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The map label for this gene is dnaG [H]

Identifier: 23099400

GI number: 23099400

Start: 1971091

End: 1972905

Strand: Reverse

Name: dnaG [H]

Synonym: OB1945

Alternate gene names: 23099400

Gene position: 1972905-1971091 (Counterclockwise)

Preceding gene: 23099401

Following gene: 23099399

Centisome position: 54.34

GC content: 33.61

Gene sequence:

>1815_bases
ATGACCAATCAGATACCAGATGAAATCATTGAACAAGTGAAAAATGCTAATGATATAGTTGATGTTATTGGAGAATATAT
TCAACTGAAGAAACAAGGACGAAATTACTTTGGTCTTTGTCCATTTCATGGAGAGAAGACACCTTCTTTTTCAGTATCCC
AAGAAAAACAAATATTTCATTGTTTTGGATGTGGAAAAGGTGGGAATGTGTTTACTTTCTTGAAAGAAATAGATAGCTTT
ACATTTTATGAAGCGTTACAGTTCTTAGCGGAGAAGCAAGGAATTGAATTGCCACAAACATCCATAAAAAAAGAATCCTT
CTTATCGGAAGAAGACCAACAAGTACTTACAGCGAATGATTGGCTTATGAAGTTGTACCATCATTTATTGAAGTACACGA
AAGACGGTAAAGAAGGATATCAATATTTGAAAGATCGTGGTATTAGTGATGAAGCTTTAGAGGATTTTCAGATTGGCTAT
GCTCCAAATATAAAAGACTTTACTTTAGAGTTCCTTACGAAGAAAGGGTTTAGTAAACAATTACTTGTGAAAAACGGTAT
TTTATCAGCACAGAATGATCATGATGTTATAGACCGTTTTCGTGGTCGCGTGATTTTTCCAATTCGTAATGCAATAGGAA
AAACAGTTGGTTTCGTTGGGCGTGCTCTGCATGATGGTGTAGAACCAAAGTATTTAAATAGCCCTGAGGGCGGCTTGTTT
CAAAAAAGTAAAATATTATTTAATTTTGATTTAGCGAAAAAACACATTCGAAAAGAAAATGAAGTGATATTGTTTGAGGG
TTCTGTTGATGTTATTAGTGCTTACCAAAATGGTTTAAAGAATGTAGTAGCTACATTAGGTACAGCTTTGACGGAATCTC
AAGCTAAGTTATTAAACCGTTATGTGGAAACTGTGGTCCTATGTTATGACGGAGATGATGCAGGTCTTAATGCGGCTTAT
AAAGCTTCTCAGTTACTTCGATCGGTAGGATGTGAAATAAGAATTGCCAAAATGGATCCCTCTGATGACCCGGATAGCTT
TATACGAAGTAATGGTATGGAAGCTTTTCAGAATCGGATAATTCGAACAAGTGATACGTATATGACCTTCTATATGAGAT
ATTTTCGCAGAGATTATAACTTAAAAGTAGAAGGAGATCGAATTGCTTATATTGAAAAAGTACTAAAAGAACTTTCAATG
ATTGAGAGTACCCTAGAACGGGAATATTATGTGCAAGAGTTGCATAAGGAATTTAATTTGTCTCATGAATCTTTGATGAC
TCAATTACAATCATATCGGAATAACCAACAGAATTCTAGGGATAATCATAGGTATAACAGATATACTAATAAAGGTAATT
CATCATACCAAACCAAGCTCCATCCTGCTTTTATTAATGCAGAAAAAAAATTAATTGCATATATGTTACAGGATTCGTAT
ATTACTGAAAAGGTACAAAACGATTTAGGAGCGGCATTTAATGTGAATGATCACAAAATTATTGCTACGCATTTATATGC
TTATTACGAGGAGGGAAATCCTCCTGATATAGGATTATTTATGGAAAGACTATCCGAAGATCATTTGAAAAAACATGTTG
TTGAAGCAGTTATGATGACGGATATTGCAAATAGTACAGATGAAGAAATTGATGATTACATTCGATTTATCTTAAATGAA
AGTAATCAAAATGTAAGTATTAAATCCTTGAAAGAAAAGCAAAGAATTGCTGAGCAAGAAAACAATCCAATGGAAGCAGC
AAAAATAGCAATGGAAATCATTGAACTACAAAAACAACGTAAGCACATGCACTAA

