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The map label for this gene is proB

Identifier: 23098507

GI number: 23098507

Start: 1094302

End: 1095420

Strand: Direct

Name: proB

Synonym: OB1052

Alternate gene names: 23098507

Gene position: 1094302-1095420 (Clockwise)

Preceding gene: 23098503

Following gene: 23098508

Centisome position: 30.14

GC content: 37.27

Gene sequence:

>1119_bases
ATGAATAAGAAGAGGGTAGTTATAAAAATTGGAAGTAGCTCACTAACGAATGAAAAAGGTGAAATTGATCATAAGAAACT
AGGAGACCATGTGAATGCGTTAGCTATGTTACATCAACATAACTTTGAAGTTATTTTAGTTTCCTCAGGAGCTGTTGCAG
CGGGTTTTCGAAACTTAGGATATTCTTCTAGACCGGTTACGTTAAAAGGAAAACAAGCATCCGCGGCGATTGGTCAAGGT
CTACTTATTCATACTTATATGGATAAGTTTATGGAATACAATATTCGATCTGCTCAATTATTATTAACGAGGTCTGATTT
TTCGGTCCAAAAACGGTATAAGAATGCTACATCTACTATGCTTGAATTACTAGAACGAGGAGTTATACCGATTATTAATG
AAAACGACACAGTTGCTGTTGATGAGTTAACGTTCGGGGATAATGATATGCTTTCTGCTTTAGTTAGTGGTTCTATCCAT
GCAGCTCAATTAATAATATTAACCGATATTGATGGGATTTATGATAAACATCCTGGTAAATATGCGAGTGCTATGCGGTA
CGATACAATTGATTTAATTACTAATGATATGATACAGGAAACGGATGTTTCAGGATCGAAATTAGGTACTGGTGGTATGA
AATCGAAACTGATGGCAGCAAAGGTAGCAGCTTCTCTCGGAGTCCCAGTTTTTATTGGTAAAGGGGTAGGAGTATCTAAA
TTACTAGAAATAGTAAATGGACATGGACAGGGAACGTATGTTAGAAGTTTCCGCAATAAACAAATTCCGATTCGTAAGCA
ATGGATTTCTTTGCATTCGACATTAGAGGGGAAAGTATTTATTGATGATGGGGCTACTCAAGCACTTATGCATAACGGCG
GTAGTTTGTTGTCAGCTGGTGTGATTAATACTCAGGGAGATTTTGAAGATGGAGATGTTGTAGAAGTTTATAATCGAAGA
AATTTATTAGGAAGAGGGCAAGTGAGTTGTTCGTCTGAAGAGCTCAACGCAGCCAACACATTAAAAAAAGCAAAACAGAT
GACGCAAATCCCTGCTATTGAGGTGATTCACAGAGATTCATGGGTTATGGTTGATCAAATAAAGGAGGAGTTCCAATGA

Upstream 100 bases:

>100_bases
ATTTAGCCCGGTCCAGGACATGCGGTGATAAACGGGCGTTTGGGCTTTTGTTCTTATACCTGAAAATAGACACTTAGAGA
ATATTGAGGAGGAGAAGAAT

Downstream 100 bases:

>100_bases
GTGAAGTAATAGAGAAAGCGAAGCGTGCAAAACAAGCAAGTTTTAAAATGGCTCTATCTACAACGGACGACAAGAATGAA
GCATTGCAATTAATATCACA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNKKRVVIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLGYSSRPVTLKGKQASAAIGQG
LLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTMLELLERGVIPIINENDTVAVDELTFGDNDMLSALVSGSIH
AAQLIILTDIDGIYDKHPGKYASAMRYDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSK
LLEIVNGHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAGVINTQGDFEDGDVVEVYNRR
NLLGRGQVSCSSEELNAANTLKKAKQMTQIPAIEVIHRDSWVMVDQIKEEFQ

Sequences:

>Translated_372_residues
MNKKRVVIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLGYSSRPVTLKGKQASAAIGQG
LLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTMLELLERGVIPIINENDTVAVDELTFGDNDMLSALVSGSIH
AAQLIILTDIDGIYDKHPGKYASAMRYDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSK
LLEIVNGHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAGVINTQGDFEDGDVVEVYNRR
NLLGRGQVSCSSEELNAANTLKKAKQMTQIPAIEVIHRDSWVMVDQIKEEFQ
>Mature_372_residues
MNKKRVVIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLGYSSRPVTLKGKQASAAIGQG
LLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTMLELLERGVIPIINENDTVAVDELTFGDNDMLSALVSGSIH
AAQLIILTDIDGIYDKHPGKYASAMRYDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSK
LLEIVNGHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAGVINTQGDFEDGDVVEVYNRR
NLLGRGQVSCSSEELNAANTLKKAKQMTQIPAIEVIHRDSWVMVDQIKEEFQ

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=30.4195804195804, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=30.0699300699301, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786437, Length=319, Percent_Identity=35.7366771159875, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI17569967, Length=276, Percent_Identity=34.0579710144928, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=276, Percent_Identity=34.0579710144928, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=354, Percent_Identity=38.4180790960452, Blast_Score=186, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6321822, Length=353, Percent_Identity=32.2946175637394, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=32.8621908127208, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_OCEIH (Q8CUQ5)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691973.1
- ProteinModelPortal:   Q8CUQ5
- SMR:   Q8CUQ5
- GeneID:   1016930
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1052
- NMPDR:   fig|221109.1.peg.1054
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   OIHE221109:OB1052-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40618; Mature: 40618

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKRVVIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCC
YSSRPVTLKGKQASAAIGQGLLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTM
CCCCCEEEECCCHHHHHCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
LELLERGVIPIINENDTVAVDELTFGDNDMLSALVSGSIHAAQLIILTDIDGIYDKHPGK
HHHHHCCCCEEECCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCHHCCCCCC
YASAMRYDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSK
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
LLEIVNGHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAG
HHHHHCCCCCCEEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEECC
VINTQGDFEDGDVVEVYNRRNLLGRGQVSCSSEELNAANTLKKAKQMTQIPAIEVIHRDS
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
WVMVDQIKEEFQ
EEEHHHHHHHCC
>Mature Secondary Structure
MNKKRVVIKIGSSSLTNEKGEIDHKKLGDHVNALAMLHQHNFEVILVSSGAVAAGFRNLG
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHCC
YSSRPVTLKGKQASAAIGQGLLIHTYMDKFMEYNIRSAQLLLTRSDFSVQKRYKNATSTM
CCCCCEEEECCCHHHHHCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHH
LELLERGVIPIINENDTVAVDELTFGDNDMLSALVSGSIHAAQLIILTDIDGIYDKHPGK
HHHHHCCCCEEECCCCEEEEEEEECCCHHHHHHHHCCCCCEEEEEEEECCCCHHCCCCCC
YASAMRYDTIDLITNDMIQETDVSGSKLGTGGMKSKLMAAKVAASLGVPVFIGKGVGVSK
HHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
LLEIVNGHGQGTYVRSFRNKQIPIRKQWISLHSTLEGKVFIDDGATQALMHNGGSLLSAG
HHHHHCCCCCCEEEHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEECC
VINTQGDFEDGDVVEVYNRRNLLGRGQVSCSSEELNAANTLKKAKQMTQIPAIEVIHRDS
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
WVMVDQIKEEFQ
EEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376