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The map label for this gene is priA [H]

Identifier: 23098960

GI number: 23098960

Start: 1547354

End: 1549762

Strand: Direct

Name: priA [H]

Synonym: OB1505

Alternate gene names: 23098960

Gene position: 1547354-1549762 (Clockwise)

Preceding gene: 23098959

Following gene: 23098961

Centisome position: 42.62

GC content: 36.94

Gene sequence:

>2409_bases
TTGAATATAGCTAGTGTTATTGTGGATGTGCCATCTAGTTCAACAAATCAAACATTCGATTATTGTATTCCTGAACAGTT
TCAGGGGGTTATCCAAAAGGGGATGAGAGTAATTGTACCCTTTGGGCCAAGAAAAATTATGGGATTTGTGACAGGTGTTA
AGGATGGAACTTCTTTATCAAAGGTAAAAGATATCATTGATGTTTTGGATATAACCCCTGTTCTAACAGATGAGTTATTA
AGTTTAAGTAGGTGGGTTTCTGAGAAAACATTAAGTCTTTCTATTACTGTTCTGCAAACGATGCTTCCACAGGTTTTAAA
ATCACAGTATAGCAAAGACTTAGTGAGAGAGACAGAAGCCGAATTACCAGATGAGCTTGAGGCATTATTTGCTAGTCGTG
AGTCGATCGATTTTGATGAGGTTGGAAAATCAACCATCTCTTATAGAATAATTAAAAATCATATTGATTCTGGAGATTTA
TCAGTGTCATATCATGTGCAATCTAGAATCACGAAGAAATATCAAACGATGGTAGTTCCTTCAAAATCCCCAATTCAGTT
AGAAGAGGAGATAATGAGTTTACCGAAGAATGCGAGTAAACAAAAGCAATTATTAACGTATCTTGCAGATAATCAGAGTG
AAATAGATAAGAAACAACTATTACAAGAAACCAATACTTCTGTACAAACAGTAAAGGCAATTGAAGAAAAGGGAATAATC
AAAACCTATAAGAAAGAAGTCTTTCGTGATCCATACAAAGATCAATATATCGAACCTACTTTCCCACTAAATCTAACGAA
AGAACAAGCCCAAGCTATGGAACCAATAAAATATAGTCTGGATAACCACATCAATGAAACTTTCTTATTACATGGGGTAA
CTGGTAGTGGGAAAACTGAGATTTACTTACAAGCGATTCAACAAGTAATCGAGGAAGGTAAAGAGGCTATTGTGCTTGTA
CCAGAAATTTCTCTAACACCTCAAATGGTAAATAGATTTAAAGGAAGATTCGGTAGTAAAGTAGCAGTAATGCATAGTGG
ATTATCTTCCGGAGAAAAGTACGATGAATGGAGGAAAATTCATCGACAAGAAGTACAGGTAGTTGTAGGTGCTCGTTCAG
CAATATTTGCTCCTTTTGAAAATTTAGGAATAATTATTATAGACGAAGAGCATGAAACGACTTATAAACAAGAGGATCAA
CCCCGTTACCATGCAAGAGATGTCGCGATAGAAAGAGCAGCGTATCATCAATGTCCGGTTGTGCTTGGGAGTGCCACTCC
ATCGCTTGAATCCTTTGCTAGAGCCTCCAAAAATGTATATACCCTATTAACATTACAGGAGAGAACCAATCATCAAACCA
TGCCAAAGGTTGAGATTGTAGATATGCGTAATGAATTACATGCTGGTAATCGATCGATGTTTTCTCGACCATTAATGGAA
CAGATGGATGAAAGATTACGTCGCGGAGAACAAATTGTACTTTTATTAAATCGTCGTGGATATTCTACATTTGTCATGTG
TAGAGATTGTGGACACGTATGCGAATGTCCCCATTGTGATATTGCACTGACTTATCATAAAAATACACAGCGTTTGAAAT
GCCATTATTGTTCTTATGAACAATCGGTGCCACAGACTTGCCCTGAGTGTGAAAGTGATTTAATTCGATATTTCGGTACA
GGTACACAAAAAGTAGAAGAAGCTTTAGCAAAACAAATACCTGAGGCTAGAGTAATTAGGATGGATGTCGATACAACAAG
AAGAAAAGGTTCCCATGAAAAACTCCTGAGACAGTTTTCCGAGAAAAAGGCAGATATTTTATTAGGGACACAAATGATTG
CTAAGGGTCTTGATTTTGCTAATGTTACATTAGTGGGGGTACTTTCTGCAGACTCGATGTTACACATCCCAGACTTTCGA
TCATCGGAACGAACCTTCCAATTACTAACGCAGGTTAGTGGAAGAGCTGGGAGACATGAGCTTCCAGGGGAAGTAATTGT
TCAGACGTATACACCAGAACATTATAGTATTGAGTTTGCATCTGTGTATGATTACGACAACTTCTATCGGAAAGAAATGG
CGATGAGAAAGGCATTTCAATATCCACCTTATGTGTTTTTAGGATTAATAACAGTAGCTCATGAAAATCATGCGAGAGCA
ATTCAAGTTACCCAGGAAATTGTACAGATGCTTTCCAATATTGTGGAAGAAAGGACTACAGTATTAGGTCCAACCCCATC
TCCAGTGCCTAGGATCAAAGATAGATATCGCTATCAATGCATGATAAAATACAAAAATGAGCCGAATTTGCATGAATATC
TAGGACATATACAGGATCATTTTTCGGATGAAATTAGAAAGCAACAAATGCAATTAACTATCGATATGCAACCGTATTAT
ATGATGTAA