Upstream 100 bases:

>100_bases
GCATTTAGAAGGTGTTTTTTTCGGAATAAGAGAAAAATATGTCGAAAAGTGAAGGATTTATTTAATATGTAACGAATAAT
AGTATAGTATGGTGGTAAAG

Downstream 100 bases:

>100_bases
AAATTATTTAACTTGGAAGGAGGGGGACTCATGGCCGAACAAAAGCCTTCGCATATGAAAGAAGAAGTAACAGAAGTAAC
GGAAACAACAGAAGTAACTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 603

Protein sequence:

>604_residues
MTNQIPDEIIEQVKNANDIVDVIGEYIQLKKQGRNYFGLCPFHGEKTPSFSVSQEKQIFHCFGCGKGGNVFTFLKEIDSF
TFYEALQFLAEKQGIELPQTSIKKESFLSEEDQQVLTANDWLMKLYHHLLKYTKDGKEGYQYLKDRGISDEALEDFQIGY
APNIKDFTLEFLTKKGFSKQLLVKNGILSAQNDHDVIDRFRGRVIFPIRNAIGKTVGFVGRALHDGVEPKYLNSPEGGLF
QKSKILFNFDLAKKHIRKENEVILFEGSVDVISAYQNGLKNVVATLGTALTESQAKLLNRYVETVVLCYDGDDAGLNAAY
KASQLLRSVGCEIRIAKMDPSDDPDSFIRSNGMEAFQNRIIRTSDTYMTFYMRYFRRDYNLKVEGDRIAYIEKVLKELSM
IESTLEREYYVQELHKEFNLSHESLMTQLQSYRNNQQNSRDNHRYNRYTNKGNSSYQTKLHPAFINAEKKLIAYMLQDSY
ITEKVQNDLGAAFNVNDHKIIATHLYAYYEEGNPPDIGLFMERLSEDHLKKHVVEAVMMTDIANSTDEEIDDYIRFILNE
SNQNVSIKSLKEKQRIAEQENNPMEAAKIAMEIIELQKQRKHMH

Sequences:

>Translated_604_residues
MTNQIPDEIIEQVKNANDIVDVIGEYIQLKKQGRNYFGLCPFHGEKTPSFSVSQEKQIFHCFGCGKGGNVFTFLKEIDSF
TFYEALQFLAEKQGIELPQTSIKKESFLSEEDQQVLTANDWLMKLYHHLLKYTKDGKEGYQYLKDRGISDEALEDFQIGY
APNIKDFTLEFLTKKGFSKQLLVKNGILSAQNDHDVIDRFRGRVIFPIRNAIGKTVGFVGRALHDGVEPKYLNSPEGGLF
QKSKILFNFDLAKKHIRKENEVILFEGSVDVISAYQNGLKNVVATLGTALTESQAKLLNRYVETVVLCYDGDDAGLNAAY
KASQLLRSVGCEIRIAKMDPSDDPDSFIRSNGMEAFQNRIIRTSDTYMTFYMRYFRRDYNLKVEGDRIAYIEKVLKELSM
IESTLEREYYVQELHKEFNLSHESLMTQLQSYRNNQQNSRDNHRYNRYTNKGNSSYQTKLHPAFINAEKKLIAYMLQDSY
ITEKVQNDLGAAFNVNDHKIIATHLYAYYEEGNPPDIGLFMERLSEDHLKKHVVEAVMMTDIANSTDEEIDDYIRFILNE
SNQNVSIKSLKEKQRIAEQENNPMEAAKIAMEIIELQKQRKHMH
>Mature_603_residues
TNQIPDEIIEQVKNANDIVDVIGEYIQLKKQGRNYFGLCPFHGEKTPSFSVSQEKQIFHCFGCGKGGNVFTFLKEIDSFT
FYEALQFLAEKQGIELPQTSIKKESFLSEEDQQVLTANDWLMKLYHHLLKYTKDGKEGYQYLKDRGISDEALEDFQIGYA
PNIKDFTLEFLTKKGFSKQLLVKNGILSAQNDHDVIDRFRGRVIFPIRNAIGKTVGFVGRALHDGVEPKYLNSPEGGLFQ
KSKILFNFDLAKKHIRKENEVILFEGSVDVISAYQNGLKNVVATLGTALTESQAKLLNRYVETVVLCYDGDDAGLNAAYK
ASQLLRSVGCEIRIAKMDPSDDPDSFIRSNGMEAFQNRIIRTSDTYMTFYMRYFRRDYNLKVEGDRIAYIEKVLKELSMI
ESTLEREYYVQELHKEFNLSHESLMTQLQSYRNNQQNSRDNHRYNRYTNKGNSSYQTKLHPAFINAEKKLIAYMLQDSYI
TEKVQNDLGAAFNVNDHKIIATHLYAYYEEGNPPDIGLFMERLSEDHLKKHVVEAVMMTDIANSTDEEIDDYIRFILNES
NQNVSIKSLKEKQRIAEQENNPMEAAKIAMEIIELQKQRKHMH

