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The map label for this gene is iclR [H]

Identifier: 23099667

GI number: 23099667

Start: 2246811

End: 2247557

Strand: Direct

Name: iclR [H]

Synonym: OB2212

Alternate gene names: 23099667

Gene position: 2246811-2247557 (Clockwise)

Preceding gene: 23099660

Following gene: 23099668

Centisome position: 61.89

GC content: 35.07

Gene sequence:

>747_bases
GTGTCTGTTAAATCTGCTAAGCGTGCTTTGGACATTCTTGACTTATTAGCAAAACATACTAACGGTCTAACCATTAAAAA
CATCGGCGACAAGTTAGAATTACCACAAAGCAGTACGTTTAATCTTTTAAAAACATTACATAGTGAAGGATACGTACGAC
AGGATAGCATGAAACTATATTACTTAGGTGAAAAGTTAATACCTCTAGGAACAAGTGCTTTAGAATCACTAGATATTCAC
ACGGTTGCAAAACCTTATTTAGCCGATCTAATGAAAGCGGTTAAGGAAACTGTATTTATGGCGATGTTATCTAAAGATGA
GTTAGTGTATATTGCAAAAATGGATGCTGACCGGTCGATACGAACAACGGCACAACCCGGATATAAAAAGCCAATATATT
GTACCGGACTTGGCAAAACATTCTTAGCTTTTATGCCTGAAACAGAGAAAAACAGCTTATTAAATCACATAACATTAGAG
AAAATCACTGCAAATACAATTACCGATAGATCTCAATTAGAAGAACAACTGCAATTATTTCAGAAACAAGGATACAGTAT
TGATGATGAAGAAAATGAAGAAGGTCTCTACTGTTTAGCTGCTCCCATCTTTGATAGTCAAAGACAGCTTTTAGCAGCCG
TAAGTGTGGCAGGTCCAAAAGATAGAATGCTAAAACAAAAAGAAACAGTTATTACAAACTTAAGAGATACAGCCAAACTT
ATTTCAAATGGTATGGGATATGTTTAA

Upstream 100 bases:

>100_bases
TTAAAAAAATAGTCTTTACGAGATAAATAAAGTGTGGTATATTCAACAAAATCACATACATGATTATTATTCACATATGT
GAATTTAAGGGGGTGGAATA

Downstream 100 bases:

>100_bases
GGAGAGGAGTATGAAAAGAATGGATGTTATTGGAATCGGAGAAACAATGGTATCACTAACCCCAGATAAATACGGTCTTA
TTAGGCAAACGTCCTCCTTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSVKSAKRALDILDLLAKHTNGLTIKNIGDKLELPQSSTFNLLKTLHSEGYVRQDSMKLYYLGEKLIPLGTSALESLDIH
TVAKPYLADLMKAVKETVFMAMLSKDELVYIAKMDADRSIRTTAQPGYKKPIYCTGLGKTFLAFMPETEKNSLLNHITLE
KITANTITDRSQLEEQLQLFQKQGYSIDDEENEEGLYCLAAPIFDSQRQLLAAVSVAGPKDRMLKQKETVITNLRDTAKL
ISNGMGYV

Sequences:

>Translated_248_residues
MSVKSAKRALDILDLLAKHTNGLTIKNIGDKLELPQSSTFNLLKTLHSEGYVRQDSMKLYYLGEKLIPLGTSALESLDIH
TVAKPYLADLMKAVKETVFMAMLSKDELVYIAKMDADRSIRTTAQPGYKKPIYCTGLGKTFLAFMPETEKNSLLNHITLE
KITANTITDRSQLEEQLQLFQKQGYSIDDEENEEGLYCLAAPIFDSQRQLLAAVSVAGPKDRMLKQKETVITNLRDTAKL
ISNGMGYV
>Mature_247_residues
SVKSAKRALDILDLLAKHTNGLTIKNIGDKLELPQSSTFNLLKTLHSEGYVRQDSMKLYYLGEKLIPLGTSALESLDIHT
VAKPYLADLMKAVKETVFMAMLSKDELVYIAKMDADRSIRTTAQPGYKKPIYCTGLGKTFLAFMPETEKNSLLNHITLEK
ITANTITDRSQLEEQLQLFQKQGYSIDDEENEEGLYCLAAPIFDSQRQLLAAVSVAGPKDRMLKQKETVITNLRDTAKLI
SNGMGYV

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=230, Percent_Identity=34.7826086956522, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1786715, Length=249, Percent_Identity=32.1285140562249, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=31.2, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=31.4516129032258, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788581, Length=218, Percent_Identity=30.2752293577982, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789999, Length=250, Percent_Identity=32.8, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790752, Length=220, Percent_Identity=30, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 27727; Mature: 27596

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKSAKRALDILDLLAKHTNGLTIKNIGDKLELPQSSTFNLLKTLHSEGYVRQDSMKLY
CCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEECCCEEEE
YLGEKLIPLGTSALESLDIHTVAKPYLADLMKAVKETVFMAMLSKDELVYIAKMDADRSI
EECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
RTTAQPGYKKPIYCTGLGKTFLAFMPETEKNSLLNHITLEKITANTITDRSQLEEQLQLF
ECCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QKQGYSIDDEENEEGLYCLAAPIFDSQRQLLAAVSVAGPKDRMLKQKETVITNLRDTAKL
HHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ISNGMGYV
HHCCCCCC
>Mature Secondary Structure 
SVKSAKRALDILDLLAKHTNGLTIKNIGDKLELPQSSTFNLLKTLHSEGYVRQDSMKLY
CCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCEECCCEEEE
YLGEKLIPLGTSALESLDIHTVAKPYLADLMKAVKETVFMAMLSKDELVYIAKMDADRSI
EECCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCE
RTTAQPGYKKPIYCTGLGKTFLAFMPETEKNSLLNHITLEKITANTITDRSQLEEQLQLF
ECCCCCCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QKQGYSIDDEENEEGLYCLAAPIFDSQRQLLAAVSVAGPKDRMLKQKETVITNLRDTAKL
HHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ISNGMGYV
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]