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The map label for this gene is gntU [H]

Identifier: 23099671

GI number: 23099671

Start: 2250399

End: 2251760

Strand: Direct

Name: gntU [H]

Synonym: OB2216

Alternate gene names: 23099671

Gene position: 2250399-2251760 (Clockwise)

Preceding gene: 23099670

Following gene: 23099674

Centisome position: 61.99

GC content: 35.1

Gene sequence:

>1362_bases
ATGTCCAACACTACTATGTTGATTATGGTTACGATACTTGGTATAGCGCTATTACTTTTCTTGGTTATGCGATCAAAGCT
GCAAGCTTTTGTTGCATTACTTATTTCCAGTATCTTTATCGGTATTTTATCCGGAATGGATTTAAATGATCTTATAGCTA
CCATGGAAGAAGGTATGGGAGGAACCTTAGGTTTTATTGCAGTCGTAGTTGGCCTTGGTGCAATGTTCGGTGAAATGCTC
CGAGTTTCTGGGGGCGCGGAACGTTTAGCATTAACGTTAGTTAATAAATTTGGCGATGGGAAAGTTCAGTGGGCACTTGG
TTTAACAGGGTTTATTGTAGCAATACCGGTTTTTCTAGATGTTGCCTTGGTAATCTTAATCCCAATTGTATATAGTTTAG
CTCAAAAAACGAAAAAATCATTATTATATTTCGGTATCCCTTTACTAGCAGGATTAGCTGTTACCCATAGTTTCATACCA
CCTACTCCAGGACCAATATCGGTGGCTTCTATTATAAATGCCGATCTAGGTTGGGTAATTTTATTTGGTGCTTTAGCAGG
TTTACCTGCGATGATTATAGCAGGACCGATATTTGGTAATTATATTTCTAAAAAAATTCATTTAGAAGTTCCTAAATATA
TTGTAGATAAAGAATATCTTGAAAACGAAGTAGAAAAAACGAATCGTGAACTACCAAGCTTTAAAACTGTATGTCTATTA
ATATTAATTCCACTCTTTTTAATACTTATTAACACTGTATTTGGATTAATATTAGAAGAAGGAACGTTTATACATTCATT
CCTTACATTTATCGGACATCCATTTATTGCTTTAACAATATCTACCCTATTAACATTCTATATATTGGGTACAAAAAGAG
GATATTCAAAAGAAGAAGTACAGAATATTGCAACGAAATCATTAGAACCTGCAGGTATTATTATCCTTATTACTGGTGCA
GGCGGAGTTTTCAAACAAACGTTAATTGATAGTGGTGTAGGTGATGTACTAGGTAATATGTTAGCGACATCAAACCTTCC
TTTAGTACTGGCAGCATTCATCATTTCGACTTTTGTGCGAATAGCACAAGGTTCAGCTACAGTTGCTATGATTACTGCTG
CAGGCTTAATGTCGCCAATTATTGGTATGATGGAAGTTTCTGAACCTATGTTAGGTTTAATTGTTATATCCATAGCGAGT
GGTGCTACAGTATTCTCTCATGTAAATGATTCAGGATTCTGGTTAGTTAATAGATTTTTCGGAATGACAGAAAAGCAAAC
CTTACAAACATGGACAGTAATGGAAACTATCATTGGTTTTGTTGGATTTGCAGTCGTATTTGCAATTAGCTTCTTTGTCT
AA

Upstream 100 bases:

>100_bases
CAGAGAACTGGCGTAATCCAATTTGGAGACATAAAGACGGGTCAATTGCAGAATGGTAGTTTTCAAAAATTAAAGTTTTT
ATTACAAAGGAGGATTTCTT

Downstream 100 bases:

>100_bases
CATATAAAAATATAGTAGTAGATAGGGACATGAATTCGGATTGACAACCCGCTTCAGAAATATACTCTGCTTTCCCTGGG
TGGCTGGTAAGCCTCCGTTT

Product: low-affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSNTTMLIMVTILGIALLLFLVMRSKLQAFVALLISSIFIGILSGMDLNDLIATMEEGMGGTLGFIAVVVGLGAMFGEML
RVSGGAERLALTLVNKFGDGKVQWALGLTGFIVAIPVFLDVALVILIPIVYSLAQKTKKSLLYFGIPLLAGLAVTHSFIP
PTPGPISVASIINADLGWVILFGALAGLPAMIIAGPIFGNYISKKIHLEVPKYIVDKEYLENEVEKTNRELPSFKTVCLL
ILIPLFLILINTVFGLILEEGTFIHSFLTFIGHPFIALTISTLLTFYILGTKRGYSKEEVQNIATKSLEPAGIIILITGA
GGVFKQTLIDSGVGDVLGNMLATSNLPLVLAAFIISTFVRIAQGSATVAMITAAGLMSPIIGMMEVSEPMLGLIVISIAS
GATVFSHVNDSGFWLVNRFFGMTEKQTLQTWTVMETIIGFVGFAVVFAISFFV

