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The map label for this gene is asnC

Identifier: 23099214

GI number: 23099214

Start: 1797807

End: 1799099

Strand: Reverse

Name: asnC

Synonym: OB1759

Alternate gene names: 23099214

Gene position: 1799099-1797807 (Counterclockwise)

Preceding gene: 23099215

Following gene: 23099213

Centisome position: 49.55

GC content: 35.96

Gene sequence:

>1293_bases
ATGAAAATTACAATCGCACAGGCACCACAATATATAGAACAAGAAGTAACCATTGGTGCATGGTTATCTAACAAACGCTC
TAGCGGTAAAATAGCTTTTTTACAATTACGTGATGGAACAGGATTTATGCAAGGGATAGTAGTCAAAAGTGATGTAAGTG
AAGATGTTTTTTCAACATCAAAAAACATTACACAAGAATCATCTCTATACGTAACTGGCAAAATTGTTGAAGATACACGA
TCTGATTTTGGATACGAAATGCAAGTTAGCAATGTTGAAGTGATTCATGAAGCTAATGACTATCCAATTACGCCTAAAGA
ACATGGAACAGAATTTTTAATGGATCATCGTCATCTGTGGTTACGTTCCAAAAAACAACATGCAGTAATGAAAATCAGAA
ATGAAATTATCCGTTCTACGTATGAGTATTTTAATAAAGAGGGATTTGTAAAAGTAGATCCACCGATACTTACTGGATCT
TCTGCAGAAGGAACTACAGAGCTATTCCATACAAAGTATTTTGATGAAGAAGCATATCTTTCACAAAGTGGACAACTTTA
CATGGAAGCAGCAGCAATGGCATTAGGAAAAGTATTTTCATTCGGTCCAACTTTCCGAGCGGAAAAATCAAAAACAAGAA
GACATCTTATCGAATTTTGGATGATTGAACCAGAGATGGCATTTGTTGAACACGAAGAAAGCTTACAAATTCAAGAAAAC
TACGTTAGCCATATCGTACAATCTGTATTAACAAATTGTAAATTAGAGCTATCTGTTCTTGACAGAGATCTATCTAAATT
AGAGAAGATAAAAGCTCCATTTCCGAGAATTTCATATGATGAAGCAATTGATATGTTAAAAGAAAAAGGCTTTGATGATA
TTGAATGGGGAGAAGATTTTGGCGCACCACATGAAACTGCAATCGCGGAAAGCTATGATATGCCAGTCTTTATAACGAAT
TACCCGAAAGATATAAAAGCTTTTTATATGAAACCACATCCTGATCGATCTGATGTTGTTTTATGTGCTGATCTAATTGC
TCCAGAAGGTTATGGTGAAATTATAGGTGGTTCCCAACGAATTGATGATTTAGAACTTATGGAACAGCGTTACGAAGAAC
ATGGATTAACCGGACCAGCTTATCAGTGGTATTTAGAACTACGTAAGTATGGAAGTGTACCACACTCTGGATTTGGATTA
GGATTAGAGCGTACAGTAGCTTGGTTATCTGGAGTGGAGCATGTTCGTGAGACAATCCCATTCCCACGTCTGCTAAATCG
TCTATATCCATAA

Upstream 100 bases:

>100_bases
ATGTACGTTTATCCTATGCACTGTCAATTGAGGAACTAACAGAAGCAGCTAATCGAATTCATCGATTTGTTCTAAATCAT
AAATAATGGAGGACTAAACC

Downstream 100 bases:

>100_bases
TATGAAGGAATCAATCCCTTGCATTTTACGCGAGGGATTTTTCTTTCTATACTACATGTACATGCTATACTGTTATTGAG
GTGCTTATCAATGAAAAAGA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKITIAQAPQYIEQEVTIGAWLSNKRSSGKIAFLQLRDGTGFMQGIVVKSDVSEDVFSTSKNITQESSLYVTGKIVEDTR
SDFGYEMQVSNVEVIHEANDYPITPKEHGTEFLMDHRHLWLRSKKQHAVMKIRNEIIRSTYEYFNKEGFVKVDPPILTGS
SAEGTTELFHTKYFDEEAYLSQSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVEHEESLQIQEN
YVSHIVQSVLTNCKLELSVLDRDLSKLEKIKAPFPRISYDEAIDMLKEKGFDDIEWGEDFGAPHETAIAESYDMPVFITN
YPKDIKAFYMKPHPDRSDVVLCADLIAPEGYGEIIGGSQRIDDLELMEQRYEEHGLTGPAYQWYLELRKYGSVPHSGFGL
GLERTVAWLSGVEHVRETIPFPRLLNRLYP

Sequences:

