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The map label for this gene is dgoD [H]

Identifier: 23099670

GI number: 23099670

Start: 2249209

End: 2250357

Strand: Direct

Name: dgoD [H]

Synonym: OB2215

Alternate gene names: 23099670

Gene position: 2249209-2250357 (Clockwise)

Preceding gene: 23099669

Following gene: 23099671

Centisome position: 61.95

GC content: 38.73

Gene sequence:

>1149_bases
ATGAAAATAACAGATTATAAAATATATCAGGTACCACCTCGTTGGTTGTTTTTAAAAATAGAAACAGATGAAGGTATTGT
AGGTTGGGGAGAACCAGTTATCGAAGGTAGAGCAGCTACTGTGAAAGCGGCAGTTATAGAACTTATGGAAAATTTAATCG
GCAAGGATCCTCAAAGGATTGAAGATCATTGGAATTTAATGTATCGTTCAGGTTTTTATCGAGGCGGTCCCATTTTAATG
AGTGCTATCGCTGGGATTGACCAGGCATTATGGGATATAAAAGGAAAATTCTTCAATGCACCTATCCACCAGTTACTAGG
TGGAGCTTGTAGAGATTCAATAAAAGTATATTCTTGGATTGGAGGAGACAGACCTTCAGATGTTGGAGCAGCTGCTAAAG
AAGTAGTTGATAGAGGATTTCAAGCAGTTAAGATGAACGGGACAGAAGAATTACAATATATTGACTCTCATCAAAAGATT
GATGCGGTAATAGAAAGAATTGCTGCTGTTAGAGAATCGGTTGGTCCCTATGTTGGAATTGGAATTGATTTTCACGGTCG
TGTCCATAAACCGATGGCTAAAAATTTAGCAAAAGAATTAGAACAATTTAGACCGATGTTCATCGAGGAACCTGTACTCC
CAGAAAATAATGAAGCGTTGAGAGATATCGCAAACCATGTGACCACTCCAATTGCAACTGGTGAAAGAATGTTCTCTCGC
TGGGATTTTAAACAGCTATTAGCAGATGGATATGTAGACATAATACAACCAGACCTTTCACATGCAGGCGGAATAACAGA
AGCGAAAAAAATCTTTTCCATGGCAGAAGCGTATGACGTTGCAGTGGCTCCCCATTGCCCATTAGGGCCAATTGCTCTAG
CTTCATGTCTTCAGGTTGATGCATCTTCTCATAATGCATTTATTCAAGAGCAAAGTTTAGGAATCCACTATAACGTAGGA
AATGATTTATTAGACTATATTGTTGATAAAGACGTGTTCACCTATGAAAATGGTTATGTCAAAATTCCAGACGGACCAGG
ACTAGGTATCGAAGTGAATGAAGAATATGTTAAAGAAAAAGCAAATGATGCAGAGAACTGGCGTAATCCAATTTGGAGAC
ATAAAGACGGGTCAATTGCAGAATGGTAG

Upstream 100 bases:

>100_bases
TAATACCGTAAATGTTACAGATGAATTCTTAGAACAAGTAACAAACAATGCAAAGCAATTCACATCAAAAATGAAAACGT
TATAAATTGGAGTTGATCAT

Downstream 100 bases:

>100_bases
TTTTCAAAAATTAAAGTTTTTATTACAAAGGAGGATTTCTTATGTCCAACACTACTATGTTGATTATGGTTACGATACTT
GGTATAGCGCTATTACTTTT

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITDYKIYQVPPRWLFLKIETDEGIVGWGEPVIEGRAATVKAAVIELMENLIGKDPQRIEDHWNLMYRSGFYRGGPILM
SAIAGIDQALWDIKGKFFNAPIHQLLGGACRDSIKVYSWIGGDRPSDVGAAAKEVVDRGFQAVKMNGTEELQYIDSHQKI
DAVIERIAAVRESVGPYVGIGIDFHGRVHKPMAKNLAKELEQFRPMFIEEPVLPENNEALRDIANHVTTPIATGERMFSR
WDFKQLLADGYVDIIQPDLSHAGGITEAKKIFSMAEAYDVAVAPHCPLGPIALASCLQVDASSHNAFIQEQSLGIHYNVG
NDLLDYIVDKDVFTYENGYVKIPDGPGLGIEVNEEYVKEKANDAENWRNPIWRHKDGSIAEW

Sequences:

>Translated_382_residues
MKITDYKIYQVPPRWLFLKIETDEGIVGWGEPVIEGRAATVKAAVIELMENLIGKDPQRIEDHWNLMYRSGFYRGGPILM
SAIAGIDQALWDIKGKFFNAPIHQLLGGACRDSIKVYSWIGGDRPSDVGAAAKEVVDRGFQAVKMNGTEELQYIDSHQKI
DAVIERIAAVRESVGPYVGIGIDFHGRVHKPMAKNLAKELEQFRPMFIEEPVLPENNEALRDIANHVTTPIATGERMFSR
WDFKQLLADGYVDIIQPDLSHAGGITEAKKIFSMAEAYDVAVAPHCPLGPIALASCLQVDASSHNAFIQEQSLGIHYNVG
NDLLDYIVDKDVFTYENGYVKIPDGPGLGIEVNEEYVKEKANDAENWRNPIWRHKDGSIAEW
>Mature_382_residues
MKITDYKIYQVPPRWLFLKIETDEGIVGWGEPVIEGRAATVKAAVIELMENLIGKDPQRIEDHWNLMYRSGFYRGGPILM
SAIAGIDQALWDIKGKFFNAPIHQLLGGACRDSIKVYSWIGGDRPSDVGAAAKEVVDRGFQAVKMNGTEELQYIDSHQKI
DAVIERIAAVRESVGPYVGIGIDFHGRVHKPMAKNLAKELEQFRPMFIEEPVLPENNEALRDIANHVTTPIATGERMFSR
WDFKQLLADGYVDIIQPDLSHAGGITEAKKIFSMAEAYDVAVAPHCPLGPIALASCLQVDASSHNAFIQEQSLGIHYNVG
NDLLDYIVDKDVFTYENGYVKIPDGPGLGIEVNEEYVKEKANDAENWRNPIWRHKDGSIAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=417, Percent_Identity=24.220623501199, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI186972148, Length=222, Percent_Identity=26.5765765765766, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=60.9947643979058, Blast_Score=492, Evalue=1e-140,
Organism=Escherichia coli, GI1787864, Length=416, Percent_Identity=33.1730769230769, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI226510960, Length=274, Percent_Identity=28.4671532846715, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 42704; Mature: 42704

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDYKIYQVPPRWLFLKIETDEGIVGWGEPVIEGRAATVKAAVIELMENLIGKDPQRI
CCCCCEEEEECCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
EDHWNLMYRSGFYRGGPILMSAIAGIDQALWDIKGKFFNAPIHQLLGGACRDSIKVYSWI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCHHHHCCCEEEEEC
GGDRPSDVGAAAKEVVDRGFQAVKMNGTEELQYIDSHQKIDAVIERIAAVRESVGPYVGI
CCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GIDFHGRVHKPMAKNLAKELEQFRPMFIEEPVLPENNEALRDIANHVTTPIATGERMFSR
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
WDFKQLLADGYVDIIQPDLSHAGGITEAKKIFSMAEAYDVAVAPHCPLGPIALASCLQVD
CCHHHHHHCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHC
ASSHNAFIQEQSLGIHYNVGNDLLDYIVDKDVFTYENGYVKIPDGPGLGIEVNEEYVKEK
CCCCCCEEEECCCEEEEECCHHHHHHHHCCCCEEECCCEEECCCCCCCCEEECHHHHHHH
ANDAENWRNPIWRHKDGSIAEW
CCCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITDYKIYQVPPRWLFLKIETDEGIVGWGEPVIEGRAATVKAAVIELMENLIGKDPQRI
CCCCCEEEEECCCCEEEEEEECCCCCEECCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHH
EDHWNLMYRSGFYRGGPILMSAIAGIDQALWDIKGKFFNAPIHQLLGGACRDSIKVYSWI
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHCHHHHCCCEEEEEC
GGDRPSDVGAAAKEVVDRGFQAVKMNGTEELQYIDSHQKIDAVIERIAAVRESVGPYVGI
CCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
GIDFHGRVHKPMAKNLAKELEQFRPMFIEEPVLPENNEALRDIANHVTTPIATGERMFSR
EEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
WDFKQLLADGYVDIIQPDLSHAGGITEAKKIFSMAEAYDVAVAPHCPLGPIALASCLQVD
CCHHHHHHCCCHHHCCCCHHHCCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHC
ASSHNAFIQEQSLGIHYNVGNDLLDYIVDKDVFTYENGYVKIPDGPGLGIEVNEEYVKEK
CCCCCCEEEECCCEEEEECCHHHHHHHHCCCCEEECCCEEECCCCCCCCEEECHHHHHHH
ANDAENWRNPIWRHKDGSIAEW
CCCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA