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The map label for this gene is dapA

Identifier: 23099163

GI number: 23099163

Start: 1753141

End: 1754028

Strand: Direct

Name: dapA

Synonym: OB1708

Alternate gene names: 23099163

Gene position: 1753141-1754028 (Clockwise)

Preceding gene: 23099160

Following gene: 23099164

Centisome position: 48.29

GC content: 36.71

Gene sequence:

>888_bases
ATGAATTTTGGAAAAGTATTAACAGCGATGGTGACGCCTTTCGATAAACATGAAGAAATCGATTTTCAAGCATTGGATCA
TTTAATTGACCATCTAATTAATAATGGGTCAGATGGTTTGGTAGTAGCTGGTACCACAGGTGAATCTCCAACGTTAACAC
ATGAAGAGAGAATAAAATTATTCGAATATGTCGTGAAAAAAGTAAATGGAGCAGTACCCGTAATTGCCGGAACGGGCTCT
AATTATACGAAAGCATCGATTGAATTGACAAAAGAAGTAGCAAAAACAGGTGTTGATGGAGTAATGCTTGTTGCACCATA
TTATAATAAACCGTCACAGGATAGCATGTATGAACATTTTAAGGTAATTGCAGAAAGCACAGAATTACCAGTAATGCTTT
ATAATGTTCCAGGACGCACAGGTGTTGATATGGATGTATCTACTGTTGTACGTTTATCTCAAATAGATAATATTGTGTCC
ATCAAAGATGCAAGCGGGAATTTAGAAAAAATGACAAATATTATTCGCCTTACAGATGATAATTTTTCTGTGTATAGTGG
GGAAGACAGCTTAACTTTACCAGCACTAGCTATCGGTGCTGATGGCATCGTTTCTGTATCCTCTCATATTATTGGGAATG
ATATGCAAGAAATGATTCAACGTTTTGAACAAGGTCAAGTAGCAGAAGCAGCTAATATACATCAGCGAGTACTACCTGTC
ATGCAAGAAATGTTTGCAAATCCAAGTCCTGTTCCAGTTAAAACAGCACTGAATCTTCAATCTGTGCCTGTGGGAAAGGT
TCGATTACCATTAATCGAGCTAACCGATGAACAGTTGCAAAATTTACAGTCCGTATTATCGGATTTCGAACATTCTAAAA
TGATATAA

Upstream 100 bases:

>100_bases
ACTTATATGTTCAGCAGCAACGGGCTTCCGTTGGTGCTTTTTAATTTGGCCCAACCCTCTTAAAGCAAGTTTGTTCAAAT
TATAAAAAGGGTGTGTTTAC

Downstream 100 bases:

>100_bases
TATAAAAATTAAGAAGTCCATGAACATTATATTTTGTGTTTATGGGCTTTTTTTTATTACCTATTGACGGTTTCCGTCAC
AATAATTTATCCAGAATTGT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNFGKVLTAMVTPFDKHEEIDFQALDHLIDHLINNGSDGLVVAGTTGESPTLTHEERIKLFEYVVKKVNGAVPVIAGTGS
NYTKASIELTKEVAKTGVDGVMLVAPYYNKPSQDSMYEHFKVIAESTELPVMLYNVPGRTGVDMDVSTVVRLSQIDNIVS
IKDASGNLEKMTNIIRLTDDNFSVYSGEDSLTLPALAIGADGIVSVSSHIIGNDMQEMIQRFEQGQVAEAANIHQRVLPV
MQEMFANPSPVPVKTALNLQSVPVGKVRLPLIELTDEQLQNLQSVLSDFEHSKMI

Sequences:

>Translated_295_residues
MNFGKVLTAMVTPFDKHEEIDFQALDHLIDHLINNGSDGLVVAGTTGESPTLTHEERIKLFEYVVKKVNGAVPVIAGTGS
NYTKASIELTKEVAKTGVDGVMLVAPYYNKPSQDSMYEHFKVIAESTELPVMLYNVPGRTGVDMDVSTVVRLSQIDNIVS
IKDASGNLEKMTNIIRLTDDNFSVYSGEDSLTLPALAIGADGIVSVSSHIIGNDMQEMIQRFEQGQVAEAANIHQRVLPV
MQEMFANPSPVPVKTALNLQSVPVGKVRLPLIELTDEQLQNLQSVLSDFEHSKMI
>Mature_295_residues
MNFGKVLTAMVTPFDKHEEIDFQALDHLIDHLINNGSDGLVVAGTTGESPTLTHEERIKLFEYVVKKVNGAVPVIAGTGS
NYTKASIELTKEVAKTGVDGVMLVAPYYNKPSQDSMYEHFKVIAESTELPVMLYNVPGRTGVDMDVSTVVRLSQIDNIVS
IKDASGNLEKMTNIIRLTDDNFSVYSGEDSLTLPALAIGADGIVSVSSHIIGNDMQEMIQRFEQGQVAEAANIHQRVLPV
MQEMFANPSPVPVKTALNLQSVPVGKVRLPLIELTDEQLQNLQSVLSDFEHSKMI

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=284, Percent_Identity=30.2816901408451, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI13540533, Length=295, Percent_Identity=27.7966101694915, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI1788823, Length=285, Percent_Identity=40, Blast_Score=237, Evalue=7e-64,
Organism=Escherichia coli, GI87082415, Length=293, Percent_Identity=30.7167235494881, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786463, Length=281, Percent_Identity=32.3843416370107, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789620, Length=288, Percent_Identity=28.125, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_OCEIH (Q8EQJ1)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692629.1
- ProteinModelPortal:   Q8EQJ1
- SMR:   Q8EQJ1
- GeneID:   1018204
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1708
- NMPDR:   fig|221109.1.peg.1711
- HOGENOM:   HBG358848
- OMA:   QKGLIEH
- ProtClustDB:   PRK03170
- BioCyc:   OIHE221109:OB1708-MONOMER
- BRENDA:   4.2.1.52
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 32253; Mature: 32253

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 162-162 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGKVLTAMVTPFDKHEEIDFQALDHLIDHLINNGSDGLVVAGTTGESPTLTHEERIKL
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHH
FEYVVKKVNGAVPVIAGTGSNYTKASIELTKEVAKTGVDGVMLVAPYYNKPSQDSMYEHF
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
KVIAESTELPVMLYNVPGRTGVDMDVSTVVRLSQIDNIVSIKDASGNLEKMTNIIRLTDD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHEEEEECC
NFSVYSGEDSLTLPALAIGADGIVSVSSHIIGNDMQEMIQRFEQGQVAEAANIHQRVLPV
CEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MQEMFANPSPVPVKTALNLQSVPVGKVRLPLIELTDEQLQNLQSVLSDFEHSKMI
HHHHHCCCCCCCEEEECCCCCCCCCCEEECCEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFGKVLTAMVTPFDKHEEIDFQALDHLIDHLINNGSDGLVVAGTTGESPTLTHEERIKL
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHH
FEYVVKKVNGAVPVIAGTGSNYTKASIELTKEVAKTGVDGVMLVAPYYNKPSQDSMYEHF
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
KVIAESTELPVMLYNVPGRTGVDMDVSTVVRLSQIDNIVSIKDASGNLEKMTNIIRLTDD
HHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHHHEEEEECC
NFSVYSGEDSLTLPALAIGADGIVSVSSHIIGNDMQEMIQRFEQGQVAEAANIHQRVLPV
CEEEECCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHH
MQEMFANPSPVPVKTALNLQSVPVGKVRLPLIELTDEQLQNLQSVLSDFEHSKMI
HHHHHCCCCCCCEEEECCCCCCCCCCEEECCEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376