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The map label for this gene is spsL [H]

Identifier: 23098582

GI number: 23098582

Start: 1178574

End: 1179029

Strand: Reverse

Name: spsL [H]

Synonym: OB1127

Alternate gene names: 23098582

Gene position: 1179029-1178574 (Counterclockwise)

Preceding gene: 23098583

Following gene: 23098578

Centisome position: 32.48

GC content: 35.31

Gene sequence:

>456_bases
TTGATTGAAGGTGTACAGATAAAACGAATAGAAAAACACTGTGACGATCGCGGATATCTAGCTGAATTAGTAAGAGACGA
TGAACATTTATTATCGCATTTCGGTCAGGCAACTATTTCCATGAGTTATCCTGGTGTGATTAAAGCATTCCACTATCATG
AAAAACAAGATGATTTATGGTATTTTGCCTCAGGAAACGCTCAAGTCGTTCTATACGACTTGAGAAAAAATACAAATACT
ACTGGCAGAACAGATGTCATCTATATGGGAGAGCACAATCCTATCTTATTACTAATACCAAAGGGGATTGCTCACGGCTA
CAGAGTTTTAGGAAGTAAACCAGCCACAATCATTTATTTTACGACTAAATCTTATAATAAAAATGATCCTGATGAAAAAC
GGTTAGCTTGGAATGACCCAGAAATTAATTTCAATTGGAACACTACTTTCAAATAA

Upstream 100 bases:

>100_bases
CTCTGTTTTAAGTATGAATAAGCTGAAATCCTCTGGAACTAAACTCCCTCGTTTTTGGAAAGAAGCCCTTTGTAACTTTA
TTAATAAGGAGTAGATATAT

Downstream 100 bases:

>100_bases
ATAAGATGCATAAATGGACTGAATGATAATTCTCTTCATAAAAATCCCCTATTATAGGAACGTTTCCTACAATAGGGGAT
TTTTTATTACTTCGTTTTAA

Product: spore coat polysaccharide synthesis

Products: dTDP-4-dehydro-6-deoxy-L-mannose

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MIEGVQIKRIEKHCDDRGYLAELVRDDEHLLSHFGQATISMSYPGVIKAFHYHEKQDDLWYFASGNAQVVLYDLRKNTNT
TGRTDVIYMGEHNPILLLIPKGIAHGYRVLGSKPATIIYFTTKSYNKNDPDEKRLAWNDPEINFNWNTTFK

Sequences:

>Translated_151_residues
MIEGVQIKRIEKHCDDRGYLAELVRDDEHLLSHFGQATISMSYPGVIKAFHYHEKQDDLWYFASGNAQVVLYDLRKNTNT
TGRTDVIYMGEHNPILLLIPKGIAHGYRVLGSKPATIIYFTTKSYNKNDPDEKRLAWNDPEINFNWNTTFK
>Mature_151_residues
MIEGVQIKRIEKHCDDRGYLAELVRDDEHLLSHFGQATISMSYPGVIKAFHYHEKQDDLWYFASGNAQVVLYDLRKNTNT
TGRTDVIYMGEHNPILLLIPKGIAHGYRVLGSKPATIIYFTTKSYNKNDPDEKRLAWNDPEINFNWNTTFK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1898

COG function: function code M; dTDP-4-dehydrorhamnose 3,5-epimerase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To dTDP-4-dehydrorhamnose reductase [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR000888
- InterPro:   IPR014710
- ProDom:   PD001462 [H]

Pfam domain/function: PF00908 dTDP_sugar_isom [H]

EC number: 5.1.3.13

Molecular weight: Translated: 17506; Mature: 17506

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEGVQIKRIEKHCDDRGYLAELVRDDEHLLSHFGQATISMSYPGVIKAFHYHEKQDDLW
CCCCCEEHHHHHCCCCCCHHHHHHCCHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCEE
YFASGNAQVVLYDLRKNTNTTGRTDVIYMGEHNPILLLIPKGIAHGYRVLGSKPATIIYF
EEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEE
TTKSYNKNDPDEKRLAWNDPEINFNWNTTFK
EECCCCCCCCCCCCEECCCCEEEEEECCCCC
>Mature Secondary Structure
MIEGVQIKRIEKHCDDRGYLAELVRDDEHLLSHFGQATISMSYPGVIKAFHYHEKQDDLW
CCCCCEEHHHHHCCCCCCHHHHHHCCHHHHHHHCCCEEEEECCCCCEEEEECCCCCCCEE
YFASGNAQVVLYDLRKNTNTTGRTDVIYMGEHNPILLLIPKGIAHGYRVLGSKPATIIYF
EEECCCCEEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEECCCCCEEEEE
TTKSYNKNDPDEKRLAWNDPEINFNWNTTFK
EECCCCCCCCCCCCEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.025 {dTDP-4-dehydro-6-deoxy-D-glucose}} [C]

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose = dTDP-4-dehydro-6-deoxy-L-mannose

General reaction: Epimerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]