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The map label for this gene is pyrF

Identifier: 23098949

GI number: 23098949

Start: 1536002

End: 1536697

Strand: Direct

Name: pyrF

Synonym: OB1494

Alternate gene names: 23098949

Gene position: 1536002-1536697 (Clockwise)

Preceding gene: 23098948

Following gene: 23098950

Centisome position: 42.31

GC content: 37.36

Gene sequence:

>696_bases
ATGACCATGTTTGTAGCACTAGATTTTCCTGATTGGAAACAAACAGAGGCATTTCTATATAAAAATGAATTGCAAGGTGT
TCCTGTTAAAGTTGGTATGGAACTGTTTTATAAAGAGGGCCCTAGCGTAATTGAGAAATTAAAAAGGAATAATCACTCTA
TTTTTTTAGATCTTAAATTACATGACATACCAAATACAGTCCATCGAGCAATGAAAAATATTGCTTCCTTAGGGGTCGAT
TTAGTTACAATACATACACTTGGCGGAAGTGAAATGATTGCTCAGGCGAAGCGTGGACTTGAACATTCACAAACAAAACT
GATTGCAGTAACCCTGTTAACATCGGTAGATGAAGAAGTAGTTCAACATCAATTACGTTTACCTGGAAATATTCCAGCAA
ATGTTGCTCATTTAGCGAGCTTAGCAAAAGCTAATGGAGCGGATGGAGTCGTTAGCTCTGTACATGAAGTAGCTTCTATC
AAAAAAACTTGTGATACATCTTTCTTAACGTTAACCCCAGGAATACGATTTGATAGTTCGGATGTTCATGATCAAAAACG
TGTTGCGACACCAGAAATGGCAAATCAAGTTGGAAGTGACTTTATCGTAGTAGGTAGAGCGATTACGCAATCTGATCATC
CTTATACAGCATATATGGAAGCAAAGAAACGATGGGAGGAAGCTTCGGATGAATAA

Upstream 100 bases:

>100_bases
CATTTGTTTGTGTAGATATAATAAATGAGTTACCAGAAGTATTGGAGCAGTATGGATTTAATTCAATAGAAGATGTTATC
GAAAAAAGGGGGATTACGGT

Downstream 100 bases:

>100_bases
ACAAATTACAAAGGAACTACTAGAAATTGGAGCAGTACAGATTAATTTAGATACATATTTTACTTGGACATCAGGATTAA
AATCTCCCATCTATTGTGAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTMFVALDFPDWKQTEAFLYKNELQGVPVKVGMELFYKEGPSVIEKLKRNNHSIFLDLKLHDIPNTVHRAMKNIASLGVD
LVTIHTLGGSEMIAQAKRGLEHSQTKLIAVTLLTSVDEEVVQHQLRLPGNIPANVAHLASLAKANGADGVVSSVHEVASI
KKTCDTSFLTLTPGIRFDSSDVHDQKRVATPEMANQVGSDFIVVGRAITQSDHPYTAYMEAKKRWEEASDE

Sequences:

>Translated_231_residues
MTMFVALDFPDWKQTEAFLYKNELQGVPVKVGMELFYKEGPSVIEKLKRNNHSIFLDLKLHDIPNTVHRAMKNIASLGVD
LVTIHTLGGSEMIAQAKRGLEHSQTKLIAVTLLTSVDEEVVQHQLRLPGNIPANVAHLASLAKANGADGVVSSVHEVASI
KKTCDTSFLTLTPGIRFDSSDVHDQKRVATPEMANQVGSDFIVVGRAITQSDHPYTAYMEAKKRWEEASDE
>Mature_230_residues
TMFVALDFPDWKQTEAFLYKNELQGVPVKVGMELFYKEGPSVIEKLKRNNHSIFLDLKLHDIPNTVHRAMKNIASLGVDL
VTIHTLGGSEMIAQAKRGLEHSQTKLIAVTLLTSVDEEVVQHQLRLPGNIPANVAHLASLAKANGADGVVSSVHEVASIK
KTCDTSFLTLTPGIRFDSSDVHDQKRVATPEMANQVGSDFIVVGRAITQSDHPYTAYMEAKKRWEEASDE

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=215, Percent_Identity=40, Blast_Score=138, Evalue=4e-34,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_OCEIH (Q8ER36)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692415.1
- ProteinModelPortal:   Q8ER36
- GeneID:   1017752
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1494
- NMPDR:   fig|221109.1.peg.1496
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- BioCyc:   OIHE221109:OB1494-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25504; Mature: 25373

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 59-59 BINDING 8-8 BINDING 30-30 BINDING 114-114 BINDING 176-176 BINDING 185-185 BINDING 205-205 BINDING 206-206

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFVALDFPDWKQTEAFLYKNELQGVPVKVGMELFYKEGPSVIEKLKRNNHSIFLDLKL
CEEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEE
HDIPNTVHRAMKNIASLGVDLVTIHTLGGSEMIAQAKRGLEHSQTKLIAVTLLTSVDEEV
CCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VQHQLRLPGNIPANVAHLASLAKANGADGVVSSVHEVASIKKTCDTSFLTLTPGIRFDSS
HHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DVHDQKRVATPEMANQVGSDFIVVGRAITQSDHPYTAYMEAKKRWEEASDE
CCCCHHHCCCHHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMFVALDFPDWKQTEAFLYKNELQGVPVKVGMELFYKEGPSVIEKLKRNNHSIFLDLKL
EEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEE
HDIPNTVHRAMKNIASLGVDLVTIHTLGGSEMIAQAKRGLEHSQTKLIAVTLLTSVDEEV
CCCCHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VQHQLRLPGNIPANVAHLASLAKANGADGVVSSVHEVASIKKTCDTSFLTLTPGIRFDSS
HHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DVHDQKRVATPEMANQVGSDFIVVGRAITQSDHPYTAYMEAKKRWEEASDE
CCCCHHHCCCHHHHHHCCCCEEEEEHHHHCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376