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The map label for this gene is amtB [C]

Identifier: 23098666

GI number: 23098666

Start: 1260475

End: 1261845

Strand: Reverse

Name: amtB [C]

Synonym: OB1211

Alternate gene names: 23098666

Gene position: 1261845-1260475 (Counterclockwise)

Preceding gene: 23098667

Following gene: 23098663

Centisome position: 34.76

GC content: 37.49

Gene sequence:

>1371_bases
ATGTTGAAGAAAAGTTTTCTTATATTTACAGCACTGTTACTTTTAGCTACTCCAGTTTACGCAGCGACACCTACACCTGA
ATCGTTAGAGCTTTCTGTTAACCTAATTTGGGTAATGCTTGGTGCGTTTTTAGTATTTTTTATGCATGCAGGATTTGCTA
TGGTAGAGTCTGGCTTTACTAGATCCAAAAATACCTTAAATATCTTGATGAAGAATTTCTTAACAATAAGTGTTGCATCT
ATATTATTTTTCTTAGTAGGTTATGGTATTATGTTCGGTTCTTCTGCCGGATCCATTATAGGTAGTGACGGATTTTTACT
AAGCGAAATTAGCGAAATAGACTTTTTCGTATTTCAAGCTATGTTTGTTGCCACTTGTGCAACAATTATATCTGGAGCTG
TAGCAGAACGAATTAAATTAGGTAGTTATATCCTTATTGTTGCGGCAATGACCACAATCATATATCCAGTTGTTGGACAT
TGGGTGTGGCAAGGTGATGGATGGTTAACCTCACTAGGATTTAGTGACTTCGCAGGGTCTACTGTAGTTCACCTAACAGG
TGCTGTGGGAGCTTTCGTAGTAGTACTAATACTCGGTCCTCGTATCGGAAAATACAAAGGGAAGAAAGTTAATGTTATCC
AAGGCCATAATTTACCTTTAGGAGCTCTAGGTGTTTTTATCCTTTGGTTAGGTTGGTTCGGCTTTAACGGTGGCAGTACT
TTAGCTGCTGACCCATCACTAGTACCATATGTAATTGGAACAACCTTATTATCTACTTCTGCCGCATTAGTGTCATCCTC
TTTATATACAAAATTACGATTTAAGAAAGTAGATGCATCATTATCCATGAATGGTGTACTGGGTGGATTAGTAGGTATTA
CTGCAGGTGCCGCAGAAATTTCTCTTGGGGGATCAATTATTGTCGGTCTAATTTCTGGTGTAGTACTTGTAGAAGGAATT
CGATTCTTAGATACAAAATTACGTATAGACGACCCAGTTGGAGCTATTGCAGTACATGGAATTTGTGGTATATGGGGAAC
ATTAGCCATAGGATTGTTCTCTGTTAATACCGGACTTTTCTACGGAAATGGTATTTCTCAATTGGGGATTCAAGCCATCG
GTGTACTTGCTGTAGTTGCTTGGGTTGCTATTACAGCTGGTGGGTTCACTTATGTGATAAATAAAATATCTCAAATTCGC
GTTTCAGAAGAAGAAGAAATTTCTGGACTTGATTTTGCAGAACACGGATCTAGTGCATATAATTTTAAAGAGAGTTTACT
TGAAGGAACGGACAATAATTCATCAAGTGGTCTTGCAGAACGTTTAAATAATAATATTCCGATTGGTAAAACAAGTAACC
AAAGTTCTTAA

Upstream 100 bases:

>100_bases
GTAAAATTTTTGTCTCTCCTGTAGAAGAAGTTATTCGAATTCGTACTGGCGAACGTGGTATGGAAGCTGTTTTATAAATT
ATAAAGGGGGATAACAAAAC

Downstream 100 bases:

>100_bases
ATCAGTCATTAACCAATAAATATCAATACCATATTGATGTTTAAATTATAGGAAAACGCAACATTCGGATTTCATAAAGA
ACAACCAATTAAAAAACCGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLKKSFLIFTALLLLATPVYAATPTPESLELSVNLIWVMLGAFLVFFMHAGFAMVESGFTRSKNTLNILMKNFLTISVAS
ILFFLVGYGIMFGSSAGSIIGSDGFLLSEISEIDFFVFQAMFVATCATIISGAVAERIKLGSYILIVAAMTTIIYPVVGH
WVWQGDGWLTSLGFSDFAGSTVVHLTGAVGAFVVVLILGPRIGKYKGKKVNVIQGHNLPLGALGVFILWLGWFGFNGGST
LAADPSLVPYVIGTTLLSTSAALVSSSLYTKLRFKKVDASLSMNGVLGGLVGITAGAAEISLGGSIIVGLISGVVLVEGI
RFLDTKLRIDDPVGAIAVHGICGIWGTLAIGLFSVNTGLFYGNGISQLGIQAIGVLAVVAWVAITAGGFTYVINKISQIR
VSEEEEISGLDFAEHGSSAYNFKESLLEGTDNNSSSGLAERLNNNIPIGKTSNQSS

Sequences:

>Translated_456_residues
MLKKSFLIFTALLLLATPVYAATPTPESLELSVNLIWVMLGAFLVFFMHAGFAMVESGFTRSKNTLNILMKNFLTISVAS
ILFFLVGYGIMFGSSAGSIIGSDGFLLSEISEIDFFVFQAMFVATCATIISGAVAERIKLGSYILIVAAMTTIIYPVVGH
WVWQGDGWLTSLGFSDFAGSTVVHLTGAVGAFVVVLILGPRIGKYKGKKVNVIQGHNLPLGALGVFILWLGWFGFNGGST
LAADPSLVPYVIGTTLLSTSAALVSSSLYTKLRFKKVDASLSMNGVLGGLVGITAGAAEISLGGSIIVGLISGVVLVEGI
RFLDTKLRIDDPVGAIAVHGICGIWGTLAIGLFSVNTGLFYGNGISQLGIQAIGVLAVVAWVAITAGGFTYVINKISQIR
VSEEEEISGLDFAEHGSSAYNFKESLLEGTDNNSSSGLAERLNNNIPIGKTSNQSS
>Mature_456_residues
MLKKSFLIFTALLLLATPVYAATPTPESLELSVNLIWVMLGAFLVFFMHAGFAMVESGFTRSKNTLNILMKNFLTISVAS
ILFFLVGYGIMFGSSAGSIIGSDGFLLSEISEIDFFVFQAMFVATCATIISGAVAERIKLGSYILIVAAMTTIIYPVVGH
WVWQGDGWLTSLGFSDFAGSTVVHLTGAVGAFVVVLILGPRIGKYKGKKVNVIQGHNLPLGALGVFILWLGWFGFNGGST
LAADPSLVPYVIGTTLLSTSAALVSSSLYTKLRFKKVDASLSMNGVLGGLVGITAGAAEISLGGSIIVGLISGVVLVEGI
RFLDTKLRIDDPVGAIAVHGICGIWGTLAIGLFSVNTGLFYGNGISQLGIQAIGVLAVVAWVAITAGGFTYVINKISQIR
VSEEEEISGLDFAEHGSSAYNFKESLLEGTDNNSSSGLAERLNNNIPIGKTSNQSS

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=416, Percent_Identity=30.5288461538462, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17550180, Length=456, Percent_Identity=34.2105263157895, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17550182, Length=461, Percent_Identity=32.1041214750542, Blast_Score=231, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI32564042, Length=443, Percent_Identity=29.5711060948081, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI133931100, Length=432, Percent_Identity=30.0925925925926, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324187, Length=442, Percent_Identity=27.1493212669683, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=29.8076923076923, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6321559, Length=438, Percent_Identity=29.2237442922374, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI161078314, Length=420, Percent_Identity=37.1428571428571, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47954; Mature: 47954

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKSFLIFTALLLLATPVYAATPTPESLELSVNLIWVMLGAFLVFFMHAGFAMVESGFT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
RSKNTLNILMKNFLTISVASILFFLVGYGIMFGSSAGSIIGSDGFLLSEISEIDFFVFQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
MFVATCATIISGAVAERIKLGSYILIVAAMTTIIYPVVGHWVWQGDGWLTSLGFSDFAGS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCCCCCCCC
TVVHLTGAVGAFVVVLILGPRIGKYKGKKVNVIQGHNLPLGALGVFILWLGWFGFNGGST
EEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
LAADPSLVPYVIGTTLLSTSAALVSSSLYTKLRFKKVDASLSMNGVLGGLVGITAGAAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHE
SLGGSIIVGLISGVVLVEGIRFLDTKLRIDDPVGAIAVHGICGIWGTLAIGLFSVNTGLF
ECCHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
YGNGISQLGIQAIGVLAVVAWVAITAGGFTYVINKISQIRVSEEEEISGLDFAEHGSSAY
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCHH
NFKESLLEGTDNNSSSGLAERLNNNIPIGKTSNQSS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKKSFLIFTALLLLATPVYAATPTPESLELSVNLIWVMLGAFLVFFMHAGFAMVESGFT
CCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
RSKNTLNILMKNFLTISVASILFFLVGYGIMFGSSAGSIIGSDGFLLSEISEIDFFVFQA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
MFVATCATIISGAVAERIKLGSYILIVAAMTTIIYPVVGHWVWQGDGWLTSLGFSDFAGS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCEEECCCCEEEECCCCCCCCC
TVVHLTGAVGAFVVVLILGPRIGKYKGKKVNVIQGHNLPLGALGVFILWLGWFGFNGGST
EEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
LAADPSLVPYVIGTTLLSTSAALVSSSLYTKLRFKKVDASLSMNGVLGGLVGITAGAAEI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHE
SLGGSIIVGLISGVVLVEGIRFLDTKLRIDDPVGAIAVHGICGIWGTLAIGLFSVNTGLF
ECCHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
YGNGISQLGIQAIGVLAVVAWVAITAGGFTYVINKISQIRVSEEEEISGLDFAEHGSSAY
ECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCHH
NFKESLLEGTDNNSSSGLAERLNNNIPIGKTSNQSS
HHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]