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The map label for this gene is sigK [H]

Identifier: 23099454

GI number: 23099454

Start: 2024046

End: 2024750

Strand: Direct

Name: sigK [H]

Synonym: OB1999

Alternate gene names: 23099454

Gene position: 2024046-2024750 (Clockwise)

Preceding gene: 23099453

Following gene: 23099472

Centisome position: 55.75

GC content: 34.47

Gene sequence:

>705_bases
TTGTCCGGTATATTATCCGTACTTGGCCTTATTATTAAAGAGGCATTGTTTTTTGTATCTTATGTAAAGAATCATTCTTT
TCCGCAGCCCCTCCCCCCAGATAAAGAATCTAAATATCTCCAACTTATGCAAGAAGGTGACGAGGTCGCAAGAAACAAAC
TCATTGAACATAATCTTCGTCTTGTTGCACACATTGTAAAAAAATTCGAAAACACTGGTGAGGATATGGAGGATTTAATA
TCCATCGGTACAATTGGTCTAATTAAAGGCGTAGAAAGCTTTTCTACTGATAAGGGTACTAAATTAGCCACATACGCTGC
TCGATGCATTGAGAATGAGATTTTAATGCATTTGCGTGCCTTAAAGAAGGTTAAGAAAGATGTCTCCCTTCATGACCCAA
TTGGCCAAGACAAAGAAGGAAATGAAATCAGTTTGATTGATATTTTAGAAGCAGAGAATGAAAATGTAATTGAGTATATT
CAGTTAAATATGGAGATATCAAAAATGCAGAAGTATTTTTCTGTATTGGATAAACGAGAACGGGAAGTCATTGTTTATCG
TTATGGGTTAAATGATCGAGAAGAAATGACTCAACGTGAGATTGCTAAGAAATTAAACATATCTAGAAGTTATGTATCAA
GAATTGAAAAAAGGGCATTAATGAAAGTATTTCATGAGTATTATCGGAAGGAAAGAGACAATTAA

Upstream 100 bases:

>100_bases
ATGGACAGACACGATTTCACTTCTTCACTCATAAAGTGTTACATAGGCTAATACCTAGCTACTTTGACACATATGCTACA
GTTTTAGGAGGTGATCGCGT

Downstream 100 bases:

>100_bases
AAGAGAACGCTGTCGGATCATGCATTTTTATGCAACGTAAGTGCGACTAGGTAATGATTTGCTCGCTATCGAGGTTTTCA
ATTTTTTAAGTATCACTGCA

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSGILSVLGLIIKEALFFVSYVKNHSFPQPLPPDKESKYLQLMQEGDEVARNKLIEHNLRLVAHIVKKFENTGEDMEDLI
SIGTIGLIKGVESFSTDKGTKLATYAARCIENEILMHLRALKKVKKDVSLHDPIGQDKEGNEISLIDILEAENENVIEYI
QLNMEISKMQKYFSVLDKREREVIVYRYGLNDREEMTQREIAKKLNISRSYVSRIEKRALMKVFHEYYRKERDN

Sequences:

>Translated_234_residues
MSGILSVLGLIIKEALFFVSYVKNHSFPQPLPPDKESKYLQLMQEGDEVARNKLIEHNLRLVAHIVKKFENTGEDMEDLI
SIGTIGLIKGVESFSTDKGTKLATYAARCIENEILMHLRALKKVKKDVSLHDPIGQDKEGNEISLIDILEAENENVIEYI
QLNMEISKMQKYFSVLDKREREVIVYRYGLNDREEMTQREIAKKLNISRSYVSRIEKRALMKVFHEYYRKERDN
>Mature_233_residues
SGILSVLGLIIKEALFFVSYVKNHSFPQPLPPDKESKYLQLMQEGDEVARNKLIEHNLRLVAHIVKKFENTGEDMEDLIS
IGTIGLIKGVESFSTDKGTKLATYAARCIENEILMHLRALKKVKKDVSLHDPIGQDKEGNEISLIDILEAENENVIEYIQ
LNMEISKMQKYFSVLDKREREVIVYRYGLNDREEMTQREIAKKLNISRSYVSRIEKRALMKVFHEYYRKERDN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=256, Percent_Identity=26.171875, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27224; Mature: 27093

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGILSVLGLIIKEALFFVSYVKNHSFPQPLPPDKESKYLQLMQEGDEVARNKLIEHNLR
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVAHIVKKFENTGEDMEDLISIGTIGLIKGVESFSTDKGTKLATYAARCIENEILMHLRA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LKKVKKDVSLHDPIGQDKEGNEISLIDILEAENENVIEYIQLNMEISKMQKYFSVLDKRE
HHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
REVIVYRYGLNDREEMTQREIAKKLNISRSYVSRIEKRALMKVFHEYYRKERDN
CEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGILSVLGLIIKEALFFVSYVKNHSFPQPLPPDKESKYLQLMQEGDEVARNKLIEHNLR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVAHIVKKFENTGEDMEDLISIGTIGLIKGVESFSTDKGTKLATYAARCIENEILMHLRA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LKKVKKDVSLHDPIGQDKEGNEISLIDILEAENENVIEYIQLNMEISKMQKYFSVLDKRE
HHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
REVIVYRYGLNDREEMTQREIAKKLNISRSYVSRIEKRALMKVFHEYYRKERDN
CEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]