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The map label for this gene is spsI [H]

Identifier: 23098584

GI number: 23098584

Start: 1179905

End: 1180624

Strand: Reverse

Name: spsI [H]

Synonym: OB1129

Alternate gene names: 23098584

Gene position: 1180624-1179905 (Counterclockwise)

Preceding gene: 23098586

Following gene: 23098583

Centisome position: 32.52

GC content: 33.75

Gene sequence:

>720_bases
ATGAAGGGCATGATACTTGCCAGAGGAAAAGGAACCAGACTTCACCCTCTTACAAAAGTGATAAATAAACATTTGATTCC
TATAGGTAAATATCCAATGATTTACTATCCGATATTTAAATTACGCGAAGTTGATATAACAGAAATTTTAATCACTACCA
ATCAACAAGATATACCACTTTTTCGAGAATTATTAGGTGACGGAAGAGACTTAGGTGTACGTATTCACTTCGAAGCACAG
CAGCAGGCTATCGGTATACCAGACGCAATATTACTTAGTAAAACATTTGTTGGCAGTGATAAATTAACGGTTATACTGGG
TGACAATATATTTCATGATTCCTTAATTCCTTATGTCCAGTCATATGAGAAGCAAAGCACCGGAGCCAAAATTTTGTTGA
AAACCGTTTCGGATCCTAATCGATATGGAATTGCTACATTTGATAAGTCTCAAAAAAATATATTATCCATTGAAGAAAAA
CCAAGTGATTCTTCTTCTAATTATTGTGTCACAGGAATTTATATGTACGGTACGGAAGTTTTTGAATTTATAAAACAAAT
TACTCCTTCTTCTCGAGGAGAGTTAGAGATTACAGATGTGAACAATTTATACTTACAAAAGAGCAAACTTACTTTTGATG
TATTACCGAATTGGTGGATCGACGCTGGGACATATGATTCCATCTTTCAAGCAAATCAAATTATTCTTAAAAACGAATAG

Upstream 100 bases:

>100_bases
GGGGCGTTTGCGCTGGACGTGACTAATTAACTTAGTATGTTTATCCACATCTTTAAATTTTTCAATTTCCTATACAACTG
AGGCGGGAGTGTAATACAAA

Downstream 100 bases:

>100_bases
TAATCTCCAAATGGAAATGGTTGTGGTTTACAATGAGATTTCTGATTACAGGTTGTGAGGGTCAACTTGGGAAAGCGTTC
ATTTCCAAGTTAGGTCTTGG

Product: spore coat polysaccharide synthesis

Products: diphosphate; dTDPglucose

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKGMILARGKGTRLHPLTKVINKHLIPIGKYPMIYYPIFKLREVDITEILITTNQQDIPLFRELLGDGRDLGVRIHFEAQ
QQAIGIPDAILLSKTFVGSDKLTVILGDNIFHDSLIPYVQSYEKQSTGAKILLKTVSDPNRYGIATFDKSQKNILSIEEK
PSDSSSNYCVTGIYMYGTEVFEFIKQITPSSRGELEITDVNNLYLQKSKLTFDVLPNWWIDAGTYDSIFQANQIILKNE

Sequences:

>Translated_239_residues
MKGMILARGKGTRLHPLTKVINKHLIPIGKYPMIYYPIFKLREVDITEILITTNQQDIPLFRELLGDGRDLGVRIHFEAQ
QQAIGIPDAILLSKTFVGSDKLTVILGDNIFHDSLIPYVQSYEKQSTGAKILLKTVSDPNRYGIATFDKSQKNILSIEEK
PSDSSSNYCVTGIYMYGTEVFEFIKQITPSSRGELEITDVNNLYLQKSKLTFDVLPNWWIDAGTYDSIFQANQIILKNE
>Mature_239_residues
MKGMILARGKGTRLHPLTKVINKHLIPIGKYPMIYYPIFKLREVDITEILITTNQQDIPLFRELLGDGRDLGVRIHFEAQ
QQAIGIPDAILLSKTFVGSDKLTVILGDNIFHDSLIPYVQSYEKQSTGAKILLKTVSDPNRYGIATFDKSQKNILSIEEK
PSDSSSNYCVTGIYMYGTEVFEFIKQITPSSRGELEITDVNNLYLQKSKLTFDVLPNWWIDAGTYDSIFQANQIILKNE

Specific function: Catalyzes The Formation Of Dtdp-Glucose, From Dttp And Glucose 1-Phosphate, As Well As Its Pyrophosphorolysis. [C]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=225, Percent_Identity=24.8888888888889, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI11761619, Length=225, Percent_Identity=24.8888888888889, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788351, Length=237, Percent_Identity=41.3502109704641, Blast_Score=201, Evalue=3e-53,
Organism=Escherichia coli, GI1790224, Length=238, Percent_Identity=41.5966386554622, Blast_Score=199, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI133931050, Length=243, Percent_Identity=25.9259259259259, Blast_Score=90, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6320148, Length=226, Percent_Identity=27.4336283185841, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355443, Length=228, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644084, Length=228, Percent_Identity=25.4385964912281, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: 2.7.7.24

Molecular weight: Translated: 27108; Mature: 27108

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMILARGKGTRLHPLTKVINKHLIPIGKYPMIYYPIFKLREVDITEILITTNQQDIPL
CCCEEEECCCCCEEHHHHHHHHHHCCCCCCCCEEEEEHEEEEECCEEEEEEECCCCCCHH
FRELLGDGRDLGVRIHFEAQQQAIGIPDAILLSKTFVGSDKLTVILGDNIFHDSLIPYVQ
HHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHEEECCCCEEEEECCCCCHHHHHHHHH
SYEKQSTGAKILLKTVSDPNRYGIATFDKSQKNILSIEEKPSDSSSNYCVTGIYMYGTEV
HHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEEHHHHH
FEFIKQITPSSRGELEITDVNNLYLQKSKLTFDVLPNWWIDAGTYDSIFQANQIILKNE
HHHHHHHCCCCCCCEEEEECCCEEEEECCEEEEECCCCEECCCCCHHHHCCCEEEEECC
>Mature Secondary Structure
MKGMILARGKGTRLHPLTKVINKHLIPIGKYPMIYYPIFKLREVDITEILITTNQQDIPL
CCCEEEECCCCCEEHHHHHHHHHHCCCCCCCCEEEEEHEEEEECCEEEEEEECCCCCCHH
FRELLGDGRDLGVRIHFEAQQQAIGIPDAILLSKTFVGSDKLTVILGDNIFHDSLIPYVQ
HHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHEEECCCCEEEEECCCCCHHHHHHHHH
SYEKQSTGAKILLKTVSDPNRYGIATFDKSQKNILSIEEKPSDSSSNYCVTGIYMYGTEV
HHHCCCCCCEEEEEECCCCCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEEEHHHHH
FEFIKQITPSSRGELEITDVNNLYLQKSKLTFDVLPNWWIDAGTYDSIFQANQIILKNE
HHHHHHHCCCCCCCEEEEECCCEEEEECCEEEEECCCCEECCCCCHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTTP; alpha-D-glucose 1-phosphate

Specific reaction: dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]