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The map label for this gene is bfmBB [H]

Identifier: 23099319

GI number: 23099319

Start: 1894784

End: 1896067

Strand: Reverse

Name: bfmBB [H]

Synonym: OB1864

Alternate gene names: 23099319

Gene position: 1896067-1894784 (Counterclockwise)

Preceding gene: 23099320

Following gene: 23099318

Centisome position: 52.23

GC content: 36.14

Gene sequence:

>1284_bases
ATGTCAGTAGAAAAAATTAACATGCCTCAGTTAGGAGAAAGTGTTACAGAAGGTACGATAAACACTTGGCTCGTAGCGGT
AGGTGATAAAGTAAATAAATATGATCCCATAGCTGAAGTGATGACGGATAAAGTGAATGCAGAAGTGCCTTCATCGTTTT
CTGGTGTGATTAAGGAATTGATTGCCGAAGAAGGAGAAACCGTAGAAGTCGGTCAGTTAATGTGCTACATCGATACGGAA
GAATCAAGTTCAGCAAGTAAACCGAAAAGTTCTGAAGATAGCACGAAAACGAACGATAACATAGAAAACGATACTAATTC
GAAAGAAGACGTCAACAAATCAATGAAAAAAAGGTACTCACCAGCAGTACTTCGATTAGCTCAGGAAAATGATATTGATT
TAACAATGATAGATGGTTCCGGTCGTGCTGGAAGGATTACAAGAAAAGATGTAGAGAAATTTATGAAAGAAGGTACAAAA
AATGATCCTAAAGAAGAGCAAAAAGAACCTTCTCATCAAACAATACCTAAAGTGAATCAGAGCGGAACACCGTCTGTGCG
TTCTAATTCTATTGCAAGTGAAAAGGGAGATAAAGAGATACCTGTAAAAGGGGTTCGAAAAGCGATTGCAGATAATATGG
TTCGTTCAAAACAAGAAATACCTCATGCATGGATGCAAGTAGAAGTAGATGTAACCAACATGATGAAGTATCGTAATTCT
ATCAAGGACAAATTTAAGTCTGATGAAGGATATAGTTTGACGCCATTTGCTTTCTTTATAAAAGCTGTTTCCCAAGCTTT
AAGAGAATATCCGGAGTTAAACAGTGCTTGGGCAGGAGATAAAATAATTCAGAAAAAAGATATTCATTTATCTATAGCTG
TAGCAAAAGAAAATGAATTATTTGTACCAGTAATAAGGCATGCAGATGAAAAAACAATAAAAGCTGTTGCTCGTGATATA
TATGAACTTGCAAACAAAGCAAGAAATGGAAAATTATCATCTGAAGATATGCAAGGTGGAACATTTACGGTAAATAATAC
TGGCACGTTTGGATCGGTTTCTTCTATGGGAGTAATTAATCATCCACAAGCAGCCATATTACAAGTAGAATCGATTGTAC
GCAAACCAGTTATTATAGATGACATGTTTGCTGCAAGGGACATGGCTAATCTTTCTTTATCTTTAGATCACCGCATTTTA
GATGGATTAGTATGTGGCAGATTCCTTGCGAGAGTCAAAGAAATTCTAGAAGGTATGAATGTAGATACGGTCTCACTTTA
TTAA

Upstream 100 bases:

>100_bases
TGCCTTATGCGCCAACAATGGAGAAGTACTTTATGGTTAATCCAGATAAAGTAGAAAAAGCAATGCGAGAACTCGCAGAA
TACTAATTGGAGGTATATAG

Downstream 100 bases:

>100_bases
GATATTGAATAAGAAATTAATAAATTGAAATTTTTGGGTGATGCTGATAAGCTAGACCTAAATACATTTCATATTAAAAG
GATGGGTTATCATGGATTTC

Product: branched-chain alpha-keto acid dehydrogenase E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSVEKINMPQLGESVTEGTINTWLVAVGDKVNKYDPIAEVMTDKVNAEVPSSFSGVIKELIAEEGETVEVGQLMCYIDTE
ESSSASKPKSSEDSTKTNDNIENDTNSKEDVNKSMKKRYSPAVLRLAQENDIDLTMIDGSGRAGRITRKDVEKFMKEGTK
NDPKEEQKEPSHQTIPKVNQSGTPSVRSNSIASEKGDKEIPVKGVRKAIADNMVRSKQEIPHAWMQVEVDVTNMMKYRNS
IKDKFKSDEGYSLTPFAFFIKAVSQALREYPELNSAWAGDKIIQKKDIHLSIAVAKENELFVPVIRHADEKTIKAVARDI
YELANKARNGKLSSEDMQGGTFTVNNTGTFGSVSSMGVINHPQAAILQVESIVRKPVIIDDMFAARDMANLSLSLDHRIL
DGLVCGRFLARVKEILEGMNVDTVSLY

Sequences:

>Translated_427_residues
MSVEKINMPQLGESVTEGTINTWLVAVGDKVNKYDPIAEVMTDKVNAEVPSSFSGVIKELIAEEGETVEVGQLMCYIDTE
ESSSASKPKSSEDSTKTNDNIENDTNSKEDVNKSMKKRYSPAVLRLAQENDIDLTMIDGSGRAGRITRKDVEKFMKEGTK
NDPKEEQKEPSHQTIPKVNQSGTPSVRSNSIASEKGDKEIPVKGVRKAIADNMVRSKQEIPHAWMQVEVDVTNMMKYRNS
IKDKFKSDEGYSLTPFAFFIKAVSQALREYPELNSAWAGDKIIQKKDIHLSIAVAKENELFVPVIRHADEKTIKAVARDI
YELANKARNGKLSSEDMQGGTFTVNNTGTFGSVSSMGVINHPQAAILQVESIVRKPVIIDDMFAARDMANLSLSLDHRIL
DGLVCGRFLARVKEILEGMNVDTVSLY
>Mature_426_residues
SVEKINMPQLGESVTEGTINTWLVAVGDKVNKYDPIAEVMTDKVNAEVPSSFSGVIKELIAEEGETVEVGQLMCYIDTEE
SSSASKPKSSEDSTKTNDNIENDTNSKEDVNKSMKKRYSPAVLRLAQENDIDLTMIDGSGRAGRITRKDVEKFMKEGTKN
DPKEEQKEPSHQTIPKVNQSGTPSVRSNSIASEKGDKEIPVKGVRKAIADNMVRSKQEIPHAWMQVEVDVTNMMKYRNSI
KDKFKSDEGYSLTPFAFFIKAVSQALREYPELNSAWAGDKIIQKKDIHLSIAVAKENELFVPVIRHADEKTIKAVARDIY
ELANKARNGKLSSEDMQGGTFTVNNTGTFGSVSSMGVINHPQAAILQVESIVRKPVIIDDMFAARDMANLSLSLDHRILD
GLVCGRFLARVKEILEGMNVDTVSLY

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=28.6363636363636, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI110671329, Length=419, Percent_Identity=30.7875894988067, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=28.7610619469027, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=28.7610619469027, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=33.7719298245614, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=35.2564102564103, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1786946, Length=417, Percent_Identity=32.3741007194245, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=30.0925925925926, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=430, Percent_Identity=27.4418604651163, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17560088, Length=424, Percent_Identity=27.122641509434, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=27.0731707317073, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=304, Percent_Identity=30.5921052631579, Blast_Score=124, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=30.0715990453461, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=25.1121076233184, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=30.2816901408451, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI20129315, Length=223, Percent_Identity=34.5291479820628, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24582497, Length=223, Percent_Identity=34.5291479820628, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24645909, Length=234, Percent_Identity=31.1965811965812, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 47311; Mature: 47180

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEKINMPQLGESVTEGTINTWLVAVGDKVNKYDPIAEVMTDKVNAEVPSSFSGVIKEL
CCCCCCCCCHHCCHHHCCCHHHEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
IAEEGETVEVGQLMCYIDTEESSSASKPKSSEDSTKTNDNIENDTNSKEDVNKSMKKRYS
HHCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PAVLRLAQENDIDLTMIDGSGRAGRITRKDVEKFMKEGTKNDPKEEQKEPSHQTIPKVNQ
HHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCC
SGTPSVRSNSIASEKGDKEIPVKGVRKAIADNMVRSKQEIPHAWMQVEVDVTNMMKYRNS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
IKDKFKSDEGYSLTPFAFFIKAVSQALREYPELNSAWAGDKIIQKKDIHLSIAVAKENEL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEECCCCE
FVPVIRHADEKTIKAVARDIYELANKARNGKLSSEDMQGGTFTVNNTGTFGSVSSMGVIN
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
HPQAAILQVESIVRKPVIIDDMFAARDMANLSLSLDHRILDGLVCGRFLARVKEILEGMN
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDTVSLY
CCEEEEC
>Mature Secondary Structure 
SVEKINMPQLGESVTEGTINTWLVAVGDKVNKYDPIAEVMTDKVNAEVPSSFSGVIKEL
CCCCCCCCHHCCHHHCCCHHHEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHH
IAEEGETVEVGQLMCYIDTEESSSASKPKSSEDSTKTNDNIENDTNSKEDVNKSMKKRYS
HHCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PAVLRLAQENDIDLTMIDGSGRAGRITRKDVEKFMKEGTKNDPKEEQKEPSHQTIPKVNQ
HHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCC
SGTPSVRSNSIASEKGDKEIPVKGVRKAIADNMVRSKQEIPHAWMQVEVDVTNMMKYRNS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
IKDKFKSDEGYSLTPFAFFIKAVSQALREYPELNSAWAGDKIIQKKDIHLSIAVAKENEL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEECCCEEEEEEECCCCE
FVPVIRHADEKTIKAVARDIYELANKARNGKLSSEDMQGGTFTVNNTGTFGSVSSMGVIN
EEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
HPQAAILQVESIVRKPVIIDDMFAARDMANLSLSLDHRILDGLVCGRFLARVKEILEGMN
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
VDTVSLY
CCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]