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The map label for this gene is glnB [H]

Identifier: 23098667

GI number: 23098667

Start: 1261869

End: 1262210

Strand: Reverse

Name: glnB [H]

Synonym: OB1212

Alternate gene names: 23098667

Gene position: 1262210-1261869 (Counterclockwise)

Preceding gene: 23098672

Following gene: 23098666

Centisome position: 34.77

GC content: 34.8

Gene sequence:

>342_bases
ATGAAAAAATTGGAAGCAATTATACGTCCAGAAAAATTCCAGTCTCTTCGTGATGAGTTATCTTCAATTGGAATTGGTGG
TTTAACTGTTACTGAAGTAGCAGGTACAGGAAAGCAAAAAGGCCAAACAGGGGTTTATCGCTCGACAGAGTTTCAAATTC
AATTATTATCCAAATTGAAAGTAGAGATGGTGATTGAAGAAAGTCAATTAGAAGAAATTGTTGATATTATCATTAAAAGC
TGTAAGACAGGTAATATAGGAGACGGTAAAATTTTTGTCTCTCCTGTAGAAGAAGTTATTCGAATTCGTACTGGCGAACG
TGGTATGGAAGCTGTTTTATAA

Upstream 100 bases:

>100_bases
CAAAGTAAAAAACACTTGCAAAACACTTATAAAACATTTATGATAATTTACATAACCAAAGATGTTAGGAAATCTAACAT
TAATATTAAGGGGTGGAGTA

Downstream 100 bases:

>100_bases
ATTATAAAGGGGGATAACAAAACATGTTGAAGAAAAGTTTTCTTATATTTACAGCACTGTTACTTTTAGCTACTCCAGTT
TACGCAGCGACACCTACACC

Product: nitrogen regulatory PII protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 113; Mature: 113

Protein sequence:

>113_residues
MKKLEAIIRPEKFQSLRDELSSIGIGGLTVTEVAGTGKQKGQTGVYRSTEFQIQLLSKLKVEMVIEESQLEEIVDIIIKS
CKTGNIGDGKIFVSPVEEVIRIRTGERGMEAVL

Sequences:

>Translated_113_residues
MKKLEAIIRPEKFQSLRDELSSIGIGGLTVTEVAGTGKQKGQTGVYRSTEFQIQLLSKLKVEMVIEESQLEEIVDIIIKS
CKTGNIGDGKIFVSPVEEVIRIRTGERGMEAVL
>Mature_113_residues
MKKLEAIIRPEKFQSLRDELSSIGIGGLTVTEVAGTGKQKGQTGVYRSTEFQIQLLSKLKVEMVIEESQLEEIVDIIIKS
CKTGNIGDGKIFVSPVEEVIRIRTGERGMEAVL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=106, Percent_Identity=52.8301886792453, Blast_Score=119, Evalue=6e-29,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=50, Blast_Score=116, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12465; Mature: 12465

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLEAIIRPEKFQSLRDELSSIGIGGLTVTEVAGTGKQKGQTGVYRSTEFQIQLLSKLK
CCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
VEMVIEESQLEEIVDIIIKSCKTGNIGDGKIFVSPVEEVIRIRTGERGMEAVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MKKLEAIIRPEKFQSLRDELSSIGIGGLTVTEVAGTGKQKGQTGVYRSTEFQIQLLSKLK
CCCHHHHCCCHHHHHHHHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
VEMVIEESQLEEIVDIIIKSCKTGNIGDGKIFVSPVEEVIRIRTGERGMEAVL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]