Upstream 100 bases:

>100_bases
ATAAGAAAAAACAGCAAAAATCAATTCATCAAGCAACAAAATCAGATATAGCTTATCAATTATTTGAATTAATTTCCCAA
GACTTAAAGGATGATGACCG

Downstream 100 bases:

>100_bases
ATAAGTGTTCAATGAAAGAAGGTATAAATAATGAAACGAATTGTGTTTATGGGTACCCCAGATTTTGCGGTACCAGTATT
ACAGAAGTTACTAGAATTAA

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 802; Mature: 802

Protein sequence:

>802_residues
MNIASVIVDVPSSSTNQTFDYCIPEQFQGVIQKGMRVIVPFGPRKIMGFVTGVKDGTSLSKVKDIIDVLDITPVLTDELL
SLSRWVSEKTLSLSITVLQTMLPQVLKSQYSKDLVRETEAELPDELEALFASRESIDFDEVGKSTISYRIIKNHIDSGDL
SVSYHVQSRITKKYQTMVVPSKSPIQLEEEIMSLPKNASKQKQLLTYLADNQSEIDKKQLLQETNTSVQTVKAIEEKGII
KTYKKEVFRDPYKDQYIEPTFPLNLTKEQAQAMEPIKYSLDNHINETFLLHGVTGSGKTEIYLQAIQQVIEEGKEAIVLV
PEISLTPQMVNRFKGRFGSKVAVMHSGLSSGEKYDEWRKIHRQEVQVVVGARSAIFAPFENLGIIIIDEEHETTYKQEDQ
PRYHARDVAIERAAYHQCPVVLGSATPSLESFARASKNVYTLLTLQERTNHQTMPKVEIVDMRNELHAGNRSMFSRPLME
QMDERLRRGEQIVLLLNRRGYSTFVMCRDCGHVCECPHCDIALTYHKNTQRLKCHYCSYEQSVPQTCPECESDLIRYFGT
GTQKVEEALAKQIPEARVIRMDVDTTRRKGSHEKLLRQFSEKKADILLGTQMIAKGLDFANVTLVGVLSADSMLHIPDFR
SSERTFQLLTQVSGRAGRHELPGEVIVQTYTPEHYSIEFASVYDYDNFYRKEMAMRKAFQYPPYVFLGLITVAHENHARA
IQVTQEIVQMLSNIVEERTTVLGPTPSPVPRIKDRYRYQCMIKYKNEPNLHEYLGHIQDHFSDEIRKQQMQLTIDMQPYY
MM

Sequences:

>Translated_802_residues
MNIASVIVDVPSSSTNQTFDYCIPEQFQGVIQKGMRVIVPFGPRKIMGFVTGVKDGTSLSKVKDIIDVLDITPVLTDELL
SLSRWVSEKTLSLSITVLQTMLPQVLKSQYSKDLVRETEAELPDELEALFASRESIDFDEVGKSTISYRIIKNHIDSGDL
SVSYHVQSRITKKYQTMVVPSKSPIQLEEEIMSLPKNASKQKQLLTYLADNQSEIDKKQLLQETNTSVQTVKAIEEKGII
KTYKKEVFRDPYKDQYIEPTFPLNLTKEQAQAMEPIKYSLDNHINETFLLHGVTGSGKTEIYLQAIQQVIEEGKEAIVLV
PEISLTPQMVNRFKGRFGSKVAVMHSGLSSGEKYDEWRKIHRQEVQVVVGARSAIFAPFENLGIIIIDEEHETTYKQEDQ
PRYHARDVAIERAAYHQCPVVLGSATPSLESFARASKNVYTLLTLQERTNHQTMPKVEIVDMRNELHAGNRSMFSRPLME
QMDERLRRGEQIVLLLNRRGYSTFVMCRDCGHVCECPHCDIALTYHKNTQRLKCHYCSYEQSVPQTCPECESDLIRYFGT
GTQKVEEALAKQIPEARVIRMDVDTTRRKGSHEKLLRQFSEKKADILLGTQMIAKGLDFANVTLVGVLSADSMLHIPDFR
SSERTFQLLTQVSGRAGRHELPGEVIVQTYTPEHYSIEFASVYDYDNFYRKEMAMRKAFQYPPYVFLGLITVAHENHARA
IQVTQEIVQMLSNIVEERTTVLGPTPSPVPRIKDRYRYQCMIKYKNEPNLHEYLGHIQDHFSDEIRKQQMQLTIDMQPYY
MM
>Mature_802_residues
MNIASVIVDVPSSSTNQTFDYCIPEQFQGVIQKGMRVIVPFGPRKIMGFVTGVKDGTSLSKVKDIIDVLDITPVLTDELL
SLSRWVSEKTLSLSITVLQTMLPQVLKSQYSKDLVRETEAELPDELEALFASRESIDFDEVGKSTISYRIIKNHIDSGDL
SVSYHVQSRITKKYQTMVVPSKSPIQLEEEIMSLPKNASKQKQLLTYLADNQSEIDKKQLLQETNTSVQTVKAIEEKGII
KTYKKEVFRDPYKDQYIEPTFPLNLTKEQAQAMEPIKYSLDNHINETFLLHGVTGSGKTEIYLQAIQQVIEEGKEAIVLV
PEISLTPQMVNRFKGRFGSKVAVMHSGLSSGEKYDEWRKIHRQEVQVVVGARSAIFAPFENLGIIIIDEEHETTYKQEDQ
PRYHARDVAIERAAYHQCPVVLGSATPSLESFARASKNVYTLLTLQERTNHQTMPKVEIVDMRNELHAGNRSMFSRPLME
QMDERLRRGEQIVLLLNRRGYSTFVMCRDCGHVCECPHCDIALTYHKNTQRLKCHYCSYEQSVPQTCPECESDLIRYFGT
GTQKVEEALAKQIPEARVIRMDVDTTRRKGSHEKLLRQFSEKKADILLGTQMIAKGLDFANVTLVGVLSADSMLHIPDFR
SSERTFQLLTQVSGRAGRHELPGEVIVQTYTPEHYSIEFASVYDYDNFYRKEMAMRKAFQYPPYVFLGLITVAHENHARA
IQVTQEIVQMLSNIVEERTTVLGPTPSPVPRIKDRYRYQCMIKYKNEPNLHEYLGHIQDHFSDEIRKQQMQLTIDMQPYY
MM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=578, Percent_Identity=38.2352941176471, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91902; Mature: 91902