Sequences:

>Translated_453_residues
MSNTTMLIMVTILGIALLLFLVMRSKLQAFVALLISSIFIGILSGMDLNDLIATMEEGMGGTLGFIAVVVGLGAMFGEML
RVSGGAERLALTLVNKFGDGKVQWALGLTGFIVAIPVFLDVALVILIPIVYSLAQKTKKSLLYFGIPLLAGLAVTHSFIP
PTPGPISVASIINADLGWVILFGALAGLPAMIIAGPIFGNYISKKIHLEVPKYIVDKEYLENEVEKTNRELPSFKTVCLL
ILIPLFLILINTVFGLILEEGTFIHSFLTFIGHPFIALTISTLLTFYILGTKRGYSKEEVQNIATKSLEPAGIIILITGA
GGVFKQTLIDSGVGDVLGNMLATSNLPLVLAAFIISTFVRIAQGSATVAMITAAGLMSPIIGMMEVSEPMLGLIVISIAS
GATVFSHVNDSGFWLVNRFFGMTEKQTLQTWTVMETIIGFVGFAVVFAISFFV
>Mature_452_residues
SNTTMLIMVTILGIALLLFLVMRSKLQAFVALLISSIFIGILSGMDLNDLIATMEEGMGGTLGFIAVVVGLGAMFGEMLR
VSGGAERLALTLVNKFGDGKVQWALGLTGFIVAIPVFLDVALVILIPIVYSLAQKTKKSLLYFGIPLLAGLAVTHSFIPP
TPGPISVASIINADLGWVILFGALAGLPAMIIAGPIFGNYISKKIHLEVPKYIVDKEYLENEVEKTNRELPSFKTVCLLI
LIPLFLILINTVFGLILEEGTFIHSFLTFIGHPFIALTISTLLTFYILGTKRGYSKEEVQNIATKSLEPAGIIILITGAG
GVFKQTLIDSGVGDVLGNMLATSNLPLVLAAFIISTFVRIAQGSATVAMITAAGLMSPIIGMMEVSEPMLGLIVISIASG
ATVFSHVNDSGFWLVNRFFGMTEKQTLQTWTVMETIIGFVGFAVVFAISFFV

Specific function: Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994939, Length=449, Percent_Identity=45.43429844098, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI48994935, Length=441, Percent_Identity=38.5487528344671, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790716, Length=437, Percent_Identity=36.8421052631579, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1789097, Length=453, Percent_Identity=37.3068432671082, Blast_Score=288, Evalue=4e-79,
Organism=Escherichia coli, GI2367370, Length=442, Percent_Identity=36.8778280542986, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI1790776, Length=448, Percent_Identity=34.1517857142857, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1788707, Length=458, Percent_Identity=33.4061135371179, Blast_Score=247, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 48479; Mature: 48348

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTTMLIMVTILGIALLLFLVMRSKLQAFVALLISSIFIGILSGMDLNDLIATMEEGMG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GTLGFIAVVVGLGAMFGEMLRVSGGAERLALTLVNKFGDGKVQWALGLTGFIVAIPVFLD
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
VALVILIPIVYSLAQKTKKSLLYFGIPLLAGLAVTHSFIPPTPGPISVASIINADLGWVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHH
LFGALAGLPAMIIAGPIFGNYISKKIHLEVPKYIVDKEYLENEVEKTNRELPSFKTVCLL
HHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILIPLFLILINTVFGLILEEGTFIHSFLTFIGHPFIALTISTLLTFYILGTKRGYSKEEV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
QNIATKSLEPAGIIILITGAGGVFKQTLIDSGVGDVLGNMLATSNLPLVLAAFIISTFVR
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAQGSATVAMITAAGLMSPIIGMMEVSEPMLGLIVISIASGATVFSHVNDSGFWLVNRFF
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHH
GMTEKQTLQTWTVMETIIGFVGFAVVFAISFFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNTTMLIMVTILGIALLLFLVMRSKLQAFVALLISSIFIGILSGMDLNDLIATMEEGMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
GTLGFIAVVVGLGAMFGEMLRVSGGAERLALTLVNKFGDGKVQWALGLTGFIVAIPVFLD
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHH
VALVILIPIVYSLAQKTKKSLLYFGIPLLAGLAVTHSFIPPTPGPISVASIINADLGWVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHH
LFGALAGLPAMIIAGPIFGNYISKKIHLEVPKYIVDKEYLENEVEKTNRELPSFKTVCLL
HHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILIPLFLILINTVFGLILEEGTFIHSFLTFIGHPFIALTISTLLTFYILGTKRGYSKEEV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
QNIATKSLEPAGIIILITGAGGVFKQTLIDSGVGDVLGNMLATSNLPLVLAAFIISTFVR
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IAQGSATVAMITAAGLMSPIIGMMEVSEPMLGLIVISIASGATVFSHVNDSGFWLVNRFF
HHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHH
GMTEKQTLQTWTVMETIIGFVGFAVVFAISFFV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]