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The map label for this gene is spsK [H]

Identifier: 23098583

GI number: 23098583

Start: 1179036

End: 1179884

Strand: Reverse

Name: spsK [H]

Synonym: OB1128

Alternate gene names: 23098583

Gene position: 1179884-1179036 (Counterclockwise)

Preceding gene: 23098584

Following gene: 23098582

Centisome position: 32.5

GC content: 35.45

Gene sequence:

>849_bases
TTGTGGTTTACAATGAGATTTCTGATTACAGGTTGTGAGGGTCAACTTGGGAAAGCGTTCATTTCCAAGTTAGGTCTTGG
TATTAACATAAAGCGCCTCTCAAAAAAAGAGATGGATATAACCGATCCAATTAAAATTGAACAACAAATACAATCCTTTC
AACCAGACTACGTTATCCATACTGCTGCATATACAGCAGTTGATCTTAGCGAAAAACACCCAATTCTTGCTCTACAAGTA
AATGCGATTGGGACATTACATCTTGCTCGTGCTTGTAAAAAATACGGAGCAAAGCTTGTTTTTTTTAGTTCTGATTACGT
ATTTGATGGAGAAAAAAACACCCCTTATATTGAATCCGATCGTCCAAATCCCAAAAATAATTATGGTTTAAGTAAGTGGC
TAGCAGAGGAGTTTATTCTACAAACATTACCAGAGAGTTATATTATACGAACATCATGGTTGTTTGGGGATGGCGAAAAT
AACTTTGTTAATACGATTAAAAAAAATGCCTACAAAAGAAAACCATTAAAAGTTATCAATGATCAAATTGGGAGTCCGAC
CTATACCTATGATTTAGTTGAAGCTTGCATACCATTGCTGCAACTTCCTTTTGGTATTTACCATATACGAAATGATGGTA
TTTGTAGTTGGTACTCCTTTGCACAAACAATTTATGAGGAATGCGGAACTGACCCAACTTTAATTACACCTGTTACGTCT
AAGGAATATAAAACTCTAGCAAAGAGACCGAGTTACTCTGTTTTAAGTATGAATAAGCTGAAATCCTCTGGAACTAAACT
CCCTCGTTTTTGGAAAGAAGCCCTTTGTAACTTTATTAATAAGGAGTAG

Upstream 100 bases:

>100_bases
TATTACCGAATTGGTGGATCGACGCTGGGACATATGATTCCATCTTTCAAGCAAATCAAATTATTCTTAAAAACGAATAG
TAATCTCCAAATGGAAATGG

Downstream 100 bases:

>100_bases
ATATATTTGATTGAAGGTGTACAGATAAAACGAATAGAAAAACACTGTGACGATCGCGGATATCTAGCTGAATTAGTAAG
AGACGATGAACATTTATTAT

Product: spore coat polysaccharide synthesis

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MWFTMRFLITGCEGQLGKAFISKLGLGINIKRLSKKEMDITDPIKIEQQIQSFQPDYVIHTAAYTAVDLSEKHPILALQV
NAIGTLHLARACKKYGAKLVFFSSDYVFDGEKNTPYIESDRPNPKNNYGLSKWLAEEFILQTLPESYIIRTSWLFGDGEN
NFVNTIKKNAYKRKPLKVINDQIGSPTYTYDLVEACIPLLQLPFGIYHIRNDGICSWYSFAQTIYEECGTDPTLITPVTS
KEYKTLAKRPSYSVLSMNKLKSSGTKLPRFWKEALCNFINKE

Sequences:

>Translated_282_residues
MWFTMRFLITGCEGQLGKAFISKLGLGINIKRLSKKEMDITDPIKIEQQIQSFQPDYVIHTAAYTAVDLSEKHPILALQV
NAIGTLHLARACKKYGAKLVFFSSDYVFDGEKNTPYIESDRPNPKNNYGLSKWLAEEFILQTLPESYIIRTSWLFGDGEN
NFVNTIKKNAYKRKPLKVINDQIGSPTYTYDLVEACIPLLQLPFGIYHIRNDGICSWYSFAQTIYEECGTDPTLITPVTS
KEYKTLAKRPSYSVLSMNKLKSSGTKLPRFWKEALCNFINKE
>Mature_282_residues
MWFTMRFLITGCEGQLGKAFISKLGLGINIKRLSKKEMDITDPIKIEQQIQSFQPDYVIHTAAYTAVDLSEKHPILALQV
NAIGTLHLARACKKYGAKLVFFSSDYVFDGEKNTPYIESDRPNPKNNYGLSKWLAEEFILQTLPESYIIRTSWLFGDGEN
NFVNTIKKNAYKRKPLKVINDQIGSPTYTYDLVEACIPLLQLPFGIYHIRNDGICSWYSFAQTIYEECGTDPTLITPVTS
KEYKTLAKRPSYSVLSMNKLKSSGTKLPRFWKEALCNFINKE

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=277, Percent_Identity=27.7978339350181, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI33519455, Length=277, Percent_Identity=27.7978339350181, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1788352, Length=292, Percent_Identity=30.1369863013699, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32214; Mature: 32214

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWFTMRFLITGCEGQLGKAFISKLGLGINIKRLSKKEMDITDPIKIEQQIQSFQPDYVIH
CEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEE
TAAYTAVDLSEKHPILALQVNAIGTLHLARACKKYGAKLVFFSSDYVFDGEKNTPYIESD
EHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCEECCC
RPNPKNNYGLSKWLAEEFILQTLPESYIIRTSWLFGDGENNFVNTIKKNAYKRKPLKVIN
CCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHH
DQIGSPTYTYDLVEACIPLLQLPFGIYHIRNDGICSWYSFAQTIYEECGTDPTLITPVTS
HHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
KEYKTLAKRPSYSVLSMNKLKSSGTKLPRFWKEALCNFINKE
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWFTMRFLITGCEGQLGKAFISKLGLGINIKRLSKKEMDITDPIKIEQQIQSFQPDYVIH
CEEEEEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEE
TAAYTAVDLSEKHPILALQVNAIGTLHLARACKKYGAKLVFFSSDYVFDGEKNTPYIESD
EHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEEECCEEECCCCCCCEECCC
RPNPKNNYGLSKWLAEEFILQTLPESYIIRTSWLFGDGENNFVNTIKKNAYKRKPLKVIN
CCCCCCCCCHHHHHHHHHHHHHCCHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCHHHHH
DQIGSPTYTYDLVEACIPLLQLPFGIYHIRNDGICSWYSFAQTIYEECGTDPTLITPVTS
HHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCC
KEYKTLAKRPSYSVLSMNKLKSSGTKLPRFWKEALCNFINKE
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]