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIASVIVDVPSSSTNQTFDYCIPEQFQGVIQKGMRVIVPFGPRKIMGFVTGVKDGTSLS
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCHHH
KVKDIIDVLDITPVLTDELLSLSRWVSEKTLSLSITVLQTMLPQVLKSQYSKDLVRETEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELPDELEALFASRESIDFDEVGKSTISYRIIKNHIDSGDLSVSYHVQSRITKKYQTMVVP
CCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHEEECC
SKSPIQLEEEIMSLPKNASKQKQLLTYLADNQSEIDKKQLLQETNTSVQTVKAIEEKGII
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
KTYKKEVFRDPYKDQYIEPTFPLNLTKEQAQAMEPIKYSLDNHINETFLLHGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCCEEEEEECCCCCCHH
IYLQAIQQVIEEGKEAIVLVPEISLTPQMVNRFKGRFGSKVAVMHSGLSSGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
HRQEVQVVVGARSAIFAPFENLGIIIIDEEHETTYKQEDQPRYHARDVAIERAAYHQCPV
HHHHHHHHEECCHHHCCCHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
VLGSATPSLESFARASKNVYTLLTLQERTNHQTMPKVEIVDMRNELHAGNRSMFSRPLME
EECCCCCCHHHHHHCCCCEEEEEEEEHHCCCCCCCCEEEEECHHHHCCCCHHHHHHHHHH
QMDERLRRGEQIVLLLNRRGYSTFVMCRDCGHVCECPHCDIALTYHKNTQRLKCHYCSYE
HHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCH
QSVPQTCPECESDLIRYFGTGTQKVEEALAKQIPEARVIRMDVDTTRRKGSHEKLLRQFS
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHH
EKKADILLGTQMIAKGLDFANVTLVGVLSADSMLHIPDFRSSERTFQLLTQVSGRAGRHE
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCC
LPGEVIVQTYTPEHYSIEFASVYDYDNFYRKEMAMRKAFQYPPYVFLGLITVAHENHARA
CCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHH
IQVTQEIVQMLSNIVEERTTVLGPTPSPVPRIKDRYRYQCMIKYKNEPNLHEYLGHIQDH
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCEEEEEEEECCCCCHHHHHHHHHHH
FSDEIRKQQMQLTIDMQPYYMM
HHHHHHHHHHEEEEECCCCCCC
>Mature Secondary Structure
MNIASVIVDVPSSSTNQTFDYCIPEQFQGVIQKGMRVIVPFGPRKIMGFVTGVKDGTSLS
CCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCCCHHH
KVKDIIDVLDITPVLTDELLSLSRWVSEKTLSLSITVLQTMLPQVLKSQYSKDLVRETEA
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELPDELEALFASRESIDFDEVGKSTISYRIIKNHIDSGDLSVSYHVQSRITKKYQTMVVP
CCHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHEEECC
SKSPIQLEEEIMSLPKNASKQKQLLTYLADNQSEIDKKQLLQETNTSVQTVKAIEEKGII
CCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
KTYKKEVFRDPYKDQYIEPTFPLNLTKEQAQAMEPIKYSLDNHINETFLLHGVTGSGKTE
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCHHHHHHHCCCCCEEEEEECCCCCCHH
IYLQAIQQVIEEGKEAIVLVPEISLTPQMVNRFKGRFGSKVAVMHSGLSSGEKYDEWRKI
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
HRQEVQVVVGARSAIFAPFENLGIIIIDEEHETTYKQEDQPRYHARDVAIERAAYHQCPV
HHHHHHHHEECCHHHCCCHHHCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCE
VLGSATPSLESFARASKNVYTLLTLQERTNHQTMPKVEIVDMRNELHAGNRSMFSRPLME
EECCCCCCHHHHHHCCCCEEEEEEEEHHCCCCCCCCEEEEECHHHHCCCCHHHHHHHHHH
QMDERLRRGEQIVLLLNRRGYSTFVMCRDCGHVCECPHCDIALTYHKNTQRLKCHYCSYE
HHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECCCH
QSVPQTCPECESDLIRYFGTGTQKVEEALAKQIPEARVIRMDVDTTRRKGSHEKLLRQFS
HCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHHHHHH
EKKADILLGTQMIAKGLDFANVTLVGVLSADSMLHIPDFRSSERTFQLLTQVSGRAGRHE
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCC
LPGEVIVQTYTPEHYSIEFASVYDYDNFYRKEMAMRKAFQYPPYVFLGLITVAHENHARA
CCCCEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHH
IQVTQEIVQMLSNIVEERTTVLGPTPSPVPRIKDRYRYQCMIKYKNEPNLHEYLGHIQDH
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCEEEEEEEECCCCCHHHHHHHHHHH
FSDEIRKQQMQLTIDMQPYYMM
HHHHHHHHHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]