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The map label for this gene is pyrB

Identifier: 23098943

GI number: 23098943

Start: 1527878

End: 1528807

Strand: Direct

Name: pyrB

Synonym: OB1488

Alternate gene names: 23098943

Gene position: 1527878-1528807 (Clockwise)

Preceding gene: 23098942

Following gene: 23098944

Centisome position: 42.08

GC content: 36.34

Gene sequence:

>930_bases
ATGAGACATTTTATTTCTGTTAATCAATTGGAAGCCGATGAAATGTATCAAATAATACGAAAAGCAAATGAATTAAGAGA
CCGACCAAATCAATTAAATCGCCAATTATTTGCTGGTAATCTGTTTTTTGAACCGAGTACTCGAACAAAAATGAGTTTTT
CAGTTGCGGAACGAAAGTTAGGTGTGGAGATATTGGATTTCCATACAGAAGCATCGAGTTTGGCAAAAGGTGAATCGTTA
TATGATACAGCAAAAACATTTGAAGCGATTGGGGCTAATTTCTTAGTAATAAGACATCCTTCAGATCAATGGATAAGTGA
ATTGGAGCAGGGAGGAAAATTAAACATACCAGTCATTAATGCTGGTTCTGGCAAAGAAGAACATCCAACTCAATGTTTGC
TTGATCTACTAACGATGTATCAAGAGTTTGGTTCAATTAAAGGGTTGAAGGTTGTGATTGCTGGAGATATAAAACATAGT
CGAGTAGCTAAATCCAATGCAATGGCGCTTACGAAACTAGGTGCGAAAGTAATTTTTAGTGCCGCACCAGGATTTGAGGA
CCATACGTTAGATTATCCATACCTGACCATGGATGAAGCCATAGAAGAAGCTGACGTGTTAATGTTACTGAGAATTCAAC
ATGAGCGACATCTGCATAAAGCAGAGACTTCAGATTACCTATCACTCTATGGTTTAACGAAAGAAAGATATAAAAAGTTA
CAAGATCATGCAATTCTTATGCATCCAGCTCCGATAAATCGTGGGGTAGAAATAGATACAAATTTAGTAGAGAGTGAAAA
ATCAAGGATTTTTAAACAAATGTCCAATGGGGTTTACGTACGAATGGCAATTATCATGCACGTATTATCAGAATGGGGGA
TTATTCATGAAAACAATCTTATTAAACGCAAATCGCTTACTGCCATCTAA

Upstream 100 bases:

>100_bases
TATATATTCCAGGATACGTATAGCTCTCTGCAACCATTTTGCAGAGAGCTTTTTTTATAACTAAATCTTCGTTATTACGG
ATACATGAAGGAGGAAGTCG

Downstream 100 bases:

>100_bases
TGAATTAGAGAAAGTAGAAGTACTTATAGAAGATAATAAAATAATAAAAATTGCATCTCAGATTCCTGATAAGGTAGAAA
ACTATATTGATATAGAAGGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRHFISVNQLEADEMYQIIRKANELRDRPNQLNRQLFAGNLFFEPSTRTKMSFSVAERKLGVEILDFHTEASSLAKGESL
YDTAKTFEAIGANFLVIRHPSDQWISELEQGGKLNIPVINAGSGKEEHPTQCLLDLLTMYQEFGSIKGLKVVIAGDIKHS
RVAKSNAMALTKLGAKVIFSAAPGFEDHTLDYPYLTMDEAIEEADVLMLLRIQHERHLHKAETSDYLSLYGLTKERYKKL
QDHAILMHPAPINRGVEIDTNLVESEKSRIFKQMSNGVYVRMAIIMHVLSEWGIIHENNLIKRKSLTAI

Sequences:

>Translated_309_residues
MRHFISVNQLEADEMYQIIRKANELRDRPNQLNRQLFAGNLFFEPSTRTKMSFSVAERKLGVEILDFHTEASSLAKGESL
YDTAKTFEAIGANFLVIRHPSDQWISELEQGGKLNIPVINAGSGKEEHPTQCLLDLLTMYQEFGSIKGLKVVIAGDIKHS
RVAKSNAMALTKLGAKVIFSAAPGFEDHTLDYPYLTMDEAIEEADVLMLLRIQHERHLHKAETSDYLSLYGLTKERYKKL
QDHAILMHPAPINRGVEIDTNLVESEKSRIFKQMSNGVYVRMAIIMHVLSEWGIIHENNLIKRKSLTAI
>Mature_309_residues
MRHFISVNQLEADEMYQIIRKANELRDRPNQLNRQLFAGNLFFEPSTRTKMSFSVAERKLGVEILDFHTEASSLAKGESL
YDTAKTFEAIGANFLVIRHPSDQWISELEQGGKLNIPVINAGSGKEEHPTQCLLDLLTMYQEFGSIKGLKVVIAGDIKHS
RVAKSNAMALTKLGAKVIFSAAPGFEDHTLDYPYLTMDEAIEEADVLMLLRIQHERHLHKAETSDYLSLYGLTKERYKKL
QDHAILMHPAPINRGVEIDTNLVESEKSRIFKQMSNGVYVRMAIIMHVLSEWGIIHENNLIKRKSLTAI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=32.7974276527331, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=30.5921052631579, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=31.1526479750779, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=30.2931596091205, Blast_Score=129, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=30.8441558441558, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_OCEIH (Q8ER38)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692409.1
- ProteinModelPortal:   Q8ER38
- SMR:   Q8ER38
- GeneID:   1017744
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1488
- NMPDR:   fig|221109.1.peg.1490
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   OIHE221109:OB1488-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35078; Mature: 35078

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFISVNQLEADEMYQIIRKANELRDRPNQLNRQLFAGNLFFEPSTRTKMSFSVAERKL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEECEEEECCCCCCCHHHHHHHHHH
GVEILDFHTEASSLAKGESLYDTAKTFEAIGANFLVIRHPSDQWISELEQGGKLNIPVIN
CCEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEE
AGSGKEEHPTQCLLDLLTMYQEFGSIKGLKVVIAGDIKHSRVAKSNAMALTKLGAKVIFS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCHHHEEE
AAPGFEDHTLDYPYLTMDEAIEEADVLMLLRIQHERHLHKAETSDYLSLYGLTKERYKKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
QDHAILMHPAPINRGVEIDTNLVESEKSRIFKQMSNGVYVRMAIIMHVLSEWGIIHENNL
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCEEECCCC
IKRKSLTAI
CCCCCCCCC
>Mature Secondary Structure
MRHFISVNQLEADEMYQIIRKANELRDRPNQLNRQLFAGNLFFEPSTRTKMSFSVAERKL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEECEEEECCCCCCCHHHHHHHHHH
GVEILDFHTEASSLAKGESLYDTAKTFEAIGANFLVIRHPSDQWISELEQGGKLNIPVIN
CCEEEEECCCHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCCEEEEE
AGSGKEEHPTQCLLDLLTMYQEFGSIKGLKVVIAGDIKHSRVAKSNAMALTKLGAKVIFS
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCHHHHHHCHHHEEE
AAPGFEDHTLDYPYLTMDEAIEEADVLMLLRIQHERHLHKAETSDYLSLYGLTKERYKKL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHH
QDHAILMHPAPINRGVEIDTNLVESEKSRIFKQMSNGVYVRMAIIMHVLSEWGIIHENNL
HCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHCCEEECCCC
IKRKSLTAI
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376