Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 23098961

GI number: 23098961

Start: 1549793

End: 1550734

Strand: Direct

Name: fmt

Synonym: OB1506

Alternate gene names: 23098961

Gene position: 1549793-1550734 (Clockwise)

Preceding gene: 23098960

Following gene: 23098962

Centisome position: 42.69

GC content: 35.03

Gene sequence:

>942_bases
ATGAAACGAATTGTGTTTATGGGTACCCCAGATTTTGCGGTACCAGTATTACAGAAGTTACTAGAATTAAAATATGATAT
TGTGTTAGTAGTTACCCAACCCGATCGTCCAAAAGGCAGAAAAAAAGTTATCACTCCACCACCGGTTAAAGAAGAAGCGA
TTAAACATGATTTAGATATTTTTCAACCAGAGAAACTTCGAGATGATTATAAAAAAATTACAGATTTAAAACCTGACTTA
ATAGTAACAGCGGCATATGGACAAATATTGCCGAAGGAGATATTAGAAATTCCAACCTTTGGCTGTATTAATGTACATGC
TTCACTTCTCCCAGAATTACGTGGCGGTGCACCAATTCATTACGCAATTATGCAAGGGAAAGAAGTGACCGGAGTTACGA
TTATGTATATGGCAGAAAAATTAGATGCAGGAGATATTTTAACGCAAGTTGAAGTTCCAATTGAACAGGATGATCACGTT
GGAACAATGCATGATAAATTATCATTAGCTGGTGCGAATCTGTTAAGTGAAACACTACCATCACTGTTTAATAATGAAAT
AACACCGAAGAAGCAGGATGAAAGTTTGGTTACTTTTGCTTCTAATATTACAAGAGAACAAGAACGGATTGATTGGTCGA
AAACAGCTAATGAAATTATAAATCAAATCAGAGGCTTACACCCATGGCCAGTAGCGTTTACAACCTATGAGGGGAAAGTA
ATGAAAATATGGTGGGGCCAAGAATTATCTAAATCATCGAAATCAGGTAATATTGGTGAGATTATTGAAAAAGATACAGA
TGGCTTTTATGTACAATGTGGTAATAACACGGTTGTAAAAGTTAGTGAAATTCAACCTGCTGGTAAAAAACGCATGTTGG
TTGAAGATTATTTGAAAGGTTCTCAAGATCGAATAAAAATAGGAATTAAATTAGGTGAATAA

Upstream 100 bases:

>100_bases
TTTTTCGGATGAAATTAGAAAGCAACAAATGCAATTAACTATCGATATGCAACCGTATTATATGATGTAAATAAGTGTTC
AATGAAAGAAGGTATAAATA

Downstream 100 bases:

>100_bases
GATGAAGTATCAACTACGTAATACAATGCTCGATATATTAATTCGAATTGAAAAGGATCATGGTTTCAGTAATTTACTTC
TAAACCATGAATTAAAACAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKRIVFMGTPDFAVPVLQKLLELKYDIVLVVTQPDRPKGRKKVITPPPVKEEAIKHDLDIFQPEKLRDDYKKITDLKPDL
IVTAAYGQILPKEILEIPTFGCINVHASLLPELRGGAPIHYAIMQGKEVTGVTIMYMAEKLDAGDILTQVEVPIEQDDHV
GTMHDKLSLAGANLLSETLPSLFNNEITPKKQDESLVTFASNITREQERIDWSKTANEIINQIRGLHPWPVAFTTYEGKV
MKIWWGQELSKSSKSGNIGEIIEKDTDGFYVQCGNNTVVKVSEIQPAGKKRMLVEDYLKGSQDRIKIGIKLGE

Sequences:

>Translated_313_residues
MKRIVFMGTPDFAVPVLQKLLELKYDIVLVVTQPDRPKGRKKVITPPPVKEEAIKHDLDIFQPEKLRDDYKKITDLKPDL
IVTAAYGQILPKEILEIPTFGCINVHASLLPELRGGAPIHYAIMQGKEVTGVTIMYMAEKLDAGDILTQVEVPIEQDDHV
GTMHDKLSLAGANLLSETLPSLFNNEITPKKQDESLVTFASNITREQERIDWSKTANEIINQIRGLHPWPVAFTTYEGKV
MKIWWGQELSKSSKSGNIGEIIEKDTDGFYVQCGNNTVVKVSEIQPAGKKRMLVEDYLKGSQDRIKIGIKLGE
>Mature_313_residues
MKRIVFMGTPDFAVPVLQKLLELKYDIVLVVTQPDRPKGRKKVITPPPVKEEAIKHDLDIFQPEKLRDDYKKITDLKPDL
IVTAAYGQILPKEILEIPTFGCINVHASLLPELRGGAPIHYAIMQGKEVTGVTIMYMAEKLDAGDILTQVEVPIEQDDHV
GTMHDKLSLAGANLLSETLPSLFNNEITPKKQDESLVTFASNITREQERIDWSKTANEIINQIRGLHPWPVAFTTYEGKV
MKIWWGQELSKSSKSGNIGEIIEKDTDGFYVQCGNNTVVKVSEIQPAGKKRMLVEDYLKGSQDRIKIGIKLGE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=232, Percent_Identity=31.4655172413793, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI21614513, Length=240, Percent_Identity=27.9166666666667, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI238814322, Length=259, Percent_Identity=25.4826254826255, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789683, Length=311, Percent_Identity=41.4790996784566, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788589, Length=258, Percent_Identity=30.6201550387597, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133930964, Length=268, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319458, Length=357, Percent_Identity=26.3305322128852, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45550868, Length=303, Percent_Identity=32.013201320132, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28571984, Length=227, Percent_Identity=33.920704845815, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585660, Length=254, Percent_Identity=27.5590551181102, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_OCEIH (Q8ER25)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692427.1
- ProteinModelPortal:   Q8ER25
- SMR:   Q8ER25
- GeneID:   1017768
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1506
- NMPDR:   fig|221109.1.peg.1508
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- BioCyc:   OIHE221109:OB1506-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35130; Mature: 35130

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIVFMGTPDFAVPVLQKLLELKYDIVLVVTQPDRPKGRKKVITPPPVKEEAIKHDLDI
CCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCC
FQPEKLRDDYKKITDLKPDLIVTAAYGQILPKEILEIPTFGCINVHASLLPELRGGAPIH
CCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEE
YAIMQGKEVTGVTIMYMAEKLDAGDILTQVEVPIEQDDHVGTMHDKLSLAGANLLSETLP
EEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SLFNNEITPKKQDESLVTFASNITREQERIDWSKTANEIINQIRGLHPWPVAFTTYEGKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCEE
MKIWWGQELSKSSKSGNIGEIIEKDTDGFYVQCGNNTVVKVSEIQPAGKKRMLVEDYLKG
EEEECCCHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCC
SQDRIKIGIKLGE
CCCEEEEEEEECC
>Mature Secondary Structure
MKRIVFMGTPDFAVPVLQKLLELKYDIVLVVTQPDRPKGRKKVITPPPVKEEAIKHDLDI
CCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCCC
FQPEKLRDDYKKITDLKPDLIVTAAYGQILPKEILEIPTFGCINVHASLLPELRGGAPIH
CCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEE
YAIMQGKEVTGVTIMYMAEKLDAGDILTQVEVPIEQDDHVGTMHDKLSLAGANLLSETLP
EEEECCCCCCCEEEEEEEHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SLFNNEITPKKQDESLVTFASNITREQERIDWSKTANEIINQIRGLHPWPVAFTTYEGKV
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCEE
MKIWWGQELSKSSKSGNIGEIIEKDTDGFYVQCGNNTVVKVSEIQPAGKKRMLVEDYLKG
EEEECCCHHHCCCCCCCHHHHHHCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHCC
SQDRIKIGIKLGE
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376