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The map label for this gene is sigF [H]

Identifier: 23099294

GI number: 23099294

Start: 1871926

End: 1872678

Strand: Reverse

Name: sigF [H]

Synonym: OB1839

Alternate gene names: 23099294

Gene position: 1872678-1871926 (Counterclockwise)

Preceding gene: 23099295

Following gene: 23099291

Centisome position: 51.58

GC content: 35.86

Gene sequence:

>753_bases
ATGAATGTGAATAAAACACGGGAGCGGGAGCAGCTTTCCGATGAGCAGGTAAAAGAACTTATACGTTTGAGCCAGCAAGG
GGACCAATCAGCAAGAGACCGTTTAGTAGAGAAAAATATACGACTTGTATGGTCAGTAGTTCAACGATTTATTAATCGGG
GGTACGATCCGGATGATTTATTTCAAATTGGAAGTATTGGTTTAATAAAATCAATTGATAAATTTGATTTAAGTTATGAT
GTTAAATTTTCCACCTATGCTGTACCGATGATTATCGGAGAGATTCAGCGTTTTATTCGTGATGATGGCAGTATTAAAGT
AAGTAGATCTTTGAAAGAAATAGGAAATAAAATAAGAAGAAAAAAAGATGAAATGACGAAACAATATGGGCGTTCGCCAA
CTGTGAACGAATTAGCAGAAGCGCTTGAATTACAGCCGGAAGAAGTTGTTCATGCGCAAGAAGCAGCAAAGTCACCACAA
TCGATCTATGAGACCGTGTTTGAAAATGATGGTGATCCAATAACATTGTTAGATCAGATCGCAGATCAAGATTCAAAGTG
GTTTGAAAAACTATCATTACAAGAAGCAATTCGAAGTTTGACGGAAAGAGAGCGTCTAATCATATATCTTCGTTATTATA
AGGATAAAACACAAACAGAGGTAGCAGAAAGATTAGGAATTTCACAGGTACAAGTATCACGACTAGAGAAAAAGATTTTA
GAGGATATGAAAAAAAATATGGATACAAGTTAG

Upstream 100 bases:

>100_bases
GGATTCTGTGGAAGTTATTTCTGCTCCCGAAAAAGGTACTTCCGTATATATGACCAAACAACTTTCGAAATCAAAAACTG
TTTATAATTAAGGTCTAATC

Downstream 100 bases:

>100_bases
CACGTAAAGAGCCTCCTTTTTGGGAGGCTCTCAGGCTGTCGAGAAACCCTCGGCAGTCTATTTTTATGTGGGAAACAGCC
CCAATTGACCGTAATTATTT

Product: sporulation sigma factor SigF

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MNVNKTREREQLSDEQVKELIRLSQQGDQSARDRLVEKNIRLVWSVVQRFINRGYDPDDLFQIGSIGLIKSIDKFDLSYD
VKFSTYAVPMIIGEIQRFIRDDGSIKVSRSLKEIGNKIRRKKDEMTKQYGRSPTVNELAEALELQPEEVVHAQEAAKSPQ
SIYETVFENDGDPITLLDQIADQDSKWFEKLSLQEAIRSLTERERLIIYLRYYKDKTQTEVAERLGISQVQVSRLEKKIL
EDMKKNMDTS

Sequences:

>Translated_250_residues
MNVNKTREREQLSDEQVKELIRLSQQGDQSARDRLVEKNIRLVWSVVQRFINRGYDPDDLFQIGSIGLIKSIDKFDLSYD
VKFSTYAVPMIIGEIQRFIRDDGSIKVSRSLKEIGNKIRRKKDEMTKQYGRSPTVNELAEALELQPEEVVHAQEAAKSPQ
SIYETVFENDGDPITLLDQIADQDSKWFEKLSLQEAIRSLTERERLIIYLRYYKDKTQTEVAERLGISQVQVSRLEKKIL
EDMKKNMDTS
>Mature_250_residues
MNVNKTREREQLSDEQVKELIRLSQQGDQSARDRLVEKNIRLVWSVVQRFINRGYDPDDLFQIGSIGLIKSIDKFDLSYD
VKFSTYAVPMIIGEIQRFIRDDGSIKVSRSLKEIGNKIRRKKDEMTKQYGRSPTVNELAEALELQPEEVVHAQEAAKSPQ
SIYETVFENDGDPITLLDQIADQDSKWFEKLSLQEAIRSLTERERLIIYLRYYKDKTQTEVAERLGISQVQVSRLEKKIL
EDMKKNMDTS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=33.201581027668, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789098, Length=251, Percent_Identity=25.8964143426295, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=25.8706467661692, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 29136; Mature: 29136

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCC
>Mature Secondary Structure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HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2513372 [H]