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The map label for this gene is appA [H]

Identifier: 23099717

GI number: 23099717

Start: 2303702

End: 2305501

Strand: Reverse

Name: appA [H]

Synonym: OB2262

Alternate gene names: 23099717

Gene position: 2305501-2303702 (Counterclockwise)

Preceding gene: 23099718

Following gene: 23099716

Centisome position: 63.5

GC content: 38.83

Gene sequence:

>1800_bases
ATGGAAAAGTGGAAACGAATACTGGTGCTTGCTATTCTAATCTTATTTGTATCTGTATTTGCTGTAGCTTGCACAAGTAA
TAATGAAGGTGAAGGGGATACAGAGGAACCTGCTACTGCAGAGGAAGAAGAAGGGGATGGCGATGAAGAGGCTACTGGTG
AACCACAATCAGGTGGAACCATTACTGGTGCAATGGATACTGCTCCATCAGGAGTCTTTAATCCAATATTTTATGAAGAA
GCATATGAAGCAAATATCTTAGACTTCACACACGAAGGTTTATTAAGTCAAGATAAAGAATTAGCATATGGACCTAACTT
AGCTAACGATTGGGAAATCAATGATGATAACACAGAAATCACGCTGAACTTAGAGGAAGGCGTTAAATGGCATGATGGAG
AAGAATTTACTGCAGATGATGTTGTATTTACCTATAAATCGATTGCATCTCCAGGTTATATTGAAGCTGGGGGCGTACGT
GTAGAATATGCAGAGAAATTAGTTGGCTATACAGCATTCAATAGTGGGGAAACAGATGAATTCGAAGGTGTTGTAGCACA
AGATGACTATACAGTAACCTTTAAATTCGAAGAACCAAATGTAACTATTTTGAAGGATGTGGCATTTCCAATTATACCTG
AACATATCTTTGCAGATGTTCCAATTGGAGAGATGCCTTCTCATCCAGCTACGTTAAATGCTGGTGAAGTAATCGGTACT
GGACCATTTAAATTTACTGAAATGCTTGAACGTGAGCAGTACATTTTAGAAAAGCATGCTGATTATTGGAAGGACGAACC
ATACTTAGATCAAATTGTATGGAGAATTGTAGATCAGTCAGTTATGCTTGGACTTTTAGAAAACGGTGAAATTGATTTTA
TCTCTGATCCAAATGGTGTTCCAGCTGCCGATTTTGAAGATGTAAATGCAATGGAACATGTGGAAATTATTGAACAGCCT
GATTTTGGATATCAGTTAATGGGCTTCAAAATTAATCATAGAACGGATGCGGATGTTGAGAGTGGAACAATTGATCCAGC
AAATTGGGAAGTAAATGAAGATATGGGTGAGAAACTTATTCGACAAGCAATGGCATATGCAATTGATCGTCAAGCAATTG
TTGATAACTTACTGTATGGGCATGGATCCGTTATTAATGCTCCAATTGCACAACAGTTCTGGGCGTATGATGAAGCAGCA
ACAACTTCTTATGGATATGATCCAGACAAAGCTGCAGAATTATTAGACGAGGCAGGTTATGTAGATACGAACGATGACGG
CTTCCGTGAAACTCCGGATGGTGAAGAGTGGATCGTAAATCTGAATTATCCAACAGGGAATGCACTTCGTGAACAATCAG
CTCCAATATTAAAGCAATTCTTAGAGGAAGCTGGAATTAAAATTGATCTACGACAACCGAAAGAAATGTCAGCATATGTA
GAAGAGTTAACAAATGATAATACCGACTGGGATTTATATTTGCTTGGTTGGAGTCTAGGAAGTGGAGATCCAGATCCATC
TGGTTTATGGAGATCTGTTGATCCATATAACTTTGGTCGCTGGAACAATGAAGAATCAGATCAGTTAATTGCAGATGCAT
TAACTGCTCCAGATGCATTTGAGCAAAGTTATCGTGAGGAAAAATATGGTGAATGGCAGGGAGTATTCTCTGATGACCTT
CCCGCTCTACTACTTTACGCACAAAATAAAATTTACGGTCATCATGAACGCCTAAATGGTATAGACCCAATGCCATATGG
GTTTATAAACGATCCACATCTATGGTGGGTTTCTGAGTAA

Upstream 100 bases:

>100_bases
TGCTTAACAAAAGGCGCGTACATAATTAGGAGGTGATGTCTCTGTTATCAAATGGTTAAGTTAGTTCGTCTATCAAAGAT
ATTTAAAAGGGGGAAATTTA

Downstream 100 bases:

>100_bases
TAGTATGGGAGCGTGCGTTAGATGGCGTATGCTCCCATCTATTTTTACTAGAAAGATACATTAAGGTGGGAATAACATGA
CCAAATATATTATACGAAGA

Product: oligopeptide ABC transporter oligopeptide-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MEKWKRILVLAILILFVSVFAVACTSNNEGEGDTEEPATAEEEEGDGDEEATGEPQSGGTITGAMDTAPSGVFNPIFYEE
AYEANILDFTHEGLLSQDKELAYGPNLANDWEINDDNTEITLNLEEGVKWHDGEEFTADDVVFTYKSIASPGYIEAGGVR
VEYAEKLVGYTAFNSGETDEFEGVVAQDDYTVTFKFEEPNVTILKDVAFPIIPEHIFADVPIGEMPSHPATLNAGEVIGT
GPFKFTEMLEREQYILEKHADYWKDEPYLDQIVWRIVDQSVMLGLLENGEIDFISDPNGVPAADFEDVNAMEHVEIIEQP
DFGYQLMGFKINHRTDADVESGTIDPANWEVNEDMGEKLIRQAMAYAIDRQAIVDNLLYGHGSVINAPIAQQFWAYDEAA
TTSYGYDPDKAAELLDEAGYVDTNDDGFRETPDGEEWIVNLNYPTGNALREQSAPILKQFLEEAGIKIDLRQPKEMSAYV
EELTNDNTDWDLYLLGWSLGSGDPDPSGLWRSVDPYNFGRWNNEESDQLIADALTAPDAFEQSYREEKYGEWQGVFSDDL
PALLLYAQNKIYGHHERLNGIDPMPYGFINDPHLWWVSE

Sequences:

>Translated_599_residues
MEKWKRILVLAILILFVSVFAVACTSNNEGEGDTEEPATAEEEEGDGDEEATGEPQSGGTITGAMDTAPSGVFNPIFYEE
AYEANILDFTHEGLLSQDKELAYGPNLANDWEINDDNTEITLNLEEGVKWHDGEEFTADDVVFTYKSIASPGYIEAGGVR
VEYAEKLVGYTAFNSGETDEFEGVVAQDDYTVTFKFEEPNVTILKDVAFPIIPEHIFADVPIGEMPSHPATLNAGEVIGT
GPFKFTEMLEREQYILEKHADYWKDEPYLDQIVWRIVDQSVMLGLLENGEIDFISDPNGVPAADFEDVNAMEHVEIIEQP
DFGYQLMGFKINHRTDADVESGTIDPANWEVNEDMGEKLIRQAMAYAIDRQAIVDNLLYGHGSVINAPIAQQFWAYDEAA
TTSYGYDPDKAAELLDEAGYVDTNDDGFRETPDGEEWIVNLNYPTGNALREQSAPILKQFLEEAGIKIDLRQPKEMSAYV
EELTNDNTDWDLYLLGWSLGSGDPDPSGLWRSVDPYNFGRWNNEESDQLIADALTAPDAFEQSYREEKYGEWQGVFSDDL
PALLLYAQNKIYGHHERLNGIDPMPYGFINDPHLWWVSE
>Mature_599_residues
MEKWKRILVLAILILFVSVFAVACTSNNEGEGDTEEPATAEEEEGDGDEEATGEPQSGGTITGAMDTAPSGVFNPIFYEE
AYEANILDFTHEGLLSQDKELAYGPNLANDWEINDDNTEITLNLEEGVKWHDGEEFTADDVVFTYKSIASPGYIEAGGVR
VEYAEKLVGYTAFNSGETDEFEGVVAQDDYTVTFKFEEPNVTILKDVAFPIIPEHIFADVPIGEMPSHPATLNAGEVIGT
GPFKFTEMLEREQYILEKHADYWKDEPYLDQIVWRIVDQSVMLGLLENGEIDFISDPNGVPAADFEDVNAMEHVEIIEQP
DFGYQLMGFKINHRTDADVESGTIDPANWEVNEDMGEKLIRQAMAYAIDRQAIVDNLLYGHGSVINAPIAQQFWAYDEAA
TTSYGYDPDKAAELLDEAGYVDTNDDGFRETPDGEEWIVNLNYPTGNALREQSAPILKQFLEEAGIKIDLRQPKEMSAYV
EELTNDNTDWDLYLLGWSLGSGDPDPSGLWRSVDPYNFGRWNNEESDQLIADALTAPDAFEQSYREEKYGEWQGVFSDDL
PALLLYAQNKIYGHHERLNGIDPMPYGFINDPHLWWVSE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=552, Percent_Identity=24.8188405797101, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787052, Length=510, Percent_Identity=23.5294117647059, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1789887, Length=413, Percent_Identity=21.3075060532688, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=20.7188160676533, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789397, Length=520, Percent_Identity=22.3076923076923, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1787495, Length=484, Percent_Identity=21.6942148760331, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 67216; Mature: 67216

