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The map label for this gene is holA [H]

Identifier: 23099432

GI number: 23099432

Start: 2005435

End: 2006448

Strand: Reverse

Name: holA [H]

Synonym: OB1977

Alternate gene names: 23099432

Gene position: 2006448-2005435 (Counterclockwise)

Preceding gene: 23099434

Following gene: 23099430

Centisome position: 55.27

GC content: 32.54

Gene sequence:

>1014_bases
ATGTCAATAACGAATATAGTAAATGATATTAAGAAGTCCGTTCAACCTGTTTACCTTGCATTTGGGACGGAGTCATATTT
TTTAGAAGAACTGAAACAGGCTCTGATTAAACAAGTATTAAATGGTGATACAAGTAATTTATCGTTATACGATTTAAATG
AAATGCCGATTCAAGAAGTTCTTACAGATGCAGAGACATTCCCTTTTTTTGAAGAAAAAAAACTGATTATAGCTACCAAT
GCATCATTTTTAAAAGCAAGACCGGACAAGCTTTCTTTTGAACATAATACTTCTTATTTAGAAGAGTATGTTCAAAATCC
TCCAGATTATACAGTTTTATTAATTATTGCTCCTTACGAAAAATTAGATGAACGCAAAAAGATAACAAAACAACTTAAAA
ATAATTCTCAACTTGTGGACTGCCAGGAAATTAGAGAACAGGATGCAAGAAAATGGCTAGAATCAATCATATCAAAAAAC
AATATAACAATGGATGCAAAAGCTAAAGATATGCTAGAAGCAGAAGTTACGACTAATATTCAGCTATTACAGAGTGAAAT
AGAGAAAATGGCGTTATACGTTGGAGAGGGTGGACATGTCAGTGAGGAAGATGCTCAACGATTGATTTCTCACACTCCAA
CAAGCACCTCATTAGCAATGGTAGATGCAGTTATGCATGGAGAAATTCAGCGAGCAATGAGAATTTATAAAGATTTAGAA
AAGATGAATGAAGATCCTATTGCAATGATCGGATTATTATCTTATCAGTTCAGAATGATCCTTCGGGTAAAATTAATGAA
GCAAAAGGGATATACACAGTTCCAAATGCAAAAACAAATTGGTGCTCATCCATATGTTATTAAAATTGCAATGTCAAGAG
AAAAAAATATTTCACAGGATAAATTAGAACAGATAATCCAGCGACTAGCTGAAACGGATAGTGTAATGAAACAAGGAAAG
ATGGAAAAAAGTTTAGCGTTTGAACTATTACTTATGGAATTAATAGTTGCATAA

Upstream 100 bases:

>100_bases
GCAAATATATTTTTCTCCTCCTAATAAATAAAATATCACTTGCTATTCATTTCATTCCATTTTAACATATTGATATAATA
TACATAGGTGTGTGAATGAA

Downstream 100 bases:

>100_bases
ATAAAGCCGAATGAACTCCAAGGTTCATTCGGCTTTTTTTTCTAAAAGGAGATCTTTATCCTAATAGTATTATAATGCGT
TTATTTTGTTAGCTAAACGA

Product: DNA polymerase III subunit delta

Products: diphosphate; DNAn+1

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSITNIVNDIKKSVQPVYLAFGTESYFLEELKQALIKQVLNGDTSNLSLYDLNEMPIQEVLTDAETFPFFEEKKLIIATN
ASFLKARPDKLSFEHNTSYLEEYVQNPPDYTVLLIIAPYEKLDERKKITKQLKNNSQLVDCQEIREQDARKWLESIISKN
NITMDAKAKDMLEAEVTTNIQLLQSEIEKMALYVGEGGHVSEEDAQRLISHTPTSTSLAMVDAVMHGEIQRAMRIYKDLE
KMNEDPIAMIGLLSYQFRMILRVKLMKQKGYTQFQMQKQIGAHPYVIKIAMSREKNISQDKLEQIIQRLAETDSVMKQGK
MEKSLAFELLLMELIVA

Sequences:

>Translated_337_residues
MSITNIVNDIKKSVQPVYLAFGTESYFLEELKQALIKQVLNGDTSNLSLYDLNEMPIQEVLTDAETFPFFEEKKLIIATN
ASFLKARPDKLSFEHNTSYLEEYVQNPPDYTVLLIIAPYEKLDERKKITKQLKNNSQLVDCQEIREQDARKWLESIISKN
NITMDAKAKDMLEAEVTTNIQLLQSEIEKMALYVGEGGHVSEEDAQRLISHTPTSTSLAMVDAVMHGEIQRAMRIYKDLE
KMNEDPIAMIGLLSYQFRMILRVKLMKQKGYTQFQMQKQIGAHPYVIKIAMSREKNISQDKLEQIIQRLAETDSVMKQGK
MEKSLAFELLLMELIVA
>Mature_336_residues
SITNIVNDIKKSVQPVYLAFGTESYFLEELKQALIKQVLNGDTSNLSLYDLNEMPIQEVLTDAETFPFFEEKKLIIATNA
SFLKARPDKLSFEHNTSYLEEYVQNPPDYTVLLIIAPYEKLDERKKITKQLKNNSQLVDCQEIREQDARKWLESIISKNN
ITMDAKAKDMLEAEVTTNIQLLQSEIEKMALYVGEGGHVSEEDAQRLISHTPTSTSLAMVDAVMHGEIQRAMRIYKDLEK
MNEDPIAMIGLLSYQFRMILRVKLMKQKGYTQFQMQKQIGAHPYVIKIAMSREKNISQDKLEQIIQRLAETDSVMKQGKM
EKSLAFELLLMELIVA

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: 2.7.7.7

Molecular weight: Translated: 38771; Mature: 38639

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITNIVNDIKKSVQPVYLAFGTESYFLEELKQALIKQVLNGDTSNLSLYDLNEMPIQEV
CCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
LTDAETFPFFEEKKLIIATNASFLKARPDKLSFEHNTSYLEEYVQNPPDYTVLLIIAPYE
HCCHHHCCCCCCCEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHH
KLDERKKITKQLKNNSQLVDCQEIREQDARKWLESIISKNNITMDAKAKDMLEAEVTTNI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
QLLQSEIEKMALYVGEGGHVSEEDAQRLISHTPTSTSLAMVDAVMHGEIQRAMRIYKDLE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KMNEDPIAMIGLLSYQFRMILRVKLMKQKGYTQFQMQKQIGAHPYVIKIAMSREKNISQD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHH
KLEQIIQRLAETDSVMKQGKMEKSLAFELLLMELIVA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SITNIVNDIKKSVQPVYLAFGTESYFLEELKQALIKQVLNGDTSNLSLYDLNEMPIQEV
CHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHH
LTDAETFPFFEEKKLIIATNASFLKARPDKLSFEHNTSYLEEYVQNPPDYTVLLIIAPYE
HCCHHHCCCCCCCEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEECCHH
KLDERKKITKQLKNNSQLVDCQEIREQDARKWLESIISKNNITMDAKAKDMLEAEVTTNI
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHH
QLLQSEIEKMALYVGEGGHVSEEDAQRLISHTPTSTSLAMVDAVMHGEIQRAMRIYKDLE
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KMNEDPIAMIGLLSYQFRMILRVKLMKQKGYTQFQMQKQIGAHPYVIKIAMSREKNISQD
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHH
KLEQIIQRLAETDSVMKQGKMEKSLAFELLLMELIVA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]