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The map label for this gene is phoA [H]

Identifier: 23098484

GI number: 23098484

Start: 1068442

End: 1069773

Strand: Direct

Name: phoA [H]

Synonym: OB1029

Alternate gene names: 23098484

Gene position: 1068442-1069773 (Clockwise)

Preceding gene: 23098483

Following gene: 23098485

Centisome position: 29.43

GC content: 36.34

Gene sequence:

>1332_bases
GTGTTAAAGAAGGTAGGATGGATTTCAGTAATAAGTCTATTAATCTTTGCAGGAATTATGGGAGCATCTTTTGGTAACGA
AGAAGATGCAACATCAGCAAAGGGTAATAATAAAAAAGTAGAGAATGTAATATATATGATTCCAGATGGATTTAGTTCAG
ATTATGCTTCTAATTACCGTGCCTATAAAGGAGAAGAAGTAGTATGGGATTCTCACTTAAAAGGTCTTTTTACAACACAT
TCAGCAGATTCAGATGTTACTGATTCGGCAGCAGCCGGCACAGCAATGGCTACGGGTGAGAAAACAAACAATGGTGTTAT
TGGTAAAGATACGAATGGGAATAATTTAGAAACTATTTTAGAAAAATCCAAACAACATCATAAAGCAACCGGATTAGTAG
CTACATCAACAATTACACACGCAACTCCAGCTGCATTTGCAACCCATGTAGAAGATAGAAATAATGAAACGGAGATTGCA
CGACAATTAATTAAATCTGAAGTGGATGTTTTACTTGGTGGAGGAATGAACAAATTTATTGGCGAAGAAGAAGGCGGTAA
TCAAGAAGATGGTGAGCTAATGCAAAAGGCTCAAGATACGGGCTATATCTTAGCTGAAAATAAACAGGAAATGCTAGAAC
AAAATATAGATATCAAGAAAGACGAAAAATTACTTGGCTTGTTTGCAGATGAAGCGTTATCTCCAGAACTTCATAGAAAA
GATACGGAAGAACCAAGTTTAGAGGACATGACTACACATGCGATTGATCAACTGAATCAGAATAAAAAAGGATTTTTCTT
AATGGTGGAAGGTAGTCAAATTGATTGGGCTGGTCATGATAATGATTCTGCCTATGCGATGAGTGATACTGAAGCATTTG
AAAAAGCAGTTCAAGCTGCAATTGATTTTGCTGAAGAGGATGGAGAAACATTAGTTGTAGTTGCGGCTGATCATGATACA
GGAGGAATGACAACTGGTGGATATGACCAGATGGAATTAAATGCAAACATTCTTAATGATGTAACAGCAACTGGAGAATA
TATGGCTAATCAACTAGATGAAGAGCGTTCTAATGTCCATGAAGTCGTAAATACGTATACAGGATTTAAATTGACAGAAG
AAGAAATAGAATCTATTGAAACAGCAGAAGATCCAAAACTTGCTATCAATCATGTCGTTAGTGCACGATCTACCATTGGT
TGGACAAGTACTGGTCACACTGCTGCAGATATTCCGATTTATGCATATGGTCCAAGTGCAGAATCATTTTCTGGCAAATT
GGATAACACTGATATTCCAAAGTTAATAGAAAAGGCGATGAAATTAAAATAA

Upstream 100 bases:

>100_bases
TAAGGGTGTACTTTCTTCATTTGTTTTTAACTTTAGATTTATATTGAGTTAAACTTCCTTCGTTATGGTTGAAGTAGATA
AAAAAGAAGGAGGAATAGAT

Downstream 100 bases:

>100_bases
TAGTAGAAGGATTTAATCACTTTCATCAAAATGCTGTAAGTCCCCCAAAACTTATCGGTTTGATGTTCATTCCAGTCAAT
CTCTTTTAGTAGATTTTGCC

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MLKKVGWISVISLLIFAGIMGASFGNEEDATSAKGNNKKVENVIYMIPDGFSSDYASNYRAYKGEEVVWDSHLKGLFTTH
SADSDVTDSAAAGTAMATGEKTNNGVIGKDTNGNNLETILEKSKQHHKATGLVATSTITHATPAAFATHVEDRNNETEIA
RQLIKSEVDVLLGGGMNKFIGEEEGGNQEDGELMQKAQDTGYILAENKQEMLEQNIDIKKDEKLLGLFADEALSPELHRK
DTEEPSLEDMTTHAIDQLNQNKKGFFLMVEGSQIDWAGHDNDSAYAMSDTEAFEKAVQAAIDFAEEDGETLVVVAADHDT
GGMTTGGYDQMELNANILNDVTATGEYMANQLDEERSNVHEVVNTYTGFKLTEEEIESIETAEDPKLAINHVVSARSTIG
WTSTGHTAADIPIYAYGPSAESFSGKLDNTDIPKLIEKAMKLK

Sequences:

>Translated_443_residues
MLKKVGWISVISLLIFAGIMGASFGNEEDATSAKGNNKKVENVIYMIPDGFSSDYASNYRAYKGEEVVWDSHLKGLFTTH
SADSDVTDSAAAGTAMATGEKTNNGVIGKDTNGNNLETILEKSKQHHKATGLVATSTITHATPAAFATHVEDRNNETEIA
RQLIKSEVDVLLGGGMNKFIGEEEGGNQEDGELMQKAQDTGYILAENKQEMLEQNIDIKKDEKLLGLFADEALSPELHRK
DTEEPSLEDMTTHAIDQLNQNKKGFFLMVEGSQIDWAGHDNDSAYAMSDTEAFEKAVQAAIDFAEEDGETLVVVAADHDT
GGMTTGGYDQMELNANILNDVTATGEYMANQLDEERSNVHEVVNTYTGFKLTEEEIESIETAEDPKLAINHVVSARSTIG
WTSTGHTAADIPIYAYGPSAESFSGKLDNTDIPKLIEKAMKLK
>Mature_443_residues
MLKKVGWISVISLLIFAGIMGASFGNEEDATSAKGNNKKVENVIYMIPDGFSSDYASNYRAYKGEEVVWDSHLKGLFTTH
SADSDVTDSAAAGTAMATGEKTNNGVIGKDTNGNNLETILEKSKQHHKATGLVATSTITHATPAAFATHVEDRNNETEIA
RQLIKSEVDVLLGGGMNKFIGEEEGGNQEDGELMQKAQDTGYILAENKQEMLEQNIDIKKDEKLLGLFADEALSPELHRK
DTEEPSLEDMTTHAIDQLNQNKKGFFLMVEGSQIDWAGHDNDSAYAMSDTEAFEKAVQAAIDFAEEDGETLVVVAADHDT
GGMTTGGYDQMELNANILNDVTATGEYMANQLDEERSNVHEVVNTYTGFKLTEEEIESIETAEDPKLAINHVVSARSTIG
WTSTGHTAADIPIYAYGPSAESFSGKLDNTDIPKLIEKAMKLK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=363, Percent_Identity=30.0275482093664, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI94721246, Length=364, Percent_Identity=29.9450549450549, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157266292, Length=343, Percent_Identity=29.1545189504373, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI116734717, Length=466, Percent_Identity=27.0386266094421, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI294660770, Length=414, Percent_Identity=27.7777777777778, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI294660772, Length=411, Percent_Identity=27.7372262773723, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI48994877, Length=463, Percent_Identity=28.5097192224622, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320689, Length=472, Percent_Identity=33.4745762711864, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI19921912, Length=461, Percent_Identity=29.9349240780911, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859923, Length=424, Percent_Identity=27.3584905660377, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21355981, Length=458, Percent_Identity=28.1659388646288, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355149, Length=467, Percent_Identity=28.4796573875803, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21355151, Length=446, Percent_Identity=28.47533632287, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24651554, Length=349, Percent_Identity=33.5243553008596, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24657827, Length=470, Percent_Identity=29.7872340425532, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI85725200, Length=461, Percent_Identity=28.6334056399132, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI85815833, Length=461, Percent_Identity=28.6334056399132, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24651556, Length=327, Percent_Identity=33.3333333333333, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585213, Length=347, Percent_Identity=30.2593659942363, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24657842, Length=469, Percent_Identity=27.2921108742004, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21358067, Length=477, Percent_Identity=27.2536687631027, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24657835, Length=469, Percent_Identity=26.4392324093817, Blast_Score=123, Evalue=3e-28,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 48149; Mature: 48149

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKVGWISVISLLIFAGIMGASFGNEEDATSAKGNNKKVENVIYMIPDGFSSDYASNYR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECEEEEECCCCCCCHHHHCCC
AYKGEEVVWDSHLKGLFTTHSADSDVTDSAAAGTAMATGEKTNNGVIGKDTNGNNLETIL
CCCCCEEEEHHHCCCEEEECCCCCCCCCHHHCCCEEECCCCCCCCEECCCCCCCHHHHHH
EKSKQHHKATGLVATSTITHATPAAFATHVEDRNNETEIARQLIKSEVDVLLGGGMNKFI
HHHHHHHHHHCEEEECHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCHHHHC
GEEEGGNQEDGELMQKAQDTGYILAENKQEMLEQNIDIKKDEKLLGLFADEALSPELHRK
CCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHCC
DTEEPSLEDMTTHAIDQLNQNKKGFFLMVEGSQIDWAGHDNDSAYAMSDTEAFEKAVQAA
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCEEEECHHHHHHHHHHHH
IDFAEEDGETLVVVAADHDTGGMTTGGYDQMELNANILNDVTATGEYMANQLDEERSNVH
HHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EVVNTYTGFKLTEEEIESIETAEDPKLAINHVVSARSTIGWTSTGHTAADIPIYAYGPSA
HHHHHHCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCC
ESFSGKLDNTDIPKLIEKAMKLK
CCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLKKVGWISVISLLIFAGIMGASFGNEEDATSAKGNNKKVENVIYMIPDGFSSDYASNYR
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECEEEEECCCCCCCHHHHCCC
AYKGEEVVWDSHLKGLFTTHSADSDVTDSAAAGTAMATGEKTNNGVIGKDTNGNNLETIL
CCCCCEEEEHHHCCCEEEECCCCCCCCCHHHCCCEEECCCCCCCCEECCCCCCCHHHHHH
EKSKQHHKATGLVATSTITHATPAAFATHVEDRNNETEIARQLIKSEVDVLLGGGMNKFI
HHHHHHHHHHCEEEECHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCCHHHHC
GEEEGGNQEDGELMQKAQDTGYILAENKQEMLEQNIDIKKDEKLLGLFADEALSPELHRK
CCCCCCCCCHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHCC
DTEEPSLEDMTTHAIDQLNQNKKGFFLMVEGSQIDWAGHDNDSAYAMSDTEAFEKAVQAA
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCEEEECHHHHHHHHHHHH
IDFAEEDGETLVVVAADHDTGGMTTGGYDQMELNANILNDVTATGEYMANQLDEERSNVH
HHHHCCCCCEEEEEEECCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EVVNTYTGFKLTEEEIESIETAEDPKLAINHVVSARSTIGWTSTGHTAADIPIYAYGPSA
HHHHHHCCCEECHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCEECCEEEEECCCC
ESFSGKLDNTDIPKLIEKAMKLK
CCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]