Theoretical pI: Translated: 3.76; Mature: 3.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKWKRILVLAILILFVSVFAVACTSNNEGEGDTEEPATAEEEEGDGDEEATGEPQSGGT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
ITGAMDTAPSGVFNPIFYEEAYEANILDFTHEGLLSQDKELAYGPNLANDWEINDDNTEI
EEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEE
TLNLEEGVKWHDGEEFTADDVVFTYKSIASPGYIEAGGVRVEYAEKLVGYTAFNSGETDE
EEEHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCEECCCEEHHHHHHHHCEEECCCCCCCH
FEGVVAQDDYTVTFKFEEPNVTILKDVAFPIIPEHIFADVPIGEMPSHPATLNAGEVIGT
HCCEEECCCEEEEEEECCCCEEEEECCCCHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEC
GPFKFTEMLEREQYILEKHADYWKDEPYLDQIVWRIVDQSVMLGLLENGEIDFISDPNGV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PAADFEDVNAMEHVEIIEQPDFGYQLMGFKINHRTDADVESGTIDPANWEVNEDMGEKLI
CCCCCHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RQAMAYAIDRQAIVDNLLYGHGSVINAPIAQQFWAYDEAATTSYGYDPDKAAELLDEAGY
HHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCHHHCCCCCCCHHHHHHHHHHCCC
VDTNDDGFRETPDGEEWIVNLNYPTGNALREQSAPILKQFLEEAGIKIDLRQPKEMSAYV
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHH
EELTNDNTDWDLYLLGWSLGSGDPDPSGLWRSVDPYNFGRWNNEESDQLIADALTAPDAF
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHH
EQSYREEKYGEWQGVFSDDLPALLLYAQNKIYGHHERLNGIDPMPYGFINDPHLWWVSE
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MEKWKRILVLAILILFVSVFAVACTSNNEGEGDTEEPATAEEEEGDGDEEATGEPQSGGT
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
ITGAMDTAPSGVFNPIFYEEAYEANILDFTHEGLLSQDKELAYGPNLANDWEINDDNTEI
EEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEE
TLNLEEGVKWHDGEEFTADDVVFTYKSIASPGYIEAGGVRVEYAEKLVGYTAFNSGETDE
EEEHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCEECCCEEHHHHHHHHCEEECCCCCCCH
FEGVVAQDDYTVTFKFEEPNVTILKDVAFPIIPEHIFADVPIGEMPSHPATLNAGEVIGT
HCCEEECCCEEEEEEECCCCEEEEECCCCHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEC
GPFKFTEMLEREQYILEKHADYWKDEPYLDQIVWRIVDQSVMLGLLENGEIDFISDPNGV
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PAADFEDVNAMEHVEIIEQPDFGYQLMGFKINHRTDADVESGTIDPANWEVNEDMGEKLI
CCCCCHHCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
RQAMAYAIDRQAIVDNLLYGHGSVINAPIAQQFWAYDEAATTSYGYDPDKAAELLDEAGY
HHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHCHHHCCCCCCCHHHHHHHHHHCCC
VDTNDDGFRETPDGEEWIVNLNYPTGNALREQSAPILKQFLEEAGIKIDLRQPKEMSAYV
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHH
EELTNDNTDWDLYLLGWSLGSGDPDPSGLWRSVDPYNFGRWNNEESDQLIADALTAPDAF
HHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHH
EQSYREEKYGEWQGVFSDDLPALLLYAQNKIYGHHERLNGIDPMPYGFINDPHLWWVSE
HHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]