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The map label for this gene is sigA [H]

Identifier: 23099399

GI number: 23099399

Start: 1969918

End: 1971060

Strand: Reverse

Name: sigA [H]

Synonym: OB1944

Alternate gene names: 23099399

Gene position: 1971060-1969918 (Counterclockwise)

Preceding gene: 23099400

Following gene: 23099398

Centisome position: 54.29

GC content: 39.11

Gene sequence:

>1143_bases
ATGGCCGAACAAAAGCCTTCGCATATGAAAGAAGAAGTAACAGAAGTAACGGAAACAACAGAAGTAACTACATTGGAACA
GGCTAAGAATAGACTTTTAGAAATTGGTAAGAAACGCGGTTCATTAGCCTATGAAGAGGTGGCAGACGGTTTATCTAACT
TTGAGCTTGAACCTGAGCAGATGGACGAATTTTATGAGTACCTAGAAGAACAGGGTGTTGATGTGATAGGTGAGTCGGAA
GATGACCCCGAAATGCAGAAAATCGCAAAAGAAGAGGAATTTAATCTTAATGATTTAAGTGTACCATTAGGTATTAAGAT
AAATGACCCTGTTCGTATGTACTTAAAGGAAATTGGCAGAGTCAATCTATTGTCTGCTGCAGAAGAAATCGATCTTGCTT
CAAGTATTGAAGAAGGCGATGAAGAAGCAAAAAGACGTCTAGCTGAAGCGAACTTACGATTAGTAGTTAGTATAGCAAAA
CGTTATGTTGGACGAGGAATGTTATTCTTGGATCTTATCCAAGAAGGAAATATGGGCCTTATCAAAGCTGTTGAGAAGTT
TGATTATCGTAAAGGGTTTAAATTTAGTACGTATGCCACTTGGTGGATACGTCAGGCAATTACCCGTGCAATAGCTGACC
AAGCAAGAACAATCCGAATTCCAGTTCATATGGTTGAAACGATTAATAAGTTGATTAGAGTTCAACGGTCATTGTTACAA
GATCTAGGACGAGAACCAACACCAGAGGAAATTGGTGAGGAAATGGAATTAGGTCCGGATAAGGTAAGAGATATTCTTAA
GATTGCTCAAGAACCAGTATCATTAGAAACTCCGATTGGAGAAGAAGATGATTCTCATTTAGGCGACTTTATAGAGGACC
AAGAAGCGATTTCCCCGTCAGACCATGCAGCTTATGAATTGCTAAAAGAACAGTTAGAAGATGTTTTAGATACGCTAACT
GATCGAGAAGAAAATGTCCTTCGATTACGATTTGGACTTGACGATGGACGAACTAGAACATTAGAAGAGGTAGGAAAAGT
CTTCGGAGTTACTCGTGAGCGAATTCGACAAATAGAGGCAAAAGCACTCCGTAAATTAAGACACCCTAGTCGAAGCAAGC
GTCTTAAAGACTTTTTAGATTAA

Upstream 100 bases:

>100_bases
TCCAATGGAAGCAGCAAAAATAGCAATGGAAATCATTGAACTACAAAAACAACGTAAGCACATGCACTAAAAATTATTTA
ACTTGGAAGGAGGGGGACTC

Downstream 100 bases:

>100_bases
ATCGAAGTCATTATCTGCCCGTGGTGGATAATGACTTATGTATATCTAAAATGGGGTGATCTGAATTTCAAAAGAAAAAA
CGAAACTTATATTAGATGAA

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MAEQKPSHMKEEVTEVTETTEVTTLEQAKNRLLEIGKKRGSLAYEEVADGLSNFELEPEQMDEFYEYLEEQGVDVIGESE
DDPEMQKIAKEEEFNLNDLSVPLGIKINDPVRMYLKEIGRVNLLSAAEEIDLASSIEEGDEEAKRRLAEANLRLVVSIAK
RYVGRGMLFLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRSLLQ
DLGREPTPEEIGEEMELGPDKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAISPSDHAAYELLKEQLEDVLDTLT
DREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Sequences:

>Translated_380_residues
MAEQKPSHMKEEVTEVTETTEVTTLEQAKNRLLEIGKKRGSLAYEEVADGLSNFELEPEQMDEFYEYLEEQGVDVIGESE
DDPEMQKIAKEEEFNLNDLSVPLGIKINDPVRMYLKEIGRVNLLSAAEEIDLASSIEEGDEEAKRRLAEANLRLVVSIAK
RYVGRGMLFLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRSLLQ
DLGREPTPEEIGEEMELGPDKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAISPSDHAAYELLKEQLEDVLDTLT
DREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD
>Mature_379_residues
AEQKPSHMKEEVTEVTETTEVTTLEQAKNRLLEIGKKRGSLAYEEVADGLSNFELEPEQMDEFYEYLEEQGVDVIGESED
DPEMQKIAKEEEFNLNDLSVPLGIKINDPVRMYLKEIGRVNLLSAAEEIDLASSIEEGDEEAKRRLAEANLRLVVSIAKR
YVGRGMLFLDLIQEGNMGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRSLLQD
LGREPTPEEIGEEMELGPDKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAISPSDHAAYELLKEQLEDVLDTLTD
REENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEAKALRKLRHPSRSKRLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=68.9075630252101, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI1789098, Length=320, Percent_Identity=42.1875, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.1470588235294, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 43586; Mature: 43455

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQKPSHMKEEVTEVTETTEVTTLEQAKNRLLEIGKKRGSLAYEEVADGLSNFELEPEQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCHHH
MDEFYEYLEEQGVDVIGESEDDPEMQKIAKEEEFNLNDLSVPLGIKINDPVRMYLKEIGR
HHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHCC
VNLLSAAEEIDLASSIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHH
IKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRSLLQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHH
DLGREPTPEEIGEEMELGPDKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAISPS
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
DHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KALRKLRHPSRSKRLKDFLD
HHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
AEQKPSHMKEEVTEVTETTEVTTLEQAKNRLLEIGKKRGSLAYEEVADGLSNFELEPEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCCCHHH
MDEFYEYLEEQGVDVIGESEDDPEMQKIAKEEEFNLNDLSVPLGIKINDPVRMYLKEIGR
HHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHCC
VNLLSAAEEIDLASSIEEGDEEAKRRLAEANLRLVVSIAKRYVGRGMLFLDLIQEGNMGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHH
IKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRSLLQ
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHH
DLGREPTPEEIGEEMELGPDKVRDILKIAQEPVSLETPIGEEDDSHLGDFIEDQEAISPS
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
DHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGKVFGVTRERIRQIEA
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KALRKLRHPSRSKRLKDFLD
HHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987246; 3086839; 8969508; 9384377; 2166045; 8363585 [H]