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The map label for this gene is pbpF [H]

Identifier: 23098621

GI number: 23098621

Start: 1214497

End: 1216668

Strand: Direct

Name: pbpF [H]

Synonym: OB1166

Alternate gene names: 23098621

Gene position: 1214497-1216668 (Clockwise)

Preceding gene: 23098619

Following gene: 23098622

Centisome position: 33.45

GC content: 36.42

Gene sequence:

>2172_bases
ATGAGAATGGAAAAGCGTAAGCAAAATAAGTTAGCTTACAAAAAGAAAACTCAATGGTTTTTCATTGCAATTGCCTCTCT
TTTTATATTACTACTTCTTGGATATGGTGTCGTTTTATTAGGTGGAAAAATTATAGCAAATGATGAAGAATTGATTCTCG
ATTTAAAAACGACAATACGAACAGAAGAAGGAGAAGTAATTGGGACATTATATAAAGAAAATAGGGAGTTTACAAATCTA
GACAGTCTACCAGATCATGTGAAAGAAGCTTTTATCGCGATTGAAGACAGGCGTTTTTATGAACATCAAGGAATTGATCT
TAAATCAATTAGCAGAGCTGTATACCGTGATATTATTGCGATGAGCAAAGTTGAAGGTGGAAGTACGATTACACAACAAC
TTGCTAAAAATTTATTTTTGAGCAATGACAAAACATGGTCGAGGAAAATAAAAGAGACAATGGCAGCCTTATATTTAGAA
AGGGAATTATCAAAGGATGAAATTCTTGAGTTATATGTGAATGAAGTTTACTTCGGTCAAGGTATTTATGGTATTCAAAC
TGCCTCAAAGTATTTCTTTTCCAAATCAGCTGATGAATTAACTGTAGCTGAAGGAGCAATGCTTGCAGGATTAATGAAAG
GTCCAAACGGGTATTCTCCCATTAATCATCCAGATCGTGCGCTAGAACGAAGGAATGTTGTTTTACATTCAATGGAAGAT
GCAGGATTTTTGAGTACTGAACAACGAGTTGCGGAGGAAGAAAAAACTTTAGGTATTGCTGTGCAAGAACCGAAAGCTTC
TGATCCATGGGTGGATAGTTATATTGATCTCGTAATGAAAGAGGCTTCAGAGAAACATCAACTTTCTGTTGAAGCACTAC
AAACGGGTGGGTATGACATTACTGTTTATATGGACCCTGAAATTCAACAGATTGCATATTCAGAAATGCAAAACGATGCA
TATTTCCCTGGAAATACGGAAGGTGTGGAAGGAGCATTTGTGATGTTAGATGCTACTAATGCTCGGGTTGTTTCTGCAAT
TGGTGGGAGAGATTACCAAATTGGAGATTTAAATCGAACTGTAGTTAATCGTCAACCTGGTTCCACTTTTAAACCTATAG
CTGTATATGGACCTGCATTAATGCAGGGGGATGTATATACTCCTTTCACTTTGATTCCAGATAAAAAATCGGAAGAATAT
AATGTTGTCAATGCAAATGATGAGTATAAAGGTGAAATTCCTATTTATCAAGCCATTATGGAGTCGACCAATACTTCGGC
TGTTTGGTTATTGGATGAGCTTGGAATTGACTACTCGAAGGGTTATTTGGAAAAACTAGGTATTAGTCTCGAGGATGATG
GTTTATCTATAGCATTAGGTGGTCTTAAGTATGGAGTAACTCCTTTAAAAATGGCGCAGAGCTATCAGGCTTTTGCCTCC
GGTGGTGATTTAACCCGAGCAACATCGATATCCAGTATTGAAAATCGAAACGAAGAAGTGATATATGAATCAACGTTAGA
GAGGGAAAATGTATTTACTAGTCAAGTCGCTTGGAATATGACAGAAATGTTATCTGATACTGTGCTATCTGGAACGGCTT
CTTCTGGTGAGTTTACTAAAGCACTCGCAGGAAAGACTGGATCTACACAACATACAATTGTAGATGGTGCTACGAAAGAT
GCTTGGTTTGTTGGATATACTCCTGAATATGTAAGTGCTATGTGGATGGGGTATGATAGCACAGATAAAGATCATTACTT
AACAGGCGGTAGTTCTTATCCTACATCTTTAACAAAGAAAATTTTAACCGAAATAGATAGGCAAACAGATCGAGAAATGT
CGAGCAGTTTTGAAATACCTGAGAATGTTGAACCACTTCCAGAACCAATTGAATTACCTACAATTTCGGATATTACCGCC
AACTATGAATTTGGAGGATTTTCGATAATCAAAGGAAATCTTAATTGGAATGGTTCATCTGATGAACGTGTTGTTTATCG
TATTTATCAAGAGTCAGAAGGTGAAGATAAACTTATTGATGCAGTAAAAGGAGTAACCGAATATACAATTGACAATGTTT
GGTTTAAAAACAATAAATATTACGTTGTTCCTTACGATCCGAACACAAAAATGGAAGGAACCAAGTCAGATTCTGTGACG
TTTAGTTGGTAA

Upstream 100 bases:

>100_bases
ACATATTAATTAAAATGATTATTAATTAATAGAGACTATATTCTTTAATATTGACGAATGATGACGTATAATGTAAATGT
ACACAGCTAGAGGTGAAGTT

Downstream 100 bases:

>100_bases
AAATGAGGCAAATTGATGACAAACACCTGCCTTCCCTATACACTCAAGTATTTAATAGCTATAGTAGACTATGAAGATAA
GAGAATATAAAGGATGAATG

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 723; Mature: 723

Protein sequence:

>723_residues
MRMEKRKQNKLAYKKKTQWFFIAIASLFILLLLGYGVVLLGGKIIANDEELILDLKTTIRTEEGEVIGTLYKENREFTNL
DSLPDHVKEAFIAIEDRRFYEHQGIDLKSISRAVYRDIIAMSKVEGGSTITQQLAKNLFLSNDKTWSRKIKETMAALYLE
RELSKDEILELYVNEVYFGQGIYGIQTASKYFFSKSADELTVAEGAMLAGLMKGPNGYSPINHPDRALERRNVVLHSMED
AGFLSTEQRVAEEEKTLGIAVQEPKASDPWVDSYIDLVMKEASEKHQLSVEALQTGGYDITVYMDPEIQQIAYSEMQNDA
YFPGNTEGVEGAFVMLDATNARVVSAIGGRDYQIGDLNRTVVNRQPGSTFKPIAVYGPALMQGDVYTPFTLIPDKKSEEY
NVVNANDEYKGEIPIYQAIMESTNTSAVWLLDELGIDYSKGYLEKLGISLEDDGLSIALGGLKYGVTPLKMAQSYQAFAS
GGDLTRATSISSIENRNEEVIYESTLERENVFTSQVAWNMTEMLSDTVLSGTASSGEFTKALAGKTGSTQHTIVDGATKD
AWFVGYTPEYVSAMWMGYDSTDKDHYLTGGSSYPTSLTKKILTEIDRQTDREMSSSFEIPENVEPLPEPIELPTISDITA
NYEFGGFSIIKGNLNWNGSSDERVVYRIYQESEGEDKLIDAVKGVTEYTIDNVWFKNNKYYVVPYDPNTKMEGTKSDSVT
FSW

Sequences:

>Translated_723_residues
MRMEKRKQNKLAYKKKTQWFFIAIASLFILLLLGYGVVLLGGKIIANDEELILDLKTTIRTEEGEVIGTLYKENREFTNL
DSLPDHVKEAFIAIEDRRFYEHQGIDLKSISRAVYRDIIAMSKVEGGSTITQQLAKNLFLSNDKTWSRKIKETMAALYLE
RELSKDEILELYVNEVYFGQGIYGIQTASKYFFSKSADELTVAEGAMLAGLMKGPNGYSPINHPDRALERRNVVLHSMED
AGFLSTEQRVAEEEKTLGIAVQEPKASDPWVDSYIDLVMKEASEKHQLSVEALQTGGYDITVYMDPEIQQIAYSEMQNDA
YFPGNTEGVEGAFVMLDATNARVVSAIGGRDYQIGDLNRTVVNRQPGSTFKPIAVYGPALMQGDVYTPFTLIPDKKSEEY
NVVNANDEYKGEIPIYQAIMESTNTSAVWLLDELGIDYSKGYLEKLGISLEDDGLSIALGGLKYGVTPLKMAQSYQAFAS
GGDLTRATSISSIENRNEEVIYESTLERENVFTSQVAWNMTEMLSDTVLSGTASSGEFTKALAGKTGSTQHTIVDGATKD
AWFVGYTPEYVSAMWMGYDSTDKDHYLTGGSSYPTSLTKKILTEIDRQTDREMSSSFEIPENVEPLPEPIELPTISDITA
NYEFGGFSIIKGNLNWNGSSDERVVYRIYQESEGEDKLIDAVKGVTEYTIDNVWFKNNKYYVVPYDPNTKMEGTKSDSVT
FSW
>Mature_723_residues
MRMEKRKQNKLAYKKKTQWFFIAIASLFILLLLGYGVVLLGGKIIANDEELILDLKTTIRTEEGEVIGTLYKENREFTNL
DSLPDHVKEAFIAIEDRRFYEHQGIDLKSISRAVYRDIIAMSKVEGGSTITQQLAKNLFLSNDKTWSRKIKETMAALYLE
RELSKDEILELYVNEVYFGQGIYGIQTASKYFFSKSADELTVAEGAMLAGLMKGPNGYSPINHPDRALERRNVVLHSMED
AGFLSTEQRVAEEEKTLGIAVQEPKASDPWVDSYIDLVMKEASEKHQLSVEALQTGGYDITVYMDPEIQQIAYSEMQNDA
YFPGNTEGVEGAFVMLDATNARVVSAIGGRDYQIGDLNRTVVNRQPGSTFKPIAVYGPALMQGDVYTPFTLIPDKKSEEY
NVVNANDEYKGEIPIYQAIMESTNTSAVWLLDELGIDYSKGYLEKLGISLEDDGLSIALGGLKYGVTPLKMAQSYQAFAS
GGDLTRATSISSIENRNEEVIYESTLERENVFTSQVAWNMTEMLSDTVLSGTASSGEFTKALAGKTGSTQHTIVDGATKD
AWFVGYTPEYVSAMWMGYDSTDKDHYLTGGSSYPTSLTKKILTEIDRQTDREMSSSFEIPENVEPLPEPIELPTISDITA
NYEFGGFSIIKGNLNWNGSSDERVVYRIYQESEGEDKLIDAVKGVTEYTIDNVWFKNNKYYVVPYDPNTKMEGTKSDSVT
FSW

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=545, Percent_Identity=31.9266055045872, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=35.5048859934853, Blast_Score=182, Evalue=9e-47,
Organism=Escherichia coli, GI1788867, Length=637, Percent_Identity=27.6295133437991, Blast_Score=177, Evalue=3e-45,
Organism=Escherichia coli, GI1789601, Length=172, Percent_Identity=33.7209302325581, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 80925; Mature: 80925

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMEKRKQNKLAYKKKTQWFFIAIASLFILLLLGYGVVLLGGKIIANDEELILDLKTTIR
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEHHHEE
TEEGEVIGTLYKENREFTNLDSLPDHVKEAFIAIEDRRFYEHQGIDLKSISRAVYRDIIA
CCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHEEEECCHHHHHCCCCHHHHHHHHHHHHHH
MSKVEGGSTITQQLAKNLFLSNDKTWSRKIKETMAALYLERELSKDEILELYVNEVYFGQ
HHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECC
GIYGIQTASKYFFSKSADELTVAEGAMLAGLMKGPNGYSPINHPDRALERRNVVLHSMED
CEEEHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECCC
AGFLSTEQRVAEEEKTLGIAVQEPKASDPWVDSYIDLVMKEASEKHQLSVEALQTGGYDI
CCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEHHHHHCCCEEE
TVYMDPEIQQIAYSEMQNDAYFPGNTEGVEGAFVMLDATNARVVSAIGGRDYQIGDLNRT
EEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEECCCCHH
VVNRQPGSTFKPIAVYGPALMQGDVYTPFTLIPDKKSEEYNVVNANDEYKGEIPIYQAIM
EECCCCCCCCCCEEEECCHHCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHH
ESTNTSAVWLLDELGIDYSKGYLEKLGISLEDDGLSIALGGLKYGVTPLKMAQSYQAFAS
HCCCCCEEEEEHHHCCCCCCCHHHHCCCEECCCCCEEEECCHHHCCCHHHHHHHHHHHHC
GGDLTRATSISSIENRNEEVIYESTLERENVFTSQVAWNMTEMLSDTVLSGTASSGEFTK
CCCCCHHHCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ALAGKTGSTQHTIVDGATKDAWFVGYTPEYVSAMWMGYDSTDKDHYLTGGSSYPTSLTKK
HHCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHH
ILTEIDRQTDREMSSSFEIPENVEPLPEPIELPTISDITANYEFGGFSIIKGNLNWNGSS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
DERVVYRIYQESEGEDKLIDAVKGVTEYTIDNVWFKNNKYYVVPYDPNTKMEGTKSDSVT
CCEEEEEEEECCCCHHHHHHHHHHHHHHEECCEEEECCEEEEEEECCCCCCCCCCCCCEE
FSW
EEC
>Mature Secondary Structure
MRMEKRKQNKLAYKKKTQWFFIAIASLFILLLLGYGVVLLGGKIIANDEELILDLKTTIR
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEHHHEE
TEEGEVIGTLYKENREFTNLDSLPDHVKEAFIAIEDRRFYEHQGIDLKSISRAVYRDIIA
CCCCCEEEEHHHCCCCCCCCCCCHHHHHHHHEEEECCHHHHHCCCCHHHHHHHHHHHHHH
MSKVEGGSTITQQLAKNLFLSNDKTWSRKIKETMAALYLERELSKDEILELYVNEVYFGQ
HHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECC
GIYGIQTASKYFFSKSADELTVAEGAMLAGLMKGPNGYSPINHPDRALERRNVVLHSMED
CEEEHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECCC
AGFLSTEQRVAEEEKTLGIAVQEPKASDPWVDSYIDLVMKEASEKHQLSVEALQTGGYDI
CCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHEEHHHHHCCCEEE
TVYMDPEIQQIAYSEMQNDAYFPGNTEGVEGAFVMLDATNARVVSAIGGRDYQIGDLNRT
EEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEECCCCHH
VVNRQPGSTFKPIAVYGPALMQGDVYTPFTLIPDKKSEEYNVVNANDEYKGEIPIYQAIM
EECCCCCCCCCCEEEECCHHCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCHHHHHHH
ESTNTSAVWLLDELGIDYSKGYLEKLGISLEDDGLSIALGGLKYGVTPLKMAQSYQAFAS
HCCCCCEEEEEHHHCCCCCCCHHHHCCCEECCCCCEEEECCHHHCCCHHHHHHHHHHHHC
GGDLTRATSISSIENRNEEVIYESTLERENVFTSQVAWNMTEMLSDTVLSGTASSGEFTK
CCCCCHHHCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
ALAGKTGSTQHTIVDGATKDAWFVGYTPEYVSAMWMGYDSTDKDHYLTGGSSYPTSLTKK
HHCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHH
ILTEIDRQTDREMSSSFEIPENVEPLPEPIELPTISDITANYEFGGFSIIKGNLNWNGSS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
DERVVYRIYQESEGEDKLIDAVKGVTEYTIDNVWFKNNKYYVVPYDPNTKMEGTKSDSVT
CCEEEEEEEECCCCHHHHHHHHHHHHHHEECCEEEECCEEEEEEECCCCCCCCCCCCCEE
FSW
EEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]