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The map label for this gene is topA [H]

Identifier: 23099001

GI number: 23099001

Start: 1588748

End: 1590826

Strand: Direct

Name: topA [H]

Synonym: OB1546

Alternate gene names: 23099001

Gene position: 1588748-1590826 (Clockwise)

Preceding gene: 23099000

Following gene: 23099002

Centisome position: 43.76

GC content: 35.74

Gene sequence:

>2079_bases
ATGTCAGATTATTTAGTTATCGTTGAATCACCTGCAAAAGCAAAAACGATTGAACGATATTTAGGGAAAAAATATACCGT
AAAGGCTTCCATGGGTCACATTCGTGATTTACCTAAAAGTCAAACGGGTGTAGACGTTGAAAATAATTTCACTCCGAAAT
ATATAACCATTCGTGGTAAGGGTGATGTTCTTAAAGAATTACGAAAAGCAGCAAAAAAAGCAAAAAAAATCTATCTTGCT
GCCGACCCGGATAGAGAAGGGGAAGCGATTGCTTGGCATCTTGCATATATTTTAGGTGTCGATCCATCTTCAAATTGTCG
TGTTGTTTTTAATGAAATTACCAAAGAGGCAATTAAAGAGTCATTCAAACATCCACGAGCAATTGACCTAGATTTAGTAG
ATGCTCAACAAGCTAGAAGAGTCCTTGATCGATTGGTTGGATATAATATTAGTCCTTTATTATGGAAAAAAGTGAAAAAA
GGATTAAGCGCAGGAAGAGTACAATCTGTTGCTTTGAAGATGATTATAGATCGAGAAAAAGAAATACAAAATTTCAAACC
AGAAGAATATTGGTCTATAGATGCAAAATTCCAAAAGGATAAAGAAATTTTTGACGGATCTTTTTATGGAATAAACGGAA
AAAAAGTAGCATTAAAAAACGAACAAGATGTAAAAGATATCAAGCAACAATTAAACGGGAAGAAATTTGCAGTGGATAAG
GTTAAGAAAAGGGAAAGAAAGAAAAATCCTGCCTTACCCTTCACTACTTCATCTCTTCAACAAGAAGCTGCTAGAAAGTT
AAACTTCCGTGCGAAGAAGACGATGATGATTGCTCAACAGCTTTATGAAGGTATTGACCTAGGGAAAAAATCCGGGGGGA
TTACTGGTTTAATCACATATATGCGTACTGATTCTACTCGTATATCCAATACAGCAAAAGATGAAGCGAAAAACTACATT
GTAGATAATTATGGTGAGAAGTATCTAGGTAAGCAAGCATCAACGAAGAATAAAGAAGGAGCACAAGATGCTCATGAGGC
AATTCGCCCAACTTCTGCGTTAAGAGATCCGAAAAACTTAAAGAATATTTTATCGAGAGATCAATTCCGATTATATAAAT
TGATATGGGAACGGTTTGTAGCAAGTCAAATGGCTCCGGCAGTAATGGATACGATGACCGTTCATTTATTAAATAATAAT
ATCGAATTTCGAGCAACTGGCTCAAAAATTAAGTTTAAAGGATTCATGAAAGTTTATGTGGAAGGTAATGATGATAACAA
GAAAACGGAAGATAAGTTATTACCTGAATTAGAAGAGGGTATGGAAGTAGATTCAAAAGAAATTAAACCGAATCAGCATT
TTACACAGCCACCTCCACGATATACAGACGCACGTCTTGTGCGAACGATGGAAGAAATAGGTATTGGAAGACCGTCTACT
TATGCACCTACACTAGATACGATTCAACGTAGGGGTTATGTAACGATGGATAATAAGCGTTTCATTCCGACTGAGCTTGG
CGAAATTAAATGTGATCTCATGATTGAATTTTTCCCTGAAGTAGTTGACGCAGATTTCACTGCAAAAATGGAAGATGATT
TAGACTCTATTGAAGAAGGGAATATAGAGTGGAAACAAATTATTGATAGCTTTTATAAAGATTTCAAAGTAAGACTGGAT
AAGGCAGAAGACGAGATGGAAAAAGTTGAAATAAAAGATGAACCAGCAGGGATTGATTGTGAGAATTGCGGTCATGAAAT
GGTTTATAAGATGGGGAGATATGGTAAATTCCTCGCTTGTTCAAACTTTCCGGAATGTCGCAATACAAAACCGATCTTAA
AAGAAATTGGTGTTGATTGCCCGAAATGCGAAGAAGGCAATGTGGTTGAACGAAAATCAAAGAAAAAACGAACATTCTAT
GGTTGTGATCGCTTCCCTGAATGTGACTTTGTTTCCTGGGACAAACCCATTGCTAGAAAATGTCCAAAATGTGAATCTAT
GTTAGTAGAGAAAAAGAATAAAAAAGAAACTCAAGTACAGTGTACAAATTGTGAATATAAAGAAGTAGCCCAGACTTAA

