKAAFEK
EEECCCHHHHHHHHHHHCCCCCEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376