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=455, Percent_Identity=33.6263736263736, Blast_Score=252, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 69723; Mature: 69592

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQIPDEIIEQVKNANDIVDVIGEYIQLKKQGRNYFGLCPFHGEKTPSFSVSQEKQIFH
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHCEEE
CFGCGKGGNVFTFLKEIDSFTFYEALQFLAEKQGIELPQTSIKKESFLSEEDQQVLTAND
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
WLMKLYHHLLKYTKDGKEGYQYLKDRGISDEALEDFQIGYAPNIKDFTLEFLTKKGFSKQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
LLVKNGILSAQNDHDVIDRFRGRVIFPIRNAIGKTVGFVGRALHDGVEPKYLNSPEGGLF
HHHHCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
QKSKILFNFDLAKKHIRKENEVILFEGSVDVISAYQNGLKNVVATLGTALTESQAKLLNR
ECCEEEEEHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVETVVLCYDGDDAGLNAAYKASQLLRSVGCEIRIAKMDPSDDPDSFIRSNGMEAFQNRI
HHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCHHHHHCCC
IRTSDTYMTFYMRYFRRDYNLKVEGDRIAYIEKVLKELSMIESTLEREYYVQELHKEFNL
CCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SHESLMTQLQSYRNNQQNSRDNHRYNRYTNKGNSSYQTKLHPAFINAEKKLIAYMLQDSY
CHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
ITEKVQNDLGAAFNVNDHKIIATHLYAYYEEGNPPDIGLFMERLSEDHLKKHVVEAVMMT
HHHHHHHHCCCEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DIANSTDEEIDDYIRFILNESNQNVSIKSLKEKQRIAEQENNPMEAAKIAMEIIELQKQR
HHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHMH
HCCC
>Mature Secondary Structure 
TNQIPDEIIEQVKNANDIVDVIGEYIQLKKQGRNYFGLCPFHGEKTPSFSVSQEKQIFH
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHCEEE
CFGCGKGGNVFTFLKEIDSFTFYEALQFLAEKQGIELPQTSIKKESFLSEEDQQVLTAND
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHH
WLMKLYHHLLKYTKDGKEGYQYLKDRGISDEALEDFQIGYAPNIKDFTLEFLTKKGFSKQ
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHH
LLVKNGILSAQNDHDVIDRFRGRVIFPIRNAIGKTVGFVGRALHDGVEPKYLNSPEGGLF
HHHHCCCCCCCCCHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
QKSKILFNFDLAKKHIRKENEVILFEGSVDVISAYQNGLKNVVATLGTALTESQAKLLNR
ECCEEEEEHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YVETVVLCYDGDDAGLNAAYKASQLLRSVGCEIRIAKMDPSDDPDSFIRSNGMEAFQNRI
HHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCHHHHHCCC
IRTSDTYMTFYMRYFRRDYNLKVEGDRIAYIEKVLKELSMIESTLEREYYVQELHKEFNL
CCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SHESLMTQLQSYRNNQQNSRDNHRYNRYTNKGNSSYQTKLHPAFINAEKKLIAYMLQDSY
CHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
ITEKVQNDLGAAFNVNDHKIIATHLYAYYEEGNPPDIGLFMERLSEDHLKKHVVEAVMMT
HHHHHHHHCCCEECCCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DIANSTDEEIDDYIRFILNESNQNVSIKSLKEKQRIAEQENNPMEAAKIAMEIIELQKQR
HHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KHMH
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3919021; 3086839; 8969508; 9384377 [H]