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The map label for this gene is spo0A [H]

Identifier: 23099327

GI number: 23099327

Start: 1906010

End: 1906792

Strand: Reverse

Name: spo0A [H]

Synonym: OB1872

Alternate gene names: 23099327

Gene position: 1906792-1906010 (Counterclockwise)

Preceding gene: 23099328

Following gene: 23099325

Centisome position: 52.52

GC content: 34.48

Gene sequence:

>783_bases
GTGGAGAAAATTTCTGTTTGTTTAGTTGATGATAACAAAGAACTTATTGAGATGATGGAGGACTATTTTAGCGAACAACA
TGATATTGAAGTTATTGGAACTGCTTTTAATGGGAGAGATTGTATTAGTATGCTGGAGGAAAAAAATCCAGATGTATTAA
TATTAGATATCATAATGCCCCATATCGATGGGTTAGCTGTATTAAATAGTATTAAATCCATGAATTTAACACCTAAACCA
AATGTTATTATGTTGACAGCATTTGGCCAAGAAGAAGTAATGAAAAAGGCAGTTGATTTAGGAGCCTCTTATTTTATTCT
AAAACCATTCGATTTATCTAATTTAGCAGATCAAATTCGACAGGTTCAAGGCACTACCGTTACTTCTAGTCCTACAGTAT
CAAAAACAGCTAAAGTAGAAAAGAAGAAACAGGATCTTGAGGCAAATATAACGAATATTATTCATGAGATTGGAGTTCCT
GCACATATTAAAGGGTATATGTATTTAAGAGAAGCGATTACAATGGTATATAATGACATTGAATTGCTAGGTTCAATCAC
AAAGGTACTATATCCTGATATAGCGAAGAAATTTAATACCACTGCCTCACGAGTAGAAAGAGCAATACGTCATGCGATTG
AAGTTGCATGGAGCCGGGGCAATATTGATTCTATCTCTGCCTTGTTTGGTTACACCATAAATATTTCGAAAGCTAAGCCA
ACAAACTCTGAATTTATTGCTATGGTAGCTGACCGGTTAAGGTTGGAGCATAAAGTCAGCTAA

Upstream 100 bases:

>100_bases
ATTTAAAAAAATTTAAAGTTTTTTAATTAAAAAGAGGATATTTTATACTTTTGTAGAATATGTAAATTGTGGACCAAAAG
ATAAAGGGAGGAAATAACTG

Downstream 100 bases:

>100_bases
ATCGTTTATATGGTACATACGCACAGTAGGGCGTTAATTGATGTACAAAATAAACCGTCCTTCTTGATTACATGATTTAA
ATCAAGAAGGACGGTTTTGT

Product: stage 0 sporulation protein

Products: protein L-glutamate+ methanol; methanol; Tapglu; methanol; Targlu; Trgglu; Tsrglu; NH3; protein-L-glutamate [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MEKISVCLVDDNKELIEMMEDYFSEQHDIEVIGTAFNGRDCISMLEEKNPDVLILDIIMPHIDGLAVLNSIKSMNLTPKP
NVIMLTAFGQEEVMKKAVDLGASYFILKPFDLSNLADQIRQVQGTTVTSSPTVSKTAKVEKKKQDLEANITNIIHEIGVP
AHIKGYMYLREAITMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISALFGYTINISKAKP
TNSEFIAMVADRLRLEHKVS

Sequences:

>Translated_260_residues
MEKISVCLVDDNKELIEMMEDYFSEQHDIEVIGTAFNGRDCISMLEEKNPDVLILDIIMPHIDGLAVLNSIKSMNLTPKP
NVIMLTAFGQEEVMKKAVDLGASYFILKPFDLSNLADQIRQVQGTTVTSSPTVSKTAKVEKKKQDLEANITNIIHEIGVP
AHIKGYMYLREAITMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISALFGYTINISKAKP
TNSEFIAMVADRLRLEHKVS
>Mature_260_residues
MEKISVCLVDDNKELIEMMEDYFSEQHDIEVIGTAFNGRDCISMLEEKNPDVLILDIIMPHIDGLAVLNSIKSMNLTPKP
NVIMLTAFGQEEVMKKAVDLGASYFILKPFDLSNLADQIRQVQGTTVTSSPTVSKTAKVEKKKQDLEANITNIIHEIGVP
AHIKGYMYLREAITMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISALFGYTINISKAKP
TNSEFIAMVADRLRLEHKVS

Specific function: May play the central regulatory role in sporulation. It may be an element of the effector pathway responsible for the activation of sporulation genes in response to nutritional stress. Spo0A may act in concert with Spo0H (a sigma factor) to control the ex

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788192, Length=110, Percent_Identity=37.2727272727273, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082052, Length=112, Percent_Identity=35.7142857142857, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=34.5454545454545, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR014879
- InterPro:   IPR012052
- InterPro:   IPR011991
- ProDom:   PD010908 [H]

Pfam domain/function: PF00072 Response_reg; PF08769 Spo0A_C [H]

EC number: 3.1.1.61 [C]

Molecular weight: Translated: 29104; Mature: 29104

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISVCLVDDNKELIEMMEDYFSEQHDIEVIGTAFNGRDCISMLEEKNPDVLILDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEHHH
HIDGLAVLNSIKSMNLTPKPNVIMLTAFGQEEVMKKAVDLGASYFILKPFDLSNLADQIR
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
QVQGTTVTSSPTVSKTAKVEKKKQDLEANITNIIHEIGVPAHIKGYMYLREAITMVYNDI
HHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISALFGYTINISKAKP
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCCCC
TNSEFIAMVADRLRLEHKVS
CCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKISVCLVDDNKELIEMMEDYFSEQHDIEVIGTAFNGRDCISMLEEKNPDVLILDIIMP
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEHHH
HIDGLAVLNSIKSMNLTPKPNVIMLTAFGQEEVMKKAVDLGASYFILKPFDLSNLADQIR
CCCHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHH
QVQGTTVTSSPTVSKTAKVEKKKQDLEANITNIIHEIGVPAHIKGYMYLREAITMVYNDI
HHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISALFGYTINISKAKP
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCEEEEECCCCC
TNSEFIAMVADRLRLEHKVS
CCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein L-glutamate Omethyl ester; Tapglu-Me; H2O; Targlu-Me; Trgglu-Me; Tsrglu-Me; Tsrgln; Trggln; Targln; Tapgln; protein-L-glutamine [C]

Specific reaction: Protein L-glutamate Omethyl ester + H2O = protein L-glutamate+ methanol H2O + Tapglu-Me = methanol + Tapglu H2O + Targlu-Me = methanol + Targlu H2O + Trgglu-Me = methanol + Trgglu H2O + Tsrglu-Me = methanol + Tsrglu H2O + Tsrgln = NH3 + Tsrglu H2O + Trggl

General reaction: Hydrolysis of carboxylic ester [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629; 7885226 [H]