>Translated_430_residues
MKITIAQAPQYIEQEVTIGAWLSNKRSSGKIAFLQLRDGTGFMQGIVVKSDVSEDVFSTSKNITQESSLYVTGKIVEDTR
SDFGYEMQVSNVEVIHEANDYPITPKEHGTEFLMDHRHLWLRSKKQHAVMKIRNEIIRSTYEYFNKEGFVKVDPPILTGS
SAEGTTELFHTKYFDEEAYLSQSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVEHEESLQIQEN
YVSHIVQSVLTNCKLELSVLDRDLSKLEKIKAPFPRISYDEAIDMLKEKGFDDIEWGEDFGAPHETAIAESYDMPVFITN
YPKDIKAFYMKPHPDRSDVVLCADLIAPEGYGEIIGGSQRIDDLELMEQRYEEHGLTGPAYQWYLELRKYGSVPHSGFGL
GLERTVAWLSGVEHVRETIPFPRLLNRLYP
>Mature_430_residues
MKITIAQAPQYIEQEVTIGAWLSNKRSSGKIAFLQLRDGTGFMQGIVVKSDVSEDVFSTSKNITQESSLYVTGKIVEDTR
SDFGYEMQVSNVEVIHEANDYPITPKEHGTEFLMDHRHLWLRSKKQHAVMKIRNEIIRSTYEYFNKEGFVKVDPPILTGS
SAEGTTELFHTKYFDEEAYLSQSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVEHEESLQIQEN
YVSHIVQSVLTNCKLELSVLDRDLSKLEKIKAPFPRISYDEAIDMLKEKGFDDIEWGEDFGAPHETAIAESYDMPVFITN
YPKDIKAFYMKPHPDRSDVVLCADLIAPEGYGEIIGGSQRIDDLELMEQRYEEHGLTGPAYQWYLELRKYGSVPHSGFGL
GLERTVAWLSGVEHVRETIPFPRLLNRLYP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=437, Percent_Identity=34.0961098398169, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI4758762, Length=443, Percent_Identity=32.2799097065463, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI45439306, Length=457, Percent_Identity=30.6345733041575, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1787161, Length=457, Percent_Identity=38.074398249453, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789256, Length=457, Percent_Identity=23.4135667396061, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790571, Length=457, Percent_Identity=22.7571115973742, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788173, Length=246, Percent_Identity=23.9837398373984, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=440, Percent_Identity=34.5454545454545, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17556372, Length=409, Percent_Identity=32.0293398533007, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17551876, Length=463, Percent_Identity=27.8617710583153, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI32566633, Length=286, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=475, Percent_Identity=21.6842105263158, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=475, Percent_Identity=21.6842105263158, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17535927, Length=475, Percent_Identity=21.6842105263158, Blast_Score=65, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321807, Length=443, Percent_Identity=34.9887133182844, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319872, Length=453, Percent_Identity=32.0088300220751, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323011, Length=462, Percent_Identity=29.2207792207792, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325153, Length=243, Percent_Identity=25.1028806584362, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19921528, Length=440, Percent_Identity=34.0909090909091, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI19922416, Length=439, Percent_Identity=31.4350797266515, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI17136276, Length=439, Percent_Identity=29.1571753986333, Blast_Score=165, Evalue=6e-41,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_OCEIH (Q8EQE2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692680.1
- ProteinModelPortal:   Q8EQE2
- SMR:   Q8EQE2
- GeneID:   1018253
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1759
- NMPDR:   fig|221109.1.peg.1760
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   OIHE221109:OB1759-MONOMER
- BRENDA:   6.1.1.22
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 49286; Mature: 49286

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITIAQAPQYIEQEVTIGAWLSNKRSSGKIAFLQLRDGTGFMQGIVVKSDVSEDVFSTS
CEEEECCCCHHHHHHHEEEHHHCCCCCCCCEEEEEEECCCCCHHEEEEECCCCHHHHHHC
KNITQESSLYVTGKIVEDTRSDFGYEMQVSNVEVIHEANDYPITPKEHGTEFLMDHRHLW
CCCCCCCCEEEEEEEHHHHHCCCCEEEEECCEEEEECCCCCCCCCHHCCHHHHHHHHHHH
LRSKKQHAVMKIRNEIIRSTYEYFNKEGFVKVDPPILTGSSAEGTTELFHTKYFDEEAYL
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCHHHHHHHHHCCCHHHH
SQSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVEHEESLQIQEN
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCHHHHHCCCHHHHHHHH
YVSHIVQSVLTNCKLELSVLDRDLSKLEKIKAPFPRISYDEAIDMLKEKGFDDIEWGEDF
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHC
GAPHETAIAESYDMPVFITNYPKDIKAFYMKPHPDRSDVVLCADLIAPEGYGEIIGGSQR
CCCCHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCEEEEEHHHCCCCCCHHCCCCCC
IDDLELMEQRYEEHGLTGPAYQWYLELRKYGSVPHSGFGLGLERTVAWLSGVEHVRETIP
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
FPRLLNRLYP
HHHHHHHHCC
>Mature Secondary Structure
MKITIAQAPQYIEQEVTIGAWLSNKRSSGKIAFLQLRDGTGFMQGIVVKSDVSEDVFSTS
CEEEECCCCHHHHHHHEEEHHHCCCCCCCCEEEEEEECCCCCHHEEEEECCCCHHHHHHC
KNITQESSLYVTGKIVEDTRSDFGYEMQVSNVEVIHEANDYPITPKEHGTEFLMDHRHLW
CCCCCCCCEEEEEEEHHHHHCCCCEEEEECCEEEEECCCCCCCCCHHCCHHHHHHHHHHH
LRSKKQHAVMKIRNEIIRSTYEYFNKEGFVKVDPPILTGSSAEGTTELFHTKYFDEEAYL
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEECCCCCCHHHHHHHHHCCCHHHH
SQSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVEHEESLQIQEN
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHEECCHHHHHCCCHHHHHHHH
YVSHIVQSVLTNCKLELSVLDRDLSKLEKIKAPFPRISYDEAIDMLKEKGFDDIEWGEDF
HHHHHHHHHHHCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHC
GAPHETAIAESYDMPVFITNYPKDIKAFYMKPHPDRSDVVLCADLIAPEGYGEIIGGSQR
CCCCHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCEEEEEHHHCCCCCCHHCCCCCC
IDDLELMEQRYEEHGLTGPAYQWYLELRKYGSVPHSGFGLGLERTVAWLSGVEHVRETIP
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
FPRLLNRLYP
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376