Upstream 100 bases:

>100_bases
AAGTTTTAACAGAAGTGTTTGACAAAATAGAGATCTATATTTAGTATTAGAAAAGATTTCATTACGTAAAATTGTCAACC
TCATTTTGGGAGGAAGGTAC

Downstream 100 bases:

>100_bases
TTTTAAGGCTTGTAGAGATGGATTATTCCACTCTATAGGTCTTTTTTATGGCCTGAAAAATTATAAAATTATACAGTAGT
GGATAAACACGTCCAGCGCA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSDYLVIVESPAKAKTIERYLGKKYTVKASMGHIRDLPKSQTGVDVENNFTPKYITIRGKGDVLKELRKAAKKAKKIYLA
ADPDREGEAIAWHLAYILGVDPSSNCRVVFNEITKEAIKESFKHPRAIDLDLVDAQQARRVLDRLVGYNISPLLWKKVKK
GLSAGRVQSVALKMIIDREKEIQNFKPEEYWSIDAKFQKDKEIFDGSFYGINGKKVALKNEQDVKDIKQQLNGKKFAVDK
VKKRERKKNPALPFTTSSLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKKSGGITGLITYMRTDSTRISNTAKDEAKNYI
VDNYGEKYLGKQASTKNKEGAQDAHEAIRPTSALRDPKNLKNILSRDQFRLYKLIWERFVASQMAPAVMDTMTVHLLNNN
IEFRATGSKIKFKGFMKVYVEGNDDNKKTEDKLLPELEEGMEVDSKEIKPNQHFTQPPPRYTDARLVRTMEEIGIGRPST
YAPTLDTIQRRGYVTMDNKRFIPTELGEIKCDLMIEFFPEVVDADFTAKMEDDLDSIEEGNIEWKQIIDSFYKDFKVRLD
KAEDEMEKVEIKDEPAGIDCENCGHEMVYKMGRYGKFLACSNFPECRNTKPILKEIGVDCPKCEEGNVVERKSKKKRTFY
GCDRFPECDFVSWDKPIARKCPKCESMLVEKKNKKETQVQCTNCEYKEVAQT

Sequences:

>Translated_692_residues
MSDYLVIVESPAKAKTIERYLGKKYTVKASMGHIRDLPKSQTGVDVENNFTPKYITIRGKGDVLKELRKAAKKAKKIYLA
ADPDREGEAIAWHLAYILGVDPSSNCRVVFNEITKEAIKESFKHPRAIDLDLVDAQQARRVLDRLVGYNISPLLWKKVKK
GLSAGRVQSVALKMIIDREKEIQNFKPEEYWSIDAKFQKDKEIFDGSFYGINGKKVALKNEQDVKDIKQQLNGKKFAVDK
VKKRERKKNPALPFTTSSLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKKSGGITGLITYMRTDSTRISNTAKDEAKNYI
VDNYGEKYLGKQASTKNKEGAQDAHEAIRPTSALRDPKNLKNILSRDQFRLYKLIWERFVASQMAPAVMDTMTVHLLNNN
IEFRATGSKIKFKGFMKVYVEGNDDNKKTEDKLLPELEEGMEVDSKEIKPNQHFTQPPPRYTDARLVRTMEEIGIGRPST
YAPTLDTIQRRGYVTMDNKRFIPTELGEIKCDLMIEFFPEVVDADFTAKMEDDLDSIEEGNIEWKQIIDSFYKDFKVRLD
KAEDEMEKVEIKDEPAGIDCENCGHEMVYKMGRYGKFLACSNFPECRNTKPILKEIGVDCPKCEEGNVVERKSKKKRTFY
GCDRFPECDFVSWDKPIARKCPKCESMLVEKKNKKETQVQCTNCEYKEVAQT
>Mature_691_residues
SDYLVIVESPAKAKTIERYLGKKYTVKASMGHIRDLPKSQTGVDVENNFTPKYITIRGKGDVLKELRKAAKKAKKIYLAA
DPDREGEAIAWHLAYILGVDPSSNCRVVFNEITKEAIKESFKHPRAIDLDLVDAQQARRVLDRLVGYNISPLLWKKVKKG
LSAGRVQSVALKMIIDREKEIQNFKPEEYWSIDAKFQKDKEIFDGSFYGINGKKVALKNEQDVKDIKQQLNGKKFAVDKV
KKRERKKNPALPFTTSSLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKKSGGITGLITYMRTDSTRISNTAKDEAKNYIV
DNYGEKYLGKQASTKNKEGAQDAHEAIRPTSALRDPKNLKNILSRDQFRLYKLIWERFVASQMAPAVMDTMTVHLLNNNI
EFRATGSKIKFKGFMKVYVEGNDDNKKTEDKLLPELEEGMEVDSKEIKPNQHFTQPPPRYTDARLVRTMEEIGIGRPSTY
APTLDTIQRRGYVTMDNKRFIPTELGEIKCDLMIEFFPEVVDADFTAKMEDDLDSIEEGNIEWKQIIDSFYKDFKVRLDK
AEDEMEKVEIKDEPAGIDCENCGHEMVYKMGRYGKFLACSNFPECRNTKPILKEIGVDCPKCEEGNVVERKSKKKRTFYG
CDRFPECDFVSWDKPIARKCPKCESMLVEKKNKKETQVQCTNCEYKEVAQT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=25.6821829855538, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI4507635, Length=593, Percent_Identity=23.1028667790894, Blast_Score=109, Evalue=9e-24,
Organism=Escherichia coli, GI1787529, Length=755, Percent_Identity=39.7350993377483, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI48994931, Length=107, Percent_Identity=42.0560747663551, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1788061, Length=545, Percent_Identity=22.2018348623853, Blast_Score=103, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17555378, Length=510, Percent_Identity=25.6862745098039, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=564, Percent_Identity=25.531914893617, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=541, Percent_Identity=24.0295748613678, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585251, Length=616, Percent_Identity=23.8636363636364, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24640096, Length=525, Percent_Identity=23.8095238095238, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79389; Mature: 79258

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYLVIVESPAKAKTIERYLGKKYTVKASMGHIRDLPKSQTGVDVENNFTPKYITIRGK
CCCEEEEEECCCHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
GDVLKELRKAAKKAKKIYLAADPDREGEAIAWHLAYILGVDPSSNCRVVFNEITKEAIKE
HHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEEEEECCCCCCCEEHHHHHHHHHHHH
SFKHPRAIDLDLVDAQQARRVLDRLVGYNISPLLWKKVKKGLSAGRVQSVALKMIIDREK
HHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQNFKPEEYWSIDAKFQKDKEIFDGSFYGINGKKVALKNEQDVKDIKQQLNGKKFAVDK
HHHCCCCCHHEECCCCCCCCHHHHCCEEECCCCCEEEECCCHHHHHHHHHHCCCEEHHHH
VKKRERKKNPALPFTTSSLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKKSGGITGLITY
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISNTAKDEAKNYIVDNYGEKYLGKQASTKNKEGAQDAHEAIRPTSALRDPKNL
EECCCCHHCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHHCCHHHH
KNILSRDQFRLYKLIWERFVASQMAPAVMDTMTVHLLNNNIEFRATGSKIKFKGFMKVYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEEEEEE
EGNDDNKKTEDKLLPELEEGMEVDSKEIKPNQHFTQPPPRYTDARLVRTMEEIGIGRPST
ECCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
YAPTLDTIQRRGYVTMDNKRFIPTELGEIKCDLMIEFFPEVVDADFTAKMEDDLDSIEEG
CCCCHHHHHHCCCEEECCCEECCCHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
NIEWKQIIDSFYKDFKVRLDKAEDEMEKVEIKDEPAGIDCENCGHEMVYKMGRYGKFLAC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCHHHHHHHCCCCCEEEE
SNFPECRNTKPILKEIGVDCPKCEEGNVVERKSKKKRTFYGCDRFPECDFVSWDKPIARK
CCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHH
CPKCESMLVEKKNKKETQVQCTNCEYKEVAQT
CCHHHHHHHHHCCCCCCEEEECCCCHHHHHCC
>Mature Secondary Structure 
SDYLVIVESPAKAKTIERYLGKKYTVKASMGHIRDLPKSQTGVDVENNFTPKYITIRGK
CCEEEEEECCCHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCCCCCCCCCCCEEEEECC
GDVLKELRKAAKKAKKIYLAADPDREGEAIAWHLAYILGVDPSSNCRVVFNEITKEAIKE
HHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEEEEEECCCCCCCEEHHHHHHHHHHHH
SFKHPRAIDLDLVDAQQARRVLDRLVGYNISPLLWKKVKKGLSAGRVQSVALKMIIDREK
HHCCCCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EIQNFKPEEYWSIDAKFQKDKEIFDGSFYGINGKKVALKNEQDVKDIKQQLNGKKFAVDK
HHHCCCCCHHEECCCCCCCCHHHHCCEEECCCCCEEEECCCHHHHHHHHHHCCCEEHHHH
VKKRERKKNPALPFTTSSLQQEAARKLNFRAKKTMMIAQQLYEGIDLGKKSGGITGLITY
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
MRTDSTRISNTAKDEAKNYIVDNYGEKYLGKQASTKNKEGAQDAHEAIRPTSALRDPKNL
EECCCCHHCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHCCHHHHCCHHHH
KNILSRDQFRLYKLIWERFVASQMAPAVMDTMTVHLLNNNIEFRATGSKIKFKGFMKVYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCEEEEEEEEEEEE
EGNDDNKKTEDKLLPELEEGMEVDSKEIKPNQHFTQPPPRYTDARLVRTMEEIGIGRPST
ECCCCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
YAPTLDTIQRRGYVTMDNKRFIPTELGEIKCDLMIEFFPEVVDADFTAKMEDDLDSIEEG
CCCCHHHHHHCCCEEECCCEECCCHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
NIEWKQIIDSFYKDFKVRLDKAEDEMEKVEIKDEPAGIDCENCGHEMVYKMGRYGKFLAC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCHHHHHHHCCCCCEEEE
SNFPECRNTKPILKEIGVDCPKCEEGNVVERKSKKKRTFYGCDRFPECDFVSWDKPIARK
CCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHH
CPKCESMLVEKKNKKETQVQCTNCEYKEVAQT
CCHHHHHHHHHCCCCCCEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]